Navigation Links
ArrayScript RT: Maximize Yields of Full-length cDNA


Get Full-length cDNA
Ambion scientists have engineered a mutant form of M-MLV reverse transcriptase to improve production of full-length cDNA. This engineered reverse transcriptase, ArrayScript (patent pending), produces significantly higher yields of full-length cDNA than wild type RTs, and rivals even the newest engineered RTs on the market (Figure 1). Efficient synthesis of full-length cDNA is important for microarray analysis; it results in low 3'/5' ratios, which are a hallmark of high quality microarray data.

Figure 1. ArrayScript Reverse Transcriptase Synthesizes Intact cDNA Products as Long as 9 kb. cDNA synthesis was performed with 100 U of either ArrayScript RT, SuperScript II (SSII), SuperScript III (SSIII), or wild type (wt) M-MLV RT in a 20 l reaction volume containing a synthetic, poly(A)-tailed 9 kb transcript (1 g), 1X RT buffer, 0.5 M oligo(dT) primer, 0.5 mM dNTPs, 40 U RNase Inhibitor, and 0.1 l [a-32P]dATP. Reactions were incubated for 1 hr at 42C and quenched with EDTA. The cDNA products were resolved on a 1% denaturing agarose gel and detected with a phosphorimager.


Double the Yield in RNA Amplification
One of the most rigorous applications for an RT enzyme is RNA amplification from low amounts of total RNA. In this application, first-strand cDNA generated with ArrayScript resulted in up to twice the yield of amplified RNA compared to cDNA synthesized using wild type M-MLV, AMV, or other engineered M-MLV reverse transcriptases (Figure 2). When starting with low amounts of tot
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Maximize Viral RNA Yield from Biological Fluid
2. Rapid Chromatography Optimization by pH Scouting Using the New BioLogic Maximizer Valve System, Rev A
3. Yeast Protein Production System Features High Yields and One-Step Purification
4. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
5. Synthesize High Yields of Biotinylated aRNA
6. Translate High Yields of Active Protein
7. Translate High Yields of Biologically Active Proteins
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: ArrayScript Maximize Yields Full length cDNA

(Date:10/19/2014)... The report "Unmanned Ground Vehicle ... Technology, Payload, Type & by Geography - Forecasts ... unmanned ground vehicle market into various sub segments ... sizes. The report also identifies the factors driving ... along with the adoption trends. , The unmanned ...
(Date:10/18/2014)... 18, 2014 The Asian arthroscopic devices ... with analysis and forecast of revenue. The arthroscopic devices ... million by 2018, at a CAGR of 6.5% from ... the Asian arthroscopic devices market, to get an idea ... glimpse of the segmentation of arthroscopic devices market in ...
(Date:10/18/2014)... According to a new market research report “Hydraulic ... Trends & Forecasts up till 2017”, hydraulic fracturing market ... value is expected to increase from $40 billion in ... during the same period. North America is expected to ... of hydraulic horse power supplied by the year 2011. ...
(Date:10/17/2014)... and HILDEN, Germany , ... , Translational research in cancer and ... presented at the American Society of Human Genetics ... DNA samples linked to Ingenuity ®  Knowledge Base ... genomic analysis  Ingenuity ®  Variant Analysis™ ...
Breaking Biology Technology:Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 2Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 3Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 4Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 5The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 2The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 3The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 4Hydraulic Fracturing Market Projected To Reach $64 Billion by 2017 - New Report by MarketsandMarkets 2Hydraulic Fracturing Market Projected To Reach $64 Billion by 2017 - New Report by MarketsandMarkets 3Hydraulic Fracturing Market Projected To Reach $64 Billion by 2017 - New Report by MarketsandMarkets 4QIAGEN Demonstrates Expanded Bioinformatics Workflows at ASHG; Strong Market Adoption as Researchers Analyze Genomic Data on More Than a Quarter-Million Samples in Secure Private Cloud 2QIAGEN Demonstrates Expanded Bioinformatics Workflows at ASHG; Strong Market Adoption as Researchers Analyze Genomic Data on More Than a Quarter-Million Samples in Secure Private Cloud 3QIAGEN Demonstrates Expanded Bioinformatics Workflows at ASHG; Strong Market Adoption as Researchers Analyze Genomic Data on More Than a Quarter-Million Samples in Secure Private Cloud 4QIAGEN Demonstrates Expanded Bioinformatics Workflows at ASHG; Strong Market Adoption as Researchers Analyze Genomic Data on More Than a Quarter-Million Samples in Secure Private Cloud 5QIAGEN Demonstrates Expanded Bioinformatics Workflows at ASHG; Strong Market Adoption as Researchers Analyze Genomic Data on More Than a Quarter-Million Samples in Secure Private Cloud 6
... Pharmaceutical Gains Rights to Develop and Market Nitazoxanide ... Feb. 17 Romark Laboratories, a privately held ... member of the Roche Group, today announced that ... license agreement for the development and commercialization of ...
... on Therapy Development StrategyCAMBRIDGE, Mass., Feb. 17 ... announced the 2009 launch of its popular ALS ... is designed to inform and update patients, their ... discover and develop effective treatments for amyotrophic lateral ...
... Weaken in Many CountriesNEW YORK, Feb. 17 New TNS ... five major European countries -- the UK, Germany, Spain and ... sales and service activities. Merck takes the top spot ... and Germany. In 2008, Merck and Novartis tied for ...
Cached Biology Technology:Romark and Chugai Pharmaceutical Enter Into Exclusive Licensing Agreement for Nitazoxanide in Japan 2Romark and Chugai Pharmaceutical Enter Into Exclusive Licensing Agreement for Nitazoxanide in Japan 3Seminar Will Bring ALS Researchers to Patient Groups 2Seminar Will Bring ALS Researchers to Patient Groups 3Physicians in Europe Rate Novartis #1 on Service Delivery, While Merck Earns Top Honors in the US 2Physicians in Europe Rate Novartis #1 on Service Delivery, While Merck Earns Top Honors in the US 3Physicians in Europe Rate Novartis #1 on Service Delivery, While Merck Earns Top Honors in the US 4Physicians in Europe Rate Novartis #1 on Service Delivery, While Merck Earns Top Honors in the US 5Physicians in Europe Rate Novartis #1 on Service Delivery, While Merck Earns Top Honors in the US 6Physicians in Europe Rate Novartis #1 on Service Delivery, While Merck Earns Top Honors in the US 7
(Date:10/16/2014)... Institute of Marine Science have received a 3-year, ... identify the streams and wetlands most vulnerable to ... local governments and citizens conserve these important ecosystems. ... of tidal-marsh observations first gathered by VIMS scientists ... can today,s researchers accurately map the slow but ...
(Date:10/16/2014)... Melbourne researchers have challenged conventional thinking on how the ... new mechanism for how bowel cancer starts. , The ... maintaining and regenerating the ,crypts, that are a feature ... are involved in bowel cancer development, a controversial finding ... existence. , Using 3D imaging technologies, Dr Chin Wee ...
(Date:10/16/2014)... Respiratory Syncytial Virus causes severe respiratory tract infections ... each year. Scientists at VIB and Ghent University ... to counteract this common virus infection. , ... strategy that paves the way for the development ... a virus that causes suffering in numerous small ...
Breaking Biology News(10 mins):EPA grant will help localities conserve headwater wetlands 2EPA grant will help localities conserve headwater wetlands 3Cryptic clues drive new theory of bowel cancer development 2New perspectives for development of an RSV vaccine 2
... Laura Burkle and her colleagues captured 2,778 bees while retracing ... between bees and flowering plants more than a century ago. ... to the rich historic records that guided her field work, ... published their results in " Science ." "It,s exciting," ...
... Ecologists have evidence that some endangered primates and large ... in the sultry thickets of mangrove and peat swamp ... vegetation particularly clusters of salt-loving mangrove trees ... peat, which is the waterlogged remains of partially decomposed ...
... Coen from the John Innes Centre has been awarded 2.5M ... plants. "Carnivorous plants turn the normal order of ... them," said Karen Lee, a researcher working on the project ... a fascinating example of how new shapes and structures evolve ...
Cached Biology News:Old records, new bees result in 'Science' paper for MSU ecologist 2Old records, new bees result in 'Science' paper for MSU ecologist 3Where the wild things go… when there's nowhere else 2Exploring the inner world of carnivorous plants 2
A set of five proteins which can be used to calibrate a Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) or Electrospray Ionization (ESI) mass spectrometer....
... of Grace's medium were developed in several ... Many of these modifications were developed by ... cells derived from the cabbage looper,Tricoplusia ni. ... TNM-FH. The medium when properly supplemented has ...
... The LIC Duet™ GST•Tag™ Ek Adaptor ... operator, ribosome binding site (rbs), ATG translation ... sequence. The GST•Tag sequence has been reported ... cases the solubility of its fusion partners. ...
...
Biology Products: