Navigation Links
A New Lambda Vector for Mammalian Expression

nd eukaryotic expression. Libraries constructed in the ZAP Express vector can be easily screened, and individual clones can be quickly converted to the pBK-CMV vector for eukaryotic transcription directed by the cytomegalovirus (CMV) immediate early promoter; this results in mid-level expression of cloned genes in mammalian cells.

Conclusions

Stratagenes Lambda ZAP vectors are designed for simplified construction of high-titer cDNA libraries, combined with the capability for excision and recircularization of cloned insert DNA from lambda phage. With the simple in vivo excision procedure, convert either individual clones or entire lambda libraries to a plasmid format. And, the newest lambda cloning vector, the Lambda ZAP-CMV mammalian expression vector, features the pCMV-Script-EX phagemid to achieve the highest levels of expression in mammalian cells.

Acknowledgments

We thank members of the Research and Development group, the Vector group, and the Custom Library group at Stratagene for discussion and suggestions.

REFERENCES
  1. Short, J.M., et al. (1988) Nucleic Acids Res. 16: 7583-7600.

  2. Alting-Mees, M., et al. (1992) Strategies 5: 58-61.

  3. Alting-Mees, M. and Short, J.M. (1989) Nucleic Acids Res. 17: 9494.

  4. Amberg, J., et al. (1993) Strategies 6: 2-4.

  5. Mullinax, R.L. and Sorge, J.A. (1995) Strategies 8: 3-5.

  6. Hosfield, T., et al. (1997) Strategies 10: 68-69.

* U.S. Patent Nos. 5,128,256 and 5,286,636 and European Patent No. 286200


'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Highly Active and Removable AffinityTagged Lambda Phosphatase
2. Lambda DNA Extraction Protocol
3. Lambda DNA Extraction Protocol
4. A New C-Terminal GST Vector for Protein Production in S. pombe
5. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
6. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
7. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
8. Epitope-Tagging Vectors for Functional Analysis in Yeast
9. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
10. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
11. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Lambda Vector for Mammalian Expression

(Date:3/31/2015)... Materials in Society Lecture Series at   ...   Elsevier , a world-leading provider of ... home of Materials Today , announced that its ... in Society  Lecture Series . The inaugural lectures ... Conference on Materials for Advanced Technologies (ICMAT) to be ...
(Date:3/31/2015)... LAKE CITY , March 31, 2015  The ... milestone with the selection of ten proposals that ... MCRI is the first patient-led, scientifically validated funding ... Stage 2 of the MCRI included ... careful review of a large number of innovative ...
(Date:3/31/2015)... (PRWEB) March 31, 2015 Diasome Pharmaceuticals, ... licensor, SDG, Inc., that a United States patent has ... insulin therapy. , Entitled “Lipid Construct for ... composition of matter claims related to the use of ... for enabling injected insulin to more effectively reach the ...
(Date:3/30/2015)... 2015  Heidrick & Struggles (Nasdaq: HSII ), the ... and culture shaping worldwide, today  announced that ... its global Healthcare and Life Sciences practice. As ... will lead a team of more than 80 partners, principals ... and Asia serving clients in the ...
Breaking Biology Technology:Elsevier Materials Science Council Introduces the Materials in Society Lecture Series at the International Conference on Materials for Advanced Technologies (ICMAT) 2Elsevier Materials Science Council Introduces the Materials in Society Lecture Series at the International Conference on Materials for Advanced Technologies (ICMAT) 3Myeloma Crowd Research Initiative Selects 10 High-Risk Multiple Myeloma Proposals For Further Review 2Diasome Pharmaceuticals, Inc. Announces Notice of Allowance from US PTO for Liver Targeting System for Insulin 2Heidrick & Struggles Strengthens Leadership of Healthcare - Life Sciences Practice 2
... QUEBEC CITY, April 28 /PRNewswire-FirstCall/ - Atrium Innovations ... and webcast at 2:00 p.m.,Eastern time on May ... release of,Atrium,s 2008 first quarter results issued earlier ... the following numbers: 416-644-3416,514-807-8791 or 800-731-5319 or via ...
... 28 ChemDiv, Inc.,a global CRO for ... a biopharmaceutical company developing first-in-class drugs,for the ... the signing,of a collaborative discovery research agreement. ... discover small molecule ligands for biotargets,nominated by ...
... The Netherlands, April 28 , ... Brachytherapy Represents the,Next Generation in DICOM-Compatible, ... a knowledge-based leader in radiation oncology, ... a comprehensive,volume-based treatment planning system to ...
Cached Biology Technology:Atrium Innovations to Hold 2008 First Quarter Financial Results Conference Call and Webcast on May 6 2Syndexa Pharmaceuticals Corporation and ChemDiv, Inc. Announce Discovery Collaboration 2Nucletron Announces Oncentra(R) Brachy* Treatment Planning Solution for Multimodality Environment 2
(Date:3/31/2015)... --  Guidepoint , a leading global research services firm, ... TRACKER, part of the Guidepoint TRACKER suite ... device and therapeutics markets. The Post-Surgical Pain Management TRACKER ... share, and adoption rates across three major modalities used ... The Post-Surgical Pain Management TRACKER will provide length ...
(Date:3/24/2015)... , Mar. 24, 2015 Research and Markets ... the "Global Iris Recognition Market 2015-2019" report ... Global Iris Recognition market to grow at a CAGR ... This report covers the present scenario and the growth ... period 2015-2019. To calculate the market size, the report ...
(Date:3/24/2015)... , March 24, 2015   Crossmatch ... solutions, today announced that its U.are.U ® ... retailer Goorin Bros . to achieve PCI ... solution secures access to the POS terminal, protecting ... risk of data breaches. With ...
Breaking Biology News(10 mins):Guidepoint Launches Post-Surgical Pain Management TRACKER 2Guidepoint Launches Post-Surgical Pain Management TRACKER 3Global Iris Recognition Market 2015-2019 with 3M Cogent, Bioenable Technologies, Cross Match Technology, Iris ID Systems & Safran Dominating 2Crossmatch Fingerprint Readers Help Retailer Prevent Data Breaches at Point-of-Sale 2
... how an invading virus hijacks a cell,s workings by ... By manipulating cell signals, the virus destroys a defensive ... in human cell cultures, may represent a broader targeting ... scientific groundwork for developing more effective treatments for infectious ...
... 2012) Autism Speaks, the world,s leading autism science ... research grants totaling $1.1 million in funding to support ... of Autism Speaks, continued funding for novel research projects ... understanding autism," said Autism Speaks Co-founder Bob Wright. ...
... radiology treatment, prostatic artery embolization, may bring hope to ... say the group of researchers who pioneered its use. ... Radiology,s 37th Annual Scientific Meeting in San Francisco, Calif. ... many men over the age of 50, and these ...
Cached Biology News:To drive infections, a hijacking virus mimics a cell's signaling system 2Autism Speaks awards $1.1 million to fund high priority studies 2Autism Speaks awards $1.1 million to fund high priority studies 3Interventional radiology: Potential breakthrough to treat men's enlarged prostate 2Interventional radiology: Potential breakthrough to treat men's enlarged prostate 3
Request Info...
...
... The integration of 2D polyacrylamide gel ... widely regarded as a core technology platform ... resolving power for complex samples limited only ... resolve small and large proteins. Quantitative image ...
... The EC105 power supply is a highly compact, ... A single adjustment knob and one hi-lo range ... 10 volt increments from 20 to 250 volts. ... from indicating output voltage to output current. For ...
Biology Products: