Navigation Links
Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

using the PepDetect module of DeCyder MS, where features are detected and quantitated, background intensities are subtracted, and charge states are assigned. Furthermore, the LC-MS data is presented as two-dimensional intensity maps with m/z on the y axis and retention time (RT) on the x axis, as shown in Figure 3. The density of the grayscale pattern confined within the blue boxes is proportional to the signal intensity of a peptide peak at a certain m/z, RT, and charge state, and thus corresponds to full-scan MS data. The red crosses indicate positions (scan numbers) where MS/MS data is available that can be used for identification.

The intensity map in Figure 3 is derived from the same LC-MS data set that generated the base peak chromatogram shown in Figure 2. By comparing the two figures it is clear that the presentation of MS data in the form of an intensity map gives a much better overview of the LC-MS part of the analysis. For example, the signal intensity map clearly shows isotopic distribution of detected peptides, exact retention times, overlapped peptides, and the presence of MS/MS data not directly visible in a base peak ion chromatogram. This type of visualization thus results in a quick overview of the data and can be used to optimize chromatographic and MS conditions, observe unique patterns within the data, or recognize contaminants. At least 1000 ions were detected in each LC-MS analysis of the two allergen samples, which after applying the PepDetect module of DeCyder MS resulted in a final list consisting of ~ 500 deconvoluted and identified peptides.

The next step in the DeCyder MS software analysis was the matching of peptides, from different intensity maps derived from the fractions of Ole_e1_"lite" and Ole_e1_"mer," using the PepMatch module. In this module features from the two allergen samples were compared resulting in relative quantitation of significantly varying peptides. By assuming th
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Comparison of different protein quantitation methods
2. Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
3. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
4. Outstanding transfection rates using the Multiporator
5. Outstanding transfection rates using the Multiporator
6. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
7. Normalizing cDNA libraries using the EppendorfThermomixer
8. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
9. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
10. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
(Date:10/1/2014)... 2014 The U.S. Department of Labor ... University Edwardsville as part of the Trade Adjustment Assistance ... is co-administered by the Department of Labor and Department ... Labor Thomas E. Perez and Secretary of Education Arne ... training grants to nearly 270 colleges across the country. ...
(Date:9/30/2014)... WASHINGTON D.C., September 30, 2014 Applying a ... of researchers at Harvard University has demonstrated the ... surface -- work that holds promise for making ... and detailing the sides of next-gen rocket ships ... When light passes through oil in water, it ...
(Date:9/30/2014)... Sept. 30, 2014 Report Details ... highest potential revenues Do you want to find ... you revenue predictions for those biological drugs from ... you find financial data, R&D trends, opportunities and ... sales forecasts to 2024 at overall world market, ...
(Date:9/30/2014)... (PRWEB) September 30, 2014 Proove ... medicine, is excited to announce a new genetic test ... The Proove Pain Perception Test will provide physicians ... and why pain tolerance levels are stratified between individuals. ... identify whether a person has a genetic predisposition that ...
Breaking Biology Technology:U.S. Dept. of Labor Awards SIUE Nearly $10-Million Grant 2U.S. Dept. of Labor Awards SIUE Nearly $10-Million Grant 3U.S. Dept. of Labor Awards SIUE Nearly $10-Million Grant 4Taking thin films to the extreme 2Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 2Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 3Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 4Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 5Proove Biosciences is Now Offering the Proove Pain Perception Genetic Test to Help Physicians Assess Patient Pain Tolerance Levels 2Proove Biosciences is Now Offering the Proove Pain Perception Genetic Test to Help Physicians Assess Patient Pain Tolerance Levels 3
... Six new stem cell therapies, all forecast to reach physicians ... Summit on February 16 in New York City. These new therapies ... at least one FDA-approved stem cell drug. New studies showing rehydration ... are also on the agenda. , The Stem Cell Summit is ...
... REHOVOT, Israel, December 31 D-Pharm Ltd ... its,Phase III clinical study of DP-b99 (MACSI). The first patient ... trial involves,numerous medical centers in the US, Canada, Europe, Israel, ... product developed,for protection of brain cells suffering from restricted blood ...
... ... For The Massachusetts Biotechnology Council , ... Boston, MA (PRWEB) December 31, 2009 -- Social Media ... Biotechnology Council’s (MassBio) has selected the Boston-based Social Media Marketing Agency to plan and ...
Cached Biology Technology:5th Annual Summit to Feature New Stem Cell Therapies 2D-Pharm (TASE: DPRM) Announces Enrollment of First Patient in DP-b99 Phase III Efficacy Study, MACSI 2D-Pharm (TASE: DPRM) Announces Enrollment of First Patient in DP-b99 Phase III Efficacy Study, MACSI 3 The Massachusetts Biotechnology Council Selects Social Media Marketing Agency 451 Marketing To Develop A Social Media Strategy For 2010 2 The Massachusetts Biotechnology Council Selects Social Media Marketing Agency 451 Marketing To Develop A Social Media Strategy For 2010 3
(Date:9/30/2014)... the Mediterranean each year, but its deep-blue waters ... Almost 1,000 alien species, including fish, crustaceans, and ... human activities. In the open-access journal Frontiers ... researchers analyzed data from a new information system ... the introduction of alien species has changed the ...
(Date:9/30/2014)... --- Do we think of nature as something that we ... need to "preserve?" Or do we think of ourselves as ... of nature, but what about a house? , The ... also reflected in our actions, our speech and in cultural ... the University of Washington, the American Indian Center of Chicago ...
(Date:9/30/2014)... 2014--Sea monkeys have captured the popular attention of both ... life cycle -- sold as dehydrated eggs, these tiny ... more than a tank of salt water. , ... other zooplankton, brine shrimp vertically migrate in large groups ... the surface at night and retreating deeper during the ...
Breaking Biology News(10 mins):Biodiversity in the Mediterranean is threatened by alien species 2The cultural side of science communication 2Laser-guided sea monkeys show how zooplankton migrations may affect global ocean currents 2
... After a decade and a half of near stagnation, ... chronic illness instead of a terminal disease, a leading Colorado ... drugs have different and innovative methods of action. One is ... another is radiotherapy; and the final one is the first ...
... ingredient in the food industry because of its association with ... the February issue of the Journal of Food Science ... fish oil can be added to goat cheese to deliver ... or shelf-life. Fish oil delivers higher levels and more ...
... Republic of Congo has formally expanded Nouabal-Ndoki National Park ... most pristine forests and a population of "nave" chimpanzees ... apes investigate the conservationists who study them rather than ... 100-plus square-mile dense swamp forest and its unique great ...
Cached Biology News:4 new drugs will change prostate cancer care 2Great news for chimpanzees 2Great news for chimpanzees 3
... the unique quantitative and qualitative capabilities of ... TRAP LC/MS/MS Systems with the new Tempo ... provide a rapid solution for high sensitivity ... Eliminate extensive sample preparation with high instrument ...
... legendary MSn performance of the LTQ, incorporating ... Thermo Scientifics innovations in ion trap technology. ... only mass spectrometer that offers multiple dissociation ... Q Collision Induced Dissociation (PQD) is a ...
... of Agilent analytical solutions is the 6890N ... (LAN) allows you to share business and ... decision making. Just as its 6890 GC ... and performance needed for research and method ...
... Varian, Inc.'s CP-4900 is a ... always delivers the information you ... When the composition of gas ... generation micro-Gas Chromatograph generates more ...
Biology Products: