Navigation Links
A Better Way to Isolate RNA AND Protein From the Same Sample

p://www.ambion.com/catalog/CatNum.php?1906">DNA-free DNase Treatment and Removal Reagents (available separately) are ideal to quickly remove trace amounts of DNA from the total and nuclear RNA samples without phenol extraction or alcohol precipitation. The cytoplasmic RNA fraction is virtually free of DNA contamination.

Each protein fraction can also be used directly for most common applications, including functional assays, immunoprecipitation, Western blotting or two-dimensional gel electrophoresis (Figure 2 below). For experiments such as gel shift assays or enzymatic assays, protein samples are usually concentrated enough to be diluted in the final reaction buffer. The Cell Disruption Buffer and Cell Fractionation Buffer have been specially designed to be compatible with downstream analyses.

The PARIS Kit includes sufficient reagent to perform 50 purifications. The kit also comes with a comprehensive Instruction Manual and RNase-free microfuge tubes, glass fiber filters and reagents for RNA isolation. Each purification can accommodate 1 to 75 mg of tissue or 100 to 107 cells.

PARIS is Compatible with RNAlater and RNAlater-ICE Treated Tissue.
Many researchers use the RNA stabilization reagent RNAlater to protect RNA integrity immediately after cell or tissue collection, to stabilize RNA during long period of storage, or to circumvent the need to grind frozen tissue into a powder prior to RNA isolation. (See the article "RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater" for some of its many applications.) Ambion's new RNAlater-IC
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Better RNA Amplification
2. Designing a Better siRNA
3. RNA Amplification Just Got Better: MessageAmp II
4. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
5. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
6. Isolate It All: siRNA miRNA Total RNA Native Protein
7. Isolate Total RNA and Protein From the Same Sample
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. A New C-Terminal GST Vector for Protein Production in S. pombe
10. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
11. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Better Way Isolate RNA AND Protein From the Same Sample

(Date:7/3/2015)... , ... July 03, 2015 , ... The global failure ... 7.52% from 2015 to 2020. The bioscience market is expected to grow at the ... use of failure analysis equipment in the research labs and academic institutions for root ...
(Date:7/3/2015)... NEW YORK , July 3, 2015  Pomerantz ... filed against Puma Biotechnology, Inc. ("Puma" or the "Company")(NYSE: ... class action, filed in United States District Court, Central ... under 15-cv-00865, is on behalf of a class consisting ... between July 23, 2014 and May 13, 2015 inclusive ...
(Date:7/2/2015)... ELLYN, Ill. , July 2, 2015 ... Colton Eakins are gaining invaluable real-world experiences ... of Technology this summer. The students will ... Dr. Jeff Wereszczynski,s research group at IIT. The ... Dr. Tom Carter , Physics; Dr. Kathy ...
(Date:7/2/2015)... Calif. (PRWEB) , ... July 02, 2015 , ... Sleepless ... improve an individual’s quality of sleep, launched its Indiegogo campaign on June 23. Sound ... Luzi's voice command technology makes it effortless to use, while the integration of the ...
Breaking Biology Technology:Failure Analysis Market to Grow at 7.52% CAGR to 2020 2Failure Analysis Market to Grow at 7.52% CAGR to 2020 3Failure Analysis Market to Grow at 7.52% CAGR to 2020 4Failure Analysis Market to Grow at 7.52% CAGR to 2020 5SHAREHOLDER ALERT: Pomerantz Law Firm Reminds Shareholders with Losses on their Investment in Puma Biotechnology, Inc. of Class Action Lawsuit and Upcoming Deadline - PBYI 2SHAREHOLDER ALERT: Pomerantz Law Firm Reminds Shareholders with Losses on their Investment in Puma Biotechnology, Inc. of Class Action Lawsuit and Upcoming Deadline - PBYI 3SHAREHOLDER ALERT: Pomerantz Law Firm Reminds Shareholders with Losses on their Investment in Puma Biotechnology, Inc. of Class Action Lawsuit and Upcoming Deadline - PBYI 4COD Students Participate in Prestigious Summer Internships 2Luzi Smart Lamp Recently Launched Indiegogo Campaign 2Luzi Smart Lamp Recently Launched Indiegogo Campaign 3
... identified, search engines are leading the way for consumers ... related to the efficacy of these search engines, among ... soon and why could this happen? , ,If search ... todays search results, which often include a great deal ...
... mentioned my first law of softwareany mildly successful software program ... operating system. Youve seen it. The e-mail system that gets ... in version three and so on. The ERP system that ... for this kind of behavior is the browser. , ,So ...
... campaign season, and you've been poked and prodded by television ... John Kerry drank a beer at your neighborhood bar and ... Dick Cheney was spotted more in Wisconsin in 2004 than ... years. John Edwards has been in the state so often ...
Cached Biology Technology:When search engines collide 2When search engines collide 3When search engines collide 4The first law of software 2The first law of software 3Twenty ways you can tell this campaign has finally pushed you over the edge 2
(Date:6/24/2015)... YORK , June 24, 2015 This ... market, over the next six years. It contains an ... the industry, along with their impact from the short, ... It also discusses the industry, market, and technology trends ... that the need of concerned authorities to efficiently manage ...
(Date:6/23/2015)... DUBLIN , June 22, 2015 ... has announced the addition of the "Body-Worn ... Share, Growth, Trends and Forecast 2014 - 2020" ... report offers strategic analysis of the global body-worn ... market has been segmented on the basis of ...
(Date:6/17/2015)... GERMANTOWN, Maryland , June 17, 2015 /PRNewswire/ ...   Produktlinie mit DNA-Tests verbessert den Analyseprozess und senkt ... ; Frankfurt Prime Standard: QIA) hat heute in den ... Investigator ® für die ... neuen Kits zur Erstellung genetischer Fingerabdrücke bieten eine integrierte ...
Breaking Biology News(10 mins):Automatic Identification System Market by Class, by Platform, by Application and by Geography - Global Forecasts & Analysis to 2014 - 2020 2Body-Worn Temperature Sensors Market - Global Industry Analysis 2QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 2QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 3QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 4QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 5QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 6
... such as schizophrenia, major depression and cancer are ... dysfunctional genes. A novel computational biology method developed ... PhD, http://www.njit.edu/news/2008/2008-367.php , associate professor in NJIT,s ... a way to uncover the critical genes responsible ...
... WEST LAFAYETTE, Ind. Although research has linked inbreeding ... of animals, a new study finds it plays no ... Purdue University researchers recently examined 2,000 adult and ... deformities, mainly consisting of missing, extra or dwarfed digits ...
... NEW YORK, NY (October 28, 2008) The first ... exploring the role of hospital touch surfaces in the ... researchers reported their findings in a poster session at ... in Washington, D.C. on Tuesday, October 28. The ...
Cached Biology News:NJIT professor finds engineering technique to identify disease-causing genes 2Study rules out inbreeding as cause of amphibian deformities 2
Mouse monoclonal [7E10] to C Peptide ( Abpromise for all tested applications). entrezGeneID: 3630 SwissProtID: P01308...
...
... Antigen peptide transporter 2 ... (Peptide transporter PSF2) (Peptide ... (Peptide transporter involved in ... Antigen: ...
... Recognizes Nabeta2. The epitope specific ... in any other known proteins.,SPECIES ... to work on human because ... conserved (17/19 residues). Reactivity with ...
Biology Products: