Navigation Links
A New Lambda Vector for Mammalian Expression

st plaque size. We constructed a cDNA library in the EcoR I and Xho I sites of the Lambda ZAP-CMV vector using poly(A)+ RNA from human HeLa cells. This library contained approximately 3 x 106 primary plaques. The phage titer of the amplified library was 2 x 1010 plaque-forming units (pfu)/ml. To assess the library quality (background level and insert size), we mass excised and selected phagemid colonies by antibiotic selection on kanamycin plates. Plasmid DNA was prepared from 12 randomly picked colonies, and the DNA was digested with either EcoR I and Xho I or Not I and Kpn I restriction enzymes. All 12 clones contained a cDNA insert, ranging in size from 0.5 kb to 2.5 kb, with an average size of 1.4 kb (Figure 3). The titer of the amplified library in the Lambda ZAP-CMV vector remained constant upon storage at 4C, -80C, and in the presence of 7% DMSO.

Luciferase Expression in the pCMV-Script -EX Vector

Figure 4

To demonstrate expression levels in the pCMV-Script-EX vector, we inserted the firefly luciferase gene into the BamH I site of the MCS. This same luciferase gene was inserted into the parental pCMV-Script vector. These two constructs were used for parallel transfections of Chinese hamster ovary (CHO) cells. Cell lysates were prepared and assayed for luciferase activity. In Figure 4, cells transfected with each construct show comparable luciferase activity. We also detected luciferase protein in both samples by Western blot analysis (data not shown). To further demonstrate the functionality of the pCMV-Script-EX vector, we transfected the pCMV-Script-EX vector with luciferase gene construct in
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Highly Active and Removable AffinityTagged Lambda Phosphatase
2. Lambda DNA Extraction Protocol
3. Lambda DNA Extraction Protocol
4. A New C-Terminal GST Vector for Protein Production in S. pombe
5. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
6. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
7. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
8. Epitope-Tagging Vectors for Functional Analysis in Yeast
9. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
10. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
11. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Lambda Vector for Mammalian Expression

(Date:9/16/2014)... USA (PRWEB) September 16, 2014 “Nature ... create the features that we desire in optical design,” ... at Montana State University. “As we explore surfaces and ... of those lessons were presented in San Diego in ... Light: Light in Nature ” chaired by Shaw and ...
(Date:9/16/2014)... --The emerging field of molecular electronics could take ... enabling the construction of tiny circuits from molecular ... would take on the roles currently played by ... of researchers from five Japanese and Taiwanese universities ... small-scale electronics: a molecule called picene. In a ...
(Date:9/16/2014)... Cardioxyl Pharmaceuticals, Inc. announced today ... to be effective in animal models following oral (capsule) ... infusions that could pave the way for convenient outpatient ... at the Heart Failure Society of America,s (HFSA) 18 ... Vegas . The research, conducted in ...
(Date:9/16/2014)... Md. , Sept. 16, 2014   ... biotechnology company developing novel anti-infective biologic and drug ... and diseases, announced today the completion of the Company,s ... which highlighted its irritable bowel syndrome (IBS) ... constipation-predominant IBS (C-IBS). In the keynote ...
Breaking Biology Technology:Nature’s designs inspire research into new light-based technologies 2Nature’s designs inspire research into new light-based technologies 3Nature’s designs inspire research into new light-based technologies 4Nature’s designs inspire research into new light-based technologies 5The future face of molecular electronics 2Cardioxyl Presents Oral Heart Failure Treatment Data For Its Experimental HNO Prodrug At HFSA 18th Annual Scientific Meeting 2Synthetic Biologics' Statin-Class Candidate for Constipation-Predominant Irritable Bowel Syndrome Detailed at Company's Investor Day 2Synthetic Biologics' Statin-Class Candidate for Constipation-Predominant Irritable Bowel Syndrome Detailed at Company's Investor Day 3
... European Commission,s Joint Research Centre (JRC) has just ... a representative range of 25 different types of ... ensure consumer protection and confidence in many innovative ... most promising technological developments. By enabling a harmonised ...
... Feb. 14, 2011 New England Biolabs has expanded its ... One Taq Hot Start DNA Polymerases. Optimized blends of ... these new products offer robust amplification across a wide range ... exonuclease activity of Deep Vent increases the fidelity and robustness ...
... N.C., Feb. 14, 2011 India,s premier CRO, ... Society of India & Congress of Neurological Surgeons ...  The event was a convergence of leading neuroscientists, ... across the globe to discuss advances made in ...
Cached Biology Technology:Small material, big impact 2New England Biolabs Introduces OneTaq™ DNA Polymerase, a Robust Solution for Routine and Difficult PCR 2Max Neeman International Initiates Advisory Committee to Advance and Improve the Conduct of Complex Neurological Trials in India 2
(Date:9/16/2014)... Health at the Mailman School of Public Health are ... and prenatal exposure to two phthalates used in a ... the journal Environmental Health Perspectives . , ... levels of the chemicals, butylbenzyl phthalate (BBzP) and di-n-butyl ... increase in risk of developing asthma between age 5 ...
(Date:9/16/2014)... maternal morbidity amongst women in Australia is increased ... published today (17 September) in BJOG: An ... Australians generally enjoy high standards of living; however, ... in particular between indigenous and non indigenous Australians. ... independent impact of socioeconomic position on severe maternal ...
(Date:9/16/2014)... American Menopause Society (NAMS) has published its key, ... womenon everything from hot flashes to heart disease. ... Recommendations for Clinical Care of Midlife Women," was ... Menopause . This is the first, comprehensive set ... women freely available to all clinicians who care ...
Breaking Biology News(10 mins):Phthalates heighten risk for childhood asthma 2Phthalates heighten risk for childhood asthma 3New study examines the impact of socioeconomic position & maternal morbidity in Australia 2New study examines the impact of socioeconomic position & maternal morbidity in Australia 3
... Cambridge, Mass. - August 10, 2011 - Between conception ... meters long, looping and coiling within the tiny abdomen. ... loops into the same formationhowever, until now, it has ... experimental observations, biological and biophysical manipulations, theory, and computation, ...
... help overcome a major barrier to the use of gene ... reported development and successful initial laboratory tests of such a ... David Kaplan and colleagues note that gene therapy ... requires safe and efficient carriers or "vectors." Those carriers ...
... , Working out the structure of a complex formed ... be key in understanding how an antibiotic-producing organism controls ... example of how other antibiotic producers regulate export to ... certain microorganisms is a complex and highly regulated process, ...
Cached Biology News:Gut coils with help from its elastic neighbor 2Gut coils with help from its elastic neighbor 3Student brings home new expertise to answer question in antibiotic resistance 2Student brings home new expertise to answer question in antibiotic resistance 3
... Psychology, The University of New South Wales, Sydney, ... Perth, Australia This is the Fourth Edition of ... It is a large format version (330 ... compact 3rd edition. This product number includes ...
... Jouan RC10 series of vacuum concentrators/centrifugal evaporators ... features allied to rapid and safe concentration ... be combined with oil-free or high vacuum ... a wide selection of rotors (eliminating sample ...
... is Thermo Electron's most advanced personal gradient ... to use sophisticated onboard software and a ... to offer performance, accuracy and simplicity in ... of customer driven features built up on ...
Carrier Serum...
Biology Products: