Navigation Links
A New C-Terminal GST Vector for Protein Production in S. pombe


Versatile eukaryotic expression and protein purification vector

Quinn Lu Tanya Hosfield
Stratagene

Stratagene has constructed and tested the new pESP-3 vector, which can be used for C-terminal fusion with glutathione-S-transferase (GST) and for protein production in Schizosaccharomyces pombe. After expression and purification of the GST fusion protein using the ESP yeast protein expression and purification system, the GST tag can be removed by proteolytic cleavage with thrombin or enterokinase.

Expression and purification of heterologous genes often involve fusion of the gene of interest with a purification tag at either the N-terminus or C-terminus. The Schistosoma japonicum GST gene product has been popularly used as a protein fusion tag for affinity purification of GST fusion proteins.1 Stratagenes ESP yeast protein expression and purification system includes the pESP-1 and pESP-2 vectors for N-terminal tagging with GST.2-4 The fission yeast S. pombe, which is used in the ESP system, possesses characteristics that closely resemble those of higher eukaryotic organisms regarding chromosome structure and function, cell cycle control and RNA splicing.5 Stratagenes ESP system features high-level protein production in S. pombe, thereby retaining the posttranslational modifications of eukaryotic proteins that may be critical for their structure and function.

The availability of an expression vector for C-terminal tagging with GST is desirable since the stability and activity of fusion proteins may vary with the location of the tag.6-8 In addition, by tagging a protein at the C-terminus, only fully translated proteins will have the GST affinity tag, ensuring purification of full-length fusion proteins. Stratagene has constructed
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
2. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
3. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
4. Epitope-Tagging Vectors for Functional Analysis in Yeast
5. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
6. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
7. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
8. A New Lambda Vector for Mammalian Expression
9. Versatile Reporter Vectors for Monitoring Viral Transduction
10. High-Titer Retroviral Vectors for Gene Delivery
11. Improved Vectors for PathDetect Trans-Reporting Systems
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Terminal GST Vector for Protein Production pombe

(Date:9/16/2014)... , Sept. 16, 2014  Donald Fresne, President and ... of Stephan Vagner , PhD to its Scientific Advisory ... of Toulouse, France , performed research at ... , and at Inserm, the French institute of health and ... works both at the Curie Institute, Orsay, France ...
(Date:9/16/2014)... N.C. , Sept. 16, 2014  The ... oldest entrepreneurial support organization in the country, today ... Innovators Report . It showed ... North Carolina startups in the ... drew more global investment from a variety of ...
(Date:9/16/2014)... September 16, 2014 Pharma ... to position themselves for their 89th year ... and encoding, Pharma Packaging Solutions is preparing ... and software and new procedures. Establishing training ... Manufacturing Practices (cGMP), Pharma Packaging Solutions will ...
(Date:9/16/2014)... Sept. 16, 2014  LABSCO, the leading specialty ... to hospitals, physician office laboratories and alternate healthcare ... 1, 2014, it will serve as Siemens Healthcare ... segments and as a semi-exclusive sales distributor in ... United States . LABSCO will ...
Breaking Biology Technology:New Appointment to the Scientific Advisory Committee at Egenix, Inc. 2CED's Mid-Year Update to its Innovators Report Shows NC Startups Attracting Investment From Across the Globe with Several High-Profile Investment Deals, IPOs and Acquisitions Signaling Strength of Entrepreneurial Sector 2CED's Mid-Year Update to its Innovators Report Shows NC Startups Attracting Investment From Across the Globe with Several High-Profile Investment Deals, IPOs and Acquisitions Signaling Strength of Entrepreneurial Sector 3CED's Mid-Year Update to its Innovators Report Shows NC Startups Attracting Investment From Across the Globe with Several High-Profile Investment Deals, IPOs and Acquisitions Signaling Strength of Entrepreneurial Sector 4Adding Serialization Capabilities and Expanded Capacity, Pharma Packaging Solutions Is Preparing for 89th Year of Business 2LABSCO Secures Alliance With Siemens Healthcare Diagnostics 2LABSCO Secures Alliance With Siemens Healthcare Diagnostics 3
... , , , ... RA (TM), a new online talk show hosted by award-winning journalist and ... , , To view the Multimedia News Release, go ... ) , , New Way RA ...
... , SCOTTSDALE, Ariz., July 29 ... subsidiary of IR BioSciences Holdings, Inc. (OTC Bulletin Board: IRBS), ... is both active on oral administration and provides therapeutic effects ... Preliminary analysis of ongoing bioavailability studies in rats ...
... , , SOUTH SAN FRANCISCO, Calif., ... reported financial results for the second quarter ended June 30, 2009. , ... was $3.5 million, with a net loss of $22.9 million, including non-cash ... from operations was $1.9 million for the second quarter and $10.2 million ...
Cached Biology Technology:Video: New Way RA(TM) Talk Show Premieres Online for People Living With Rheumatoid Arthritis (RA) 2Video: New Way RA(TM) Talk Show Premieres Online for People Living With Rheumatoid Arthritis (RA) 3Video: New Way RA(TM) Talk Show Premieres Online for People Living With Rheumatoid Arthritis (RA) 4ImmuneRegen BioSciences Confirms Homspera's Therapeutic Anti-Influenza Effect on Pandemic H1N1 Influenza Virus After Oral Administration 2ImmuneRegen BioSciences Confirms Homspera's Therapeutic Anti-Influenza Effect on Pandemic H1N1 Influenza Virus After Oral Administration 3Sunesis Reports Financial Results for the Second Quarter 2009 2Sunesis Reports Financial Results for the Second Quarter 2009 3Sunesis Reports Financial Results for the Second Quarter 2009 4Sunesis Reports Financial Results for the Second Quarter 2009 5Sunesis Reports Financial Results for the Second Quarter 2009 6Sunesis Reports Financial Results for the Second Quarter 2009 7Sunesis Reports Financial Results for the Second Quarter 2009 8Sunesis Reports Financial Results for the Second Quarter 2009 9Sunesis Reports Financial Results for the Second Quarter 2009 10Sunesis Reports Financial Results for the Second Quarter 2009 11
(Date:9/16/2014)... has its own bacterial identity. That,s the conclusion of ... who studied the genetic fingerprints of bacteria on 57 ... "This study demonstrates for the first time that host ... strategies possess very different microbial communities on their leaves," ... researcher in the UO,s Institute of Ecology and Evolution ...
(Date:9/16/2014)... The fall marks a new school year, a new ... International Space Station. In September, SpaceX,s Dragon spacecraft is ... supplies and investigations as part of the company,s fourth ... cargo will be the third suite of research investigations ... in Space (CASIS). With the role of managing the ...
(Date:9/16/2014)... years after the Hesperides vessel returned to Spain culminating ... have an increasingly clear picture of how the global ... the input of pollutants from the atmosphere is not ... most remote areas of the planet, and it is ... are presented this week in CSIC Residence for Researchers ...
Breaking Biology News(10 mins):Microbiome research shows each tree species has a unique bacterial identity 2CASIS research set for launch aboard SpaceX mission to space station 2CASIS research set for launch aboard SpaceX mission to space station 3The Malaspina Expedition confirms that pollution reaches even the most remote areas of the ocean 2The Malaspina Expedition confirms that pollution reaches even the most remote areas of the ocean 3
... CORVALLIS, Ore. The soils in large areas of the ... America, have been drying up in the past decade, a ... ever examine "evapotranspiration" on a global basis. Most climate ... water from the land to the atmosphere, would increase with ...
... 9, 2010 Help may be on the way for ... the invasion of stink bugs. Scientists have reported a key ... stinkbugs, which are emerging as a major nuisance to homeowners ... a natural substance in a fungus that infects a common ...
... have reported direct evidence that taking up smoking results in ... results were reported at the 35th Congress of the European ... link between smoking and cancer has been established for decades, ... Institute of Cancer Studies, Birmingham, who presented the results. Smoking ...
Cached Biology News:Land 'evapotranspiration' taking unexpected turn: huge parts of world are drying up 2Land 'evapotranspiration' taking unexpected turn: huge parts of world are drying up 3Progress toward first commercial repellent for East Coast's stinker 2Study reveals cancer-linked epigenetic effects of smoking 2
... Synthesis Service provides you with ... knockdown any gene. We offer ... pairs with terminal dT nucleotides), ... lengths of your choice. Each ...
... System is ideal for developing single and ... system contains the new, improved, and user ... user can design specific templates for commercial ... fully integrated graphing and data regression capabilities; ...
Polyclonal Antibody to HADHB...
3T3 cells (RSV-transformed) Source: Cell Lysate Form: Ready to Use Application: Western Blotting Control...
Biology Products: