Navigation Links
Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

of the sample load used in the assay. Table 2. Comparison of inhibition values obtained from TopCount and LSC.

The results of an 125I-labeled IRMA for TSH counted by liquid scintillation counting on TopCount and on a gamma counter are shown in Figure 3. The standard curves are essentially the same for the two methods of quantitation. Direct counting of immunoassay microplates with TopCount eliminates the need to transfer individual microplate wells to tubes for counting in a gamma counter. The counting efficiency for 125I in TopCount, using MicroScint-O, is higher than 50%.

Conclusions

The Packard TopCount Microplate Scintillation and Luminescence Counter, PicoPlates, and the specially formulated scintillation cocktail, MicroScint- O, provide results from samples in organic solvents or on solid supports equivalent to those obtained with a conventional liquid scintillation counter or gamma counter. Up to 50% sample loads of organic solvents may be used. With small sample loads, counting efficiencies are comparable to conventional LSC. For assays prepared in the microplate, TopCount provides quantitation in the same format. This results in increased throughput, less sample handling, and significant savings in reagent and waste disposal costs.

Acknowledgements

The enzyme inhibition samples were supplied by Dr. Russell Lingham; Merck, Sharpe, and Dohme; Rahway, New Jersey, U.S.A. The TSH immunoassay work was performed by Dr. Alain Baret; Labratoire D'Analysis Medicales; Nantes, France.


'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Counting Cytostar-T scintillating microplates on the MicroBeta JET
3. Counting FlashPlates on the Wallac MicroBeta
4. Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter
5. Direct Counting of Millipore MultiScreen Filtration Plates.
6. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
7. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
10. RNA from LCM Samples
11. Robust High Throughput RNA Isolation from Blood Samples
Post Your Comments:
(Date:10/30/2014)... Isis Pharmaceuticals, Inc. (NASDAQ: ISIS ), the leader ... company overview at the Nomura Biotechnology Conference on Thursday, November ... MA. A live webcast of the ... of the Company,s website, www.isispharm.com .  A replay will ... will be archived for a limited time. About ...
(Date:10/30/2014)... RIDGE, N.J. , Oct. 30, 2014  Regado ... that it will hold a conference call and live ... a.m. EST to discuss its third quarter 2014 financial ... the conference call by dialing (888) 347-1165 for domestic ... call will be webcast live under the investor relations ...
(Date:10/30/2014)... The severe flooding that devastated a wide swath of ... the bridges, roads and other infrastructure had been upgraded ... University of Colorado Denver. , "People need to understand ... PhD, associate professor of structural engineering at the CU ... author the study. "There is an assumption that a ...
(Date:10/27/2014)... The new research report, “Gas Insulated Switchgear ... (Primary & Secondary), and End-User (Transmission & Distribution, ... Generation) - Trends and Forecasts to 2019”, defines ... forecasting of the market size. , Browse more ... spread through 146 Pages and in-depth TOC on ...
Breaking Biology Technology:Isis Pharmaceuticals to Present at the Nomura Biotechnology Conference 2Regado Biosciences to Provide Third Quarter 2014 Financial Results 2CU Denver study says upgrading infrastructure could reduce flood damage 2Gas Insulated Switchgear Market worth $21,951.9 Million by 2019 - New Report by MarketsandMarkets 2Gas Insulated Switchgear Market worth $21,951.9 Million by 2019 - New Report by MarketsandMarkets 3Gas Insulated Switchgear Market worth $21,951.9 Million by 2019 - New Report by MarketsandMarkets 4
... ... attracts investors, COLUMBUS, Ohio, Aug. ... for venture capital investments in biotech innovation and,business growth, according ... markets the state for capital,investment. The report, released by BioEnterprise, ...
... Barr,Pharmaceuticals, Inc. (NYSE: BRL ) today ... a challenge of the patent listed by,Warner Chilcott ... (norethindrone and ethinyl estradiol) chewable oral contraceptive. The,Company ... an Abbreviated New Drug,Application (ANDA) containing a paragraph ...
... Keck Graduate Institute,(KGI) today announced the election of ... M. Burzik, chief executive officer of Kinetic Concepts,Inc.; ... at,Gilead Sciences; and Peter Barton Hutt, senior counsel ... of leaders in business, industry and education who,have ...
Cached Biology Technology:Ohio Ranks Best in the Midwest for Venture Capital Investment in Health Care Bioscience Industry 2Barr Confirms Filing an Application With a Paragraph IV Certification for Femcon(R) FE Chewable Oral Contraceptive Product 2Barr Confirms Filing an Application With a Paragraph IV Certification for Femcon(R) FE Chewable Oral Contraceptive Product 3KGI Elects Three New Members to Board of Trustees 2
(Date:10/30/2014)... help reduce or reverse soil subsidence and reduce ... Sacramento-San Joaquin River Delta by Dartmouth College researchers ... one of the first to continually measure the ... cycle through wetlands, appears in the journal by ... Worldwide, agricultural drainage of organic soils has resulted ...
(Date:10/30/2014)... Philadelphia, PA, October 30, 2014 – Bacteria in ... are critical for digestion. Yet, these same bacteria ... system if they penetrate the gut and enter ... natural response to protect the body, chronic or ... diseases. Prior research has established the involvement of ...
(Date:10/29/2014)... their hair as a consequence of chemotherapy will benefit ... scalp cooling technology that prevents hair loss. , The ... by global scalp cooling manufacturing company, Paxman Coolers, of ... of the University of Huddersfield. , The research will ... a background in the pharmacology of cancer treatment, which ...
Breaking Biology News(10 mins):Dartmouth study finds restoring wetlands can lessen soil sinkage, greenhouse gas emissions 2Breakdown in gut barriers to bacteria may promote inflammation and craving in alcoholics 2New technology on the way to aid cancer suffers who lose their hair after chemotherapy 2
... simple technique to make a common virus-killing material significantly more ... Andrew Barron and Qilin Li. Rather than trying to ... into the public domain. They hope wide adoption will save ... professors and their team reported in Environmental Science and ...
... ITHACA, N.Y. Scientists have identified the genes related ... for planting crops closer together, which has led to an ... Genetics , Jan. 9, 2011.) The study, led ... Agricultural Research Service (USDA-ARS) at Cornell and North Carolina ...
... MD (January 11, 2011)-- The Genetics Society of America ... Research Conference, March 30-April 3, 2011 at the Town ... This annual meeting brings together basic research scientists, ... the common fruit fly, whose hereditary traits Thomas Hunt ...
Cached Biology News:Virus killer gets supercharged 2Virus killer gets supercharged 3Technique allows researchers to identify key maize genes for increased yield 252nd Genetics Society of America Drosophila Research Conference announced 2
Chicken polyclonal to DCTD ( Abpromise for all tested applications). Antigen: Full length protein (Human) Entrez GeneID: 1635 Swiss Protein ID: P32321...
Rab 3 Immunogen: Synthetic peptide corresponding to the N-terminal region of Rab 3 protein. Storage: -20 C, Avoid Freeze/Thaw Cycles...
...
Mouse monoclonal [2G1] to Vancomycin ( Abpromise for all tested applications)....
Biology Products: