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A New Lambda Vector for Mammalian Expression

ssDNA mutagenesis

yes

yes

yes

yes

T3/T7 RNA polymerase promoters

T7 only

yes

yes

yes

exo-mung deletions

no

yes

yes

yes

The Lambda ZAP vectors (Table 1) are available for prokaryotic expression (the Lambda ZAP II vector), two-hybrid screening in yeast (the HybriZAP 2.1 vector), both prokaryotic and eukaryotic expression (the ZAP Express vector), and now, the new Lambda ZAP-CMV vector. The Lambda ZAP II vector is the most widely used vector for constructing cDNA libraries. Libraries cloned in this vector can be functionally screened with nucleic acid probes or antibodies. Alternatively, the library can be quickly and easily mass excised into the pBluescript SK(-) plasmid vector for analysis of transcripts via expressed sequence tag (EST) sequencing or subtraction and normalization procedures. The HybriZAP 2.1 vector offers the convenience of lambda library technology; after library construction, excision of the pAD-GAL4-2.1 plasmid vector allows cloned DNA to be screened for protein-protein interactions in vivo. The ZAP Express vector offers library construction with the capability for both prokaryotic a
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