Navigation Links
Are siRNA Pools Smart?

represent the mean caspase 3 activity relative to a negative control (Silencer Negative Control #1 siRNA; Ambion) transfected sample. (B) Representative results from specific genes illustrate the types of agreement and disagreement that occurred between the various siRNAs.


Cell Proliferation Results
Cell number was measured using an alamarBlue (AccuMed International) microplate-based assay. These results were confirmed by high-throughput microscopy. As shown in Figure 3A, siRNAs targeting the various kinases differentially affected cell number relative to negative control transfected samples. Different siRNAs targeting the same gene typically had similar effects on cell proliferation; however, it was common for one of the three single siRNAs to behave differently from the other two siRNAs. Similarly, siRNA pools often failed to display a phenotype consistent with the majority of the siRNAs that were individually transfected (Figure 3B).


Apoptosis Assay Results
During screens for kinases that influence apoptosis, both cell number and caspase 3 activity were measured following siRNA transfection and etoposide treatment. Assaying for cell number was necessary because some of the transfected siRNAs differentially altered cell growth or survival. Normalization of the caspase 3 activity data by cell number allowed us to express data as caspase 3 activity per cell. As expected, reducing expression of several kinases inhibited activation of caspase 3 by etoposide, suggesting that these kinases play a role
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Custom and library siRNA for efficient gene silencing
2. Custom and library siRNA for efficient gene silencing
3. Cancer siRNA Oligo Set Version 1.0
4. Library siRNA
5. Custom siRNA Oligo Synthesis Service
6. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
7. Quantification of siRNA Silencing Efficiency Using the LightCycler System
8. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
9. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
10. siRNA transfection optimization with the Agilent 2100 bioanalyzer
11. siRNA Design Guidelines
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Are siRNA Pools Smart

(Date:3/3/2015)... DUBLIN , Mar. 03, 2015 Research ... announced the addition of Jain PharmaBiotech,s new report ... to their offering. , ... viral vectors, nonviral vectors and cell therapy with ... method of drug delivery and various routes of ...
(Date:3/3/2015)... DNA Diagnostics Center (DDC), one of the largest ... 20 th anniversary today. Headed by President and ... services company that focuses on DNA testing across multiple ... generates a significant portion of sales across international markets ... Peter Vitulli acknowledges the anniversary of the ...
(Date:3/3/2015)... March 3, 2015  PDL BioPharma, Inc. (PDL or ... that the third of three shareholder lawsuits filed in ... On February 2, 2015, a securities lawsuit ... behalf of a putative class of purchasers of the ... No. 2:14-CV-01526-APG-NJK (D. Nev.), was voluntarily dismissed without prejudice.  ...
(Date:3/2/2015)... MD and Fort Washington, PA (PRWEB) March 02, 2015 ... Genetics (ASHG) and ReachMD announced today ... of audio interviews designed to educate healthcare professionals on ... management. , The series features peer-to-peer interviews ... topics such as:, ,     Translational Genomics and ...
Breaking Biology Technology:Gene Therapy Market Report 2014-2024 - Technologies, Markets and Companies 2Gene Therapy Market Report 2014-2024 - Technologies, Markets and Companies 3DNA Diagnostics Center, One of The World's Largest DNA Testing Labs, Celebrates Their 20th Anniversary 2DNA Diagnostics Center, One of The World's Largest DNA Testing Labs, Celebrates Their 20th Anniversary 3PDL BioPharma Announces Dismissal of Third and Final Shareholder Lawsuit 2American Society of Human Genetics (ASHG) and ReachMD Launch Series on Genetics and Genomics 2American Society of Human Genetics (ASHG) and ReachMD Launch Series on Genetics and Genomics 3
... 2011 Regeneron Pharmaceuticals, Inc. (Nasdaq: ... has been appointed Senior Vice President, General Counsel and ... of the Company,s legal affairs, including general corporate legal ... to Leonard S. Schleifer, M.D., Ph.D., president and chief ...
... Sept. 6, 2011 ChromaDex Corporation (OTCBB: CDXC) announced ... Jr., will be presenting the company,s business strategy and ... Global Investment Conference. The event will be held September ... City. ChromaDex is scheduled to make its ...
... announces that a new market research report ... Industrial Biotechnology China News 1108 ... In this issue, Grandhope,s getting listed successfully ... the medical device industry. And this issue ...
Cached Biology Technology:Regeneron Announces Appointment of Joseph J. LaRosa as Senior Vice President, General Counsel and Secretary 2ChromaDex to Present at the Rodman & Renshaw Annual Global Investment Conference 2Industrial Biotechnology China News 1108 2Industrial Biotechnology China News 1108 3
(Date:2/12/2015)... MINNETONKA, Minn. , Feb. 12, 2015 /PRNewswire/ ... technology company specializing in clinical study management systems, ... Partner Program , further distinguishing iMedNet ... for Clinical Research Organizations (CROs) and healthcare consultants.  ... sales support, prospective customer referrals and numerous co-marketing ...
(Date:2/11/2015)... , February 11, 2015 ... report "Access Control Market by Product (Cards and Readers, ... Military and Defense, Government, Industrial, Healthcare, Education) and By ... by MarketsandMarkets, the Access Control Market is ... growing at a CAGR of 10.6% between ...
(Date:2/9/2015)... Texas , Feb. 9, 2015  Lintec of ... fabrication methods for carbon nanotube (CNT) macrostructures, including sheets, ... at Dallas (UTD). Leveraging the vast industrial ... Tokyo, Japan , Lintec of America is ... Richardson, TX , focusing on scaling up ...
Breaking Biology News(10 mins):MedNet Solutions Announces Key Enhancements To Its iMedNet Partner Program 2Access Control Market Worth $10.4 Billion by 2020 2Access Control Market Worth $10.4 Billion by 2020 3Access Control Market Worth $10.4 Billion by 2020 4Lintec announces exclusive license to commercialize innovative carbon nanotube technology from the University of Texas at Dallas 2Lintec announces exclusive license to commercialize innovative carbon nanotube technology from the University of Texas at Dallas 3Lintec announces exclusive license to commercialize innovative carbon nanotube technology from the University of Texas at Dallas 4
... proven effectiveness thanks to their appetite-suppressing effects. A team ... "Nutrition and the Brain" in Lyon, has managed to ... describe in detail the chain reactions triggered by digesting ... after a meal. Their results, published on 5 July ...
... A traffic policeman standing at a busy intersection directing the ... but a similar scene appears to still frequently play out ... step of cell division by preventing the progress of one ... Biology Laboratory (EMBL) in Heidelberg, Germany, have found. The study ...
... coral reefs to a total ecosystem collapse lasting thousands of ... Science . The paper shows how natural climatic shifts stopped ... reef shutdown, which began 4,000 years ago, corresponds to a ... "As humans continue to pump greenhouse gases into the atmosphere, ...
Cached Biology News:The 'appetite-suppressing' effect of proteins explained 2Stop and go 2Climate change suspended reef growth for 2 millennia 2
IHC detection kit for PCNA in cells and tissues...
... Immunogen: Synthetic peptide derived from the ... protein. Specificity: Reacts with the ... Reactivity: Human Mouse (positive controls: WNT1-transfected ... lysates and mouse Mouse-1 cell lysates). ...
The pYES2 vector is designed for native expression of your protein of interest in S. cerevisiae. It contains the URA3 gene for selection in yeast and 2 origin for high-copy maintenance....
... [125I] Direct SPA Screening Assay, ... reagents that eliminate lengthy sample extraction ... (optional use).Range: 1.32-84.28 ng/ml (4-256 pmol/ml).Sensitivity: ... at 2-8 C. Category: Drug Screening ...
Biology Products: