Navigation Links
A Better Way to Isolate RNA AND Protein From the Same Sample

E permits transition of previously frozen samples to a state compatible with handling at room temperature and standard homogenization methods (see the article"Stop Grinding Frozen Tissue" in this issue). As shown in Figure 3, the PARIS procedure is compatible with both of these tissue collection/RNA stabilization reagents for both cells and tissue. After homogenization, protein samples are readily available for applications that do not require native functional protein, such as Western blot analysis.

Figure 2. 2D Analysis of Proteins Isolated Using the PARIS Kit. Protein samples were resolved using a pH 4-7 IPG gel followed by 8-16% SDS-PAGE. Top: Total protein (~25 g) from HeLa cells stained with SYPRO Ruby. Bottom: Total protein (~125 g) from mouse brain stained with Coomassie Blue.

Figure 3. RNA and Protein Isolation from Stabilized Samples. (A) Isolation from ~30 mg of mouse liver, brain or kidney 5 days after collection. Samples were either kept frozen at -80C, stored in RNAlater at 4C, or snap frozen and then stored in RNAlater-ICE at -20C. (B) Isolation from 106 HeLa cells freshly harvested, kept 2 days in RNAlater at RT, or in RNAlater-ICE at -20C.

Figure 4. Analysis of the RNAi Effect. (A) 5 x 104 HeLa cells were transfected in a 24 well plate with the indicated plasmids. 48 hours after transfection, total RNA and protein were isolated with the PARIS Kit and analyzed by denaturing agarose gel electrophoresis, Northern blot, or Western blot. SCR represents a scrambl
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Better RNA Amplification
2. Designing a Better siRNA
3. RNA Amplification Just Got Better: MessageAmp II
4. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
5. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
6. Isolate It All: siRNA miRNA Total RNA Native Protein
7. Isolate Total RNA and Protein From the Same Sample
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. A New C-Terminal GST Vector for Protein Production in S. pombe
10. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
11. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Better Way Isolate RNA AND Protein From the Same Sample

(Date:9/30/2014)... Hungary , September 30, 2014 ... and intuitive browsing of chemistry in documents ... chemistry software solutions and consulting services for life ... chemical information and generate Markush structures from documents. ... an organizations intellectual property, however generating and understanding ...
(Date:9/30/2014)... Calif. , Sept. 30, 2014 FemCap ... women,s health announces its receipt of a patent No: ... menstrual cup has also received FDA clearance in the ... and the CE in Europe. This patent is related to ... with this issuance, the company is releasing FemmyCycle Teen, ...
(Date:9/30/2014)... Boston, MA (PRWEB) September 30, 2014 ... communications (UC) and collaboration company is dedicated to ... data connectivity needs. Julie Power, a ... team to ensure collaboration solutions stays connected and ... response to the increased movement of businesses to ...
(Date:9/30/2014)... September 30, 2014 Back in the 1970’s, ... square feet. per employee, which included space for circulation and ... says Jeff Howell, partner at Nidea Corporate Real Estate ... Toronto. “By the year 2000, however, 250 square feet ... that number come down to 175 to 225 square feet, ...
Breaking Biology Technology:ChemAxon Introduces ChemCurator for Chemical Information Extraction and Markush Structure Creation 2The FemmyCycle 2SoundConnect Expands Carrier Services Division 2ITRA Global Reports on How Companies Are Squeezing More Employees into Less Office Space 2ITRA Global Reports on How Companies Are Squeezing More Employees into Less Office Space 3
... 2011 AAPC ( www.aapc.com ) announced its ... again lowered prices from the previous year. Prices have been reduced ... primary purpose of a price reduction was to benefit our more ... the significant savings," said Olga Starynskaya, coding books department manager for ...
... Perceptive Informatics , the industry,s leading eClinical solutions provider ... PRXL ), today announced results of a global ... Response Adaptive Trials." The webinar, presented by Perceptive Informatics ... professionals from the biopharmaceutical industry across a broad range ...
... America, Inc . ( http://www.cordblood-america.com ) (OTC Bulletin Board: ... preservation company focused on bringing the life saving potential ... to announce that its shareholders have approved an amendment to ... The vote was approximately 80% for the stock ...
Cached Biology Technology:AAPC Lowers Prices on Medical Coding Books, Again 2Perceptive Informatics Global Survey Results Show Growing Industry Interest in Implementing Adaptive Trial Designs 2Perceptive Informatics Global Survey Results Show Growing Industry Interest in Implementing Adaptive Trial Designs 3Perceptive Informatics Global Survey Results Show Growing Industry Interest in Implementing Adaptive Trial Designs 4Perceptive Informatics Global Survey Results Show Growing Industry Interest in Implementing Adaptive Trial Designs 5Cord Blood America: Shareholders Approve Common Stock Reverse Split 2
(Date:9/30/2014)... What do seedless watermelon, salmon, and strawberries all have ... have two sets of chromosomes and are diploid, these ... more sets of chromosomesseedless watermelon and salmon have 3 ... 10! While this might seem surprising, in fact ... was first discovered over a century ago, but only ...
(Date:9/30/2014)... Prenatal exposure to tetrachloroethylene (PCE) in drinking water ... abruption, according to a new study led by ... , The study, published in the journal ... 1,019 unexposed pregnancies among 1,766 women in Cape ... late 1960s to the early 1980s by the ...
(Date:9/30/2014)... involving mice and published in the October 2014 issue ... , suggests that just because you can do it, ... of Italian scientists have found that using marijuana in ... system. This damage may result in autoimmune diseases and ... disease and rheumatoid arthritis in adulthood. , "I hope ...
Breaking Biology News(10 mins):Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 2Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 3Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 4Contaminated water linked to pregnancy complications, BU study finds 2Adolescent exposure to thc may cause immune systems to go up in smoke 2
... a cold or flu knows, a high temperature is ... immune reaction when fighting off pathogens. Laboratory studies, in ... in detail, have shown that these responses display significant ... the maximum possible level of intensity? New immunological theories ...
... about positive and negative emotions they often use spatial metaphors. A ... person is down in the dumps. Some researchers believe these metaphors ... do we use spatial words to talk about emotional states, we ... and emotion To test this link between vertical space ...
... two license agreements with SuperPower, a wholly-owned subsidiary ... intellectual property on second generation (2G) high temperature ... Sponsored Research Agreement (SRA) previously executed between the ... by Venkat Selvamanickam, M.D. Anderson chair professor of ...
Cached Biology News:Only some like it hot 2To remember the good times, reach for the sky 2SuperPower and UH sign high temperature superconducting wire license agreements 2
Anti-Dynamin Monoclonal Antibody Description: 100 g Research Focus: neuroscience Storage: -20C Shipping Temperature: 4C...
Carrier-Free Recombinant Human Flt-3/Flk-2 Ligand (Flt3/Flk2 Ligand, Flt3L, Flt3-L, Flk2L, Flk2-L) 100 ug...
Anti-CD74 Monoclonal Antibody Description: 200 l Research Focus: cell surface antigens Storage: 4C Shipping Temperature: 4C...
POU domain, class 2, transcription factor 2,...
Biology Products: