Navigation Links
A New Lambda Vector for Mammalian Expression

restriction sites in the multiple cloning site, and the pCMV-Script-EX phagemid vector.

Figure 2

The pCMV-Script-EX vector contains 15 unique sites for cloning (Figure 2) and offers the same features as its parental pCMV-Script vector;6 gene expression driven by the CMV promoter for constitutive expression in a wide variety of cell lines;### the SV40 polyadenylation site; and the neomycin-resistance gene under control of the prokaryotic b-lactamase promoter, to provide kanamycin resistance in bacteria, as well as the SV40 early promoter, to provide G418 resistance in mammalian cells. The pCMV-Script-EX vector varies from its parental pCMV-Script vector by a 29-bp sequence downstream of the f1 origin; however, this additional sequence is in a nonfunctional region of the vector. Thus, the new pCMV-Script-EX vector is functionally equivalent to the pCMV-Script vector.

The Lambda-ZAP CMV vector is available in kits of varying components to accommodate different research needs. The Lambda ZAP-CMV XR library construction kit includes the directional cDNA synthesis kit, the Lambda ZAP-CMV XR vector, Gigapack III Gold packaging extract, host strains, and helper phage. Lambda ZAP-CMV vector kits are available that include the Lambda ZAP-CMV vector predigested with either EcoR I only or EcoR I and Xho I, host strains, and helper phage. In addition, the Lambda ZAP-CMV vector is available in undigested form with host strains and helper phage.

cDNA Library Construction in the Lambda ZAP-CMV Vector

Figure 3

Libraries constructed in the Lambda ZAP-CMV vector are characterized by high titers and robu
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Highly Active and Removable AffinityTagged Lambda Phosphatase
2. Lambda DNA Extraction Protocol
3. Lambda DNA Extraction Protocol
4. A New C-Terminal GST Vector for Protein Production in S. pombe
5. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
6. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
7. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
8. Epitope-Tagging Vectors for Functional Analysis in Yeast
9. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
10. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
11. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Lambda Vector for Mammalian Expression

(Date:10/30/2014)... INDIANAPOLIS , Oct. 30, 2014 A ... Battelle Technology Partnership Practice, takes an in-depth look at ... innovation outputs that have resulted over two different time ... also provides a view of future opportunities for investment ... Indiana report shows gains in innovation ...
(Date:10/27/2014)... (PRWEB) October 27, 2014 The ... by Type (High & Medium), Sub-type (Primary & ... Processing, Infrastructure & Transportation & Power Generation) - ... the GIS market with analysis and forecasting of ... Market Data Tables with 52 Figures spread through ...
(Date:10/27/2014)... BOSTON, MA (PRWEB) October 27, 2014 ... and profit. SoundConnect , an industry leading ... for its partners and agents to deliver cloud-based ... and accelerate growth opportunities. , With SoundConnect’s ... for every twenty-five video and/or web conferencing licenses ...
(Date:10/27/2014)... 27, 2014 Investor-Edge has initiated ... NBIX ), Insmed Inc. (NASDAQ: ... ), Dyax Corporation (NASDAQ: DYAX ), and ... companies can be accessed at: http://investor-edge.com/register . ... ended at 4,483.72, up 0.69%, the Dow Jones Industrial ...
Breaking Biology Technology:New report shows Indiana's gains in life sciences innovation capital outpace the nation 2New report shows Indiana's gains in life sciences innovation capital outpace the nation 3New report shows Indiana's gains in life sciences innovation capital outpace the nation 4New report shows Indiana's gains in life sciences innovation capital outpace the nation 5Gas Insulated Switchgear Market worth $21,951.9 Million by 2019 - New Report by MarketsandMarkets 2Gas Insulated Switchgear Market worth $21,951.9 Million by 2019 - New Report by MarketsandMarkets 3Gas Insulated Switchgear Market worth $21,951.9 Million by 2019 - New Report by MarketsandMarkets 4SoundConnect Unveils Q4 Partner Incentives 2Pre-market Equity Watch on Biotech Industry - Neurocrine Biosciences, Insmed, BioCryst Pharma, Dyax, and Oncothyreon 2Pre-market Equity Watch on Biotech Industry - Neurocrine Biosciences, Insmed, BioCryst Pharma, Dyax, and Oncothyreon 3Pre-market Equity Watch on Biotech Industry - Neurocrine Biosciences, Insmed, BioCryst Pharma, Dyax, and Oncothyreon 4Pre-market Equity Watch on Biotech Industry - Neurocrine Biosciences, Insmed, BioCryst Pharma, Dyax, and Oncothyreon 5
... years is Japan. In this column, I have covered different ... 8/18/03, 3/28/05). I had the good fortune to return to ... after a three-year hiatus. , ,My last visit there ... Osaka-Chicago sister city relationship, which had started in Chicago and ...
... every company wanted to avoid: The Vapor List. Created by ... PC Letter, the List counted the weeks, then months (and ... software product was announced and when it actually shipped to ... were scrambling to put software on the then new PC ...
... - TeraMedica Healthcare Technology , a ... University of California-Davis Cancer Center in ... technology. , ,The agreement adds UC-Davis experts to ... cancer treatment product development. , ,The Evercore technology ...
Cached Biology Technology:Japan on the rebound: Implications for Midwest 2Japan on the rebound: Implications for Midwest 3Japan on the rebound: Implications for Midwest 4Japan on the rebound: Implications for Midwest 5Japan on the rebound: Implications for Midwest 6Japan on the rebound: Implications for Midwest 7Japan on the rebound: Implications for Midwest 8Japan on the rebound: Implications for Midwest 9Is Beta the new Vapor? 2Is Beta the new Vapor? 3Is Beta the new Vapor? 4
(Date:10/29/2014)... in New York City on September 19, 2014, leading ... Society Professor of Genome Sciences and Medicine at the ... of Philosophy of Tel Aviv University, was awarded the ... In addition to her honorary degree from TAU, Prof. ... School of Medicine, and for the past 18 years ...
(Date:10/29/2014)... the Barcode of Life Data Systems (BOLD). ... Data Journal (BDJ) used specimen records downloaded ... a human-readable text developed in manuscript within the ... study the species distributions of ten Nearctic species of ... originally designed to support the generation and application of ...
(Date:10/29/2014)... Genomics of the Kidney consortium ( CAGEKID ) ... exposure to aristolochic acid, an ingredient in some ... Communications , have important implications for public health. ... every year, and in Central Europe incidence rates ... International Cancer Genome Consortium (ICGC), has been studying ...
Breaking Biology News(10 mins):Geneticist and humanitarian: Prof. Mary-Claire King receives Lasker Award 2Go straight and publish: From Barcode of Life Data Systems to scholarly publishing systems 2Kidney cancer in Central Europe 2
... to cut down two-thirds of their water monitoring operation ... at Nanyang Technological University (NTU). With the world,s ... commercialised by NTU,s spin-off company, Membrane Instruments and Technology ... $200,000 each year. MINT,s new system is able ...
... PRBO Conservation Science (PRBO) and the National Aviary finds ... California helps songbirds survive through the winter, a finding ... The floodplain of California,s Central Valley is rich ... Each summer, these forests are alive with the ...
... - The vegetables in salads are chock-full of important ... without the right type and amount of salad dressing, ... trial, researchers fed subjects salads topped off with saturated, ... for absorption of fat-soluble carotenoids ╨ compounds such as ...
Cached Biology News:NTU start-up launches world's first 3-in-1 water monitoring system 2NTU start-up launches world's first 3-in-1 water monitoring system 3Restoring streamside forests helps songbirds survive the winter in California's Central Valley 2Study: No-fat, low-fat dressings don't get most nutrients out of salads 2
... a manual heat sealer ideal for low to ... can seal a wide range of plates of ... heating element of the sealer is pre-set for ... seals when used in conjunction with ABgenes sealing ...
... controller is the successor to the original DS-U1. ... of the cameras in the Nikon DS camera ... interface. The DS-U2 transmits information at high speed, ... both hub and mass storage USB 2.0 interfaces. ...
...
This unique buffer provides a gentle method for removal of primary and secondary antibodies to allow several reprobings on the same membrane. Gentle buffer will not alter the antigen for subsequent i...
Biology Products: