Quantitating cell number is more accurate using the Quantos cell proliferation assay kit
Leslie S. Arrington
Genetic Applications LLC
The Quantos cell proliferation assay kit provides a simple, reproducible method to assess the number of cells present in various tissue culture dish formats. In this study, we compared how well the kit performed compared to another commercial fluorescence-based cell proliferation assay product. The data demonstrated that the kit more accurately assessed cell number in the range of cell number frequently found in commonly used culture dish formats.
Researchers are frequently interested in determining the effects of various drugs, toxins, or experimental conditions on cell proliferation. With Stratagenes new Quantos cell proliferation assay kit, it is easy to detect cell number directly in the tissue culture well: After aspirating cell culture media, add the Quantos dye reagent, and measure fluorescence in each well using a fluorometer. Since the Quantos dye fluoresces only when bound to DNA, the assay is insensitive to changes in cellular RNA concentrations or proteins. Relative fluorescence units from the sample wells are compared to a standard curve prepared beforehand. In this way, the number of cells in each well of a tissue culture plate can be quickly and accurately determined.
We set out to determine how well the Quantos cell proliferation assay kit compares to another widely available cell proliferation assay product that uses a proprietary green fluorescent dye. Standard curves were made using adherent and suspension cells at various cell densities ranging from 0 to 80,000 cells/well in a 96-well format. The results were plotted, and trendlines for each assay were calculated.