Navigation Links
An Epitope Tagging Vector for Gene Expression in Mammalian Cells

An Epitope Tagging Vector for Gene Expression in Mammalian Cells Use tag-specific antibodies to study expressed genes

Tanya Hosfield Quinn Lu
Stratagene


The pCMV-Tag1 vector is an epitope tagging vector designed for gene expression in mammalian cells. A target gene inserted into the pCMV-Tag1 vector can be tagged with the FLAG epitope (N-terminal, C-terminal or internal tagging), the c-myc epitope (C-terminal) or both the FLAG (N-terminal) and c-myc (C-terminal) epitopes. Tagged constructs generated in the pCMV-Tag1 vector can be transfected into mammalian cells, and the tagged gene product can be easily characterized using commercially available, tag-specific antibodies.

The epitope tagging technique involves fusion of a protein of interest to a peptide-epitope that is recognized by a readily available antibody. In this technique, expression of the fusion protein is monitored using a tag-specific antibody, allowing a new protein to be studied without generating a new, specific antibody to that protein. Epitope tagging can be used to localize gene products in living cells, identify associated proteins, track movement of fusion proteins within the cell, or characterize new proteins by immunoprecipitation.1-3

pCMV-Tag1 Vector for Epitope Tagging

Stratagenes pCMV-Tag1 vector is a general mammalian expression vector that contains the FLAG and c-myc epitopes positioned for either terminal or internal tagging of a target protein. The pCMV-Tag1 vector (figure 1) is derived from the pCMV-Script vector4 and contains sequences for the FLAG and c-myc epitopes. These specific epitope tags are small, not interfering with the function
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Epitope-Tagging Vectors for Functional Analysis in Yeast
2. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
3. A New C-Terminal GST Vector for Protein Production in S. pombe
4. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
5. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
6. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
7. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
8. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
9. A New Lambda Vector for Mammalian Expression
10. Versatile Reporter Vectors for Monitoring Viral Transduction
11. High-Titer Retroviral Vectors for Gene Delivery
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Epitope Tagging Vector for Gene Expression Mammalian Cells

(Date:11/22/2014)... November 21, 2014 RURO, Inc., ... provider, has released Limfinity® version 5.1.4. , RURO’s ... of Limfinity® than ever in RURO’s 8 year ... minority framework enhancement. Limfinity® version 5.1.4 is the ... and beyond bug fixes! , Limfinity® 5.1.4 release ...
(Date:11/22/2014)... , Nov. 21, 2014  The element of ... emerging diseases, i.e., they strike without warning. Until now, ... warning of a viral outbreak, resulting in suboptimal responses ... Ebola currently. Photo - http://photos.prnewswire.com/prnh/20141121/160242 ... has now been removed because virus gene structural changes ...
(Date:11/21/2014)... November 19, 2014 The science of ... was shared with more than 200 primary students in ... from the University of Otago Physics Department and funded ... Otago students, members of the university’s OSA/SPIE Student Chapter ... early September, giving children from small, rural schools hands-on ...
(Date:11/21/2014)... The report, "Synthetic Biology Market by ... Technology (Bioinformatics, Nanotechnology, Gene Synthesis, Cloning & ... Global Forecast to 2018", analyses and studies ... challenges. , Browse 99 market data tables ... and in-depth TOC of the global synthetic ...
Breaking Biology Technology:RURO Announces Limfinity® version 5.1.4 2Viruses' Advantage of Surprise is Lost 2Optics and photonics outreach programs share science, spark fascination with light 2Optics and photonics outreach programs share science, spark fascination with light 3Synthetic Biology Market Expected to Reach $5,630.4 Million in 2018 - New Report by MarketsandMarkets 2Synthetic Biology Market Expected to Reach $5,630.4 Million in 2018 - New Report by MarketsandMarkets 3Synthetic Biology Market Expected to Reach $5,630.4 Million in 2018 - New Report by MarketsandMarkets 4
... GPRO ) announced today that the Company will present ... 1, 2008 at,approximately 4:20 p.m. Eastern Time. The presentation ... through a link on the investors section of,Gen-Probe,s website ... 30 days following the event., About Gen-Probe, ...
... 24 /PRNewswire/ ETEX Corporation, an advanced,biomaterials company, ... a supply,agreement with AlloSource., (Logo: ... ETEX with Demineralized Bone Matrix (DBM),powder to ... setting,calcium phosphate to form EquivaBone(TM)., This ...
... Trubion Pharmaceuticals,Inc. (Nasdaq: TRBN ), today announced that executives ... in April and,May:, -- April 30: Morgan ... Ritz-Carlton, 455 Grand Bay Drive in Key Biscayne, FL. Dr. Peter ... to present at 9:50 a.m. EDT. The ...
Cached Biology Technology:Gen-Probe to Webcast Presentation at Morgan Stanley Global Healthcare Unplugged Conference 2ETEX Announces Supply Agreement with AlloSource 2Trubion Announces Presentations at April/May Investor Conferences 2
(Date:11/4/2014)... PA) – Temple University School of Medicine announced ... winner, an initiative funded by the Bill & ... Professor of Obstetrics, Gynecology and Reproductive Sciences; and ... Temple University School of Medicine will pursue an ... "Maternal Blood Biomarkers to Assess Fetal Neurodevelopment.", Grand ...
(Date:11/4/2014)... A newly published research study examining only marketing ... of fast food restaurants has found that the ... disproportionately exposed to such marketing tactics. , Authored ... her colleagues, the study is the first to ... interior and exterior of fast food restaurants and ...
(Date:11/4/2014)... 2014) – A majority of Madagascar,s 101 species of ... serious consequences for the rainforests they call home. A ... impacts lemurs can have on rainforest tree populations, which ... have on the region,s rich biodiversity. , A large ... by lemurs. Lemurs in turn disperse the seeds ...
Breaking Biology News(10 mins):Temple University School of Medicine receives Grand Challenges Explorations grant 2Fast food marketing for children disproportionately affects certain communities 2Fast food marketing for children disproportionately affects certain communities 3Gardeners of Madagascar rainforest at risk 2Gardeners of Madagascar rainforest at risk 3
... National Institute of Standards and Technology (NIST) announced today ... million to three universities to provide cost-shared support for ... projects were chosen from 93 applicants in a special ... $10.98 million to the University of ...
... vacancy sign is on, but the lowlifes who check ... of Florida and the University of New Mexico have ... bacteria in a manner the scientists liken to "roach ... to new coatings that will disinfect common surfaces, combat ...
... CANovember 24, 2008Researchers at The Scripps Research Institute have figured ... long and twisted tangles of DNA within a cell,s nucleus ... direct the synthesis of proteins, which have many specific functions ... published in the November 23, 2008 online issue of ...
Cached Biology News:NIST awards $24M in grants for new research facilities 2NIST awards $24M in grants for new research facilities 3NIST awards $24M in grants for new research facilities 4NIST awards $24M in grants for new research facilities 5Scientists build 'roach motel' for nasty bugs of the bacterial variety 2Scripps Research scientists shed light on how DNA is unwound so that its code can be read 2Scripps Research scientists shed light on how DNA is unwound so that its code can be read 3
... sufficient materials for 1,464 array sample elements. , , ... , Blocker BSA in PBS (10X): 50 ml ... BupH Phosphate Buffered Saline: 8 packs , 10% ... Pico Luminol Enhancer: 2 x 25 ml , SuperSignal ...
... The Partec Cell Counter Analyser counts cells ... the CCA detects only cells, so that ... the results.,While passing through a 250 m ... fluorescence light signal which is used as ...
... Obtain Clean and Accurate Results ... dramatically increase the sensitivity and accuracy of ... homogeneous cell types and multi-cellular structures isolated ... unique approach to microdissection-developed in 1996 at ...
Mouse monoclonal antibody raised against a partial recombinant DHX8. NCBI Entrez Gene ID = DHX8...
Biology Products: