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A New Lambda Vector for Mammalian Expression


The Lambda ZAP-CMV vector for efficient library construction and mammalian expression

Quinn Lu Tanya Hosfield Cherie Dewar Tim Sanchez Mike Kobrin
Stratagene

Stratagenes new lambda cloning vector, the Lambda ZAP -CMV vector,* contains the left and right arms of the Lambda ZAP II vector and the pCMV-Script -EX phagemid vector. High-efficiency cDNA libraries constructed in the Lambda ZAP-CMV vector can be converted to plasmid libraries by a simple in vivo excision procedure. This vector preserves the benefits of lambda library construction, while providing the convenience of plasmids for expression in mammalian cells and characterization of cloned inserts.

Stratagenes family of Lambda ZAP vectors is designed to simplify the construction of high-titer cDNA libraries and the characterization of inserted DNA. These vectors are distinguished by their capability to easily excise and recircularize cloned insert DNA from lambda phage. When either single clones or the entire lambda library is converted to a plasmid format by in vivo excision,1-5 they combine the high-efficiency cloning of lambda vectors with the convenience of plasmid libraries for functional studies. This elegant procedure requires very little hands-on time and eliminates the need for subcloning procedures.

High-Level Expression in Mammalian Cells

Figure 1

Stratagenes new lambda vector, the Lambda ZAP-CMV vector, offers efficient library construction and, upon mass excision, high-level eukaryotic expression in the pCMV-Script-EX phagemid vector. The Lambda ZAP-CMV vector (Figure 1) contains lambda arms derived from the Lambda ZAP II vector, three unique
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