Navigation Links
A Further Step in Understanding Apoptosis,,, Direct Detection of PARP Cleavage

easily employed in many different applications such as flow cytometry, immunocytochemistry, and immunohistochemistry (also in conjunction with an anti-fluorescein antibody). Particularly, in regard to tissue sections, the antibody is a valuable tool in acquiring additional information on the results achieved using the TUNEL method (even in dual-labeling applications).

Typical Experiment
Flow cytometric analysis of PARP cleavage and TUNEL reactivity

HeLa cells were induced to undergo apoptosis via stimulation with anti-CD95 (clone 2R2, 0.2 g/ml) and cycloheximide (10 g/ml). After 5 hours, the untreated cells (Figure 1, left panel) and CD95-stimulated cells (Figure 1, right panel) were stained for PARP cleavage using the anti-PARP Caspase Cleavage Product-Fluorescein antibody (1 g/ml), and analyzed for TUNEL staining using the In Situ Cell Death Detection Kit, TMR red.
Cells in the two right boxes of the dot-blot analysis display PARP-1 cleavage; the upper two boxes indicate TUNEL-positive cells (Figure 1, right panel). Note that PARP cleavage precedes DNA degradation and TUNEL labeling. The staining pattern of the apoptotic small PARP fragment in HeLa cells is also shown by immunofluorescence in Figure 2a. A peptide competition control experiment is included (Figure 2b).

To gain new insights into apoptotic pathways particularly in situ Roche Applied Science now offers the anti- PARP Caspase Cleavage Product-Fluorescein antibody. The antibody can serve as an early marker of apoptosis since it is directed against the caspase cleavage site of PARP-1 and specifically recognizes an epitope that is generated by caspases, but is not accessible in nonapoptotic cells.
This easy-to-use add
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
3. Constructing Directional cDNA Libraries from Limited Amounts of RNA
4. Generate High-Quality, Directional Plasmid cDNA Libraries
5. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
6. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
7. TripleMaster PCR System: Direct Comparison with Competitors
8. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
9. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
10. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
11. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
Post Your Comments:
(Date:1/15/2014)... 15, 2014   Niagara Thermal Products is proud ... BioBlower Technologies LLC ("B3") as the critical thermal component ... from the Army Corps of Engineers.  B3 has developed ... biological contaminants and destroys VOCs and other chemical pollutants, ...
(Date:1/15/2014)... This webinar will focus on EMA and FDA ... biosimilars. , Regulatory frameworks are evolving many countries to ... complex nature of biopharmaceuticals makes the demonstration of biosimilarity ... Based on the specific aspects of biosimilar drug development ...
(Date:1/15/2014)... January 15, 2014 DTS Language Services, ... Online Web Portal for Life Science organizations who need document ... the subject matter of their documents in advance with a ... reduce time-to-delivery of translations, often a critical factor in clinical ...
(Date:1/14/2014)... Date: Friday, April 11, 2014 , Time: 6 ... Almshouse Road, Warrington, Pa. , Details: The Hepatitis ... finding a cure for hepatitis B and improving the quality ... Crystal Ball on Friday, April 11 at Warrington Country Club ...
Breaking Biology Technology:Niagara Thermal Products LLC Selected as Critical Supplier for Buffalo BioBlower Air Purification Systems 2Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2DTS Improves Efficiency for Life Science Document Translations 2Hepatitis B Foundation to Host Annual Crystal Ball Gala 2
... a bill Friday designed to promote capital investment and ... loans. The legislation, SB 261 , was only ... Ted Kanavas, R-Brookfield, to hit the Assembly floor this ... the Wisconsin Capital Companies Program, (WISCAP) is a revised ...
... MILWAUKEE - The benefits of switching to voice over ... meeting at the Italian Community Center in Milwaukee. Nearly ... their experiences with VoIP. , ,Clear reasons for switching ... and Pat Scheckel, VoIP practice director for Berbee Information ...
... MADISON - Inacom Education , announced today that it ... of Microsoft Technology Trainers United Training. United Training ... Technology training centers from around the country who have met ... membership. , , In order to qualify for ...
Cached Biology Technology:Wisconsin Assembly passes capital investment bill, revised CAPCO program not presented 2Wisconsin Assembly passes capital investment bill, revised CAPCO program not presented 3VoIP: Are we there yet? 2
(Date:4/24/2014)... in Munich have identified the structural features that ... viral and endogenous RNAs in living cells. , ... cellular metabolism and hijack cellular resources for the ... on viral RNA molecules that are delivered directly ... newly synthesized in the host cell, and provide ...
(Date:4/24/2014)... study of animal intelligence to-date finds that animals with bigger ... week in the journal Proceedings of the National Academy ... history of research aimed at understanding the animal mind. Specifically, ... and use tools, read social cues, or even understand basic ... of animal intelligence have focused on only one or a ...
(Date:4/24/2014)... could be improved with new insights into the make-up ... studying the Amazon Basin have revealed unprecedented detail of ... region by comparing satellite maps with hundreds of field ... assess more accurately the amount of carbon each tree ... offset schemes, in which trees are given a cash ...
Breaking Biology News(10 mins):Viral infections: Identifying the tell-tale patterns 2Viral infections: Identifying the tell-tale patterns 3Animals with bigger brains, broader diets have better self control 2Animals with bigger brains, broader diets have better self control 3Amazon rainforest survey could improve carbon offset schemes 2
... Ill. The overuse of nitrogen fertilizers in agriculture ... An international team of scientists aims to lessen ... similar plants boost their nitrogen production, even in areas ... Center of Plant Genomics and Biotechnology at the Technical ...
... fires and urban and agricultural areas in California is ... to a recent study published in the journal ... ecologist from the U.S. Forest Service,s Pacific Southwest Research ... at Devils Postpile National Monument in the eastern Sierra ...
... A potential new way to fight obesity-related illness has ... at the Case Western Reserve University School of Medicine. ... Cleveland Clinic Foundation and Cornell University, discovered the essential ... brought on by obesity. "The implications of our findings ...
Cached Biology News:Strengthening legumes to tackle fertilizer pollution 2Air pollution diminishing air quality at Devils Postpile National Monument 2Vitamin E identified as potential weapon against obesity 2Vitamin E identified as potential weapon against obesity 3
Mouse monoclonal [TU-20] to beta III Tubulin (FITC) ( Abpromise for all tested applications). entrezGeneID: 10381 SwissProtID: Q13509...
Phospho-FKHR (Thr24)/FKHRL1 (Thr32) Antibody...
Mouse anti-Insulin-Like-3 Class: Antibody Product Group: Adipokine and Obesity related...
... BRF2 Immunogen Synthetic peptide: QAARQAATSVPNPP, corresponding ... BRF2. Reactivity / Specificity Cross-reacts with ... Background Information BRF2 is one of the ... factor complex required for transcription of genes with ...
Biology Products: