Navigation Links
A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)

MS is performed.(Both are dominated by the fragment ion at m/z 207.2.)

In setting the quantitation measurement through the resulting MRM transitions (321.3 > 207.2 for MPA and 514.3 > 207.2 for MPAG), it is important to distinguish that the chromatographic peak seen at the transition 514.3 > 207.2 and corresponding to MPAG is coincident with a chromatographic peak at the same retention time, but obtained through the transition (321.3 > 207.2) specific for MPA (Figure 6). This is determined to be MPAG in-source fragmenting into free MPA, making it clear that chromatographic separation is extremely important in segregating the endogenous MPA in the specimen from the MPA derived from the in-source fragmentation of MPAG.

The modified configuration described here makes it possible to include the analytes CsA, TAC, SIR, EVE, and the additional internal standard (CsD) into the method and quantitate them simultaneously whenever they are present in the same specimen. (MPA is generally analyzed in plasma while the rest of the compounds are quantitated in whole blood). Figure 7 shows the applicability of the method to real samples. In these optimized conditions, the total run time is approximately 5 minutes per sample.

Figure 8 describes the obtained linearity for the samples in the range 0.5 15 ug/mL and Figure 9 gives proof of the intra-run reproducibility on a quality control plasma sample (Level I, Recipe-Munich, Reference Value 0.48 ug/mL). Deviation from the mean value is restricted to 0.5 %.The estimated detection limit on the API 3200 using this protocol is approximately 0.03 μg/mL plasma.

Figure 10 shows the correlation obtained when MPA quantitation is compared with a commercially available HPLC-UV assay.

Conclusion

The modified method described in this application note (1, 2) has been demonstrated to be:

<
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
2. The Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by LC/MS/MS Using an Atmospheric Pressure Photoionization Source
3. HPLC/MS/MS Analysis of Bitter Acids in Hops and Beer
4. Simultaneous Determination of Residues of Approximately 100 Pesticides and Metabolites in Fruit and Vegetables by LC/MS/MS
5. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
6. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
7. Detection of nitrofurane metabolites in food with HPLC/MS/MS
8. Simultaneously characterizing and quantifying chloramphenicol and its metabolite using LC/MS/MS
9. Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS
10. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
11. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
Post Your Comments:
(Date:9/1/2015)...   Medfusion, Inc., a patient engagement ... and patients, today announced a $3 million investment ... venture capital firms.   "We welcome ... City Venture Partners and Hatteras Venture Partners," said ... Chairman. "Both firms, experience working at the intersection ...
(Date:9/1/2015)... ... September 01, 2015 , ... ... been discovered by researchers at the Keck Graduate Institute and its collaborators. The ... , The findings were published online on August 27 by Scientific Reports, an ...
(Date:9/1/2015)... CA (PRWEB) , ... September 01, 2015 , ... ... today announced the release of a groundbreaking new version of its flagship DNA ... design methodology breaks down complex DNA sequences into numerous genetic parts, which in ...
(Date:8/31/2015)... ... August 31, 2015 , ... The Global Supply Chain Resiliency ... and resiliency practitioners with luminaries and thought leaders to advance the professional discipline ... their innovations and performance excellence. At that time its principle sponsor, Resilinc ...
Breaking Biology Technology:Medfusion Secures Funding to Continue Evolution and Advance Mobile Strategy 2Medfusion Secures Funding to Continue Evolution and Advance Mobile Strategy 3Scientists Discover New Use for an Old Therapy Inhibiting Deadly Pathogens Including Ebola and Anthrax 2Scientists Discover New Use for an Old Therapy Inhibiting Deadly Pathogens Including Ebola and Anthrax 3Scientists Discover New Use for an Old Therapy Inhibiting Deadly Pathogens Including Ebola and Anthrax 4GenoCAD Releases Combinatorial Design Tool to Optimize Protein Expression and Streamline DNA Synthesis Orders 2Global Supply Chain Resiliency Council Announces Incorporation and Web Site Launch 2Global Supply Chain Resiliency Council Announces Incorporation and Web Site Launch 3
... 19 /PRNewswire/ - Canada,s six genome centres demonstrated ... Annual Canadian Genomics Reception at the International Plant ... week. More than 150 researchers, funders and other ... of the informal networking event. , "This Canadian ...
... 2009 In 1902, the National Zoo in Washington D.C. ... thylacine, or Tasmanian Tiger, brought to the United States from ... cubs arrived at the zoo. However, by the mid-1930s, ... In a study published online today in Genome ...
... green giveaway of nutrient pollution in a lake. Scientists would ... turn different colors if exposed to biological weapons, food spoilage ... University of Florida engineering researchers have tapped the working parts ... "smart dust.",The feat, which signifies a new approach to technology ...
Cached Biology Technology:'Museomics' yields new insights into extinct Tasmanian tiger 2Researchers: Molecular forklifts overcome obstacle to 'smart dust' 2Researchers: Molecular forklifts overcome obstacle to 'smart dust' 3
(Date:8/20/2015)... Aug. 20, 2015 NXT-ID, Inc. (NASDAQ: ... company focused on the growing mobile commerce market and ... joined The Smart Card Alliance and the EMV Migration ... and the Smart Card Alliance Latin America (SCALA) Chapter ... peers and thought leaders promoting adoption of smart card ...
(Date:8/18/2015)... , Aug. 18, 2015  Navitas ... key contributor to the Submission Content Management ... IRISS, a non-profit forum. Dedicated to the Implementation ... a neutral platform for industry, vendors, health ... information and work towards a standard method ...
(Date:8/17/2015)... , Aug. 17, 2015  NXT-ID, Inc. (NASDAQ: ... company focused on the growing mobile commerce market and ... sales campaign for Q4 2015. The new marketing campaign ... consisting of Amplitude Marketing Group ( http://amplitudemarketing.com/ ) and ... is a leader in retail driven marketing and brand ...
Breaking Biology News(10 mins):NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 2NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 3NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 4Navitas, a Key Contributor in IRISS Forum Webinar 2Navitas, a Key Contributor in IRISS Forum Webinar 3NXT-ID, Inc. Launches Sales Campaign for Wocket Smart Wallet with Award Winning Marketing Team 2NXT-ID, Inc. Launches Sales Campaign for Wocket Smart Wallet with Award Winning Marketing Team 3
... key discovery into the genetics of the bacteria that ... could lead to new ways to fight the disease. ... the Biotechnology and Biological Sciences Research Council (BBSRC), found ... bacterium Erwinia carotovora, its ability to damage the plant ...
... have identified an enzyme thought to be an important ... diabetes. A chronic condition that affects nearly three million ... severe than Type 2. Type 1 diabetes, also called ... cells start destroying the beta-cells that produce insulin in ...
... COLUMBUS , Ohio -- As sea levels rise, coastal communities ... water supplies than previously thought, according to a new study ... saltwater will intrude into fresh water aquifers, given the sea ... (IPCC). The IPCC has concluded that within the next 100 ...
Cached Biology News:New research to help fight widespread potato disease 2UVa Health System team uncovers gene's role in type 1 diabetes 2Climate change could diminish drinking water more than expected 2Climate change could diminish drinking water more than expected 3
... real-time observations of cell cultures under precise, ... different strain parameters in addition to a ... strain chamber used by the ST-195 is ... ST-195 can be used with Nikon, Olympus, ...
... and biologic production demands on cell and ... viable cell populations. Therefore, both cell concentration ... determined accurately and precisely. The new Vi-CELLTM ... cost effective means to perform the trypan ...
... cell and PowerPac Basic power supply are used ... Mini Trans-Blot cell accommodates two 7.5 x 10 ... modular electrode assembly, Bio-Ice cooling unit, buffer tank, ... The PowerPac basic power supply, 100-120 and 220-240 ...
...
Biology Products: