Navigation Links
Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

r," were subjected to a protein prefractionation step using Ettan LC System and gel filtration on Superdex 75 to reduce the complexity of the samples. Fractions of 0.5 ml were collected throughout the analyses. The overlaid chromatograms from the gel filtration analyses are shown in Figure 1.

Fractions A4–A6 were pooled because they contained proteins varying in size from 14 to 20 kDa, thus covering the expected molecular weight of Ole e 1 according to analyses by MALDI-ToF MS (data not shown). As can be seen from the chromatograms, Ole_e1_"mer" contained about 3.5 times more material in the collected fractions in comparison with Ole_e1_"lite," as judged by integration of the UV traces of the collected fractions.

Nanoscale LC-MS/MS of tryptic allergen peptides
Fractions A4–A6 from the gel filtration analyses of Ole_e1_"mer" and Ole_e1_"lite," respectively, were pooled, digested with trypsin, and analyzed by LC-MS/MS using the Ettan MDLC coupled to a Finnigan LTQ linear ion trap mass spectrometer. The resulting base peak ion chromatogram of Ole_e1_"lite" is shown in Figure 2.

The full scan MS data was acquired in profile mode, which is required in order to get enough data points for evaluation by DeCyder MS, while MS/MS data (not used for matching) was collected in centroid mode. The other batch, Ole_e1_"mer," displayed the same base peak chromatogram profile (data not shown). The generated MS and MS/MS files were transferred to TurboSequest software, which resulted in identification of about 40 proteins with at least two peptide hits for each protein from each of the examined samples. For each batch the highest ranked protein candidate was identified as the main olive allergen.

Detection of olive allergens using DeCyder MS
The individual intensity maps created from the Ettan MDLC-MS/MS analyzed fractions of Ole_e1_"mer" and Ole_e1_"lite" were examined
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Comparison of different protein quantitation methods
2. Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
3. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
4. Outstanding transfection rates using the Multiporator
5. Outstanding transfection rates using the Multiporator
6. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
7. Normalizing cDNA libraries using the EppendorfThermomixer
8. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
9. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
10. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
(Date:7/3/2015)... July 3, 2015 ... september 2015 på  http://www.openinnovationinscience.at till det ... Science" som hålls i Wien ... undersökning med internationella forskare och vetenskapsmän som ... hälsovetenskapens två största utmaningar bristen på incitament ...
(Date:7/3/2015)... 3, 2015  Pomerantz LLP announces that a class ... ("Puma" or the "Company")(NYSE: PBYI ) and certain ... States District Court, Central District of California ... of a class consisting of all persons or entities ... May 13, 2015 inclusive (the "Class Period").  This class ...
(Date:7/2/2015)... ... ... have been used in countless labs for decades. In the past, people had to ... expected, this was a daunting and tiring process looking at endless data tables and charts. ... navigate the complex catalog structure. , Now thanks to FireflySci, scientists are throwing away those ...
(Date:7/2/2015)... 2015  Spherix Incorporated (Nasdaq: SPEX ) -- an intellectual ... of intellectual property, today announced a litigation update. ... Verizon Services Corp. et al. , Case No. 1:14-cv-00721-GBL-TCB, ... District of Virginia , the Court ... certain key claims in favor of the Defendants on ...
Breaking Biology Technology:Lansering av världens första program för öppen innovation för att utbilda forskare 2Lansering av världens första program för öppen innovation för att utbilda forskare 3SHAREHOLDER ALERT: Pomerantz Law Firm Reminds Shareholders with Losses on their Investment in Puma Biotechnology, Inc. of Class Action Lawsuit and Upcoming Deadline - PBYI 2SHAREHOLDER ALERT: Pomerantz Law Firm Reminds Shareholders with Losses on their Investment in Puma Biotechnology, Inc. of Class Action Lawsuit and Upcoming Deadline - PBYI 3SHAREHOLDER ALERT: Pomerantz Law Firm Reminds Shareholders with Losses on their Investment in Puma Biotechnology, Inc. of Class Action Lawsuit and Upcoming Deadline - PBYI 4New Cuvette E-Commerce Website Launched by FireflySci 2Spherix Issues A Litigation Update 2Spherix Issues A Litigation Update 3
... (NYSE Amex: PTN ) will announce its first ... 2011 before the open of the U.S. financial markets. ... audio webcast hosted by its executive management team on November ... operations in greater detail and an update on corporate developments.Schedule ...
... but tweezers made of electrons. A recent paper* by researchers ... the University of Virginia (UVA) demonstrates that the beams produced ... look at nanoscale objects, but to move them around, position ... the tool is an electron version of the laser "optical ...
... Biopharmaceuticals, Inc. (Nasdaq: KERX ) today announced ... clinical observations from clinical trials of Zerenex™ (ferric citrate) ... American Society of Nephrology (ASN) Kidney Week 2011 Annual ... Friday, November 11, 2011 – ...
Cached Biology Technology:Palatin Technologies, Inc. to Report Fiscal Year 2012 First Quarter Results; Teleconference and Webcast to be held on November 15, 2011 2Are electron tweezers possible? Apparently so 2Keryx Biopharmaceuticals, Inc. Announces Upcoming Poster Presentations of Zerenex™ (Ferric Citrate) at the Upcoming American Society of Nephrology Kidney Week 2011 Annual Meeting 2Keryx Biopharmaceuticals, Inc. Announces Upcoming Poster Presentations of Zerenex™ (Ferric Citrate) at the Upcoming American Society of Nephrology Kidney Week 2011 Annual Meeting 3
(Date:6/15/2015)... -- According to a new market research ... and Hybrid), Technologies (Recognition, IVR, OCR, Pattern & Image ... The Natural Language Processing Market is expected to grow ... the forecast period 2015-2020. Browse 65 ... spread   through 155 P ages and in-depth ...
(Date:6/12/2015)... 2015  Securus Technologies, a leading provider of ... safety, investigation, corrections and monitoring, announced the United ... capture, analyze and identify participants of a video ... This system can: , Identify ... subsequent sign-on process is actually the person participating ...
(Date:6/9/2015)... 2015   MedNet Solutions , an innovative SaaS-based ... clinical research, will be providing demonstrations of its ... 2015 Drug Information Association (DIA) Annual Meeting in ... presentations will include previews of many exciting new enhancements ... iMedNet 2015 Feature 1 Release. ...
Breaking Biology News(10 mins):Natural Language Processing Market Worth $13.4 Billion by 2020 2Natural Language Processing Market Worth $13.4 Billion by 2020 3Natural Language Processing Market Worth $13.4 Billion by 2020 4Natural Language Processing Market Worth $13.4 Billion by 2020 5Securus Announces Patent Issued for Authorized Presence Verification During Video Visitation 2MedNet To Provide Sneak Peeks Of Its iMedNet "2015 Feature 1 Release" At The DIA Annual Meeting 2MedNet To Provide Sneak Peeks Of Its iMedNet "2015 Feature 1 Release" At The DIA Annual Meeting 3
... TexasPhysical anthropologist Chris Kirk has announced the discovery of a ... in the Devil,s Graveyard badlands of West Texas. ... million years ago, and would have most closely resembled a ... an extinct primate group the adapiforms that were ...
... physical abuse is associated with significantly elevated rates of ... and multiple chemical sensitivities among women, according to new ... be published in this month,s issue of the ... who reported they had been physically abused as children ...
... attracted the attention of ornithologists for a long time, mainly ... use to dispatch their prey. In the book, the most ... of which has accumulated in the ornithological literature and obtained ... the framework of an integral system of adaptation, are evolutionary ...
Cached Biology News:Anthropologist discovers new fossil primate species in West Texas 2Anthropologist discovers new fossil primate species in West Texas 3
... sequencing of individual research samples, including PCR ... Agencourt's world-class, high performance sequencing pipeline processes ... The key benefits of Agencourt's QuickLane ... 48 hour sample turnaround, industry leading sequence ...
A component of the Acridinium protein labeling kit....
... Affinity Life Sciences can provide custom microplate coating using ... washes a variety of plate formats. We can also accommodate ... volume range of 0.5-320 L):, , ... 96 well microplate, ...
... HRP Conjugation Kits are based on ... that are consistent and reproducible. This ... conjugate your protein or antibody, including ... buffer. Activated HRP is supplied ...
Biology Products: