Navigation Links
Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

. Dose curves for the enzyme inhibitor and a control sample value obtained with TopCount were compared with those from the Packard Tri-Carb 2250CA.

A sandwich immunoradiometric assay (IRMA) for TSH was performed in a solvent resistant PicoPlate or in microplate strips (Dynex Technologies) coated with anti-TSH MAb as the first antibody. An 125Ilabeled anti-TSH MAb was used to quantitate TSH bound to the solid support by the first antibody. The PicoPlate was counted in the TopCount with 250 μL MicroScint-O, and individual wells of the microplate strip were counted in a Packard Cobra gamma counter for comparison.

Results and Discussion

The average efficiencies and backgrounds obtained with TopCount with a small sample load are presented in Table 1. A small sample (10 μL) was used to provide the optimum cocktail performance. Counting efficiencies for 3H are comparable to those which can be obtained by conventional liquid scintillation counting.

The CPM results for equal volumes of 3H labeled samples in heptane counted with TopCount and a conventional LSC are compared in Figure 1. The data show that the TopCount correlates very well with the LSC (R2= 0.99). The slope of the line indicates that for this sample load TopCount CPM is approximately 40% of the CPM obtained by conventional LSC. This lower efficiency is a result of the much higher sample load; only 250 μL of cocktail was used in TopCount versus 5 mL in the LSC.

Table 2 shows a comparison of the % inhibition values for a control sample obtained from TopCount with the value obtained by LSC. Assays 1-4 were counted with TopCount using four different sample volumes, and assay five was counted with the Tri- Carb 2250CA. These control values are additional confirmation that TopCount provides consistent results regardless
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Counting Cytostar-T scintillating microplates on the MicroBeta JET
3. Counting FlashPlates on the Wallac MicroBeta
4. Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter
5. Direct Counting of Millipore MultiScreen Filtration Plates.
6. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
7. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
10. RNA from LCM Samples
11. Robust High Throughput RNA Isolation from Blood Samples
Post Your Comments:
(Date:3/6/2015)... NC (PRWEB) March 06, 2015 ... innovative specialty CRO focused on specimen management and ... closed. , “Global Specimen Solutions, Inc. is excited ... management with investment that will further enhance our ... first-to-market services supporting specimen research. GSS has proved ...
(Date:3/6/2015)... -- BioSpecifics Technologies Corp. (NASDAQ: BSTC ), ... marketed as XIAFLEX ® (collagenase clostridium histolyticum or ... EU, announced today that it will host a conference ... on Friday, March 13, 2015 to report its fourth ... a corporate update. In order to ...
(Date:3/6/2015)... --  Senomyx , Inc. (NASDAQ: SNMX ), ... discover, develop, and commercialize novel flavor ingredients for the ... Poyhonen , the Company,s President and Chief Executive Officer, ... Chief Financial Officer, will present an overview of Senomyx,s ... at 2:00 p.m. Eastern Time (11:00 a.m. Pacific Time) ...
(Date:3/6/2015)... (PRWEB) March 06, 2015 Transparency Market Research ... and Other Crops - Global Industry Analysis, Size, Share, Growth, ... the global isoxaflutole market was valued at US$ 116.3 Mn ... by 2020, expanding at a CAGR of 5.1% from 2014 ... isoxaflutole stood at 2,569.5 kilo tons in 2013. , Isoxaflutole ...
Breaking Biology Technology:Global Specimen Solutions, Inc. Closes Series A Funding 2Global Specimen Solutions, Inc. Closes Series A Funding 3BioSpecifics Technologies Corp. to Host Conference Call to Report Fourth Quarter and Full Year 2014 Financial Results on Friday, March 13, 2015 2SENOMYX TO WEBCAST CORPORATE PRESENTATION AT THE THE 27TH ANNUAL ROTH CONFERENCE 2Isoxaflutole Market is Expected to Reach US$ 163.0 Mn by 2020 : Transparency Market Research 2Isoxaflutole Market is Expected to Reach US$ 163.0 Mn by 2020 : Transparency Market Research 3
... WALTHAM, Mass., Dec. 11, 2011 Syndax Pharmaceuticals, ... a multicenter, phase 2 study, demonstrated entinostat, a novel, ... showed activity as a single agent in patients with ... being presented today, December 11, from 6:00 to 8:00 ...
... ANGELES, Dec. 9, 2011  Puma Biotechnology, Inc., a ... ongoing Phase II clinical trials of Puma,s investigational ... CTRC-AACR San Antonio Breast Cancer Symposium that is ... presentations are further detailed below: ...
... a heart surgeon at Gundersen Lutheran Health System ... an exciting technique, called minimally invasive coronary surgery ... very small three-inch cut is made between the ribs instead of ... with traditional open heart surgery. This means less pain and ...
Cached Biology Technology:Entinostat Demonstrates Activity in Hodgkin's Lymphoma 2Entinostat Demonstrates Activity in Hodgkin's Lymphoma 3Entinostat Demonstrates Activity in Hodgkin's Lymphoma 4Entinostat Demonstrates Activity in Hodgkin's Lymphoma 5Puma Biotechnology Announces Positive PB272 (Neratinib) Phase II Data at CTRC-AACR San Antonio Breast Cancer Symposium 2Puma Biotechnology Announces Positive PB272 (Neratinib) Phase II Data at CTRC-AACR San Antonio Breast Cancer Symposium 3Puma Biotechnology Announces Positive PB272 (Neratinib) Phase II Data at CTRC-AACR San Antonio Breast Cancer Symposium 4Puma Biotechnology Announces Positive PB272 (Neratinib) Phase II Data at CTRC-AACR San Antonio Breast Cancer Symposium 5Puma Biotechnology Announces Positive PB272 (Neratinib) Phase II Data at CTRC-AACR San Antonio Breast Cancer Symposium 6Gundersen Lutheran Offers Heart Surgery with Less Pain, Faster Recovery 2
(Date:3/5/2015)... , March 5, 2015 In Brazil ... of companies already using these solutions and 42 percent planning ... the latter, approximately 25 percent of the companies will be ... While the majority of companies in the region are currently ... the hybrid model will command significant attention in the coming ...
(Date:3/4/2015)... DUBLIN , Mar. 04, 2015 Research ... announced the addition of the "Global Biometrics ... their offering. The market for biometric ... CAGR of around 14% till 2020 ... will be increasing security needs, government projects and ...
(Date:3/3/2015)... March 3, 2015 Cryoport, Inc. ... advanced cryogenic logistics solutions for the life ... cells, cell lines, clinical research organizations, vaccine ... announced the expansion of its relationship with ... ("Fred Hutch") Clinical Research Division with the ...
Breaking Biology News(10 mins):Two of Every Three Brazilian Companies will have Invested in Cloud by 2015, Finds Frost & Sullivan 2Two of Every Three Brazilian Companies will have Invested in Cloud by 2015, Finds Frost & Sullivan 3Two of Every Three Brazilian Companies will have Invested in Cloud by 2015, Finds Frost & Sullivan 4Global Biometrics Market Forecast and Opportunities, 2020 2Global Biometrics Market Forecast and Opportunities, 2020 3Cryoport, Inc. Expands Support of Fred Hutchinson Cancer Research Center's Clinical Research Division 2Cryoport, Inc. Expands Support of Fred Hutchinson Cancer Research Center's Clinical Research Division 3
... beetles and only two species of the tuatara, a reptile ... Crocodiles and alligators, while nearly 250 million years old, have ... including mammals and many species of birds and fish ... Scientists have often posited that because some ...
... of America (ESA) is pleased to announce the selection of ... Membership acknowledges those who have served ESA for at least ... Society that has reached an extraordinary level. Candidates for this ... voted on by the ESA membership. All four will be ...
... largely restricted to the eastern edge of the New England Tableland, and ... this flowering plant, until now classified as a variety of the Hairpin ... a distinct species. The study was published in the open access journal ... family of flowering plants that includes the Banksia and ...
Cached Biology News:Why are there so many species of beetles and so few crocodiles? 2Why are there so many species of beetles and so few crocodiles? 3Entomological Society of America names 2012 Honorary Members 2Entomological Society of America names 2012 Honorary Members 3Entomological Society of America names 2012 Honorary Members 4Botany student proves 'New England Banksia' a distinct species 2
... MitoPT kit is used in conjunction with your ... and induce apoptosis according to your existing procedure ... a negative control). Once you have induced apoptosis ... to each population and incubate the cells for ...
... The biolistic PDS-1000 He system ... gas to accelerate micron-size nucleic acid-coated ... necessary to transfect cells, tissues, or ... the cell surface. The system includes ...
Sf21 Insect Cells (Frozen) in Max-XP Medium...
... The MyCycler thermal cycler system is ... applications. This system includes the MyCycler personal ... module for use with 0.2 ml tubes ... options, and both algorithm and block modes ...
Biology Products: