Navigation Links
C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR

Polymerase for two-step RT-PCR amplification of up to 4 kb targets

Cat. No. 2 016 311 300 units

Description C. therm. Polymerase provided by Roche Applied Science is the recombinant form of the DNA polymerase from the thermophilic eubacterium Carboxydothermus hydrogenoformans, expressed in E. coli. The recombinant enzyme lacks the 5'3' exonuclease domain (RNase H activity) of the native enzyme. Application Produces first strand cDNA (for RT-PCR) or full-length double-stranded cDNA transcripts from total RNA or mRNA. May be used for:
  • Two-step RT-PCR for virus detection, cloning, mutation analysis, etc.
  • One-step RT-PCR (e.g. when used with Taq DNA Polymerase).
  • One-Step RT-PCR System.
  • RT-PCR amplification of GC-rich sequences.
Operating parameters
  • Temperature optimum, reverse transcriptase reaction: 5572C.
  • Divalent ion requirement: Mg2+.
  • Addition of betaine (0.52.0 M) to reaction mix improves yield and specificity of transcription for GC-rich templates.
  • Accepts DIG-labeled and other modified nucleotides.
  • Works with Uracil-DNA Glycosylase (PCR carry-over prevention).
Key advantages
  • Enhances specificity, because the high reaction temperature reduces nonspecific primer binding.
  • Ensures high fidelity first strand cDNA, because the enzyme has a twofold lower transcriptional error rate than Tth DNA Polymerase.
  • Efficiently transcribes GC-rich mRN
    '"/>


Source:


Page: All 1 2

Related biology technology :

1. C. therm. Polymerase One-Step RT-PCR System
2. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
3. Pfu DNA Polymerase as a Hot Start Alternative
4. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
5. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
6. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. High-Fidelity PCR with a Novel Polymerase Mixture
9. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
10. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
11. The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: therm Polymerase for Reverse Transcription Two Step PCR

(Date:10/22/2014)... Shimadzu Scientific Instruments introduces its Open Solution QuantAnalytics(TM) ... management and clinical markets. The software increases the speed ... users to highlight and review results that are of ... to filter results by group or based on a ... need to sift through analytes of no interest. In ...
(Date:10/22/2014)... 22, 2014 Physicians Choice Laboratory ... pharmacogenetics menu, which enables healthcare providers to individualize ... PCLS’s evidence-based results, healthcare providers are better able ... therapy, while minimizing risks for adverse drug reactions ... to the FDA [1] more than 2.2 million ...
(Date:10/22/2014)... Research and Markets  has announced the addition of ... Geography - Global Analysis and Forecast (2014 - 2020)" ... electronics, also called as polymer electronics or plastic electronics, ... small conductive molecules and electrically conductive polymers. It is ... carbon based, made using synthetic strategies developed in the ...
(Date:10/20/2014)... PARK, Calif. , Oct. 20, 2014 /PRNewswire/ ... today that the Company has signed a Notice ... of Regenerative Medicine (CIRM), effective October 1, 2014.  ... Clinical Development payments and the release of additional ... million CIRM grant award for clinical development of ...
Breaking Biology Technology:Shimadzu Releases QuantAnalytics Open Access LCMS Software Package for Clinical Applications 2PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 2PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 3Global Organic Electronics (Semiconductor, Conductive, Dielectric and Substrate) Market - Analysis and Forecast (2014 - 2020) 2Global Organic Electronics (Semiconductor, Conductive, Dielectric and Substrate) Market - Analysis and Forecast (2014 - 2020) 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 2Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 4
... SOUTH SAN FRANCISCO, Calif., May 15 Poniard Pharmaceuticals, ... focused on oncology, today announced that it has filed ... Securities and Exchange Commission (SEC) that, when declared effective ... time to offer and sell up to $60 million ...
... JOLLA, Calif., May 15 Fate Therapeutics, ... exclusive intellectual property rights jointly owned by Children,s Hospital ... covers compositions and methods for supporting hematopoietic stem cells. ... director of the Stem Cell Program at Children,s Hospital ...
... Shandong, China, May 15 /PRNewswire-Asia-FirstCall/ -- China,Biologic Products, ... the,Company,), one of the leading plasma-based pharmaceutical companies ... that the Company,s,management will present at the upcoming ... New York, NY on May 18, 2009 and ...
Cached Biology Technology:Poniard Pharmaceuticals Files Shelf Registration 2Poniard Pharmaceuticals Files Shelf Registration 3Fate Therapeutics Granted Exclusive License for Stem Cell Modulators by Children's Hospital Boston and Massachusetts General Hospital 2Fate Therapeutics Granted Exclusive License for Stem Cell Modulators by Children's Hospital Boston and Massachusetts General Hospital 3China Biologic Products to Present at CCG IR's China Rising Conference and Oppenheimer's China Dragon Call Conference in New York 2China Biologic Products to Present at CCG IR's China Rising Conference and Oppenheimer's China Dragon Call Conference in New York 3China Biologic Products to Present at CCG IR's China Rising Conference and Oppenheimer's China Dragon Call Conference in New York 4
(Date:10/17/2014)... critical and pertinent for practicing physicians and clinicians given ... The Journal, Disaster Medicine and Public Health Preparedness ... Public Health, to surround the public, medical professionals and ... , On October 17, the journal published ... was prepared by Dr. Eric Toner, internist and emergency ...
(Date:10/17/2014)... from the University of Copenhagen have shown for the ... lungs of Cystic fibrosis patients, giving them the opportunity ... in chronic infections. , The study also discovered the ... (CF) patients was halted or slowed down by the ... all the oxygen and helped "suffocate" the bacteria, forcing ...
(Date:10/16/2014)... the human body. Battles are won, lost or ... this stalemate—known as tumor dormancy—can last up to ... that is poorly understood. , A new ... Torquato, a Professor of Chemistry at Princeton University, ... and the switch to a malignant state. Published ...
Breaking Biology News(10 mins):Scientists opens black box on bacterial growth in cystic fibrosis lung infection 2Modeling tumor dormancy 2
... cardiovascular disorders and old age have one thing in ... scientists have observed oxidative changes in important biomolecules. These ... free radicals that are formed as a by-product of ... acids. Today, reactive oxygen molecules are no longer ...
... CAMBRIDGE, Mass.--As Charles Darwin showed nearly 150 years ago, bird ... team of MIT mathematicians and engineers has now explained exactly ... gravity and transport food into their mouths. The phalarope, ... interactions between its beak and water droplets to propel bits ...
... Contrary to long-held assumptions, high-salt diets may ... investigators from the Albert Einstein College of Medicine ... examining dietary intake among a nationally representative sample ... actually observed a significantly increased risk of death ...
Cached Biology News:Biosensor for measuring stress in cells 2MIT solves gravity-defying bird beak mystery 2MIT solves gravity-defying bird beak mystery 3New study casts further doubt on risk of death from higher salt intake 2
DTT, 25g...
... Agarose overlays are commonly used ... commercial "DNA grade" agaroses either ... to insect cells or lack ... obtain good overlays. Novagen's BacPlaque ...
Antibody specific for trimethyl-Lys27 of histone H3. Mouse monoclonal. Antigen: Synthetic peptide derived from histone H3, trimethylated at lysine 27...
...
Biology Products: