Navigation Links
Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

Key words: multidimensional liquid chromatography (MDLC) • reversed-phase chromatography (RPC) • LC-MS/MS • DeCyder MS • differential analysis


Ettan™ MDLC was used in conjunction with tandem mass spectrometry (MS/MS) to separate and identify allergenic proteins extracted from the pollen of the olive tree, Olea europaea. DeCyder™ MS Differential Analysis Software (DeCyder MS) was used to quantitate differentially expressed proteins in two different samples, Ole_e1_"mer" and Ole_e1_"lite."


Introduction
Ole e 1 is the most frequent sensitizing agent affecting more than 70% of the patients suffering from olive (Olea europaea) pollinosis. The protein consists of a single polypeptide chain of 145 amino acids (MW 16.3 kDa) and has been suggested to be involved in the hydration of pollen during germination (1).

Ole e 1 exists in several isoforms, displaying microheterogeneities, that are difficult to resolve by gel-based methods. Therefore, we used an LC-MS/MS protein separation and identification strategy.

DeCyder MS Differential Analysis Software (DeCyder MS) is a tool for visualization, detection, comparison, and label-free relative quantitation of LC-MS/MS data (2). In brief, the software displays LC-MS analyses as two-dimensional intensity maps, resulting in a much better overview of spectral quality and data compared with the traditional way to present LC-MS data. Statistical analysis is carried out on all peptide spots within minutes.

The DeCyder MS algorithm is based on accurate mass and reproducible retention times. Ettan MDLC is ideally suited to deliver reproducible retention times (3), and should be used to generate the data that will be analyzed by DeCyder MS.

In this application note, relative quantitation of allergenic proteins in two
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Comparison of different protein quantitation methods
2. Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
3. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
4. Outstanding transfection rates using the Multiporator
5. Outstanding transfection rates using the Multiporator
6. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
7. Normalizing cDNA libraries using the EppendorfThermomixer
8. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
9. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
10. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
(Date:11/26/2014)... 2014 PMG Research, Inc. announced ... is a network of sites that provide clinical research services ... million patient lives through its partnerships with large physician practices ... hub site locations in the Southeastern United States and spans ... the board will be held by: Dr. Robert ...
(Date:11/26/2014)... SonaCare Medical, a leading manufacturer of ... participated in the American Urology Association’s (AUA) surgical ... opinion leaders in urology presented on the latest ... masses while attendees had the opportunity to evaluate ... labs. Attendees at the Los Angeles, CA lab, ...
(Date:11/26/2014)... November 25, 2014 Continuing its award-winning ... earned three prestigious MarCom Awards for outstanding creative achievement ... a Platinum MarCom Award, the organization’s top honor, in ... St. Louis held in April of this year. The ... 14,000 attendees. Additionally, Nerium was awarded a Gold MarCom ...
(Date:11/26/2014)... Global biostimulants market is expected to ... estimated CAGR of 12.5%. In order to cater ... least ecological impacts, farmers are looking to adopt ... yield more effectively & efficiently. Biostimulants are consequently ... return on investments. , Leading companies in the ...
Breaking Biology Technology:PMG Research Names Medical Advisory Board 2PMG Research Names Medical Advisory Board 3SonaCare Medical Supports American Urology Association’s Small Renal Mass 360° Summit 2SonaCare Medical Supports American Urology Association’s Small Renal Mass 360° Summit 3Nerium International™ Wins One Platinum, Two Gold MarCom Awards 22019 Biostimulants Market to Grow at a CAGR of 12.5% Says a New Research Report Available at RnRMarketResearch.com 22019 Biostimulants Market to Grow at a CAGR of 12.5% Says a New Research Report Available at RnRMarketResearch.com 32019 Biostimulants Market to Grow at a CAGR of 12.5% Says a New Research Report Available at RnRMarketResearch.com 4
... the work of the National Nanotechnology Initiative has just ... Technology (NSET) Subcommittee of the National Science and Technology ... Nanotechnology Coordination Office (NNCO). The 2007 NNI ... the NNI to ensure that the United States derives ...
... ... President, ISELIN, N.J., Jan. 3 Pharmos Corporation,(Nasdaq: PARS ) ... Board Changes, Robert F. Johnston has been elected to the ... Ben Dor have retired from,the Board., The Pharmos Board now consists ...
... and Commercial Adoption of the ... Axxent(R) Electronic Brachytherapy System, FREMONT, Calif., Jan. ... System, a proprietary cancer treatment,platform that utilizes X-rays to ... announced that it will,present at the 26th Annual JPMorgan ...
Cached Biology Technology:National Nanotechnology Initiative releases new strategic plan 2National Nanotechnology Initiative releases new strategic plan 3Pharmos Corporation Announces Board and Management Changes 2Pharmos Corporation Announces Board and Management Changes 3Xoft to Present at the 26th Annual JPMorgan Healthcare Conference 2
(Date:11/21/2014)... , November 18, 2014 According ... Market by Systems (Video, RFID, Access Control, Intrusion Detection, ... Hotels, Banks, Government), Component Service Geography - Global Forecasts ... Market is projected to be around $25 Billion in ... 2020, growing at a CAGR of 8.69%. ...
(Date:11/21/2014)... 19, 2014  Earlier this year Donald Spector ... and one of the most prolific inventors in the ... transmitted from Smartphones to third party agencies. Spector envisioned ... one of the earliest known patents in this area. ... the military, child care, elder care and hospital applications. ...
(Date:11/21/2014)... 2014 Strict laws against distracted driving and ... North American and European automotive sector towards gesture recognition ... systems that are intuitive and able to retrieve information ... New analysis from Frost & Sullivan,s ... Europe and North ...
Breaking Biology News(10 mins):Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 2Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 3Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 4
... Cancer and its therapies, including chemotherapy and radiotherapy, ... possibly leading to patient malnutrition, and in severe cases, ... Forest University Comprehensive Cancer Center compilation of various existing ... Journal of Supportive Oncology . One ...
... Swedish medical university Karolinska Institutet (KI) have constructed a mathematical ... increase the understanding of the capacity of the working memory ... interact. One of the findings they have made with this ... that restricts the number of items we can normally store ...
... new study published as the cover article for the April ... ) promises to give physicians new ways to reduce deadly ... cord. In the report, scientists from Columbia University, NY, detail ... cells, called astrocytes, use to handle invading viruses and to ...
Cached Biology News:Taste, odor intervention improves cancer therapy, according to Virginia Tech, Wake Forest study 2Taste, odor intervention improves cancer therapy, according to Virginia Tech, Wake Forest study 3Computer simulations explain the limitations of working memory 2West Nile virus studies show how star-shaped brain cells cope with infection 2
... aptamer screening and binding optimization and built ... on-chip synthesis platform. These microarrays are available ... Microarray Service. Probe Content ... greater than 1500 known aptamer sequences. Add ...
... provides a comprehensive epitope analysis service ... on Paraflo microfluidic chips for immunological ... This comprehensive service includes assistance with ... sample(s) provided by you, single or ...
... Parkers MX80 family of positioners, offering longer travel ... patterns mimic the MX80 family, allowing users to ... to build multi-axis systems., , Miniature linear ... Accelerations to 5 gs , Velocities to 3 ...
... step in the evolution of laboratory automation. ... delivers labware to multiple laboratory instruments, LabLinx ... throughput of ordinary microplate robots. It allows ... configurations to meet any labs specific automation ...
Biology Products: