Navigation Links
A New Lambda Vector for Mammalian Expression

restriction sites in the multiple cloning site, and the pCMV-Script-EX phagemid vector.

Figure 2

The pCMV-Script-EX vector contains 15 unique sites for cloning (Figure 2) and offers the same features as its parental pCMV-Script vector;6 gene expression driven by the CMV promoter for constitutive expression in a wide variety of cell lines;### the SV40 polyadenylation site; and the neomycin-resistance gene under control of the prokaryotic b-lactamase promoter, to provide kanamycin resistance in bacteria, as well as the SV40 early promoter, to provide G418 resistance in mammalian cells. The pCMV-Script-EX vector varies from its parental pCMV-Script vector by a 29-bp sequence downstream of the f1 origin; however, this additional sequence is in a nonfunctional region of the vector. Thus, the new pCMV-Script-EX vector is functionally equivalent to the pCMV-Script vector.

The Lambda-ZAP CMV vector is available in kits of varying components to accommodate different research needs. The Lambda ZAP-CMV XR library construction kit includes the directional cDNA synthesis kit, the Lambda ZAP-CMV XR vector, Gigapack III Gold packaging extract, host strains, and helper phage. Lambda ZAP-CMV vector kits are available that include the Lambda ZAP-CMV vector predigested with either EcoR I only or EcoR I and Xho I, host strains, and helper phage. In addition, the Lambda ZAP-CMV vector is available in undigested form with host strains and helper phage.

cDNA Library Construction in the Lambda ZAP-CMV Vector

Figure 3

Libraries constructed in the Lambda ZAP-CMV vector are characterized by high titers and robu
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Highly Active and Removable AffinityTagged Lambda Phosphatase
2. Lambda DNA Extraction Protocol
3. Lambda DNA Extraction Protocol
4. A New C-Terminal GST Vector for Protein Production in S. pombe
5. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
6. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
7. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
8. Epitope-Tagging Vectors for Functional Analysis in Yeast
9. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
10. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
11. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Lambda Vector for Mammalian Expression

(Date:5/27/2015)... Israel , May 27, 2015  Tikcro Technologies ... quarter ended March 31, 2015. ... quarter, we continued pre-clinical work to generate functional specific ... an early stage, we are encouraged by the development ... field as new antibodies which modulate immune checkpoints gain ...
(Date:5/27/2015)... , May 27, 2015  Two years after its ... hackers and citizen scientists, Oakland -based ... campaign promoting its mission to democratize science through a ... Photo ... Based out of the new ...
(Date:5/27/2015)... 27, 2015 ­ Caris Life Sciences®, a ... precision medicine, today announced it has appointed the Levine ... of excellence site in the Caris Centers of Excellence ... Cancer Institute is one of the largest cancer care ... new cancer patients annually. Derek Raghavan , M.D., ...
(Date:5/26/2015)... Kalifornien und HILDEN, Deutschland, May 26, 2015 ... Insight ®   ermöglicht ... die Erstellung dazugehöriger Berichte; Vorstellung auf den ... QIAGEN N.V. (NASDAQ: QGEN ; ... Markteinführung von QIAGEN Clinical Insight ® ...
Breaking Biology Technology:Tikcro Technologies Reports First Quarter 2015 Results 2Tikcro Technologies Reports First Quarter 2015 Results 3East Bay's Counter Culture Labs Seeks to Open Doors of Science to Public 2East Bay's Counter Culture Labs Seeks to Open Doors of Science to Public 3Caris Life Sciences Designates Levine Cancer Institute as a Caris Center of Excellence 2Caris Life Sciences Designates Levine Cancer Institute as a Caris Center of Excellence 3Caris Life Sciences Designates Levine Cancer Institute as a Caris Center of Excellence 4QIAGEN bringt klinische Bioinformatik-Plattform für Next Generation Sequencing-Anwendungen auf den Markt 2QIAGEN bringt klinische Bioinformatik-Plattform für Next Generation Sequencing-Anwendungen auf den Markt 3QIAGEN bringt klinische Bioinformatik-Plattform für Next Generation Sequencing-Anwendungen auf den Markt 4QIAGEN bringt klinische Bioinformatik-Plattform für Next Generation Sequencing-Anwendungen auf den Markt 5QIAGEN bringt klinische Bioinformatik-Plattform für Next Generation Sequencing-Anwendungen auf den Markt 6
... 3 Cardiac Science,(Nasdaq: CSCX ) ... rehabilitation, and informatics products, announced the,appointment today ... President and Chief,Operating Officer., (Logo: ... position to build our strategic capabilities at ...
... drug discovery research and high throughput screening applications, global market for ... by 2015. A key driving force is the increasing involvement of ... ... San Jose, CA (PRWEB) November 3, 2008 -- Playing ...
... researchers have synthesized an elusive metal-hydroxide compound in ... way for improved precursor inks that could boost ... to a "bottom-up" production of possibly the first ... nitroso-butylamine as an additive in place of nitrosobenzene. ...
Cached Biology Technology:Cardiac Science Appoints Dave Marver as Chief Operating Officer 2Cardiac Science Appoints Dave Marver as Chief Operating Officer 3Microplate Instrumentation & Supplies Market to Exceed $5.0 Billion by 2015, According to New Report by Global Industry Analysts, Inc 2Microplate Instrumentation & Supplies Market to Exceed $5.0 Billion by 2015, According to New Report by Global Industry Analysts, Inc 3New nanocluster to boost thin films for semiconductors 2
(Date:5/5/2015)... May 5, 2015, NXT-ID, Inc. (NASDAQ: NXTD ... on the growing mobile commerce market, reminds investors and media ... Officer, is presenting  at CARTES SECURE CONNEXIONS AMERICA 2015, held ... The three-day conference is organized into a ... in is themed Global Fraud: Where is the Trust ...
(Date:4/27/2015)... Apr. 27, 2015 Profile Solutions, Inc. (OTC: ... security systems is pleased to announce that Dr ... consultant and member of its scientific advisory board. ... as a thought leader in technology-enhanced learning models. He ... and has published studies and books focused on online ...
(Date:4/20/2015)... The announcement comes as demand for fire vault ... Ireland,s foremost records management company, has secured ... impressive track record of clients within the first 12 months, ... sector in Dubai . As a result, ... a further eight staff members at its Irish headquarters. The ...
Breaking Biology News(10 mins):NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 2NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 3Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 2Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 3Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 4Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 2Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 3
... lasers. We use them to print out documents, play CDs ... monitor our bloodstream, and even remove tumors from our bodies. ... Physics IAF in Freiburg have developed the technology for a ... of infrared laser forms the core of an analysis ...
... the sacred beetles of ancient Egypt the scarab beetle group ... of the most vulnerable representatives are contained in the flightless ... A recent study published in the open access journal ... distribution from southern Africa. The two new species ...
... has renewed its prestigious Clinical and Translational Science ... (STSI) in the amount of $29 million over ... in genomics, wireless technology and bioinformatics toward individualizing ... between Scripps Health and The Scripps Research Institute ...
Cached Biology News:Lasers offer an automated way to test drinking water 2Lasers offer an automated way to test drinking water 3NIH awards Scripps Translational Science Institute $29 million grant 2NIH awards Scripps Translational Science Institute $29 million grant 3
...
... Continuing a tradition of progressive centrifuge design ... series. The Centra-CL3 and Centra-CL3R (refrigerated) general ... laboratory., Quality Control Over Sample Preparation ... lock up to 99 protocols and has ...
PP2B-Abeta (C-20)...
... isoprostanes produced from arachidonic acid during lipid peroxidation. ... rat. 8-iso PGE2 inhibits U-46619 or I-BOP-induced platelet ... µM, respectively. When infused into the renal artery ... mg/kg/min, 8-iso PGE2 decreases the GFR and renal ...
Biology Products: