Navigation Links
Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

at the peptide content reflects the protein level, a list of significantly and differentially expressed proteins was obtained after which identification of acquired MS/MS data was carried out.

In Figure 4, a screen capture of the PepMatch module of DeCyder MS is shown, covering the peptide LGMYPPNM representing the main olive allergen of these samples. The average ratio of this peptide between Ole_e1_"mer" and Ole_e1_"lite" was 3.8 times, as shown in the peptide table above the 2-D and 3-D signal intensity maps. This is also in agreement with the gel filtration data of the two allergen samples, which showed an average difference of 3.5 times after integration of the UV traces for the collected fractions.

LGMYPPNM was the only peptide that was identified in all Ole e 1 proteins identified in this study, resulting in a total quantitation of the allergens present in the two batches. This finding also reflects the fact that the most intense signals in DeCyder MS for all detected and matched Ole e 1 peptides were obtained for this peptide. From Figure 4 it can also be seen that signal intensities from singly as well as doubly charged forms of LGMYPPNM were used for calculation of the relative abundances of this specific peptide in the two batches of pollen. Also shown in the list above the 2-D and 3-D signal intensity maps are p values, identification details, molecular masses of the compared peptides, number of matched profiles, signal intensities of each matched peptide, etc.

Among other Ole e 1 peptides detected, some were found to be unique for different isoforms of the protein. In Figure 5, another view from PepMatch is shown, demonstrating a four-fold difference in abundance between Ole_e1_"mer" and Ole_e1_"lite" for the peptide DCDEIPIEGWAKPSLK. This peptide was found to be unique for the Ole e 1 protein, with a molecular mass of 16616 Da and accession number CAA73036.1. In total, five isoforms of Ole e 1 could
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Comparison of different protein quantitation methods
2. Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
3. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
4. Outstanding transfection rates using the Multiporator
5. Outstanding transfection rates using the Multiporator
6. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
7. Normalizing cDNA libraries using the EppendorfThermomixer
8. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
9. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
10. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
(Date:5/24/2013)... Texas (PRWEB) May 24, 2013 ... benchmarking survey on kinase profiling carried out in ... details of the breakdown of the responses for ... the future (2015). It also highlights some interesting ... survey groupings. Two bottom-up models were developed to ...
(Date:5/24/2013)... Many factors impact tissues targeting such as protein ... the blood-tissue barrier. Even when tissue disposition is achieved, ... tissue rather than the total tissue concentration. , A ... guide lead optimization, predict in vivo activity in animal ... impact the likely human clinical dose. , In this ...
(Date:5/24/2013)... City, OK (PRWEB) May 24, 2013 The ... of casualties. And following its ravage, the mayor of Moore, ... bill for new houses, said a report from cnn.com. Reaching ... the tornado victims complimentary Molderizer and Safe Shield to counter ... to the report published on May 22, 2013, caused 24 ...
(Date:5/24/2013)... Vestiage, Inc. (stock symbol "VEST") a publicly traded ... development, sales and marketing of premium branded highly ... it is awarding exclusive Vestiage territories in the ... Illuminating Facial Care. Qualified applicants can request an ... to info(at)vestiageinc(dot)com and putting RELUMA in the "Subject" ...
Breaking Biology Technology:Kinase Market Profiling - In House & Outsourced Trends 2015 Forecast in New Research Report at ReportsnReports.com 2Kinase Market Profiling - In House & Outsourced Trends 2015 Forecast in New Research Report at ReportsnReports.com 3Kinase Market Profiling - In House & Outsourced Trends 2015 Forecast in New Research Report at ReportsnReports.com 4DMPK for Targeted Tissue Delivery: Solutions for the Most Challenging Part of Outsourcing, New Life Science Webinar Hosted by Xtalks 2Mayor of Oklahoma-Tornado-Impaired Town Proposes Storm Shelter Bill, My Cleaning Products Offers Complimentary Molderizer and Safe Shield to Help Victim with Looming Mold 2Vestiage Announces Launch of Exclusive Territories for Reluma Brand Sales in USA 2Vestiage Announces Launch of Exclusive Territories for Reluma Brand Sales in USA 3Vestiage Announces Launch of Exclusive Territories for Reluma Brand Sales in USA 4Vestiage Announces Launch of Exclusive Territories for Reluma Brand Sales in USA 5
... how big a software project was, and we will often ... have units of time. How big was another 11,000-hour project ... 11,000-hour project that produced only a little bitof software, but ... software size, but without a measurable ounce of software, we're ...
... is the 10th in a series of articles on developing ... , Madison, Wis. - In Step 9 ... we focus specifically on the federal laws. , ,Any ... state and federal securities laws and regulations. Of equal importance ...
... adoption of healthcare information technology continues its slow but steady march, ... ,The progress that has been made, and the issues still to ... Conference set for May 9 and 10 at the Fluno ... , Dr. Barry Chaiken Dr. Barry Chaiken, associate chief ...
Cached Biology Technology:Defining the X-factor: What is an ounce of software worth? 2Defining the X-factor: What is an ounce of software worth? 3Defining the X-factor: What is an ounce of software worth? 4Early Stage 10: The nuances of federal laws 2Early Stage 10: The nuances of federal laws 3Early Stage 10: The nuances of federal laws 4Early Stage 10: The nuances of federal laws 5Digital Healthcare Conference 2007: Mounting obstacles for health IT 2Digital Healthcare Conference 2007: Mounting obstacles for health IT 3
(Date:5/23/2013)... , Automated conserved noncoding sequence (CNS) ... among grasses , Within the genome of each ... undergo little change in position and sequence over millions ... Some of these evolutionarily stable sequences, so-called conserved noncoding ... other genes or the condensation of chromosomes, but the ...
(Date:5/23/2013)... most popular fruit, can be made both better-tasting and ... , "Working with GM tomatoes that are different ... specific compound, allows us to pinpoint exactly how to ... the John Innes Centre. , The research could also ... shelf life characteristics because even higher levels of the ...
(Date:5/23/2013)... Pacific Northwest National Laboratory honored more than 165 ... intellectual property at PNNL,s annual Intellectual Property Commercialization ... Department of Energy national laboratory named materials scientist ... work developing battery materials that can store large ... grid, and reduce the time it takes to ...
Breaking Biology News(10 mins):Frontiers news briefs: May 23 2Frontiers news briefs: May 23 3The world's favorite fruit only better-tasting and longer-lasting 2PNNL staff recognized for scientific accomplishments, moving technologies into the marketplace 2
... SALT LAKE CITYLeukemia is the most common childhood cancer; ... zebrafish at Huntsman Cancer Institute (HCI) at the University ... in T-cell acute lymphocytic leukemia (T-ALL), according to an ... research journal Oncogene . When compared to samples ...
... Boys and girls have put many of the trappings of teenagerhood ... least some of the brain resculpting that characterizes the decade of ... findings in a new study that measured brainwaves of subjects in ... the kinds of neurological changes underway in a teen brain is ...
... at Washington State University Spokane offers hope to those ... which was published online this week in the journal ... nerve damage incurred during childhood can improve with intensive ... there isn,t just a one-year window for facial rehabilitation, ...
Cached Biology News:Zebrafish models identify high-risk genetic features in leukemia patients 2Study: Pace of brain development still strong in late teens 2WSU study expands time window for facial nerve rehabilitation 2
The LAS-3000 imaging system combines new CCD camera technology with a simplified user interface to provide significantly improved system sensitivity with a wide range of modularities. This system is...
This kit is designed to select cells labeled with biotinylated antibodies. Kit includes reagents for labeling 3 x 10 9 cells and EasySep magnet....
This pump accomodates StemSep ; pump feed columns of the following sizes : 0.3in., 0.5in., 0.6in., and 1.0in.....
... assays. The TUNEL-based assay kit provides complete ... for convenient detection of DNA fragmentation in ... offers an one-step labeling of apoptotic cells. ... hallmark of apoptosis in mammalian cells. MBLs ...
Biology Products: