Navigation Links
Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

separated the sample. The mobile phases were A: HPLC-grade water with 0.1% formic acid, and B: 84% HPLC-grade acetonitrile with 0.1% formic acid. Approximately 0.50 µg of material was loaded onto the columns and separation was performed at a flow rate of 200 nl/min by applying a linear gradient of 0–60% B for 50 min. The digested samples were run in triplicate.

Mass spectrometry
A Finnigan LTQ linear ion trap mass spectrometer equipped with a nanospray interface was used as the detector for the peptides that eluted from the RPC column. Full scan mass spectra were collected in profile mode and MS/MS spectra in centroid mode.

Detection of significantly varying peptides using DeCyder MS
Detection, profile comparison, background subtraction, and quantitation were done on the full scan precursor mass spectra in fully automatic mode using DeCyder MS (1).

The PepDetect module of DeCyder MS was used for automated peptide detection, charge-state assignments, deconvolution, and quantitation based on MS signal intensities of individual LC-MS analyses.

The PepMatch module was used to match peptides in different intensity maps from the different runs, which resulted in a quantitative comparison including statistical evaluation. The background intensity was used for normalization (no internal standards were added to the samples). Peptides were identified using intact masses by exporting the MS/MS files into TurboSEQUEST protein identification software and subsequently importing TurboSEQUEST search results into the PepMatch module. The peptide matches were filtered based on cross-correlation scores (Xcorr) of 1.5, 2, and 2.5 for charge states 1+, 2+, and 3+, respectively.


Results and discussion
Protein prefractionation of allergen samples
The two allergen samples, Ole_e1_"lite" and Ole_e1_"me
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Comparison of different protein quantitation methods
2. Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
3. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
4. Outstanding transfection rates using the Multiporator
5. Outstanding transfection rates using the Multiporator
6. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
7. Normalizing cDNA libraries using the EppendorfThermomixer
8. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
9. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
10. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
(Date:8/27/2014)... YORK , Aug. 27, 2014 ... is available in its catalogue: Global ... http://www.reportlinker.com/p02316662/Global-Chelating-Agents-Market-2014-2018.html About Chelating Agent A ... form covalent bonds with metal ions, thereby forming ... that some metal-ions have on chemical processes, formulations, ...
(Date:8/27/2014)... 27, 2014 Guggenheim Securities, the investment banking ... the hiring of veteran equity analyst Charles "Tony" Butler ... Butler will focus on the biotech/biopharma sector. ... of experience and a broad network of relationships in ... Director and Head of Equities at Guggenheim Securities. "His ...
(Date:8/27/2014)... -- ARCH Venture Partners, one of the largest U.S. ... seed and early-stage advanced technology companies, today announced ... than $400 million in subscriptions. ARCH Venture Fund ... more than $150 million. "The enthusiasm ... of our approach to finding and funding new ...
(Date:8/27/2014)... (PRWEB) August 27, 2014 According to ... continues to reshape the look and feel of markets ... demand for higher-quality ingredients will push for more branded, ... market have experienced strong market growth in the last ... in their food and cosmetics to fit a more ...
Breaking Biology Technology:Global Chelating Agents Market 2014-2018 2Global Chelating Agents Market 2014-2018 3Global Chelating Agents Market 2014-2018 4Guggenheim Securities Hires Analyst Charles "Tony" Butler to Expand Healthcare Coverage 2Latest ARCH Venture Partners Fund Closes Above $400 Million 2Latest ARCH Venture Partners Fund Closes Above $400 Million 3Daring to be Different Raw Materials & Ingredient Supply Shortage Fuels Vital Force™ Technology New Ingredient Enhancements 2Daring to be Different Raw Materials & Ingredient Supply Shortage Fuels Vital Force™ Technology New Ingredient Enhancements 3
... ... Communications and Communications Convergence , ... San Diego, CA (PRWEB) June 17, 2009 -- CommNexus and ... of unlimited wireless services, today announce their support for EvoNexus, a non-profit incubator dedicated ...
... Calif., June 17 Oclaro, Inc. (Nasdaq: ... Fluorescence Filter for biomedical and analytical instrumentation markets. Oclaro ... range of biomedical and analytical applications, including clinical diagnostics, ... The latest offerings from the Oclaro filter portfolio are ...
... BioEnergy International, LLC (BioEnergy), a ... biochemicals and biofuels, announces the formation of Myriant ... biobased chemicals business and intellectual property. BioEnergy ... its 110 million gallon per day ethanol plant ...
Cached Biology Technology:San Diego Wireless Heavyweight Leap Wireless Supports New Start-up Incubator 2San Diego Wireless Heavyweight Leap Wireless Supports New Start-up Incubator 3San Diego Wireless Heavyweight Leap Wireless Supports New Start-up Incubator 4Oclaro(TM) Hits Milestone With Clarity(TM) Fluorescence Filters 2Oclaro(TM) Hits Milestone With Clarity(TM) Fluorescence Filters 3BioEnergy International, LLC Announces Formation of Myriant Technologies LLC 2
(Date:8/27/2014)... of fishing traps are lost or abandoned each year ... derelict traps, which continue to catch fish, crabs, and ... losses to habitat, fisheries, and the watermen who depend ... a newly published NOAA study. , The report, published ... first of its kind to examine the derelict fish ...
(Date:8/27/2014)... Complex of fires had consumed 24,939 acres of land ... as of eight hours ago. , Lightning strikes ... of the Klamath National Forest when a thunderstorm passed ... three of those fires are now 100 percent contained. ... their size at containment: Delta, 150 acres; Sutcliffe, 27 ...
(Date:8/26/2014)... a colony of harmful bacteria, biofilms make the ... in a biofilm pose a significant health risk ... treatment, and biofilm-protected bacteria account for some 80 ... are 50 to 1,000 times more resistant to ... we may have stumbled onto a magic bullet," ...
Breaking Biology News(10 mins):NOAA's Marine Debris Program reports on the national issue of derelict fishing traps 2Happy Camp and July Fire Complexes in California 2Breakthrough antibacterial approach could resolve serious skin infections 2Breakthrough antibacterial approach could resolve serious skin infections 3
... and American Board of Internal Medicine (ABIM) announce the ... performance evaluation tools for blood specialists. , ... ABIM certification, ASH and ABIM have co-developed the Hematology ... to complete their practice performance assessment requirement for recertification. ...
... research project which could revolutionise the diagnosis and treatment ... four-year project, the results of which are now being ... means of identifying the two most deadly forms of ... for practitioners. , The implications for improving children's health ...
... to ferns to create a novel energy scavenging device that ... provide a method for powering micro and nano devices with ... works," said Michel Maharbiz, assistant professor of electrical engineering and ... the device. "If you build these things they will move. ...
Cached Biology News:New tool helps blood specialists improve patient care 2Hope for major advance in fighting world killer disease 2Hope for major advance in fighting world killer disease 3Ferns provide model for tiny motors powered by evaporation 2
Tachykinin (H-2)...
Human BMP 2 Construction Kit provides antigen affinity purified polyclonal capture and tracer antibodies, and antigen standard for development of approximately ten microplate assays....
Rabbit Anti-Ferret Whole Serum Antibody, Unconjugated...
... utilizies CTI Molecular Imaging's proprietary Focus™ detector ... volume as the R4. The microPET ... any commerically avaiable PET scanner. Focus ... of any scinitallor based PETsystems. The ...
Biology Products: