Navigation Links
A Better Way to Isolate RNA AND Protein From the Same Sample

ed siRNA sequence. (B) 5 x 105 Schneider's Drosophila cells were treated for 48 hours in a 6 well plate with 10 nM of the indicated dsRNA prepared with Ambion's MEGAscript RNAi Kit. Total RNA and protein were isolated with the PARIS Kit and analyzed by Northern blot and Western blot.

Figure 5. Efficient Cellular Fractionation. (A) RNA and protein were isolated from 106 HeLa cells using the PARIS Kit. To allow direct comparison between RNA isolated from different cellular compartments, the same volume of each fraction (~5%) was analyzed by denaturing agarose gel electrophoresis, Northern blot, or Western blot. (B) 3 x 105 HeLa cells were transfected in duplicate in 6 well plates with 1 g of luciferase mRNA and the relative luciferase activity in each protein fraction was measure 18 hours after transfection. Luciferase mRNA was prepared with Ambion's mMESSAGE mMACHINE and Poly(A) Tailing Kits.


back to top


Ordering Information Cat# Product Name Size 1921 PARIS Kit 50 purifications
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Better RNA Amplification
2. Designing a Better siRNA
3. RNA Amplification Just Got Better: MessageAmp II
4. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
5. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
6. Isolate It All: siRNA miRNA Total RNA Native Protein
7. Isolate Total RNA and Protein From the Same Sample
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. A New C-Terminal GST Vector for Protein Production in S. pombe
10. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
11. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Better Way Isolate RNA AND Protein From the Same Sample

(Date:5/27/2015)... -- Northwest Biotherapeutics, Inc. (NASDAQ: NWBO ) ("NW Bio"), ... cancer, announced today that Dr. Marnix L. Bosch ... making an Industry Expert Theater Presentation at the 2015 ... on Saturday, May 30, 2015, from 3 to 4 ... Place. Dr. Bosch will provide an overview ...
(Date:5/27/2015)... 2015  RXi Pharmaceuticals Corporation (NASDAQ: RXII ... developing innovative therapies primarily in the areas of ... status of the Company,s Series A and Series ... http://photos.prnewswire.com/prnh/20130917/NE80755LOGO At March ... of Series A Preferred Stock and Series A-1 ...
(Date:5/26/2015)... Regenestem Network has announced the ... Buenos Aires, Argentina. The clinic will be lead by ... has assembled a multidisciplinary team of medical professionals who ... Puerto Madero office. , The new Regenestem Network ... clinical studies in cellular therapies, and establish Regenestem protocols ...
(Date:5/26/2015)... and HILDEN, Germany , May ... (NASDAQ: QGEN ; Frankfurt Prime Standard: QIA) ... Clinical Insight ® (QCI™) bioinformatics content and ... and report on genomic variants identified in next-generation ... the bioinformatics platform are in oncology, for somatic ...
Breaking Biology Technology:NW Bio To Present Overview Of DCVax-Direct Phase I Trial In Chicago This Weekend 2NW Bio To Present Overview Of DCVax-Direct Phase I Trial In Chicago This Weekend 3RXi Pharmaceuticals Provides an Update on Series A and Series A-1 Preferred Stock 2RXi Pharmaceuticals Provides an Update on Series A and Series A-1 Preferred Stock 3Regenestem Network and Silvina Pastrana, M.D. Announce the Opening of a New Stem Cell Clinic in Buenos Aires 2Regenestem Network and Silvina Pastrana, M.D. Announce the Opening of a New Stem Cell Clinic in Buenos Aires 3QIAGEN Launches Clinical Bioinformatics Platform for Next-Generation Sequencing 2QIAGEN Launches Clinical Bioinformatics Platform for Next-Generation Sequencing 3QIAGEN Launches Clinical Bioinformatics Platform for Next-Generation Sequencing 4QIAGEN Launches Clinical Bioinformatics Platform for Next-Generation Sequencing 5
... Growth Rates Under 1 Percent Since 2000,Partnership Will Target Acceleration and ... ... Banos, Philippines (vocus) March 3, 2009 -- DuPont and ... partnership to boost rice yields. The Scientific Know-How and Exchange ...
... 3 Zymetis Inc., a biotechnology company focused on ... today announces it was named a 2009 GoingGreen East ... "greentech" companies in the Eastern U.S. and Canada. ... given to emerging companies creating new green technology businesses. ...
... Informed Medical Communications (IMC), a leading provider of ... announced the appointment of Kerry Crawford to the ... this critical role, Kerry will lead operations and ... relationship management systems. This key addition is ...
Cached Biology Technology:DuPont and International Rice Research Institute Partner to Boost Rice Yields 2DuPont and International Rice Research Institute Partner to Boost Rice Yields 3Zymetis Inc. Named Top 50 'Cleantech/Greentech' Company 2Informed Medical Communications Appoints New Senior Manager, Strategic Operations 2
(Date:5/5/2015)... May 5, 2015, NXT-ID, Inc. (NASDAQ: NXTD ... on the growing mobile commerce market, reminds investors and media ... Officer, is presenting  at CARTES SECURE CONNEXIONS AMERICA 2015, held ... The three-day conference is organized into a ... in is themed Global Fraud: Where is the Trust ...
(Date:4/20/2015)... , April 20, 2015 Huntington Memorial ... Valley to implant a new miniaturized, wireless monitoring sensor ... is the first and only FDA-approved heart failure monitoring ... admissions when used by physicians to manage heart failure. ... that is implanted in the pulmonary artery (PA) during ...
(Date:4/13/2015)... According to a recently ... & Opportunities, 2020", the global biometrics market is ... till 2020. The biometrics market is garnering attention ... review of biometric management systems. The constant development ... greater efficiency, are resulting in increasing installation of ...
Breaking Biology News(10 mins):NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 2NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 3Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2
... under attack by toxic seaweed do what anyone might do when ... in the journal Science shows that threatened corals send ... noxious alga which can kill the coral if not promptly ... evidence that these "mutualistic" fish respond to chemical signals from the ...
... This press release is available in German . ... in multicellular organisms. Because of their potent effects, the production ... That is the key finding of a new study performed ... Biology. They identified a new component that modulates the production ...
... 126,000 sites across the U.S. have contaminated groundwater that requires ... "complex," meaning restoration is unlikely to be achieved in the ... a new report from the National Research Council. The ... these sites ranges from $110 billion to $127 billion, but ...
Cached Biology News:Corals attacked by toxic seaweed use chemical 911 signals to summon help 2Corals attacked by toxic seaweed use chemical 911 signals to summon help 3Corals attacked by toxic seaweed use chemical 911 signals to summon help 4MicroRNAs in plants: Regulation of the regulator 2MicroRNAs in plants: Regulation of the regulator 3Report: Cleanup of some contaminated groundwater sites unlikely for decades 2Report: Cleanup of some contaminated groundwater sites unlikely for decades 3
Recombinant Rat Leptin, CF...
Rat whole blood (Sprague-Dawley, averaging 7-8 weeks of age, either sex) is collected at the time of slaughter and refrigerated within 6 hours, and processed and frozen within 24 hours. The serum is ...
Polaroid black & white positive, 3 x 4 inch pack film, ISO 3000/ 36 ASA DIN (20 expsures). For use in the Polaroid Direct Copy Camera (PHC34)....
Applications: (+) Immunocytochemistry and immunohistochemistry...
Biology Products: