Navigation Links
Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

ermined using 3H and 14C hexadecane for both the 96- and 24-well formats. To test the effects of sample load, varying volumes of samples in an organic solvent from an enzyme inhibition assay were counted in 96-well solvent resistant PicoPlates, and the results were compared to those obtained by conventional LSC. A TSH immunoradiometric assay was counted, and the results were compared to those obtained by gamma counting.

Experimental Methods

Counting efficiency was tested by adding 3H hexadecane to three wells of a 96-well PicoPlate containing 250 μL of MicroScint-O. For the 24-well PicoPlate, 3H was added to three wells containing 1.0 mL of cocktail. The same number of wells containing only cocktail were used for background determination. Similar experiments were carried out for 14C. The plates were sealed with Packards TopSealTM-P, a solvent resistant cover film, using the Packard MicroMate 496 Microplate Heat Sealer, and then mixed on a microplate shaker for 15 minutes. The samples were counted for five minutes each, under conditions optimized for liquid counting, on a TopCount with the VariPlateTM option which allows counting of either 96- or 24-well plates. To test the effects of sample load, 3H labeled samples in heptane solvent from an enzyme inhibition assay were counted using four different sample volumes. Aliquots of 25, 50, 100, and 150 μL in quadruplicate were pipetted into a solvent resistant 96-well Pico- Plate with a multiple-tip pipetter. Total volumes were brought to 300 μL with MicroScint-O. The plates were sealed and counted as described above. An additional 50 μL aliquot was counted for five minutes in a Packard Tri-Carb 2250CA liquid scintillation analyzer, using 5 mL Opti-Fluor organic cocktail in 7 mL glass vials, and the results were correlated with those from TopCount
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Counting Cytostar-T scintillating microplates on the MicroBeta JET
3. Counting FlashPlates on the Wallac MicroBeta
4. Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter
5. Direct Counting of Millipore MultiScreen Filtration Plates.
6. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
7. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
10. RNA from LCM Samples
11. Robust High Throughput RNA Isolation from Blood Samples
Post Your Comments:
(Date:7/22/2014)... Albany, New York (PRWEB) July 22, 2014 ... the world. The heat produced by controlled fission chain ... pressure. This steam pressure is used to drive a ... produce approximately 19% of the world’s total electricity production. ... due to several under construction, planned and proposed nuclear ...
(Date:7/22/2014)... Automated cell culture systems are instruments that ... They allow production of optimum cells at a large ... discovery and industries and biopharmaceutical companies. Cell culture automation ... cryobanking, cell-based assays, and cellular expression systems as they ... the Global Cell Culture market will grow ...
(Date:7/22/2014)... 2014 The DNA Spectrum ... visual representation of history, migration, and culture. , ... by family history stories stemming from Europe. His father ... country." Pictures and 8mm home movies painted an elaborate ... family, and Steve ordered a test from DNA Spectrum ...
(Date:7/22/2014)... July 22, 2014 OHAUS Corporation, ... and mechanical balances and scales, proudly announces its ... won Laboratory Equipment magazine’s 2014 “Readers' Choice” Award ... consecutive year the Explorer was voted the best ... Readers’ Choice Awards celebrates “excellence in product design ...
Breaking Biology Technology:Nuclear Energy Market: Global Industry Analysis, Size, Share, Analysis, Growth and Forecast 2013 - 2019 2Nuclear Energy Market: Global Industry Analysis, Size, Share, Analysis, Growth and Forecast 2013 - 2019 3Global Cell Culture Market to Grow at 10.27% CAGR by 2018 Forecasts a New Research Report ReportsnReports.com 2Global Cell Culture Market to Grow at 10.27% CAGR by 2018 Forecasts a New Research Report ReportsnReports.com 3Global Cell Culture Market to Grow at 10.27% CAGR by 2018 Forecasts a New Research Report ReportsnReports.com 4DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 2DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 3DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 4OHAUS Explorer Voted “Best Balance” in Laboratory Equipment’s 2014 Readers’ Choice Awards 2
... , , , JEFFERSON, N.C., ... give people more confidence to go to work in a pandemic ... products the company developed for the bird flu influenza A subtype ... for the bird flu (H5N1), which never became contagious. The Next ...
... , , SEATTLE, Aug. ... Stephen R. Murray, M.D., Ph.D. as Vice President of ... and protocols at Omeros, and currently is leading the ... include OMS103HP, the Company,s lead candidate being evaluated in ...
... , ... pleased to announce that SK Life Science, Inc. (SKLSI), has licensed Pristima for its Research ... ... a world leader in toxicology and pathology data management solutions and the developer of Pristima™ ...
Cached Biology Technology:Next Safety Releases Advanced Respirator for Infectious Diseases 2Next Safety Releases Advanced Respirator for Infectious Diseases 3Omeros Appoints New Vice President of Clinical Development 2SK Life Sciences has Licensed Pristima 2
(Date:7/22/2014)... DURHAM, N.C. In a cost-effectiveness analysis of commercial ... the drug Qsymia showed the best value for the ... loss, but was also the most expensive option tested, ... Graduate Medical School. , The findings, published in the ... important information on the health and weight-loss benefits per ...
(Date:7/22/2014)... NEW YORK, NY -- As part of a multinational, ... at Mount Sinai have helped identify over 100 locations ... developing schizophrenia, in the largest genomic study published on ... The findings, published online in Nature , point ... and could lead to new approaches to treating the ...
(Date:7/22/2014)... have adapted to New York City,s human behaviour, allowing ... than their fellow squirrels in the woods. , Dr ... & Agriculture, led the study that proved eastern grey ... environments and prevent unnecessary responses when humans acted in ... , "As we rapidly increase the spread of ...
Breaking Biology News(10 mins):Study compares cost-effectiveness of weight-loss programs and drugs 2Study compares cost-effectiveness of weight-loss programs and drugs 3Mount Sinai scientists and international team shed new light on schizophrenia 2Mount Sinai scientists and international team shed new light on schizophrenia 3New York squirrels are nuts about city life 2
... natural coal deposits in the Gulf of Alaska are ... Exxon Valdez tanker catastrophe. This clearly disproves the theory ... environmental damage. PAH pollutants were blamed for the continuing ... Then a dispute erupted over the origins of these ...
... Pasadena, Calif.In a finding that sheds new light on ... the California Institute of Technology (Caltech) have pinpointed the ... The discovery, described in the August 30 ... offer insight into autism and other disorders where social ...
... and some amphibians possess a unique sensory capability in ... to "touch" objects in their surroundings without direct physical ... van Hermmen and his team in the physics department ... basis for this sensory system. What they discover might ...
Cached Biology News:Bioavailable contaminants come from the Exxon Valdez oil catastrophe 2Caltech neuroscientists find brain region responsible for our sense of personal space 2Caltech neuroscientists find brain region responsible for our sense of personal space 3Caltech neuroscientists find brain region responsible for our sense of personal space 4Mathematical keys to a sixth sense -- the lateral-line system 2Mathematical keys to a sixth sense -- the lateral-line system 3Mathematical keys to a sixth sense -- the lateral-line system 4
Mouse monoclonal antibody raised against a partial recombinant QPCT. NCBI Entrez Gene ID = QPCT...
... longer need to perform time consuming and inaccurate ... Surveyor, any found variants of the sample when ... in our mutation electropherogram as a sharp peak. ... analysis mode is over 99%, with sensitivity to ...
... low-pressure system, 220-240 V, is used for biolistic ... device employs an adjustable helium pulse to sweep ... the inner wall of a small plastic cartridge ... used for in situ, in vitro, in vivo, ...
The Discovery Series PDQuest 2-D analysis software is used for imaging, analyzing, and databasing two-dimensional electrophoretic gels. The 10-user network license entitles ten users to access data f...
Biology Products: