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A Better Way to Isolate RNA AND Protein From the Same Sample

ed siRNA sequence. (B) 5 x 105 Schneider's Drosophila cells were treated for 48 hours in a 6 well plate with 10 nM of the indicated dsRNA prepared with Ambion's MEGAscript RNAi Kit. Total RNA and protein were isolated with the PARIS Kit and analyzed by Northern blot and Western blot.

Figure 5. Efficient Cellular Fractionation. (A) RNA and protein were isolated from 106 HeLa cells using the PARIS Kit. To allow direct comparison between RNA isolated from different cellular compartments, the same volume of each fraction (~5%) was analyzed by denaturing agarose gel electrophoresis, Northern blot, or Western blot. (B) 3 x 105 HeLa cells were transfected in duplicate in 6 well plates with 1 g of luciferase mRNA and the relative luciferase activity in each protein fraction was measure 18 hours after transfection. Luciferase mRNA was prepared with Ambion's mMESSAGE mMACHINE and Poly(A) Tailing Kits.


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Ordering Information Cat# Product Name Size 1921 PARIS Kit 50 purifications
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