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A New Lambda Vector for Mammalian Expression

restriction sites in the multiple cloning site, and the pCMV-Script-EX phagemid vector.

Figure 2

The pCMV-Script-EX vector contains 15 unique sites for cloning (Figure 2) and offers the same features as its parental pCMV-Script vector;6 gene expression driven by the CMV promoter for constitutive expression in a wide variety of cell lines;### the SV40 polyadenylation site; and the neomycin-resistance gene under control of the prokaryotic b-lactamase promoter, to provide kanamycin resistance in bacteria, as well as the SV40 early promoter, to provide G418 resistance in mammalian cells. The pCMV-Script-EX vector varies from its parental pCMV-Script vector by a 29-bp sequence downstream of the f1 origin; however, this additional sequence is in a nonfunctional region of the vector. Thus, the new pCMV-Script-EX vector is functionally equivalent to the pCMV-Script vector.

The Lambda-ZAP CMV vector is available in kits of varying components to accommodate different research needs. The Lambda ZAP-CMV XR library construction kit includes the directional cDNA synthesis kit, the Lambda ZAP-CMV XR vector, Gigapack III Gold packaging extract, host strains, and helper phage. Lambda ZAP-CMV vector kits are available that include the Lambda ZAP-CMV vector predigested with either EcoR I only or EcoR I and Xho I, host strains, and helper phage. In addition, the Lambda ZAP-CMV vector is available in undigested form with host strains and helper phage.

cDNA Library Construction in the Lambda ZAP-CMV Vector

Figure 3

Libraries constructed in the Lambda ZAP-CMV vector are characterized by high titers and robu
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