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A New Lambda Vector for Mammalian Expression

nd eukaryotic expression. Libraries constructed in the ZAP Express vector can be easily screened, and individual clones can be quickly converted to the pBK-CMV vector for eukaryotic transcription directed by the cytomegalovirus (CMV) immediate early promoter; this results in mid-level expression of cloned genes in mammalian cells.

Conclusions

Stratagenes Lambda ZAP vectors are designed for simplified construction of high-titer cDNA libraries, combined with the capability for excision and recircularization of cloned insert DNA from lambda phage. With the simple in vivo excision procedure, convert either individual clones or entire lambda libraries to a plasmid format. And, the newest lambda cloning vector, the Lambda ZAP-CMV mammalian expression vector, features the pCMV-Script-EX phagemid to achieve the highest levels of expression in mammalian cells.

Acknowledgments

We thank members of the Research and Development group, the Vector group, and the Custom Library group at Stratagene for discussion and suggestions.

REFERENCES
  1. Short, J.M., et al. (1988) Nucleic Acids Res. 16: 7583-7600.

  2. Alting-Mees, M., et al. (1992) Strategies 5: 58-61.

  3. Alting-Mees, M. and Short, J.M. (1989) Nucleic Acids Res. 17: 9494.

  4. Amberg, J., et al. (1993) Strategies 6: 2-4.

  5. Mullinax, R.L. and Sorge, J.A. (1995) Strategies 8: 3-5.

  6. Hosfield, T., et al. (1997) Strategies 10: 68-69.

* U.S. Patent Nos. 5,128,256 and 5,286,636 and European Patent No. 286200


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