Navigation Links
A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)

preparation step, the required time for the entire analytical procedure significantly influences the final overall sample throughput (5).

As with the original method, a fast and inexpensive on-line solid-phase extraction (SPE) is performed through a twodimensional liquid chromatography configuration. Using this configuration, sample clean up effectively minimizes any ion suppression interference during the MRM measurements. This permits the use of Cyclosporin D (CsD) as an internal standard for quantitation of all 5 immunosuppressant drugs included in the extended panel.

Experimental conditions

Samples were prepared for MPA measurements by adding 100 μL of a diluting solution* to either 50 μL of either plasma samples, calibrators or controls, This mixture was then vortex mixed for 30 seconds. After centrifugation at 13000 rpm x 5 min., 100 μL of supernatant were transferred to either an autosampler vial or a microtiter plate.

The hardware configuration included an Applied Biosystems/MDS SCIEX API 3200TM Triple Quadrupole Mass Spectrometer equipped with a Turbo VTM source. This source operates in positive ion mode at a voltage of +5500 volts and with a Turbo V gas heated at 650 C.

Multiple Reaction Monitoring (MRM) measurements were made using declustering potential (DP) and collision energy (CE) values, which were automatically optimized by the Analyst software for each of the analytes. DP values ranged between 40 and 140 V; CE values were between 30 and 65 eV.

