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DNA Yield with Oragene

ld of Oragene/saliva samples from 208 donors is shown in Figure 2. The median amount of DNA was 110 g. The 25th percentile was 62 g and the 75th percentile was 158 g.


Conclusions
Oragene is a non-invasive DNA self-collection kit that can be used by untrained study subjects, including children and the elderly. The median DNA yield from Oragene/saliva samples is 110 g. This is significantly higher than other oral collection methods.


References
1. Cozier, Y., Palmer, J., and Rosenberg, L. (2003) Comparison of Methods for Collection of DNA Samples by Mail in the Black Womens Health Study. AEP. 14, 117-122.

2. Harty, L., Garcia-Closas, M., Rothman, N., Reid, Y., Tucker, M., and Hartge, P. (2000) Collection of buccal cell DNA using treated cards. Cancer Epidemiology, Biomarkers & Prevention. 9, 501506.

3. Montserrat, G., Egan, K., Abruzzo, J., Newcomb, P., Titus-Ernstoff, L., Franklin, T., et al. (2001) Collection of genomic DNA from adults in epidemiological studies by buccal cytobrush and mouthwash. Cancer Epidemiology, Biomarkers & Prevention. 10, 687-696.

4. Le Marchand, L., Lum-Jones, A., Saltzman, B., Visaya, V., Nomura, A., and Kolonel, L. (2001) Feasibility of collecting buccal cell DNA by mail in a cohort study. 10, 701-703.

5. DNA Genotek Technical Bulletin PR003 Sept 30, DNA Quantifi cation Using the Fluorescence/DNase (F/D) Assay. 2004.


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