Navigation Links
A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)

ystems.com First dimension chromatography was accomplished via an Applied Biosystems POROS R1/20, 2.1 x 30 mm perfusion-column. Second dimension chromatography was performed by a Phenomenex Luna 5 μm Phenyl- Hexyl, 2 x 50 mm column (p.n. 00B- 4257-B0) housed in an oven at 60 C.

Diluting solution consisted of 80% methanol with 1% ZnSO4 and 200 ng/mL of Cyclosporin D as internal standard. 20 ng/mL Ascomycin is added when TAC, SIR and EVE are quantified in the same sample

Results and discussion

In relation to the other immunosuppressant drugs in the 5-drug panel, there are several analytical features unique to MPA. These are discussed below.

Despite the presence of a free acidic moiety, which should make MPA very sensitive when ionized in negative ion mode, the sensitivity in positive ion mode is higher. Several papers have demonstrated that the molecule is quite labile in the ionization process (5).

Fragmentation of the MPA precursor ion (m/z 321) results in an MS/MS spectrum as shown in Figure 3, with the most intense fragment ion at m/z 207.1.

MPA is predominantly metabolized to the glucuronide form (MPAG). This glucuronidated form is at a much higher concentration (around one order of magnitude greater) than the residual MPA in the specimen.

Despite the mild source-declustering conditions, the MPAG metabolite is prone to fragmentation in the sourceinterface region. As shown in Figure 4, the MS spectrum for the MPAG metabolite shows two prominent ions corresponding to the ammoniumadduct ion [M+NH4]+ (m/z 514.2) and the glucuronide-depleted ion, which is the original MPA protonated ion [M+H]+ (m/z 321.2) produced by in-source fragmentation.

The MS/MS spectrum of MPAG (Figure 5) is directly comparable with that of pure MPA (Figure 5 v Figure 3), since the glucuronide moiety becomes a neutral loss when MS/
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
2. The Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by LC/MS/MS Using an Atmospheric Pressure Photoionization Source
3. HPLC/MS/MS Analysis of Bitter Acids in Hops and Beer
4. Simultaneous Determination of Residues of Approximately 100 Pesticides and Metabolites in Fruit and Vegetables by LC/MS/MS
5. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
6. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
7. Detection of nitrofurane metabolites in food with HPLC/MS/MS
8. Simultaneously characterizing and quantifying chloramphenicol and its metabolite using LC/MS/MS
9. Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS
10. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
11. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
Post Your Comments:
(Date:7/31/2015)... ... July 31, 2015 , ... R-Biopharm is proud ... partially hydrolyzed gluten in foods, has been accepted by AOAC International as Official ... Gluten in Fermented Cereal-Based Products by R5 Competitive ELISA,” based on a specific ...
(Date:7/30/2015)... ... ... The 2015 Market Research Report on the Global Propanol Industry ... market with a focus on the Chinese situation. Major companies included in the propanol ... Sanofi, Royal Dutch Shell, LG Chem and Zhejiang Xinhua Chemical. , The report provides ...
(Date:7/30/2015)... , July 30, 2015  With over 60,000 customers across the globe, ISN ... connect 450 hiring organizations with more than 60,000 safe and reliable contractors in over 80 countries. ... ... ... Headquartered in Dallas, TX ...
(Date:7/30/2015)... ... 2015 , ... GEA's Pony™ NS2006L is a ... to 1500 bar. The sanitary design gauge of the NS2006L is well suited ... Pony™ NS2006L homogenizer is an ideal solution for the processing of nanoparticles, ...
Breaking Biology Technology:RIDASCREEN® Competitve Gliadin Receives AOAC Official First Action Status 2Global Propanol Market Technical Data and Manufacturing Analysis 2020 2Global Propanol Market Technical Data and Manufacturing Analysis 2020 3ISN Reaches Over 60,000 Worldwide Customers 2ISN Reaches Over 60,000 Worldwide Customers 3GEA Announces the Pony™ NS2006L… a Self-contained, High Pressure Lab Homogenizer for Product Development Ensuring Maximum Efficiency and Safety 2
... Jan. 10, 2012 Saladax Biomedical, Inc., a privately ... achieve the promise of personalized medicine for new and ... six patents within the last 12 months, bringing the ... which contain broad claims in the immunoassay space.  The ...
... Show) – iHealth Lab Inc., the pioneering designer and ... Blood Pressure Dock and iHealth Digital Scale today announced ... immediately when users download latest version of the companion ... Scale, the free service will enable users to instantly ...
... Solutions Inc. (OTCBB: BLFS), a leading developer, manufacturer, and ... freeze media for cells and tissues, today announced preliminary ... 2011. Highlights include: , Sixth consecutive quarter of record ... 13% over the third quarter and 41% over the same ...
Cached Biology Technology:Saladax Biomedical, Inc. Issued Six Patents Containing Broad Claims in the Immunoassay Space 2iHealth Lab Launches Personalized Web Portal to Store Your Vitals 2BioLife Solutions 2011 Revenue Up 33% 2BioLife Solutions 2011 Revenue Up 33% 3
(Date:7/30/2015)... 2015 Cellecta, Inc., a U.S.-based provider ... and biomarker discovery, announced the release of its ... all human protein coding genes. CRISPR technology enables ... gene,s function. Cellecta,s new Human Whole Genome CRISPR ... so that researchers can investigate in a single ...
(Date:7/21/2015)... 21, 2015 Today, ZTE announced its Android ... well as expected revenues in 2015 that relate to sales of ... guidance of approximately 2,200 MSEK for 2015. Jörgen Lantto, ... smartphone manufacturer in China and we are ... 5 for Axon , its ...
(Date:7/13/2015)... Jul. 13, 2015 NXT-ID, Inc. (NASDAQ: ... authentication company focused on the growing mobile commerce ... patent 62/188684 for BEHAVIORAL-DIRECTED AUTHENTIATION METHOD ... convenient and secure method to make payments.  With ... methods, introduced with its groundbreaking voice-direct payment patent ...
Breaking Biology News(10 mins):CELLECTA, INC. Announces Launch of Human Whole Genome CRISPR Knockout Library 2FPC's Touch Fingerprint Sensor FPC1025 in ZTE's Smartphone Axon 2NXT-ID Patents Behavior-Directed Payments 2NXT-ID Patents Behavior-Directed Payments 3NXT-ID Patents Behavior-Directed Payments 4
... genetic variant that substantially improves survival of individuals with ... disease, has been indentified by a consortium of researchers ... and Robert Brown, MD, DPhil, Chair and Professor of ... of the KIFAP3 gene variant is reported in the ...
... in avian conservation is underway this spring throughout the ... A legion of highly skilled volunteer ornithologists is helping ... sites for migrating birds in the southern coastal zone ... migrants. These songbirds summer in the subarctic part of ...
... Lyncean Technologies, Inc. has just received a Phase ... Center for Research Resources (NCRR) to develop "Differential ... produced by the Compact Light Source (CLS). ... ongoing development of the Compact Light Source and ...
Cached Biology News:UMMS researchers isolate first 'neuroprotective' gene in patients with amyotrophic lateral sclerosis 2Conservationists seek to identify prime stopover sites for migrating birds 2Conservationists seek to identify prime stopover sites for migrating birds 3Lyncean Technologies Inc. receives $1.2 M from NCRR to develop new imaging technique 2
... our Regulatory Edition of Molecular Imaging (MI) ... for life science research and drug discovery ... with U.S. Food and Drug Administrations Code ... Electronic Records; Electronic Signatures [21 CFR Part ...
... time, you can identify and characterize high-value, ... interferences. The Agilent Multiple Affinity Removal System ... proteins from human serum, plasma, CSF samples ... With this revolutionary system, you can: ...
... Select Tissue Arrays are intended for laboratory ... were fixed in neutral buffered formalin and ... diagnosis was performed by certified pathologists. ... blocks and relocated into a new recipient ...
... The Agilent Two-Color RNA Spike-In Kit contains ... anneal only to complementary control probes on ... The spike-ins serve as a positive microarray ... NOTE: This kit is a required ...
Biology Products: