Navigation Links
Are siRNA Pools Smart?

omplement of interesting genes within the collection being analyzed (Figure 5).


Phenotype vs. siRNA Efficacy

One possible explanation for the discrepancy between single siRNAs and pooled siRNAs is that the levels of target gene expression following transfection were different in each case. To address this, we measured cell number and mRNA knockdown in cells transfected with single and pooled siRNAs targeting nine different genes. As shown in Figure 6, the average correlation between cellular phenotype and mRNA knockdown for each of the different genes was low, indicating that the variability in phenotypes induced by different siRNAs was unlikely to have been caused by siRNA efficacy.


[click here to enlarge]

Figure 6. Phenotype vs. mRNA Knockdown in Transfected Cells. HeLa cells were transfected with three different single siRNAs as well as an siRNA pool targeting each of the nine genes shown on the x-axis. Three days post-transfection, the wells were assayed for cell number (A) using alamarBlue (AccuMed International). The RNA in the cells was isolated and assayed for target mRNA expression by real-time RT-PCR (B). The alamarBlue and the target mRNA quantification are presented as percentages of cells transfected with with Silencer Negative Control #1 siRNA (Ambion).


Conclusions

Similar comparisons of single siRNAs versus pooled siRNAs were carried out using nine different phenotypic assays including protein activation, cell mor
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Custom and library siRNA for efficient gene silencing
2. Custom and library siRNA for efficient gene silencing
3. Cancer siRNA Oligo Set Version 1.0
4. Library siRNA
5. Custom siRNA Oligo Synthesis Service
6. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
7. Quantification of siRNA Silencing Efficiency Using the LightCycler System
8. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
9. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
10. siRNA transfection optimization with the Agilent 2100 bioanalyzer
11. siRNA Design Guidelines
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Are siRNA Pools Smart

(Date:5/28/2015)... , May 27, 2015 Research and ... Jain PharmaBiotech,s new report "Cell Therapy - ... This report describes and evaluates cell ... to play an important role in the practice ... the old fashioned bone marrow transplants. Role of ...
(Date:5/28/2015)... , May 27, 2015 Research and ... Jain PharmaBiotech,s new report "Neuroprotection - Drugs, ... This report describes the role of neuroprotection ... of the nervous system as well as in ... of the underlying mechanisms of damage to neural ...
(Date:5/28/2015)... The Academy of Model Aeronautics Foundation will be ... 15, 2015. National Model Aviation Day was created to celebrate ... flying to the general public. , The AMA has encouraged ... the celebration by hosting events in their own communities. More ... Day events. View our online event map to find ...
(Date:5/28/2015)... , May 28, 2015 Celsion Corporation ... it has received commitments from two institutional healthcare investors ... the Company,s common stock in an at-the-market registered direct ... purchase common stock. The Company entered into ... which the Company agreed to sell an aggregate of ...
Breaking Biology Technology:Global Cell Therapy Market 2015-2020 - Technologies, Markets and Companies 2Global Neuroprotection Market Report 2015-2024 - Drugs, Markets and Companies 2Global Neuroprotection Market Report 2015-2024 - Drugs, Markets and Companies 3Model flying pilots celebrate the Academy of Model Aeronautics Foundation’s Third Annual National Model Aviation Day 2Celsion Corporation Announces $8 Million At-The-Market Registered Direct Offering 2Celsion Corporation Announces $8 Million At-The-Market Registered Direct Offering 3
... OriGene Technologies, Inc.,announces its flagship products, TrueORF ... fluorescence protein) vector, enabling easy transfection,monitoring and protein ... reading frame) clones and 12,000 mouse ORF clones ... October, the 2008 Nobel Prize in chemistry was ...
... 31 SleepQuest has always,followed the essence of its ... and unexplained health problems related to lack of sleep., ... of identifying and treating sleep disorders. Sleep is a,cornerstone ... wellness factor.,Studies done by the National Commission on Sleep ...
... Biosciences now offers,the LI-COR Blot Washer for automating ... addition for Western blots. Providing consistent,reproducible incubation and ... Blot Washer is an easy to use liquid ... Up to 20 presets can,be stored in the ...
Cached Biology Technology:OriGene Announces 37,000 ORF cDNA Clones in a GFP Vector 2SleepQuest Tireless in Pursuit of Good Sleep 2LI-COR Blot Washer Automates Western Blot Processing 2
(Date:5/18/2015)... GOTHENBURG, Sweden , May 18, 2015 ... touch fingerprint sensors FPC1020, FPC1025, FPC1145, FPC1155 and FPC1035 from ... place from June until and including Q3 2015 and the ... Asia . Communicated order values for 2015 hereby amount ... revenues for Q1 2015 of 140 MSEK and a number ...
(Date:5/11/2015)... -- Through a well-rounded UAS delegation representing private industry, government, and ... at AUVSI,s Unmanned 2015 conference last week in ... UAS industry met with over 200 hundred people from ... UAS ecosystem. "Our message is clear and ... Rich Knoll . "If you want to fly UAS, ...
(Date:5/7/2015)... GOTHENBURG, Sweden , May 7, 2015 /PRNewswire/ ... new touch fingerprint sensors, FPC1022 and FPC1035, FPC,s ... FPC1022 and FPC1035 are mainly considered for integration ... decreased size gives smartphone OEMs increased possibilities to ... portfolio. The decreased size also improves possibilities for ...
Breaking Biology News(10 mins):Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2Ohio Flies High at Unmanned '15, Sets Stage for Ohio UAS Conference 2FPC Introduces its Smallest Touch Fingerprint Sensors to Date 2
... United States Patent and Trademark Office today awarded St. ... St. Jude,s invention of compositions for genetically modifying human ... most common forms of cancer in children and adults. ... cells to recognize and attack certain cells that cause ...
... researchers at the University of Massachusetts Amherst led by ... Geobacter sulfurreducens conducts electricity very effectively along ... the idea of such a novel biological electron transfer ... Geobacter ,s behavior represents a paradigm shift. It goes ...
... 2013) A team of researchers in Seoul, Korea ... in that they regenerate annually - contain multipotent stem ... veterinary medicine. The study appears as an early ... is now freely available on-line at http://www.ingentaconnect.com/content/cog/ct/pre-prints/ct0897seo . ...
Cached Biology News:St. Jude Children's Research Hospital announces issuance of US patent for cancer therapy 2UMass Amherst researchers reveal mechanism of novel biological electron transfer 2UMass Amherst researchers reveal mechanism of novel biological electron transfer 3Cell Transplantation study finds stem cells in deer antler 2
HyPro 20 system for slide hybridization. 20 slide capacity. Active vibration system, slide rack and humidity chamber. Ideal for microarray and in situ hybridization....
... interferons are cytopathic effect inhibition assays, also known ... include; tissue culture supernatents; serum from PK studies ... unit is defined as the quantity of interferon ... of viral infection by 50%. All ...
Request Info...
... Nebulizers are available in two product models - ... two models is that the 6000AT+ includes a ... drier aerosol for improved detection limits and reduced ... a range of elements by 10-40 times. Note ...
Biology Products: