Navigation Links
Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

Key words: multidimensional liquid chromatography (MDLC) • reversed-phase chromatography (RPC) • LC-MS/MS • DeCyder MS • differential analysis


Ettan™ MDLC was used in conjunction with tandem mass spectrometry (MS/MS) to separate and identify allergenic proteins extracted from the pollen of the olive tree, Olea europaea. DeCyder™ MS Differential Analysis Software (DeCyder MS) was used to quantitate differentially expressed proteins in two different samples, Ole_e1_"mer" and Ole_e1_"lite."


Introduction
Ole e 1 is the most frequent sensitizing agent affecting more than 70% of the patients suffering from olive (Olea europaea) pollinosis. The protein consists of a single polypeptide chain of 145 amino acids (MW 16.3 kDa) and has been suggested to be involved in the hydration of pollen during germination (1).

Ole e 1 exists in several isoforms, displaying microheterogeneities, that are difficult to resolve by gel-based methods. Therefore, we used an LC-MS/MS protein separation and identification strategy.

DeCyder MS Differential Analysis Software (DeCyder MS) is a tool for visualization, detection, comparison, and label-free relative quantitation of LC-MS/MS data (2). In brief, the software displays LC-MS analyses as two-dimensional intensity maps, resulting in a much better overview of spectral quality and data compared with the traditional way to present LC-MS data. Statistical analysis is carried out on all peptide spots within minutes.

The DeCyder MS algorithm is based on accurate mass and reproducible retention times. Ettan MDLC is ideally suited to deliver reproducible retention times (3), and should be used to generate the data that will be analyzed by DeCyder MS.

In this application note, relative quantitation of allergenic proteins in two
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Comparison of different protein quantitation methods
2. Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
3. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
4. Outstanding transfection rates using the Multiporator
5. Outstanding transfection rates using the Multiporator
6. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
7. Normalizing cDNA libraries using the EppendorfThermomixer
8. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
9. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
10. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
(Date:9/15/2014)... by Northeastern University has developed a novel method ... variety of nanocarbon structures in carbon nanotube arrays. ... will allow the researchers to tailor the physical ... ranging from electronic devices to CNT-reinforced composite materials ... , Their findings were published in the journal ...
(Date:9/15/2014)... , Sept. 15, 2014 The chemistry, taste, ... climate, says a new article published by the nonprofit American ... PhD, on climate change and its effects on the phytochemical ... an extensive study conducted by Dr. Ahmed in the ... and has implications for the future of medicinal ...
(Date:9/15/2014)... -- The second annual International Plasma Awareness Week (IPAW) ... of the Plasma Protein Therapeutics Association (PPTA) ... to: , Raise global awareness about source ... donors in saving and improving lives , Increase ... Member companies are planning a variety ...
(Date:9/13/2014)... Boehringer Laboratories Inc., with a forty ... announce the successful broadcast of a live surgery by ... MD, FACS, FAMBS, and Maher El Chaar, MD, FACS, ... , This sleeve gastrectomy surgery was performed and ... four hundred surgeons from around the world during the ...
Breaking Biology Technology:Northeastern University Researchers Develop Novel Method for Working with Nanotubes 2Tea Taste and Quality Affected by Climate Change 2Tea Taste and Quality Affected by Climate Change 3Tea Taste and Quality Affected by Climate Change 4International Plasma Awareness Week To Celebrate Donors And Spotlight Rare Diseases 2International Plasma Awareness Week To Celebrate Donors And Spotlight Rare Diseases 3Boehringer Laboratories, Inc. Announces Live Surgery Using Innovative System to Calibrate Sleeve Gastrectomy 2
... N.J., May 30, 2012  Today, leading events organizer ... to showcase the latest developments and opportunities across a ... by the same team as InformexUSA, North America,s leading ... chemistry, and managed in conjunction with the UBM team ...
... California Institute for Nursing & Health Care (CINHC) released ... pilot New Graduate Nurse Transition to Practice Programs (RN ... San Francisco, found that the programs are making a ... graduates and increasing their employment opportunities in hospitals and ...
... 2012 Sequenom, Inc. (NASDAQ: SQNM ), a ... the Company,s participation at the Jefferies 2012 Global Healthcare Conference ... 4-7, 2012. Harry F. Hixson, Jr., Ph.D., ... beginning at 11:00 a.m. ET, to provide an overview of ...
Cached Biology Technology:Informex Returns To India In 2012 2Informex Returns To India In 2012 3Study Shows Transition to Practice Programs Get Nurses Employed 2
(Date:9/15/2014)... results will allow us to better understand how ... change scenarios and provide the type of environmental ... management and siting of protected areas," said Paula ... for Coastal Ocean Science (NCCOS) and lead author ... lie within the temperate-tropical transition zone, where historically, ...
(Date:9/15/2014)... Schmid, an assistant professor in the Department of Science ... Arts and Human Sciences at Virginia Tech, has won ... Award to study the prospects and problems of creating ... failure of Japan,s Fukushima I Nuclear Power Plant in ... scientific community viewed nuclear emergencies, Schmid said. Three of ...
(Date:9/15/2014)... lifeplants and animalsto thrive in the oceans, there ... In certain coastal areas, extreme oxygen-starvation produces "dead ... web structure. As dissolved oxygen levels decline, energy ... microbial community metabolism resulting in impacts on the ... the past 50 years, oxygen minimum zones (OMZs) ...
Breaking Biology News(10 mins):Study finds warming Atlantic temperatures could increase range of invasive species 2'Nuclear disasters don't respect national boundaries' 2Decoding virus-host interactions in the oxygen-starved ocean 2Decoding virus-host interactions in the oxygen-starved ocean 3Decoding virus-host interactions in the oxygen-starved ocean 4Decoding virus-host interactions in the oxygen-starved ocean 5
... Ireland Cancer Center of University Hospitals Case Medical Center ... breast cancers express the MUC-1 target. This first-of-its-kind finding, ... has paved the way for an upcoming vaccine trial ... that could potentially prevent recurrence of this aggressive type ...
... from the effects of acid rain, as wet weather counteracts ... The research, by Professor Steve Ormerod and Dr Isabelle Durance ... year period around Llyn Brianne in mid-Wales. Their findings are ... of Applied Ecology . Carried out in 14 streams, ...
... PULLAUP, WA This Christmas season, think twice about ... Christmas tree. The good, old-fashioned methodkeeping your tree in ... to keep your tree green and healthy. Researchers have ... Christmas trees; in fact, in several cases the chemical ...
Cached Biology News:Ireland Cancer Center researcher finds most triple-negative breast cancers express muc-1 target 2Climate-change set-back for acidified rivers 2Flame retardants prove ineffective on fresh-cut Christmas trees 2
... locus encodes two unrelated proteins both of ... the cell cycle in a p53-dependent manner. ... rapid degradation of mdm2 proteinrequired for mdm2 ... This interaction is mediated by the ...
human Exodus-2 Research Focus: cytokine/growth factor Storage Temperature: -20C Shipping Temperature: 4C...
...
Gel Loading Buffer contains a precipitant to ensure that the samples sink into the wells and a tracking dye to allow the user to follow the progress of electrophoresis....
Biology Products: