Navigation Links
Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

Abstract

The microplate format has become a standard for many biological assays such as high throughput screening for drug discovery and radioimmunoassays. Productivity with these high volume procedures has been limited by the amount of sample handling required and low sample counting throughput. Now, however, microplate assays can be efficiently counted in microplates with the Packard TopCount Microplate Scintillation and Luminescence Counter. This paper summarizes TopCounts performance for counting samples in organic solvents or bound to the wells of microplates (in-plate assays) using MicroScintTM-O scintillation cocktail, a special formulation for samples in organic solvents. Results from an enzyme inhibition assay and a microplate immunoassay obtained with TopCount are comparable to those obtained by conventional liquid scintillation and gamma counting.

Introduction

Enzyme inhibition assays are among the many tests used by pharmaceutical companies to screen new compounds for biological activity. These assays measure the inhibitory effect of test compounds on target enzymes such as HIV proteases, various kinases, RNA and DNA transcriptases and polymerases. Enzyme activity is often determined by measuring the conversion of a radiolabeled substrate to a product that can be separated from the substrate by chromatography or organic extraction. After the separation step, the 3H- or 14C-labeled products, often in organic solvents, are counted by liquid scintillation counting (LSC). The assays are often done in microplates, and it is highly desirable to quantitate the radiolabeled product in this format, especially when the product can be eluted directly into microplates. By quantitating in microplates, additional sample handling and liquid scintillation vials can be eliminated. In addition, sample and cocktail volumes, and the resulting waste d
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Counting Cytostar-T scintillating microplates on the MicroBeta JET
3. Counting FlashPlates on the Wallac MicroBeta
4. Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter
5. Direct Counting of Millipore MultiScreen Filtration Plates.
6. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
7. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
10. RNA from LCM Samples
11. Robust High Throughput RNA Isolation from Blood Samples
Post Your Comments:
(Date:9/2/2014)... Sept. 2, 2014 Orexigen Therapeutics, Inc. (Nasdaq: ... Patent and Trademark Office (PTO) has issued a patent ... for weight loss. NB32 is a fixed-dose combination of ... U.S. Patent No. 8,815,889 claims methods for treating insulin ... patent expires in 2024. If NB32 is approved for ...
(Date:9/2/2014)... SEATTLE , Sept. 2, 2014 /PRNewswire/ - Oncothyreon Inc. (Nasdaq: ... , M.D., President and Chief Executive Officer, will present at ... Conference in New York on Tuesday, ... A live and archived webcast of the presentation will be ... the "News & Events" section. About Oncothyreon ...
(Date:9/2/2014)... Sept. 2, 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or ... The orders consist of three large odour control systems for ... "These orders are consistent with the market resurgence ... Bruijns , President and CEO. "After a difficult period of ... now beginning to see sales growth. Our lower cost structure, ...
(Date:9/2/2014)... 2, 2014  Spherix Incorporated (SPEX) -- an intellectual property development ... property, today announced that the United States Patent & ... in the month of August that are part of ... The issued patents are: , U.S.RE45,065 ... 19, 2014; and , U.S.RE45,095 issued August 26, ...
Breaking Biology Technology:Orexigen Expands NB32 Intellectual Property Portfolio With New U.S. Patent 2Orexigen Expands NB32 Intellectual Property Portfolio With New U.S. Patent 3Orexigen Expands NB32 Intellectual Property Portfolio With New U.S. Patent 4United States Patent & Trademark Office Issues Three New Standard Essential Patents to Spherix 2
... Third Wave Technologies is appointing more new ... to a press release. , ,Maneesh Arora, 37, will become ... has held senior positions in marketing and operations for the ... 35, will be vice president of finance. , ,John Bellano, ...
... investments in 2005 added up to more than $19 million ... report released on Wednesday. , ,The investor tax credits offered ... start to officially track the volume of investments by individuals. ... picture we've had of what's going on in the individual ...
... of financing is vital to a biotech company (it is ... "biotech" in the broadest sense) companies are continually faced with ... development cycle, this week's article will focus on venture capital ... on VC financing results from Burrill and Company's data. Burrill's ...
Cached Biology Technology:State tracks more angel investment, at least $19M last year 2U.S. VC funding flat in 2005 while life science funding grows 2U.S. VC funding flat in 2005 while life science funding grows 3U.S. VC funding flat in 2005 while life science funding grows 4
(Date:9/1/2014)... in the U.S. undergoes a battery of tests designed ... disease. Thousands of children born in the developing world, ... from the disease each year. , A.J. Kumar ... of those deaths. , A Post-Doctoral Fellow in ... George Whitesides, the Woodford L. and Ann A. Flowers ...
(Date:9/1/2014)... nucleotidethe basic building block of DNAcould initiate fragile X syndrome, the ... The Journal of Cell Biology . ... gene on the X chromosome called fragile X mental retardation ... 360 men carry a so-called premutation, in which a series of ... is slightly longer than normal. These repeats are prone to even ...
(Date:9/1/2014)... As nocturnal animals, bats are perfectly adapted to a life ... between the reflected echoes to measure distance to obstacles or ... different echo delays. A study carried out by researchers at ... that this map dynamically adapts to external factors. , ... bat flies in too close to an object, the number ...
Breaking Biology News(10 mins):Faster, cheaper tests for sickle cell 2Faster, cheaper tests for sickle cell 3A nucleotide change could initiate fragile X syndrome 2Zooming in for a safe flight 2
... are labeled a “night owl?report more pathological symptoms related ... for their nocturnal sleeplessness by extending their time in ... time, according to a study published in the April ... (JCSM). , The study, authored by Jason C. Ong, ...
... Southwestern Medical Center have found a new method for ... the most common acute infections in children. , ... by tracking down the virus or bacteria causing it. ... virus may not be present in the blood or ...
... Institutes of Health have discovered how a defect in ... several genes interact to create myelin, a fatty coating ... reliability of their electrical signals. , The discovery ... These disorders can affect the peripheral nervous system—the nerves ...
Cached Biology News:'Night owls' report more insomnia-related symptoms 2Infection detectives use disease 'fingerprints' to track common infections in children 2Infection detectives use disease 'fingerprints' to track common infections in children 3Researchers discover gene crucial for nerve cell insulation 2Researchers discover gene crucial for nerve cell insulation 3
Request Info...
FITC~Bovine Serum Albumin...
, Premium quality synthesis and purification , You provide the sequence, we'll do the rest , Rigorous quality control , Your choice of purification method, amount and format , Available annealed...
... siRNA service provides the premium quality siRNAs ... Custom siRNAs can be readily ordered online ... sequence, or by providing the sense and ... Silencer Validated and Silencer ...
Biology Products: