Navigation Links
Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

using the PepDetect module of DeCyder MS, where features are detected and quantitated, background intensities are subtracted, and charge states are assigned. Furthermore, the LC-MS data is presented as two-dimensional intensity maps with m/z on the y axis and retention time (RT) on the x axis, as shown in Figure 3. The density of the grayscale pattern confined within the blue boxes is proportional to the signal intensity of a peptide peak at a certain m/z, RT, and charge state, and thus corresponds to full-scan MS data. The red crosses indicate positions (scan numbers) where MS/MS data is available that can be used for identification.

The intensity map in Figure 3 is derived from the same LC-MS data set that generated the base peak chromatogram shown in Figure 2. By comparing the two figures it is clear that the presentation of MS data in the form of an intensity map gives a much better overview of the LC-MS part of the analysis. For example, the signal intensity map clearly shows isotopic distribution of detected peptides, exact retention times, overlapped peptides, and the presence of MS/MS data not directly visible in a base peak ion chromatogram. This type of visualization thus results in a quick overview of the data and can be used to optimize chromatographic and MS conditions, observe unique patterns within the data, or recognize contaminants. At least 1000 ions were detected in each LC-MS analysis of the two allergen samples, which after applying the PepDetect module of DeCyder MS resulted in a final list consisting of ~ 500 deconvoluted and identified peptides.

The next step in the DeCyder MS software analysis was the matching of peptides, from different intensity maps derived from the fractions of Ole_e1_"lite" and Ole_e1_"mer," using the PepMatch module. In this module features from the two allergen samples were compared resulting in relative quantitation of significantly varying peptides. By assuming th
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Comparison of different protein quantitation methods
2. Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
3. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
4. Outstanding transfection rates using the Multiporator
5. Outstanding transfection rates using the Multiporator
6. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
7. Normalizing cDNA libraries using the EppendorfThermomixer
8. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
9. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
10. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
(Date:6/19/2013)... , June 19, 2013 Biotechnology industry pioneer ... up with financial software provider BlackLine Systems  and ... SAP ) for a webinar next week entitled ... Solutions from BlackLine and SAP to Help Keep Its Account ... http://photos.prnewswire.com/prnh/20061117/LAF027LOGO ) The webinar, geared at ...
(Date:6/19/2013)... Austin, TX (PRWEB) June 19, 2013 ... new application report that details the measurement of sulfur ... NEX QC+ high resolution benchtop EDXRF analyzer . ... with standard test method ISO 13032. This International ... method for the determination of sulfur content in automotive ...
(Date:6/18/2013)... Clara, CA (PRWEB) June 18, 2013 ... to standard size SEMs but have limited performance and ... lower resolution. Full size SEMs would normally provide better ... more knowledge to operate and have a higher cost ... address the gap between these two types of ...
(Date:6/18/2013)... Los Angeles, CA (PRWEB) June 18, 2013 (PRWEB) ... of construction’s #1 time and attendance system in ... transfer software that shares employee data and payroll ... , AccountLinx Plus automates ExakTime software ... applications. The software imports employee data from a ...
Breaking Biology Technology:Amgen Joins BlackLine Systems, SAP for Webinar on Automating Account Reconciliations 2Amgen Joins BlackLine Systems, SAP for Webinar on Automating Account Reconciliations 3Amgen Joins BlackLine Systems, SAP for Webinar on Automating Account Reconciliations 4Rigaku Publishes New Application Note for Analysis of ULSD Per ISO 13032 2Nanounity Introduces the Pemtron Range of Compact Scanning Electron Microscopes 2Nanounity Introduces the Pemtron Range of Compact Scanning Electron Microscopes 3ExakTime Introduces AccountLinx Plus 2
... (Nasdaq: SGMO ) announced today that Edward Lanphier,Sangamo,s ... progress of,Sangamo,s ZFP Therapeutic development programs and an overview ... Tuesday, September 23,2008, at the 2008 UBS Global Life ... will be webcast live and may be accessed via ...
... ... the Genome, ST. LOUIS, Sept. 16 Sigma-Aldrich (Nasdaq:,SIAL) ... The CompoZr ZFN platform of reagents is expected to provide,researchers ... living cells, resulting in cell lines or whole organisms with ...
... lab supply ordering system that allows users to create ... currently order. ProSource then provides these items in one ... every order. ProSource eliminates the need to order from ... order from the same location, all while helping to ...
Cached Biology Technology:Sangamo BioSciences to Present at the UBS Global Life Sciences Conference 2Sigma-Aldrich Launches Breakthrough Genome-Editing Tools 2Sigma-Aldrich Launches Breakthrough Genome-Editing Tools 3ProSource 2.0 Offers Value, Savings for Lab Supplies 2
(Date:6/19/2013)... is extremely disappointed that the European Chemicals Agency ... that requires Dow Benelux B.V.to conduct a pre-natal ... use approximately 1160 animals and expose pregnant rats ... glycol methyl ether acetate (DPMA) in order to ... Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH). ...
(Date:6/19/2013)... In a scientific paper published in the journal Polar Biology, ... to study what may be a new type of killer ... a pod of unusual-looking killer whales stranded on a New ... in Wellington. Photographs were also taken but it was almost ... by a very small white eye-patch and bulbous forehead, was ...
(Date:6/19/2013)... 19, 2013 A decade-long JDRF-funded study led ... Zentrum Mnchen, Germany, is providing a deeper understanding ... of developing type 1 diabetes (T1D), highlighting the ... the disease. The study, "Seroconversion to Multiple Islet ... Children," was published today in The Journal ...
Breaking Biology News(10 mins):New data on islet autoantibodies in young children defines early type 1 diabetes development 2
... HILL, Mass. (4/15/08) A frequency-agile metamaterial that for ... of frequencies in the so-called terahertz gap has been ... Los Alamos National Laboratory and Boston University. The ... elements in this case metallic split-ring resonators ...
... Fla. University of Florida researchers have identified a gene ... the physical effects of mental stress to the point ... Searching for the presence of this gene may be one ... risk for the phenomenon, said David S. Sheps, M.D., a ...
... shook the Pacific atoll of Bikini, the corals are flourishing ... These are the findings of a remarkable investigation by ... and the Marshall Islands. The expedition examined the diversity ... One of the most interesting aspects is that the ...
Cached Biology News:Researchers bridge the 'terahertz gap' with new tunable metamaterial 2Researchers bridge the 'terahertz gap' with new tunable metamaterial 3Mental stress reduces blood flow to the heart in patients with gene variation 2Mental stress reduces blood flow to the heart in patients with gene variation 3Bikini corals recover from atomic blast 2Bikini corals recover from atomic blast 3
... Introduction of a protein into cells ... cell signaling pathways, apoptotic pathways, and transcription ... for studying the effects of a specific ... transfect the protein directly into the cell. ...
Reacti-Bind DNA Coating Solution...
Immunogen GST fusion protein corresponding to full-length rat JNK3/ SAPK1b. Quality Assurance Routinely evaluated by immunoblot. Stability 2 years at -20°C from date of shipment GenBank A...
... The ProteoExtract™ Protein Precipitation Kit ... up of proteins from aqueous samples. ... by the presence of non-protein impurities ... in the protein sample that interfere ...
Biology Products: