Navigation Links
Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

r," were subjected to a protein prefractionation step using Ettan LC System and gel filtration on Superdex 75 to reduce the complexity of the samples. Fractions of 0.5 ml were collected throughout the analyses. The overlaid chromatograms from the gel filtration analyses are shown in Figure 1.

Fractions A4–A6 were pooled because they contained proteins varying in size from 14 to 20 kDa, thus covering the expected molecular weight of Ole e 1 according to analyses by MALDI-ToF MS (data not shown). As can be seen from the chromatograms, Ole_e1_"mer" contained about 3.5 times more material in the collected fractions in comparison with Ole_e1_"lite," as judged by integration of the UV traces of the collected fractions.

Nanoscale LC-MS/MS of tryptic allergen peptides
Fractions A4–A6 from the gel filtration analyses of Ole_e1_"mer" and Ole_e1_"lite," respectively, were pooled, digested with trypsin, and analyzed by LC-MS/MS using the Ettan MDLC coupled to a Finnigan LTQ linear ion trap mass spectrometer. The resulting base peak ion chromatogram of Ole_e1_"lite" is shown in Figure 2.

The full scan MS data was acquired in profile mode, which is required in order to get enough data points for evaluation by DeCyder MS, while MS/MS data (not used for matching) was collected in centroid mode. The other batch, Ole_e1_"mer," displayed the same base peak chromatogram profile (data not shown). The generated MS and MS/MS files were transferred to TurboSequest software, which resulted in identification of about 40 proteins with at least two peptide hits for each protein from each of the examined samples. For each batch the highest ranked protein candidate was identified as the main olive allergen.

Detection of olive allergens using DeCyder MS
The individual intensity maps created from the Ettan MDLC-MS/MS analyzed fractions of Ole_e1_"mer" and Ole_e1_"lite" were examined
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Comparison of different protein quantitation methods
2. Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
3. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
4. Outstanding transfection rates using the Multiporator
5. Outstanding transfection rates using the Multiporator
6. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
7. Normalizing cDNA libraries using the EppendorfThermomixer
8. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
9. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
10. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
(Date:6/18/2013)... Nasseo, Inc. today announces that it has been ... of applicants by the Arizona Commerce Authority (ACA) ... The Arizona Innovation Challenge (AIC) awards the most money ... a financial award to what it deems to be ... the art dental and orthopedic implant surface modification technology ...
(Date:6/18/2013)... 2013  regulated information — Global biopharmaceutical company UCB, ... announced the launch of a new Pledge campaign for ... The Pledge empowers the millions of Americans ... a personal commitment to demand more: greater understanding of ... and additional partial-onset seizure control.  More information is available ...
(Date:6/18/2013)... , June 18, 2013 The ... (Sequencing Platforms, Knowledge Management Tools & Data Analysis Services) & ... and studies the Major Market Drivers, Restraints, and Opportunities in ... , Asia-Pacific and Rest of World. ... Tables 22 Figures ...
(Date:6/18/2013)... This webinar will examine the ... promotion, and review the basic FDA regulations and ... apply these principles through real world case studies ... fundamentals on FDA regulations and guidance related to ...     Assessing traditional and newer forms of promotional materials ...
Breaking Biology Technology:Nasseo Named 2013 Innovation Challenge Winner by Arizona Commerce Authority 2Professional Golfer Jeff Klauk Tees Off New Pledge Campaign for People Living with Epilepsy 2Professional Golfer Jeff Klauk Tees Off New Pledge Campaign for People Living with Epilepsy 3Professional Golfer Jeff Klauk Tees Off New Pledge Campaign for People Living with Epilepsy 4Professional Golfer Jeff Klauk Tees Off New Pledge Campaign for People Living with Epilepsy 5Professional Golfer Jeff Klauk Tees Off New Pledge Campaign for People Living with Epilepsy 6Bioinformatics Market Worth $7.5 Billion by 2017 2Bioinformatics Market Worth $7.5 Billion by 2017 3Drug Advertising and Promotion Labeling: FDA Regulations, New Webinar Hosted by Xtalks 2
... Rayovac Corp. , a force in the Madison ... an Atlanta, Ga. suburb in May. , ,According to ... move south. The announcement came as a surprise to many. ... chance to try to work with [Rayovac], said Melanie Conklin, ...
... , ,With deregulation of the retail electric utility ... appears to be a time to return to regulated utility ... the original text for the regulation of public utilities to ... on energy policy states in WI State Statute 1.112 in ...
... Midwest technology job market these days is an exercise in faith. Every ... are truly turning around. , ,At the same time, lots of ... when we tell them about jobs we've spotted, they say to us: ... ChicWIT or Monster.com ) you wrote to me about but ...
Cached Biology Technology:Rayovac to Relocate Headquarters 2Wisconsin Should Create, like Renewables, an Energy Efficient Portfolio 2Wisconsin Should Create, like Renewables, an Energy Efficient Portfolio 3Wisconsin Should Create, like Renewables, an Energy Efficient Portfolio 4Pity the Midwest Technology Sales Guy 2Pity the Midwest Technology Sales Guy 3Pity the Midwest Technology Sales Guy 4Pity the Midwest Technology Sales Guy 5
(Date:6/19/2013)... birds are threatened with extinction as a result of rising ... The Ohio State University in the US. Their work is ... the human population will continue to pose a threat to ... be a threshold above which population growth would cease to ... in Springer,s journal, Human Ecology . , It has ...
(Date:6/19/2013)... June 19, 2013 A decade-long JDRF-funded study ... Helmholtz Zentrum Mnchen, Germany, is providing a deeper ... risk of developing type 1 diabetes (T1D), highlighting ... for the disease. The study, "Seroconversion to Multiple ... in Children," was published today in The ...
(Date:6/19/2013)... study offers further proof that the divergence of humans ... ago was profoundly influenced by mutations to DNA sequences ... , The study, published June 9 in Nature ... regulation of genes must play an important role in ... chimps in the proteins produced by genes. Indeed, human ...
Breaking Biology News(10 mins):Are we pushing animals over the edge? 2New data on islet autoantibodies in young children defines early type 1 diabetes development 2New research backs theory that genetic 'switches' play big role in human evolution 2New research backs theory that genetic 'switches' play big role in human evolution 3
... bacteria and tiny plants living in the ocean need iron ... than ours for one, they can,t exactly turn to ... a pick-me-up. So where does their iron come from? ... a source on the seafloor: minute particles (called nanoparticles) of ...
... had assumed that wild animals died before they got old. ... demonstrated ageing in a population of wild birds ( Sula ... reproduce. "It was always thought that senescence was something ... extended life expectancy", Alberto Velando, lead author of the study ...
... conducted at Washington State University Spokane offers hope to ... study, which was published online this week in the ... facial nerve damage incurred during childhood can improve with ... that there isn,t just a one-year window for facial ...
Cached Biology News:As good as gold 2Wild animals age too 2WSU study expands time window for facial nerve rehabilitation 2
4',6-Diamidino-2-phenylindole, dihydrochloride (DAPI)...
Includes PROTEAN II xi Basic unit and IPG conversion kit PROTEAN II XL Cell, wide format, Compatible with ReadyStrip IPG Strips 1.0 mm 165-3188 1.5 mm 165-3189 2.0 mm 165-3190...
... cell, 16 cm, is used for vertical slab ... agarose gels. The cell is supplied without spacers ... core with gaskets, lower buffer chamber, lid with ... clamps, upper buffer dam, casting stand with gaskets, ...
... This depletion cocktail is ... (CD3+CD4+CD8+) cells from suspensions of ... Cells are separated using the ... immunomagnetic negative selection system, achieving ...
Biology Products: