Navigation Links
Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

using the PepDetect module of DeCyder MS, where features are detected and quantitated, background intensities are subtracted, and charge states are assigned. Furthermore, the LC-MS data is presented as two-dimensional intensity maps with m/z on the y axis and retention time (RT) on the x axis, as shown in Figure 3. The density of the grayscale pattern confined within the blue boxes is proportional to the signal intensity of a peptide peak at a certain m/z, RT, and charge state, and thus corresponds to full-scan MS data. The red crosses indicate positions (scan numbers) where MS/MS data is available that can be used for identification.

The intensity map in Figure 3 is derived from the same LC-MS data set that generated the base peak chromatogram shown in Figure 2. By comparing the two figures it is clear that the presentation of MS data in the form of an intensity map gives a much better overview of the LC-MS part of the analysis. For example, the signal intensity map clearly shows isotopic distribution of detected peptides, exact retention times, overlapped peptides, and the presence of MS/MS data not directly visible in a base peak ion chromatogram. This type of visualization thus results in a quick overview of the data and can be used to optimize chromatographic and MS conditions, observe unique patterns within the data, or recognize contaminants. At least 1000 ions were detected in each LC-MS analysis of the two allergen samples, which after applying the PepDetect module of DeCyder MS resulted in a final list consisting of ~ 500 deconvoluted and identified peptides.

The next step in the DeCyder MS software analysis was the matching of peptides, from different intensity maps derived from the fractions of Ole_e1_"lite" and Ole_e1_"mer," using the PepMatch module. In this module features from the two allergen samples were compared resulting in relative quantitation of significantly varying peptides. By assuming th
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Comparison of different protein quantitation methods
2. Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
3. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
4. Outstanding transfection rates using the Multiporator
5. Outstanding transfection rates using the Multiporator
6. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
7. Normalizing cDNA libraries using the EppendorfThermomixer
8. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
9. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
10. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
(Date:7/23/2014)... 23, 2014  Having the right people at the ... accelerating business growth and achieving clinical and operational goals. ... Leadership Summit, July 20-22, 2014, in San ... executives and healthcare experts discussing how partnerships have positively ... President & Chief Executive Officer of Cape Regional Medical ...
(Date:7/23/2014)... DES MOINES, Iowa (PRWEB) July 23, 2014 ... will join the business as vice president of Agricultural ... from Mendel Biotechnology where he served most recently as ... brings extensive biotechnology and business leadership in the seed ... DuPont Pioneer,” said Paul E. Schickler , president ...
(Date:7/23/2014)... 23, 2014  GrowBLOX Sciences, Inc. (OTCQB: GBLX), a medical ... filing for two medical marijuana dispensary locations in ... to the Clark County, Nevada dispensary ... as previously announced.  GrowBLOX Sciences, CEO Craig ... new applications for dispensaries in the City of ...
(Date:7/23/2014)... (PRWEB) July 23, 2014 OncLive® ... Cancer Center has joined its Strategic Alliance Partnership ... Alliance Partnership program, UNC Lineberger will have the ... raise awareness of its cutting-edge research initiatives, community-directed ... UNC Lineberger will work with OncLive to educate ...
Breaking Biology Technology:MSC and Cape Regional Medical Center Partner to Improve Cost, Quality and Outcomes 2DuPont Pioneer Names Neal Gutterson to Lead Agricultural Biotechnology 2DuPont Pioneer Names Neal Gutterson to Lead Agricultural Biotechnology 3GrowBLOX Sciences, Inc. Files for Two Additional Medical Marijuana Dispensaries in Las Vegas 2GrowBLOX Sciences, Inc. Files for Two Additional Medical Marijuana Dispensaries in Las Vegas 3OncLive Expands Its Strategic Alliance Partnership Program With the Addition of UNC Lineberger Comprehensive Cancer Center 2OncLive Expands Its Strategic Alliance Partnership Program With the Addition of UNC Lineberger Comprehensive Cancer Center 3
... Repligen Corporation (Nasdaq: RGEN ) announced today that it will ... Wednesday, June 9th, 2010 , before the market opens.  The Company President ... conference call and webcast on the same day at 10:00 a.m. EDT ... Company. , , , ...
... June 2 /PRNewswire-Asia-FirstCall/ -- Huiheng Medical,Inc. ("Huiheng" or the ... an agreement to acquire all of the existing and,outstanding shares of ... consisting of the rights to develop,manufacture and market the ClearPath (TM) ... , , ...
... 2 DATATRAK International, Inc. (OTCQX: DATA), a technology and services ... announced its attendance at the Drug Information Association,s 46th Annual Meeting, ... Washington DC June 13-17 . , ... DATATRAK will be exhibiting at Booth #2435 during ...
Cached Biology Technology:Repligen Announces Conference Call of Fourth Quarter and Fiscal Year 2010 Financial Results 2Repligen Announces Conference Call of Fourth Quarter and Fiscal Year 2010 Financial Results 3Huiheng Medical, Inc. Announces Agreement to Acquire the ClearPath (TM) Breast Brachytherapy System 2Huiheng Medical, Inc. Announces Agreement to Acquire the ClearPath (TM) Breast Brachytherapy System 3Huiheng Medical, Inc. Announces Agreement to Acquire the ClearPath (TM) Breast Brachytherapy System 4Huiheng Medical, Inc. Announces Agreement to Acquire the ClearPath (TM) Breast Brachytherapy System 5DATATRAK Exhibiting at the DIA's 46th Annual Meeting 2DATATRAK Exhibiting at the DIA's 46th Annual Meeting 3
(Date:7/23/2014)... world by 2050, according to one of the scenarios ... may be advantageous to the physiology and the biochemical ... plants such as Stylosanthes capitata Vogel , a ... as Brazil., The conclusion is from a study carried ... the Ribeiro Preto Faculty of Philosophy, Sciences and Languages ...
(Date:7/23/2014)... 2014 BCC Research ... GLOBAL MARKETS AND TECHNOLOGIES FOR SENSORS , the global ... grow to nearly $154.4 billion by 2020, with a ... image, flow, and level sensors segment is moving at ... http://photos.prnewswire.com/prnh/20140723/694805 ) , Sensors have become indispensable ...
(Date:7/23/2014)... such as tapeworms, hookworms and a protist called Blastocystis ... new paper that argues we should rethink our views of ... the point, paper co-author Julius Luke even ingested three developmental ... latum . After more than a year with the tapeworms, ... metres each by now, he says he feels fine. , ...
Breaking Biology News(10 mins):An increase in temperature by 2050 may be advantageous to the growth of forage plants 2An increase in temperature by 2050 may be advantageous to the growth of forage plants 3An increase in temperature by 2050 may be advantageous to the growth of forage plants 4Global Market for Sensors to Reach Nearly $154.4 Billion in 2020; Image Sensors Moving at 11.7% 2Global Market for Sensors to Reach Nearly $154.4 Billion in 2020; Image Sensors Moving at 11.7% 3Intestinal parasites are 'old friends,' researchers argue 2Intestinal parasites are 'old friends,' researchers argue 3
... release is available in Spanish . , ... at fending off the aging process, known as senescence. To ... plant physiologist Cai-Zhong Jiang is investigating the gene-controlled mechanisms of ... modify flowers, aging-linked genes, or the proteins that are products ...
... Motor Neurone Disease Association is funding its first ever ... of this fatal, neurological condition. Pulling together ... and Prof Sir Ian Wilmut from the University of ... Prof Tom Maniatis of Columbia University New York; the ...
... North American small-mammal communities is so out of whack from ... that the current climate change could push them past a ... say Stanford biologists. The evidence lies in fossils spanning the ... in Northern California. What they found is that although ...
Cached Biology News:What genes help blossoms last longer? 2Harnessing the power of stem cells to unlock the secrets of motor neuron disease 2Harnessing the power of stem cells to unlock the secrets of motor neuron disease 3Harnessing the power of stem cells to unlock the secrets of motor neuron disease 4Small mammals -- and rest of food chain -- at greater risk from global warming than thought 2Small mammals -- and rest of food chain -- at greater risk from global warming than thought 3
... dotLab System brings together two mature, ... immobilized capture surfaces. This combination produces ... for the detection of molecular binding ... labels. , Protein-specific capture molecules are ...
... polymers) are prepared by ... the enzyme, polynucleotide phosphorylase, ... the appropriate, ribonucleoside diphosphate ... exception of catalog number ...
... provides a comprehensive experiment based analysis package ... menus yet sophisticated and powerful functionality., Image ... CCD cameras for easy image capture. ... Digital Video Playback Saturation Warning ...
...
Biology Products: