Navigation Links
A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)

preparation step, the required time for the entire analytical procedure significantly influences the final overall sample throughput (5).

As with the original method, a fast and inexpensive on-line solid-phase extraction (SPE) is performed through a twodimensional liquid chromatography configuration. Using this configuration, sample clean up effectively minimizes any ion suppression interference during the MRM measurements. This permits the use of Cyclosporin D (CsD) as an internal standard for quantitation of all 5 immunosuppressant drugs included in the extended panel.

Experimental conditions

Samples were prepared for MPA measurements by adding 100 μL of a diluting solution* to either 50 μL of either plasma samples, calibrators or controls, This mixture was then vortex mixed for 30 seconds. After centrifugation at 13000 rpm x 5 min., 100 μL of supernatant were transferred to either an autosampler vial or a microtiter plate.

The hardware configuration included an Applied Biosystems/MDS SCIEX API 3200TM Triple Quadrupole Mass Spectrometer equipped with a Turbo VTM source. This source operates in positive ion mode at a voltage of +5500 volts and with a Turbo V gas heated at 650 C.

Multiple Reaction Monitoring (MRM) measurements were made using declustering potential (DP) and collision energy (CE) values, which were automatically optimized by the Analyst software for each of the analytes. DP values ranged between 40 and 140 V; CE values were between 30 and 65 eV.

Two-dimension chromatography was performed through a split arrangement of the modules of the LC pumping system (either a Perkin Elmer Series 200 Micro Pump, an Agilent 1100 Binary Pump with the pump heads disconnected from the mixing tee or a Shimadzu Prominence or 10Dvp pumps) and a computer-controlled Valco Valve (10-port, 2 positions) plumbed as illustrated in Figure 2. www.appliedbios
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
2. The Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by LC/MS/MS Using an Atmospheric Pressure Photoionization Source
3. HPLC/MS/MS Analysis of Bitter Acids in Hops and Beer
4. Simultaneous Determination of Residues of Approximately 100 Pesticides and Metabolites in Fruit and Vegetables by LC/MS/MS
5. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
6. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
7. Detection of nitrofurane metabolites in food with HPLC/MS/MS
8. Simultaneously characterizing and quantifying chloramphenicol and its metabolite using LC/MS/MS
9. Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS
10. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
11. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
Post Your Comments:
(Date:9/16/2014)... , Sept. 16, 2014  The Council ... entrepreneurial support organization in the country, today released ... Report . It showed that ... North Carolina startups in the technology, ... more global investment from a variety of sources. Significant ...
(Date:9/16/2014)... Sept. 16, 2014 /PRNewswire-iReach/ -- Researchers found that ... identifies patients with bone fragility fracture when today,s ... people worldwide are at risk of fracture for ... bone strength test, the DEXA bone density scan. ... for Bone and Mineral Research Annual Conference (ASBMR) ...
(Date:9/16/2014)... RENU 28, the world's first and only skin gel ... in Australia and New Zealand in mid-August . , The ... tour will make stops in Brisbane on September 16, the ... on the 20th, and Melbourne on the 22nd. , Helping ... to educate people about the new product and business opportunity ...
(Date:9/15/2014)... JOSE, Calif. , Sept. 15, 2014 /PRNewswire/ ... Proposals (RFP) for flexible, printed product demonstrators (demos) ... a two-stage RFP process with pre-proposals due on ... January 2015.  Funds for proposals ultimately selected are ... and have a significant cost share by the ...
Breaking Biology Technology:CED's Mid-Year Update to its Innovators Report Shows NC Startups Attracting Investment From Across the Globe with Several High-Profile Investment Deals, IPOs and Acquisitions Signaling Strength of Entrepreneurial Sector 2CED's Mid-Year Update to its Innovators Report Shows NC Startups Attracting Investment From Across the Globe with Several High-Profile Investment Deals, IPOs and Acquisitions Signaling Strength of Entrepreneurial Sector 3CED's Mid-Year Update to its Innovators Report Shows NC Startups Attracting Investment From Across the Globe with Several High-Profile Investment Deals, IPOs and Acquisitions Signaling Strength of Entrepreneurial Sector 4ActiveLife's BMSi Test Provides Hope for 160 Million with Undetectable Risk of Bone Fracture 2ActiveLife's BMSi Test Provides Hope for 160 Million with Undetectable Risk of Bone Fracture 3Global Wellness Company Hosts ASEA Australia Tour September 16-22 2FlexTech Alliance Issues 2015 Request for Proposals in Flexible, Printed Electronics 2
... , NOVATO, Calif., Sept. 8 BioMarin Pharmaceutical Inc. ... Officer of BioMarin, will present a company update at the Morgan Stanley ... 1:35 p.m. ET. , , Interested parties ... investor section of the BioMarin website, www.BMRN.com . ...
... , SAN DIEGO, Sept. 8 eBioscience Corporation, a ... the acquisition of NatuTec GmbH, a leading distributor of high-quality biological ... , "For the past 10 years, NatuTec has ... R. Nelson, CEO of eBioscience. "The NatuTec acquisition provides eBioscience ...
... LAIYANG, China, Sept. 8 /PRNewswire-Asia-FirstCall/ -- ... a,U.S. pharmaceutical company with its principal operations in the ... for,fiscal year 2009. The Company expects to meet ... revenue and operating income of $40 to $43 million ...
Cached Biology Technology:BioMarin to Present at the Morgan Stanley Healthcare Conference 2eBioscience Corporation Acquires NatuTec GmbH 2Jiangbo Pharmaceuticals Reaffirms Guidance for Fiscal 2009 2
(Date:9/16/2014)... YORK (17 September, 2014)More than $100 trillion in ... of annual carbon dioxide (CO2)a 40 percent reduction ... 2050 if the world expands public transportation, walking ... report released by the University of California, Davis, ... (ITDP). , Further, an estimated 1.4 million ...
(Date:9/16/2014)... its own bacterial identity. That,s the conclusion of University ... studied the genetic fingerprints of bacteria on 57 species ... study demonstrates for the first time that host plants ... possess very different microbial communities on their leaves," said ... in the UO,s Institute of Ecology and Evolution who ...
(Date:9/16/2014)... the Keystone XL pipeline (KXL) would likely increase oil ... researchers surveyed by the non-profit organization Near Zero. The ... Climate Impact," and includes both supporters and opponents of ... oil sands could lead to significantly higher greenhouse gas ... markets respond. , "This report examines three main ...
Breaking Biology News(10 mins):Global shift away from cars saves US$100 trillion, eliminates 1,700 MT of CO2 pollution 2Global shift away from cars saves US$100 trillion, eliminates 1,700 MT of CO2 pollution 3Global shift away from cars saves US$100 trillion, eliminates 1,700 MT of CO2 pollution 4Global shift away from cars saves US$100 trillion, eliminates 1,700 MT of CO2 pollution 5Microbiome research shows each tree species has a unique bacterial identity 2Keystone XL would likely raise oil sands production and greenhouse gas emissions 2
... For the first time in nearly a century drinkers will ... heritage barley from the Victorian period. Old varieties ... the John Innes Centre revived Chevallier from the institute,s Genetic ... records indicate that the variety produced premium quality malt and ...
... analysis, carbon dioxide removal could be used under certain ... but it would not replace the bulk of actual ... allows to separate emissions control from the time and ... important for climate protection," says lead-author Elmar Kriegler. "You ...
... "Many of the great whales which once populated the oceans ... commercial hunting. The committee also worried about how tuna fishing ... And they considered reports about seal hunting and the decline ... October of that year, Congress passed the Marine Mammal Protection ...
Cached Biology News:Cheers to better beer and disease resistance 2CO2 removal can lower costs of climate protection 2CO2 removal can lower costs of climate protection 3Sea mammals find US safe harbor 2Sea mammals find US safe harbor 3Sea mammals find US safe harbor 4
Affinity Purified Rabbit Anti-mouse sRANKL Biotinylated Polyclonal Antibody...
Polyclonal antiserum raised against conjugated L-isoleucine. Available as antiserum. Amino Acid Antibodies...
... in heart tissues, and is produced ... conditions. Treatment of cells with ... TIMP-4 in some cell types, but ... require concentration of cell culture media ...
Mouse monoclonal [002-49] to C1s ( Abpromise for all tested applications). entrezGeneID: 716 SwissProtID: P09871...
Biology Products: