Navigation Links
ArrayScript RT: Maximize Yields of Full-length cDNA

al RNA, ArrayScript often eliminates the need for two rounds of amplification, still generating sufficient cRNA for array analysis. This translates to significant savings of time and reagents.

Figure 2. ArrayScript Reverse Transcriptase Produces up to Twice the cRNA Yield in MessageAmp II. A comparison of ArrayScript RT (AS; 200 U), SuperScript II (SSII; 200 U); wild type M-MLV RT (200 U), and wild type AMV RT (10 U) in MessageAmp II conditions demonstrates that ArrayScript outperforms other commercial RTs. 90 ng HeLa-S3 total RNA (28S/18S ratio = 1.7) was heat denatured in the presence of T7 Oligo(dT) at 70C for 10 minutes. Reverse transcription was initiated with the RT enzyme and incubated at 42C for 2 hr in an air incubator. Following first-strand cDNA synthesis, DNA polymerase was added for second-strand synthesis, which was incubated at 16C for 2 hr in a thermocycler (heated lid option turned off). The double-stranded cDNA was then filter purified and added to a 4 hr in vitro transcription reaction. Last, the resulting cRNA was purified by glass filter and eluted in nuclease-free water for analysis by Agilent 2100 bioanalyzer and NanoDrop Spectrophotometer.


ArrayScript is Suitable for all RT Applications
Reverse transcriptases are also used in RT-PCR, cDNA library construction, and RLM-RACE. In most of these applications, synthesis of full-length cDNA is a critical parameter that affects final experimental results. For example, high yields of full-length cDNA result in the generation of cDNA libraries that more faithfully represent the cellular transcriptome. The
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Maximize Viral RNA Yield from Biological Fluid
2. Rapid Chromatography Optimization by pH Scouting Using the New BioLogic Maximizer Valve System, Rev A
3. Yeast Protein Production System Features High Yields and One-Step Purification
4. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
5. Synthesize High Yields of Biotinylated aRNA
6. Translate High Yields of Active Protein
7. Translate High Yields of Biologically Active Proteins
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: ArrayScript Maximize Yields Full length cDNA

(Date:9/3/2015)... ... September 03, 2015 , ... Michigan-based technology startup ... early 2016. , The device, called the Halo H1, is a wearable ... sensing technology. These sensors track trending interstitial fluid levels in the body, in ...
(Date:9/2/2015)... 2015 Axovant Sciences Ltd. (NYSE:  AXON), a leading ... announced upcoming presentations at three investor conferences: , ... Healthcare Conference in New York at ... at 2:00 PM at the BioCentury NewsMakers Conference in ... & Conference Center , Thursday, September 17 at 9:30 ...
(Date:9/2/2015)... ... September 02, 2015 , ... ... compliance, quality systems, analytical testing, education and consulting services for the medical ... medical device products/processes development, design control, validation, quality assurance and regulatory compliance. ...
(Date:9/2/2015)... Research and Markets ... Jain PharmaBiotech,s new report "Biochips and Microarrays ... offering. This report is an analysis ... The report starts with a description of technologies ... include array comparative genomic hybridization (CGH), copy number ...
Breaking Biology Technology:Technology Startup Halo Wearables to Unveil Non-Invasive Hydration Monitoring Device Early 2016 2Axovant Sciences To Present At Upcoming Investor Conferences 2Axovant Sciences To Present At Upcoming Investor Conferences 3NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 2NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 3NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 4NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 5NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 6NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 7Global Biochips and Microarrays Technologies, Markets and Companies Report 2015 with Market Size in 2014 and Projected Value for the Years 2019 and 2024 2
... company adds new apoAI measurement and apoB/apoAI ratio to ... March 17 /PRNewswire/ -- Atherotech, ... Cholesterol Test in booth #1263 at ACC.09, the American ... The annual meeting takes place March 29-31, 2009, at ...
... Mass., March 17 The pro bono healthcare ... ), has announced that its newly instituted Safe ... Lutheran Health System of La Crosse, Wisconsin. The ... outstanding accomplishments in safe patient handling, safe lifting, ...
... March 17 Cardiorobotics, Inc., a ... use in a wide range of applications, including: ... appointment of Dr. Samuel Straface in the position ... that Dr. Straface has joined Cardiorobotics in this ...
Cached Biology Technology:Atherotech to Exhibit the VAP(R) Cholesterol Test at ACC.09 in Orlando 2Atherotech to Exhibit the VAP(R) Cholesterol Test at ACC.09 in Orlando 3Gundersen Lutheran Health System is Recognized for Safe Lifting Leadership 2Gundersen Lutheran Health System is Recognized for Safe Lifting Leadership 3Cardiorobotics Announces New CEO, PLSG Executive-in-Residence to Lead Company 2Cardiorobotics Announces New CEO, PLSG Executive-in-Residence to Lead Company 3
(Date:8/12/2015)... , Aug. 12, 2015  New research ... can attack Android phones to steal users, fingerprints. ... increasing availability of fingerprint scanners on mobile devices, ... concern. To secure biometrics on mobile devices, HYPR ... augment these convenient authentication systems with strong cryptographic ...
(Date:8/6/2015)... CITY, Calif. , Aug. 6, 2015 /PRNewswire/ ... Valley) will visit Crossmatch ™ to learn ... networks and protect data. Crossmatch is a world ... whose technologies and expertise are helping federal agencies ... efficiency and security.   "The vast ...
(Date:8/5/2015)... VIEW, Calif. , Aug. 5, 2015 ... exhibits continuous growth in applications, penetration into newer sectors, ... after year. The global biosensors space has seen the ... exited the market so far. (Photo - ... Frost & Sullivan, Analysis of the Global Biosensors ...
Breaking Biology News(10 mins):HYPR Corp. Addresses Android Vulnerability That Exposes Ecosystem-wide Biometric Security Challenges 2HYPR Corp. Addresses Android Vulnerability That Exposes Ecosystem-wide Biometric Security Challenges 3Rep. Honda to Visit Crossmatch in Redwood City 2Innovative Biosensors Incite Use in Non-Traditional Applications 2Innovative Biosensors Incite Use in Non-Traditional Applications 3Innovative Biosensors Incite Use in Non-Traditional Applications 4
... A study led by researchers from Universitat Autnoma de Barcelona ... populations control the way they spread over the surface of ... presence of an antibiotic, only to resume again when the ... RecA protein, which significantly increases its concentration at the start ...
... in San Diego, picked up a copy of the Los Angeles ... on the wildfires in the western United States. The wildfires, ... years instead of every few decades and they are burning ... cheatgrass (Bromus tectorum), an invasive plant that is rapidly displacing native ...
... 2010 -- With the signing of a memorandum of ... 20 planned core ecological observatory sites that will provide ... ecosystem breathes. The agreement between the Department of ... Walker Branch Watershed portion of the $434 million project ...
Cached Biology News:Discovery of controlled swarm in bacteria 2In elevated carbon dioxide, soybeans stumble but cheatgrass keeps on truckin' 2In elevated carbon dioxide, soybeans stumble but cheatgrass keeps on truckin' 3In elevated carbon dioxide, soybeans stumble but cheatgrass keeps on truckin' 4In elevated carbon dioxide, soybeans stumble but cheatgrass keeps on truckin' 5DOE, ORNL officially join NSF on massive ecological study 2