Two-dimension chromatography was performed through a split arrangement of the modules of the LC pumping system (either a Perkin Elmer Series 200 Micro Pump, an Agilent 1100 Binary Pump with the pump heads disconnected from the mixing tee or a Shimadzu Prominence or 10Dvp pumps) and a computer-controlled Valco Valve (10-port, 2 positions) plumbed as illustrated in Figure 2. www.appliedbios
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
2. The Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by LC/MS/MS Using an Atmospheric Pressure Photoionization Source
3. HPLC/MS/MS Analysis of Bitter Acids in Hops and Beer
4. Simultaneous Determination of Residues of Approximately 100 Pesticides and Metabolites in Fruit and Vegetables by LC/MS/MS
5. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
6. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
7. Detection of nitrofurane metabolites in food with HPLC/MS/MS
8. Simultaneously characterizing and quantifying chloramphenicol and its metabolite using LC/MS/MS
9. Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS
10. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
11. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
Post Your Comments:
(Date:6/19/2013)... , June 19, 2013  Continuing its long ... infarction (MI), Beckman Coulter , Inc. announces the ... its new Access AccuTnI+3 troponin I assay for use ... "Clinicians have depended on Beckman Coulter,s troponin ... AccuTnI+3 assay has the proven performance to continue providing ...
(Date:6/19/2013)... (PRWEB) June 19, 2013 Bayer CropScience ... first Bayer Bee Care Community Leadership Award. The award ... Congressional Reception in Washington, D.C., an event where ... to the world’s food supply. , The Bayer ... harnessed the power of the honey bee colony to ...
(Date:6/19/2013)... , June 19, 2013 Biotechnology industry pioneer ... with financial software provider BlackLine Systems  and enterprise ... SAP ) for a webinar next week entitled "No ... from BlackLine and SAP to Help Keep Its Account Reconciliations ... http://photos.prnewswire.com/prnh/20061117/LAF027LOGO ) The webinar, geared at finance, ...
(Date:6/19/2013)... Bloomington, Indiana (PRWEB) June 19, 2013 ... Valentine joined the speaking faculty at 2013’s BioLogistics ... , The conference, coordinated by Cold Chain IQ ... surrounding biologics. This “complexity” is, in part, attributed ... medicine. , “Implicit within these trends is ...
Breaking Biology Technology:Beckman Coulter Announces FDA Clearance of New Access AccuTnI+3 Troponin I Assay for the Access 2 Immunoassay System 2Beckman Coulter Announces FDA Clearance of New Access AccuTnI+3 Troponin I Assay for the Access 2 Immunoassay System 3Community Mentor Wins Inaugural Bayer CropScience Bee Care Leadership Award 2Community Mentor Wins Inaugural Bayer CropScience Bee Care Leadership Award 3Community Mentor Wins Inaugural Bayer CropScience Bee Care Leadership Award 4Amgen Joins BlackLine Systems, SAP for Webinar on Automating Account Reconciliations 2Amgen Joins BlackLine Systems, SAP for Webinar on Automating Account Reconciliations 3Amgen Joins BlackLine Systems, SAP for Webinar on Automating Account Reconciliations 4BioConvergence® Presents at BioLogistics Summit on Risk Matrix for Biosamples during Shipment 2
... Former AstraZeneca Exec to Lead Hong Kong-based subsidiary Pangu ... held biopharmaceutical company focused on discovery and development of ... for multiple disease indications, today announced that Dr. James ... of Clinical Development at aTyr Pharma and President of ...
... One Corporation (Pink Sheets: MNCN), announced today that its ... serve as the company,s Chief Financial Officer, effective March ... said, "Pat brings a strong background in finance to ... financial expertise will be great assets to Mach One ...
... Alternext US: CVM) announced today that it is ... allow drugs developed using stem cells and other biological ... their shelf life. The availability of this new ... time to market by eliminating complicated and time consuming ...
Cached Biology Technology:Dr. James Cai Joins aTyr Pharma to Drive Clinical Development in Asia 2Dr. James Cai Joins aTyr Pharma to Drive Clinical Development in Asia 3Mach One Announces Appointment of Chief Financial Officer 2CEL-SCI Corporation to Launch Aseptic Filling for Stem Cell Produced Therapies and Other Biological Products 2CEL-SCI Corporation to Launch Aseptic Filling for Stem Cell Produced Therapies and Other Biological Products 3CEL-SCI Corporation to Launch Aseptic Filling for Stem Cell Produced Therapies and Other Biological Products 4
(Date:6/19/2013)... for human consumption qualifies as a gluten-free cereal that ... scientists have confirmed in a study published in ACS, ... , Joyce Irene Boye and colleagues point out that ... alone have CD. They develop gastrointestinal and other symptoms ... contain gluten-related proteins. Boye,s team sought to expand dietary ...
(Date:6/19/2013)... fall 2012, the European Medicines Agency (EMA) approved ... first ever gene therapy for clinical use in ... had developed AAV-LPL S447X for the treatment of ... deficiency (LPLD) which affects approximately one or two ... severe, life-threatening inflammations of the pancreas. Afflicted individuals ...
(Date:6/18/2013)... Arlington environmental engineer has been awarded a $394,300 ... ensure water quality and flow in the new ... Andrew Kruzic, UT Arlington associate professor of civil ... to add monochloramine to the water in an ... pump stations and pipelines. Adding monochloramine is widely ...
Breaking Biology News(10 mins):No danger of cancer through gene therapy virus 2UT Arlington research to benefit quality, flow in 150-mile Integrated Pipeline 2
... its eruption in early June, several NASA satellites have captured ... Chilean Volcano called Puyehue-Cordn Caulle and have tracked it around ... FLICKR image gallery that shows the progression of the plume ... Complex includes the Puyehue volcano, the Cordn Caulle rift zone ...
... June 15, 2011 A first of its kind ... Energy,s Oak Ridge National Laboratory is providing a close-up ... Lignin, a major component of plant cell walls, aggregates ... of cellulosic ethanol. The exact shape and structure of ...
... that add up over time could create an evolutionary express ... researchers from the University of Pittsburgh and the University of ... the Proceedings of the National Academy of Sciences ... known as transcriptional enhancerswhich determine the when, where, and how ...
Cached Biology News:NASA satellite gallery shows Chilean volcano plume moving around the world 2NASA satellite gallery shows Chilean volcano plume moving around the world 3ORNL neutrons, simulations reveal details of bioenergy barrier 2Leaky genes put evolution on the fast track, Pitt and UW-Madison researchers find 2
... sulfate-free, powder containing carbohydrate and citrate ... Definition: One unit will convert 1.0 ... to D -glucose 6-phosphate per ... C. Chem comp: Protein ...
... Vector is specifically designed to target and ... Saccharomyces cerevisiae. Displayed proteins can be analyzed ... or putative ligands. pYD1 uses the a-agglutinin ... domains Aga1 and Aga2 to display recombinant ...
... Polyclonal Antibody against Aph-1a (Aph-1aL,S Loop ... gamma secretase is a multiprotein complex ... Pen-2; all four proteins are necessary ... secretase complex catalyzes cleavage of a ...
TGase3 (S-20)...
Biology Products: