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Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

different samples, Ole_e1_"mer" and Ole_e1_"lite," was determined using DeCyder MS.


Materials
Products used
DeCyder MS Differential 11-0013-32
Analysis Software
(including PC and single concurrent network user license)

Ettan MDLC 18-1176-44, 11-0008-41

Ettan LC System 18-5050-50

Fraction Collector Frac- 950 18-6083-00

Superdex™ 75 10/300 GL 17-5174-01

NAP™ 10 Columns 17-0854-02

Trypsin, sequencing grade 17-6002-75

PlusOne™ Dithiothreitol (DTT) 17-1318-01

PlusOne Tris 17-1321-01

PlusOne Urea 17-1319-01

PlusOne Iodoacetamide RPN6302


Other products
Finnigan™ LTQ™ mass spectrometer (Thermo Electron)

TurboSEQUEST™ protein identification software (Thermo Electron)

Zorbax™ 300-SB C18 trap column, 300 µm i.d. x 5 mm, 3 µm (Agilent)

Zorbax 300-SB C18 analytical column, 75 µm i.d. x 150 mm, 3 µm (Agilent)

Formic acid, ultrapure (Fluka)

Acetonitrile, HPLC grade

Water, HP
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