Navigation Links
A New Lambda Vector for Mammalian Expression


The Lambda ZAP-CMV vector for efficient library construction and mammalian expression

Quinn Lu Tanya Hosfield Cherie Dewar Tim Sanchez Mike Kobrin
Stratagene

Stratagenes new lambda cloning vector, the Lambda ZAP -CMV vector,* contains the left and right arms of the Lambda ZAP II vector and the pCMV-Script -EX phagemid vector. High-efficiency cDNA libraries constructed in the Lambda ZAP-CMV vector can be converted to plasmid libraries by a simple in vivo excision procedure. This vector preserves the benefits of lambda library construction, while providing the convenience of plasmids for expression in mammalian cells and characterization of cloned inserts.

Stratagenes family of Lambda ZAP vectors is designed to simplify the construction of high-titer cDNA libraries and the characterization of inserted DNA. These vectors are distinguished by their capability to easily excise and recircularize cloned insert DNA from lambda phage. When either single clones or the entire lambda library is converted to a plasmid format by in vivo excision,1-5 they combine the high-efficiency cloning of lambda vectors with the convenience of plasmid libraries for functional studies. This elegant procedure requires very little hands-on time and eliminates the need for subcloning procedures.

High-Level Expression in Mammalian Cells

Figure 1

Stratagenes new lambda vector, the Lambda ZAP-CMV vector, offers efficient library construction and, upon mass excision, high-level eukaryotic expression in the pCMV-Script-EX phagemid vector. The Lambda ZAP-CMV vector (Figure 1) contains lambda arms derived from the Lambda ZAP II vector, three unique
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Highly Active and Removable AffinityTagged Lambda Phosphatase
2. Lambda DNA Extraction Protocol
3. Lambda DNA Extraction Protocol
4. A New C-Terminal GST Vector for Protein Production in S. pombe
5. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
6. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
7. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
8. Epitope-Tagging Vectors for Functional Analysis in Yeast
9. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
10. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
11. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Lambda Vector for Mammalian Expression

(Date:7/3/2015)... ... July 03, 2015 , ... ... applications such as animal waste reduction, bioremediation, wastewater treatment, aquaculture and grease, ... announced today that it will be showcasing, ManureMagic™, its industry-leading product for ...
(Date:7/3/2015)... , July 3, 2015 ... till 22 september 2015 på  http://www.openinnovationinscience.at ... Innovation in Science" som hålls i ... en färsk undersökning med internationella forskare och ... (LBG) är hälsovetenskapens två största utmaningar bristen ...
(Date:7/2/2015)... , ... July 03, 2015 , ... Cuvettes have been used in countless labs for ... to find the cuvette they needed. As expected, this was a daunting and tiring ... wrong part because they were not able to navigate the complex catalog structure. , Now ...
(Date:7/2/2015)... ... ... Sleepless nights will become obsolete as Luzi , a sophisticated smart ... June 23. Sound sleepers and the sleep deprived both will love Luzi for its ... integration of the Bluetooth speaker, alarm clock and ability to charge various devices, including ...
Breaking Biology Technology:DryLet to Highlight Benefits of Patented ManureMagic™ at North American Manure Expo in Chambersburg, PA July 14-15, 2015 2Lansering av världens första program för öppen innovation för att utbilda forskare 2Lansering av världens första program för öppen innovation för att utbilda forskare 3New Cuvette E-Commerce Website Launched by FireflySci 2Luzi Smart Lamp Recently Launched Indiegogo Campaign 2Luzi Smart Lamp Recently Launched Indiegogo Campaign 3
... industry seems to have taken a deep breath and a step back, getting ... were announced were mostly due to the overall economic difficulty facing the United ... ... the worst is past for health care, it does seem as though the ...
... spectacularly inefficient in their use of energy and materials, ... use of 20 major manufacturing processes. , Overall, new ... times bigger consumers of energy, per pound of output, ... making microchips uses up orders of magnitude more energy ...
... Warner Chilcott Limited (Nasdaq: WCRX ) announced ... submitted a New Drug Application for WC 3016, a ... Administration. About Warner Chilcott , Warner ... on the women,s healthcare and dermatology segments of the ...
Cached Biology Technology:The MedZilla Report: Febraury 2009 Employment Outlook for Biotech/Pharma/Health 2The MedZilla Report: Febraury 2009 Employment Outlook for Biotech/Pharma/Health 3MIT: 'Alarming' use of energy in modern manufacturing methods 2MIT: 'Alarming' use of energy in modern manufacturing methods 3Warner Chilcott Announces NDA Submission for Low Dose Oral Contraceptive 2Warner Chilcott Announces NDA Submission for Low Dose Oral Contraceptive 3
(Date:7/2/2015)... 2015 Fingerprint Cards has received an ... FPC1155 from the distributor World Peace Industrial Group (WPI), part ... Asia . Deliveries are planned to take ... used by smartphone manufacturers in Asia . ... communicated revenue guidance of approximately 2,200 MSEK for 2015. ...
(Date:6/26/2015)... ATL Technology, LLC, a top provider of electromechanical ... use solutions, headquartered in Springville, Utah , ... California corporation with locations in Santa ... Rica ). This acquisition will incorporate MedConx,s ... ATL Technology,s existing facility in Costa Rica ...
(Date:6/24/2015)... -- The biologics safety testing market is growing at ... include growth of the pharmaceutical and biological industries and ... years, the number of drug approvals in North American ... approved 225 drug applications and Health Canada approved 99. ... accounted for 3,822 of the pharmaceutical and 1,998 ...
Breaking Biology News(10 mins):FPC Receives Order for Touch Fingerprint Sensors of 268 MSEK 2ATL Technology Announces Acquisition of MedConx, Inc. and Expanded Global Footprint 2Biological Safety Testing Market by Application, Product, & Test - Global Forecast to 2019 2Biological Safety Testing Market by Application, Product, & Test - Global Forecast to 2019 3Biological Safety Testing Market by Application, Product, & Test - Global Forecast to 2019 4
... for over half of vertebrate species, but while groups such ... by scientists for decades, knowledge about relationships among many types ... A team of scientists led by Richard Broughton, associate ... at the University of Oklahoma, published two studies that dramatically ...
... by simply feeding algae genetically engineered with a vaccine?, That,s ... after they demonstrated last May that algae can be engineered ... follow up study, published online today in the scientific journal ... Not yet, although the same method may work as a ...
... long been known that certain proteins, known as transcription ... read from DNA. As a result, it is ... underlie the mechanisms responsible for evolutionary adaptation. The ... an important part in evolutionary changes to animals, appearance ...
Cached Biology News:Something's fishy in the tree of life 2Quest for edible malarial vaccine leads to other potential medical uses for algae 2Quest for edible malarial vaccine leads to other potential medical uses for algae 3Quest for edible malarial vaccine leads to other potential medical uses for algae 4A surprising new function for small RNAs in evolution 2
Borrelia Burgdorfen (IgG) -Ab EIA Sample Size: 100 l...
... pending) is a protein based inhibitor of ... the most common and troublesome RNases including ... SUPERaseIn can be used in any application ... is ideal for in vitro transcription and ...
... Inhibitor is a potent noncompetitive ... as RNase A. Like other ... Inhibitor is an acidic protein ... kDa. RNaseOUT inhibits RNase A ...
...
Biology Products: