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H.C. Birnboim
DNA Genotek, Ottawa, Ontario, Canada
Oragene gives higher DNA yields than other oral
collection methods. The median yield of DNA from
Oragene samples is 110 g. In comparison, buccal
swabs may yield as little as 1.9 g of DNA.
Introduction
The number of studies collecting genomic DNA from a large number of individuals
is increasing rapidly. Non-invasive methods and techniques that permit
selfcollection are preferred because they increase compliance rates and
reduce costs. For this reason, many large-scale studies now use buccal
cells from saliva as the source of DNA. The two most common methods are
scraping the inside of the cheek with a brush and swishing the mouth with
a mouthwash. Oragene is a DNA self-collection kit that is non-invasive
and intuitive to use. The donor rinses his or her mouth with water to
clear food particles and then spits 2 mL of saliva into the Oragene vial.
Once the vial is closed, DNA is released and stabilized at room temperature.
The amount of DNA recovered from the oral cavity can vary widely depending on the collection method (Figure 1). This technical bulletin reports the amount of DNA obtained from saliva using the Oragene kit.
Materials and Methods
Saliva samples were obtained from 208 donors. Collection and purification
of DNA was carried out according to protocols supplied with the Oragene
kit. DNA yield was determined by the highly specific Fluorescence/DNase
method (ref. 5). The F/D method quantitates DNA using SYBR Green I
dye (Molecular Probes, Inc.) and DNase treatment.
Results
The DNA yie
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