Navigation Links
DNA Yield with Oragene

H.C. Birnboim
DNA Genotek, Ottawa, Ontario, Canada

Oragene gives higher DNA yields than other oral collection methods. The median yield of DNA from Oragene samples is 110 g. In comparison, buccal swabs may yield as little as 1.9 g of DNA.


Introduction
The number of studies collecting genomic DNA from a large number of individuals is increasing rapidly. Non-invasive methods and techniques that permit selfcollection are preferred because they increase compliance rates and reduce costs. For this reason, many large-scale studies now use buccal cells from saliva as the source of DNA. The two most common methods are scraping the inside of the cheek with a brush and swishing the mouth with a mouthwash. Oragene is a DNA self-collection kit that is non-invasive and intuitive to use. The donor rinses his or her mouth with water to clear food particles and then spits 2 mL of saliva into the Oragene vial. Once the vial is closed, DNA is released and stabilized at room temperature.

The amount of DNA recovered from the oral cavity can vary widely depending on the collection method (Figure 1). This technical bulletin reports the amount of DNA obtained from saliva using the Oragene kit.


Materials and Methods
Saliva samples were obtained from 208 donors. Collection and purification of DNA was carried out according to protocols supplied with the Oragene kit. DNA yield was determined by the highly specific Fluorescence/DNase method (ref. 5). The F/D method quantitates DNA using SYBR Green I dye (Molecular Probes, Inc.) and DNase treatment.


Results
The DNA yie ld of Oragene/saliva samples from 208 donors is shown in Figure 2. The median amount of DNA was 110 g. The 25th percentile was 62 g and the 75th percentile was 158 g.


Conclusions
Oragene is a non-invasive DNA self-collection kit that can be used by untrained study subjects, including children and the elderly. The median DNA yield from Oragene/saliva samples is 110 g. This is significantly higher than other oral collection methods.


References
1. Cozier, Y., Palmer, J., and Rosenberg, L. (2003) Comparison of Methods for Collection of DNA Samples by Mail in the Black Womens Health Study. AEP. 14, 117-122.

2. Harty, L., Garcia-Closas, M., Rothman, N., Reid, Y., Tucker, M., and Hartge, P. (2000) Collection of buccal cell DNA using treated cards. Cancer Epidemiology, Biomarkers & Prevention. 9, 501506.

3. Montserrat, G., Egan, K., Abruzzo, J., Newcomb, P., Titus-Ernstoff, L., Franklin, T., et al. (2001) Collection of genomic DNA from adults in epidemiological studies by buccal cytobrush and mouthwash. Cancer Epidemiology, Biomarkers & Prevention. 10, 687-696.

4. Le Marchand, L., Lum-Jones, A., Saltzman, B., Visaya, V., Nomura, A., and Kolonel, L. (2001) Feasibility of collecting buccal cell DNA by mail in a cohort study. 10, 701-703.

5. DNA Genotek Technical Bulletin PR003 Sept 30, DNA Quantifi cation Using the Fluorescence/DNase (F/D) Assay. 2004.


back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Yeast Protein Production System Features High Yields and One-Step Purification
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
4. ArrayScript RT: Maximize Yields of Full-length cDNA
5. Increase Protein Activity and Yield
6. High Yield Transcription for Every Application
7. Maximize Viral RNA Yield from Biological Fluid
8. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
9. Synthesize High Yields of Biotinylated aRNA
10. Translate High Yields of Active Protein
11. Translate High Yields of Biologically Active Proteins
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/11/2017)... and LAGUNA HILLS, Calif. , Oct. ... Cancer Research, London (ICR) and University ... SKY92, SkylineDx,s prognostic tool to risk-stratify patients with multiple myeloma ... MUK nine . The University of Leeds ... partly funded by Myeloma UK, and ICR will perform the ...
(Date:10/10/2017)... ... 2017 , ... San Diego-based team building and cooking events company, Lajollacooks4u, has ... The bold new look is part of a transformation to increase awareness, appeal to ... period. , It will also expand its service offering from its signature gourmet cooking ...
(Date:10/10/2017)... 10, 2017 International research firm Parks Associates announced ... at the TMA 2017 Annual Meeting , October 11 in ... residential home security market and how smart safety and security products impact ... Parks Associates: Smart Home ... "The residential security market has ...
(Date:10/9/2017)... DIEGO , Oct. 9, 2017  BioTech ... biological mechanism by which its ProCell stem cell ... critical limb ischemia.  The Company, demonstrated that treatment ... amount of limbs saved as compared to standard ... the molecule HGF resulted in reduction of therapeutic ...
Breaking Biology Technology:
(Date:3/28/2017)... , March 28, 2017 The ... Hardware (Camera, Monitors, Servers, Storage Devices), Software (Video Analytics, ... Region - Global Forecast to 2022", published by MarketsandMarkets, ... 2016 and is projected to reach USD 75.64 Billion ... and 2022. The base year considered for the study ...
(Date:3/24/2017)... 24, 2017 The Controller General of Immigration from ... Abdulla Algeen have received the prestigious international IAIR Award for the ... Continue Reading ... ... Controller Abdulla Algeen (small picture on the right) have received the IAIR ...
(Date:3/23/2017)... The report "Gesture Recognition and Touchless Sensing Market by Technology (Touch-based ... to 2022", published by MarketsandMarkets, the market is expected to be worth USD ... 2022. Continue Reading ... ... ...
Breaking Biology News(10 mins):