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DNA Yield with Oragene

H.C. Birnboim
DNA Genotek, Ottawa, Ontario, Canada

Oragene gives higher DNA yields than other oral collection methods. The median yield of DNA from Oragene samples is 110 g. In comparison, buccal swabs may yield as little as 1.9 g of DNA.


Introduction
The number of studies collecting genomic DNA from a large number of individuals is increasing rapidly. Non-invasive methods and techniques that permit selfcollection are preferred because they increase compliance rates and reduce costs. For this reason, many large-scale studies now use buccal cells from saliva as the source of DNA. The two most common methods are scraping the inside of the cheek with a brush and swishing the mouth with a mouthwash. Oragene is a DNA self-collection kit that is non-invasive and intuitive to use. The donor rinses his or her mouth with water to clear food particles and then spits 2 mL of saliva into the Oragene vial. Once the vial is closed, DNA is released and stabilized at room temperature.

The amount of DNA recovered from the oral cavity can vary widely depending on the collection method (Figure 1). This technical bulletin reports the amount of DNA obtained from saliva using the Oragene kit.


Materials and Methods
Saliva samples were obtained from 208 donors. Collection and purification of DNA was carried out according to protocols supplied with the Oragene kit. DNA yield was determined by the highly specific Fluorescence/DNase method (ref. 5). The F/D method quantitates DNA using SYBR Green I dye (Molecular Probes, Inc.) and DNase treatment.


Results
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Source:


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