Navigation Links
Are siRNA Pools Smart?

stance, the Cenix siRNA design algorithm--upon which Ambion's Silencer Pre-designed siRNAs are based--has a success rate of >80%, with the majority of the siRNAs providing >85% reduction in target gene expression (Figure 1). Given the success rate of individual algorithm-designed siRNAs, the benefits of siRNA pooling have decreased (Figure 2). Furthermore, the recent observations of off-target effects by siRNAs [1] suggest that combining multiple siRNAs might actually increase the chances that genes other than the target are being affected.

Figure 1. Efficacy of Silencer Pre-designed siRNAs. More than 1100 siRNAs targeting nearly 400 endogenously expressed human genes were tested for target mRNA reduction. More than 82% of the siRNAs reduced target gene expression by at least 70% and more than 61% reduced target gene expression by at least 85%. All siRNA designs were based on an algorithm developed by Cenix BioScience.

Figure 2. Target mRNA Reduction by Single siRNAs and siRNA Pools. Cells transfected with three individual Silencer Pre-designed siRNAs (30 nM) or a pool of all three siRNAs (10 nM each) were monitored for target mRNA reduction by real-time RT-PCR 48 h post-transfection. Relative target mRNA expression was taken as a percentage of mRNA expressed in cells transfected with Silencer Negative Control #1 siRNA (Ambion). All real-time
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Custom and library siRNA for efficient gene silencing
2. Custom and library siRNA for efficient gene silencing
3. Cancer siRNA Oligo Set Version 1.0
4. Library siRNA
5. Custom siRNA Oligo Synthesis Service
6. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
7. Quantification of siRNA Silencing Efficiency Using the LightCycler System
8. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
9. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
10. siRNA transfection optimization with the Agilent 2100 bioanalyzer
11. siRNA Design Guidelines
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Are siRNA Pools Smart

(Date:11/27/2014)... DUBLIN , Nov. 27, 2014 ... of the "Global Palmitic Acid Industry Report 2014" ... http://photos.prnewswire.com/prnh/20130307/600769 The Global Palmitic Acid ... on the current state of the global palmitic acid ... of the industry including definitions, classifications, applications and industry ...
(Date:11/27/2014)... Ottawa, Ontario (PRWEB) November 27, 2014 ... Korean Ministry of Food and Drug Safety (MFDS) regulatory ... The system provides genetic results in less than 60 ... system is indicated as an aid for doctors to ... 2C19 genetic pathway. These include some of the world’s ...
(Date:11/27/2014)... OXFORD, England , November 27, 2014 ... free online on ScienceDirect   Elsevier ... information products and solutions, is pleased to announce the ... Letters . The launch ... by the rapid advances at the forefront of applied ...
(Date:11/27/2014)... November 27, 2014 Calcivis, ... management of tooth decay, today announces that it ... Calcivis® Caries Activity Imaging System. The ... medical device and consumable combination designed to transform ... decay). It involves a unique, proprietary bioluminescence approach ...
Breaking Biology Technology:Global Palmitic Acid Industry Report 2014 2Spartan Bioscience Receives Korean Regulatory Approval for First Rapid DNA Test of Mutations Affecting Metabolism of Common Drugs 2Spartan Bioscience Receives Korean Regulatory Approval for First Rapid DNA Test of Mutations Affecting Metabolism of Common Drugs 3Elsevier Announces the Launch of a New Journal: Extreme Mechanics Letters 2Elsevier Announces the Launch of a New Journal: Extreme Mechanics Letters 3Calcivis Completes Clinical Study of Novel Caries Activity Imaging System 2Calcivis Completes Clinical Study of Novel Caries Activity Imaging System 3
... , March 23 Ambry Genetics today ... of genetic tests expected to pinpoint the cause of ... marks the first time next generation sequencing technology, coupled ... X testing, is being made clinically available to address ...
... PHILADELPHIA , March 23 The Superior Court of Pennsylvania has reinstated an appeal ... The appeal had been dismissed by an order from the same court on February 18, 2010 . The ... , ... ...
... ... Sharing across Kluyver’s European Academic and Industrial Research Sites , ... Basel, Switzerland (PRWEB) March ... discovery and life science research, today announced a five-year-extension of an ongoing collaboration ...
Cached Biology Technology:Ambry Genetics Launches First X-Linked Mental Retardation Diagnostics Using Next Generation Sequencing 2Ambry Genetics Launches First X-Linked Mental Retardation Diagnostics Using Next Generation Sequencing 3Ambry Genetics Launches First X-Linked Mental Retardation Diagnostics Using Next Generation Sequencing 4UPDATE: Appeal of Lawsuit by Former Chief Executive Officer- Reinstated; and Sanctions Order Vacated 2Genedata Announces Five-Year Agreement Extension with Kluyver Centre for Genomics of Industrial Fermentation 2Genedata Announces Five-Year Agreement Extension with Kluyver Centre for Genomics of Industrial Fermentation 3Genedata Announces Five-Year Agreement Extension with Kluyver Centre for Genomics of Industrial Fermentation 4
(Date:11/21/2014)... 18, 2014 According to a ... Systems (Video, RFID, Access Control, Intrusion Detection, Parking Management, ... Government), Component Service Geography - Global Forecasts to 2020", ... projected to be around $25 Billion in 2014 and ... at a CAGR of 8.69%. Browse 116 ...
(Date:11/21/2014)... -- Earlier this year Donald Spector , Chairman of ... the most prolific inventors in the world, got a ... to third party agencies. Spector envisioned this technology even ... earliest known patents in this area. Now major technology ... care, elder care and hospital applications. This technology has ...
(Date:11/21/2014)... Calif. , Nov. 20, 2014 Strict ... driver efficiency are piloting the North American and European ... of accidents growing, gesture recognition systems that are intuitive ... a mark in the industry. New ... Automotive Gesture Recognition Market in Europe ...
Breaking Biology News(10 mins):Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 2Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 3Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 4
... A team of UC Davis investigators has found that ... development of prostate cancer. The mutation of the so-called p53 ... but until now has never been shown to act as ... diagnosing and treating the disease. The study was ...
... far better competitors than their equivalents in the Indian and ... coral reefs. The picture-postcard beauty of Caribbean reefs ... contribute startling white sand to beaches. Coral reefs might seem ... constantly waged between corals and seaweeds that fight over space. ...
... NineSigma , the leading innovation partner to companies worldwide, ... NineSights ™, www.ninesights.com which is free ... NineSights is a secure and collaborative online community that ... relationships needed to drive business value. The platform allows ...
Cached Biology News:UC Davis scientists find new role for P53 genetic mutation -- initiation of prostate cancer 2NineSigma Launches NineSights, the World's First Open Innovation Social Media Destination for Innovation Seekers and Solution Providers 2NineSigma Launches NineSights, the World's First Open Innovation Social Media Destination for Innovation Seekers and Solution Providers 3