Navigation Links
Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

Key words: multidimensional liquid chromatography (MDLC) • reversed-phase chromatography (RPC) • LC-MS/MS • DeCyder MS • differential analysis


Ettan™ MDLC was used in conjunction with tandem mass spectrometry (MS/MS) to separate and identify allergenic proteins extracted from the pollen of the olive tree, Olea europaea. DeCyder™ MS Differential Analysis Software (DeCyder MS) was used to quantitate differentially expressed proteins in two different samples, Ole_e1_"mer" and Ole_e1_"lite."


Introduction
Ole e 1 is the most frequent sensitizing agent affecting more than 70% of the patients suffering from olive (Olea europaea) pollinosis. The protein consists of a single polypeptide chain of 145 amino acids (MW 16.3 kDa) and has been suggested to be involved in the hydration of pollen during germination (1).

Ole e 1 exists in several isoforms, displaying microheterogeneities, that are difficult to resolve by gel-based methods. Therefore, we used an LC-MS/MS protein separation and identification strategy.

DeCyder MS Differential Analysis Software (DeCyder MS) is a tool for visualization, detection, comparison, and label-free relative quantitation of LC-MS/MS data (2). In brief, the software displays LC-MS analyses as two-dimensional intensity maps, resulting in a much better overview of spectral quality and data compared with the traditional way to present LC-MS data. Statistical analysis is carried out on all peptide spots within minutes.

The DeCyder MS algorithm is based on accurate mass and reproducible retention times. Ettan MDLC is ideally suited to deliver reproducible retention times (3), and should be used to generate the data that will be analyzed by DeCyder MS.

In this application note, relative quantitation of allergenic proteins in two
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Comparison of different protein quantitation methods
2. Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
3. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
4. Outstanding transfection rates using the Multiporator
5. Outstanding transfection rates using the Multiporator
6. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
7. Normalizing cDNA libraries using the EppendorfThermomixer
8. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
9. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
10. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
(Date:5/24/2013)... BOSTON and TEL AVIV, Israel ... ("InspireMD" or the "Company") (NYSE MKT: NSPR), a leader in ... , the Company,s President and Chief Executive Officer, and ... will participate in the Benchmark Company, LLC One-on-One Investor Conference ... East Wisconsin Ave., Milwaukee, WI. ...
(Date:5/24/2013)... This market report summarizes the ... carried out in May 2013. The full report ... the responses for each question, its segmentation and ... highlights some interesting differences, particularly between Pharma, Biotech ... were developed to estimate the in house and ...
(Date:5/24/2013)... (PRWEB) May 24, 2013 The Oklahoma ... And following its ravage, the mayor of Moore, one of ... new houses, said a report from cnn.com. Reaching out to ... victims complimentary Molderizer and Safe Shield to counter the looming ... report published on May 22, 2013, caused 24 deaths, My ...
(Date:5/24/2013)... WI (PRWEB) May 24, 2013 ... the discovery and development of calcium-binding proteins to ... of safety assessment study of apoaequorin ... international journal published for the British Industrial Biological ... study was to investigate potential adverse effects, if ...
Breaking Biology Technology:InspireMD To Participate In Benchmark Investor Conference On May 30th 2InspireMD To Participate In Benchmark Investor Conference On May 30th 3InspireMD To Participate In Benchmark Investor Conference On May 30th 4Kinase Market Profiling - In House & Outsourced Trends 2015 Forecast in New Research Report at ReportsnReports.com 2Kinase Market Profiling - In House & Outsourced Trends 2015 Forecast in New Research Report at ReportsnReports.com 3Kinase Market Profiling - In House & Outsourced Trends 2015 Forecast in New Research Report at ReportsnReports.com 4Mayor of Oklahoma-Tornado-Impaired Town Proposes Storm Shelter Bill, My Cleaning Products Offers Complimentary Molderizer and Safe Shield to Help Victim with Looming Mold 2Safety Assessment Study of Apoaequorin Published in Food and Chemical Toxicology 2
... Six prestigious members of the National Institutes of ... ), the nation,s leading online jobs board and ... The NIH has the highest number of post-doctoral professionals ... NIH organizations that have joined include the National Institute ...
... WORTH, Texas, June 16 Wound Care Innovations, LLC, ... today that it has reached an agreement in principle ... biotechnology company with a platform of proprietary and patented ... The transaction would be structured as a share exchange ...
... , , ARVADA, Colo., June 16 GeneThera, ... responsibility to our shareholders, is addressing our recent events. ... 2009, a Biotechnology, Medical Devices, and world public and ... , , Pharma Finance is an international event ...
Cached Biology Technology:National Institutes of Health Join Nation's Leading Postgrad Bio Jobs Board 2National Institutes of Health Join Nation's Leading Postgrad Bio Jobs Board 3Wound Management Technologies, Inc. Announced Today That It Has Reached an Agreement in Principal to Acquire BioPharma Management Technologies, Inc. 2GeneThera Extends its Reach Overseas 2
(Date:5/24/2013)... recent years, microRNAs (miRNAs) and other non-coding RNAs ... of specific proteins. In recent years they have ... used to establish tissue origin for cancers of ... and screen for disease, but clinically tractable, diagnostic ... are not currently available. In this issue of ...
(Date:5/23/2013)... most popular fruit, can be made both better-tasting and ... , "Working with GM tomatoes that are different ... specific compound, allows us to pinpoint exactly how to ... the John Innes Centre. , The research could also ... shelf life characteristics because even higher levels of the ...
(Date:5/23/2013)... DNA around nucleosomes in the cell nucleus makes ... Ludwig-Maximilians-Universitaet (LMU) in Munich now describes a mechanism ... from nucleosomes for transcription. , In higher organisms ... nucleus, wrapped around disk-shaped particles called nucleosomes, each ... proteins and accommodating two loops of DNA. Packed ...
Breaking Biology News(10 mins):The world's favorite fruit only better-tasting and longer-lasting 2Biochemistry: Unspooling DNA from nucleosomal disks 2
... mating contests but,diminutive males make ten times better ... their feet, a new study of redback spiders,reveals. ... Journal of Evolutionary,Biology , the study shows ... success. In field enclosures, researchers,simulated two competitive contexts ...
... (Dec. 7, 2008) Rods and cones coexist peacefully in ... of tissue and send signals when they detect light, which ... Retinitis Pigmentosa, however, reveals a codependent relationship between the two ... otherwise normal cones follow them to the grave, leading to ...
... VIB researchers connected to the Katholieke Universiteit Leuven have discovered ... This happens in the same way − and with the ... a hormone. This finding can teach us more about how ... basis for new candidate targets for medicines. ...
Cached Biology News:Spider love: Little guys get lots more 2Progression of retinal disease linked to cell starvation 2Food can affect a cell in the same way hormones do 2
Carp Vitellogenin ELISA System, 96 wells. ELISARange: 5.0-225 ng/ml.Sensitivity: 5.0 ng/ml.Sample volume: 100 ul.Suitable for use with Carp plasma samples.3-h protocol.Store at 2-8 C. Category: Drug ...
Quartz-Halogen, 100 W, High Intensity...
... the translation boosting Cap 1 structure. mScript™ ... 40% to 80% capped using co-transcriptional systems) ... mScript™ also features the Cap 1 structure, ... to 50% more efficient in in vivo ...
...
Biology Products: