Navigation Links
A Further Step in Understanding Apoptosis,,, Direct Detection of PARP Cleavage

Detection of early apoptotic events has become quite relevant since many studies have demonstrated that abnormalities in cell death regulation cause a variety of diseases and pathological conditions accompanied either by cell accumulation (e.g., cancer) or by cell loss (e.g., Alzheimers dementia).

One of the key initiation elements of the apoptotic pathway is the activation of caspases (cysteinyl-aspartic acid proteases) followed by cleavage of the caspase substrates [1]. The 113-kDa poly(ADP-ribose) polymerase 1 (PARP-1), which is normally involved in DNA repair, DNA stability, and other cellular events, is cleaved by members of the caspase family during early apoptosis. The zincdependent eukaryotic DNA-binding protein PARP-1 specifically recognizes DNA strand breaks produced by various genotoxic agents. Detection of a 89-kDa or 24-kDa caspase cleavage fragment of PARP-1 was shown to be a hallmark of apoptosis [2]. In contrast to measuring active caspase 3, which is degraded during apoptosis via the ubiquitin/proteosome pathway, measuring PARP-1 cleavage might allow sustained signal detection even in late stages of apoptosis.

Product Description

With the anti-PARP Caspase Cleavage Product Antibody you will specifically detect only the small apoptotic PARP-1 cleavage fragment, but not the full-length protein, in samples of human, mouse, or rat origin. The fluorescein- conjugated monoclonal antibody (clone PAR-16) enables direct detection of this early apoptosis marker.

Furthermore it provides a tool for apoptotic-pathway analysis (in situ) at the stage of caspase activity. It can be
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
3. Constructing Directional cDNA Libraries from Limited Amounts of RNA
4. Generate High-Quality, Directional Plasmid cDNA Libraries
5. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
6. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
7. TripleMaster PCR System: Direct Comparison with Competitors
8. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
9. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
10. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
11. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
Post Your Comments:
(Date:3/3/2015)... DUBLIN , Mar. 03, 2015 Research ... announced the addition of Jain PharmaBiotech,s new report ... to their offering. , ... viral vectors, nonviral vectors and cell therapy with ... method of drug delivery and various routes of ...
(Date:3/3/2015)... DNA Diagnostics Center (DDC), one of the largest ... 20 th anniversary today. Headed by President and ... services company that focuses on DNA testing across multiple ... generates a significant portion of sales across international markets ... Peter Vitulli acknowledges the anniversary of the ...
(Date:3/3/2015)... March 3, 2015  PDL BioPharma, Inc. (PDL or ... that the third of three shareholder lawsuits filed in ... On February 2, 2015, a securities lawsuit ... behalf of a putative class of purchasers of the ... No. 2:14-CV-01526-APG-NJK (D. Nev.), was voluntarily dismissed without prejudice.  ...
(Date:3/2/2015)... MD and Fort Washington, PA (PRWEB) March 02, 2015 ... Genetics (ASHG) and ReachMD announced today ... of audio interviews designed to educate healthcare professionals on ... management. , The series features peer-to-peer interviews ... topics such as:, ,     Translational Genomics and ...
Breaking Biology Technology:Gene Therapy Market Report 2014-2024 - Technologies, Markets and Companies 2Gene Therapy Market Report 2014-2024 - Technologies, Markets and Companies 3DNA Diagnostics Center, One of The World's Largest DNA Testing Labs, Celebrates Their 20th Anniversary 2DNA Diagnostics Center, One of The World's Largest DNA Testing Labs, Celebrates Their 20th Anniversary 3PDL BioPharma Announces Dismissal of Third and Final Shareholder Lawsuit 2American Society of Human Genetics (ASHG) and ReachMD Launch Series on Genetics and Genomics 2American Society of Human Genetics (ASHG) and ReachMD Launch Series on Genetics and Genomics 3
... 2011 Regeneron Pharmaceuticals, Inc. (Nasdaq: ... has been appointed Senior Vice President, General Counsel and ... of the Company,s legal affairs, including general corporate legal ... to Leonard S. Schleifer, M.D., Ph.D., president and chief ...
... Sept. 6, 2011 ChromaDex Corporation (OTCBB: CDXC) announced ... Jr., will be presenting the company,s business strategy and ... Global Investment Conference. The event will be held September ... City. ChromaDex is scheduled to make its ...
... announces that a new market research report ... Industrial Biotechnology China News 1108 ... In this issue, Grandhope,s getting listed successfully ... the medical device industry. And this issue ...
Cached Biology Technology:Regeneron Announces Appointment of Joseph J. LaRosa as Senior Vice President, General Counsel and Secretary 2ChromaDex to Present at the Rodman & Renshaw Annual Global Investment Conference 2Industrial Biotechnology China News 1108 2Industrial Biotechnology China News 1108 3
(Date:2/12/2015)... MINNETONKA, Minn. , Feb. 12, 2015 /PRNewswire/ ... technology company specializing in clinical study management systems, ... Partner Program , further distinguishing iMedNet ... for Clinical Research Organizations (CROs) and healthcare consultants.  ... sales support, prospective customer referrals and numerous co-marketing ...
(Date:2/11/2015)... , February 11, 2015 ... report "Access Control Market by Product (Cards and Readers, ... Military and Defense, Government, Industrial, Healthcare, Education) and By ... by MarketsandMarkets, the Access Control Market is ... growing at a CAGR of 10.6% between ...
(Date:2/9/2015)... Texas , Feb. 9, 2015  Lintec of ... fabrication methods for carbon nanotube (CNT) macrostructures, including sheets, ... at Dallas (UTD). Leveraging the vast industrial ... Tokyo, Japan , Lintec of America is ... Richardson, TX , focusing on scaling up ...
Breaking Biology News(10 mins):MedNet Solutions Announces Key Enhancements To Its iMedNet Partner Program 2Access Control Market Worth $10.4 Billion by 2020 2Access Control Market Worth $10.4 Billion by 2020 3Access Control Market Worth $10.4 Billion by 2020 4Lintec announces exclusive license to commercialize innovative carbon nanotube technology from the University of Texas at Dallas 2Lintec announces exclusive license to commercialize innovative carbon nanotube technology from the University of Texas at Dallas 3Lintec announces exclusive license to commercialize innovative carbon nanotube technology from the University of Texas at Dallas 4
... proven effectiveness thanks to their appetite-suppressing effects. A team ... "Nutrition and the Brain" in Lyon, has managed to ... describe in detail the chain reactions triggered by digesting ... after a meal. Their results, published on 5 July ...
... A traffic policeman standing at a busy intersection directing the ... but a similar scene appears to still frequently play out ... step of cell division by preventing the progress of one ... Biology Laboratory (EMBL) in Heidelberg, Germany, have found. The study ...
... coral reefs to a total ecosystem collapse lasting thousands of ... Science . The paper shows how natural climatic shifts stopped ... reef shutdown, which began 4,000 years ago, corresponds to a ... "As humans continue to pump greenhouse gases into the atmosphere, ...
Cached Biology News:The 'appetite-suppressing' effect of proteins explained 2Stop and go 2Climate change suspended reef growth for 2 millennia 2
IHC detection kit for PCNA in cells and tissues...
... Immunogen: Synthetic peptide derived from the ... protein. Specificity: Reacts with the ... Reactivity: Human Mouse (positive controls: WNT1-transfected ... lysates and mouse Mouse-1 cell lysates). ...
The pYES2 vector is designed for native expression of your protein of interest in S. cerevisiae. It contains the URA3 gene for selection in yeast and 2 origin for high-copy maintenance....
... [125I] Direct SPA Screening Assay, ... reagents that eliminate lengthy sample extraction ... (optional use).Range: 1.32-84.28 ng/ml (4-256 pmol/ml).Sensitivity: ... at 2-8 C. Category: Drug Screening ...
Biology Products: