Navigation Links
A Better Way to Isolate RNA AND Protein From the Same Sample

p://www.ambion.com/catalog/CatNum.php?1906">DNA-free DNase Treatment and Removal Reagents (available separately) are ideal to quickly remove trace amounts of DNA from the total and nuclear RNA samples without phenol extraction or alcohol precipitation. The cytoplasmic RNA fraction is virtually free of DNA contamination.

Each protein fraction can also be used directly for most common applications, including functional assays, immunoprecipitation, Western blotting or two-dimensional gel electrophoresis (Figure 2 below). For experiments such as gel shift assays or enzymatic assays, protein samples are usually concentrated enough to be diluted in the final reaction buffer. The Cell Disruption Buffer and Cell Fractionation Buffer have been specially designed to be compatible with downstream analyses.

The PARIS Kit includes sufficient reagent to perform 50 purifications. The kit also comes with a comprehensive Instruction Manual and RNase-free microfuge tubes, glass fiber filters and reagents for RNA isolation. Each purification can accommodate 1 to 75 mg of tissue or 100 to 107 cells.

PARIS is Compatible with RNAlater and RNAlater-ICE Treated Tissue.
Many researchers use the RNA stabilization reagent RNAlater to protect RNA integrity immediately after cell or tissue collection, to stabilize RNA during long period of storage, or to circumvent the need to grind frozen tissue into a powder prior to RNA isolation. (See the article "RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater" for some of its many applications.) Ambion's new RNAlater-IC
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Better RNA Amplification
2. Designing a Better siRNA
3. RNA Amplification Just Got Better: MessageAmp II
4. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
5. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
6. Isolate It All: siRNA miRNA Total RNA Native Protein
7. Isolate Total RNA and Protein From the Same Sample
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. A New C-Terminal GST Vector for Protein Production in S. pombe
10. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
11. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Better Way Isolate RNA AND Protein From the Same Sample

(Date:11/21/2014)... The science of light and how it ... than 200 primary students in a traveling program organized ... Otago Physics Department and funded by an SPIE ... the university’s OSA/SPIE Student Chapter visited six primary schools ... from small, rural schools hands-on lessons in optics and ...
(Date:11/21/2014)... The report, "Synthetic Biology ... Cloning kits), Technology (Bioinformatics, Nanotechnology, Gene Synthesis, ... Bioremediation) - Global Forecast to 2018", analyses ... opportunities, and challenges. , Browse 99 market ... 185 pages and in-depth TOC of the ...
(Date:11/21/2014)... 2014 Deep Research Report on Global ... and in-depth research report on the Sodium ferrocyanide ... its definition, classification, application, and industry chain structure ... the international market analysis, including China’s domestic market ... Europe, Asia, China, Japan etc. regions) industry analysis ...
(Date:11/21/2014)... and SAN DIEGO , Nov. 20, ... announced that President and CEO Marc Hedrick , M.D. will ... DATE:   Thursday, December 4, 2014 TIME:    11:15 ... here or paste this URL into your browser,s address ... investors pre-register to save time and receive event updates. ...
Breaking Biology Technology:Optics and photonics outreach programs share science, spark fascination with light 2Optics and photonics outreach programs share science, spark fascination with light 3Synthetic Biology Market Expected to Reach $5,630.4 Million in 2018 - New Report by MarketsandMarkets 2Synthetic Biology Market Expected to Reach $5,630.4 Million in 2018 - New Report by MarketsandMarkets 3Synthetic Biology Market Expected to Reach $5,630.4 Million in 2018 - New Report by MarketsandMarkets 4Sodium Ferrocyanide Market: Analysis for Global & China Regions Development Trends Available at DeepResearchReports.com 2Sodium Ferrocyanide Market: Analysis for Global & China Regions Development Trends Available at DeepResearchReports.com 3Cytori Therapeutics to Provide Corporate Update and Investor Presentation on December 4 through a Live, Interactive Webcast 2Cytori Therapeutics to Provide Corporate Update and Investor Presentation on December 4 through a Live, Interactive Webcast 3Cytori Therapeutics to Provide Corporate Update and Investor Presentation on December 4 through a Live, Interactive Webcast 4
... /PRNewswire-FirstCall/ - Oncothyreon Inc. (Nasdaq: ONTY ... Eastland as Chief Financial Officer and Vice President, ... responsibilities will include financial management, investor relations and ... than 25 years of financial management experience to ...
... -- Chemists at Indiana University Bloomington have designed a molecule ... to release the ions in the presence of ultraviolet light. ... Society today (online), IU Bloomington chemist Amar Flood and ... how it works and, just as importantly, how they know ...
... Innovations (MBI) will be showcasing Zequanox ,™ ... at the International Conference on Aquatic Invasive Species ... September 2, 2010.  The conference is attended by participants ... and other stakeholders involved in the issues of aquatic ...
Cached Biology Technology:ONCOTHYREON APPOINTS JULIE M. EASTLAND AS CHIEF FINANCIAL OFFICER 2IU chemists develop new 'light switch' chloride binder 2IU chemists develop new 'light switch' chloride binder 3Marrone Bio Innovations to Present Invasive Mussel Control Solution 2
(Date:11/21/2014)... YORK , Nov. 19, 2014  Earlier this ... at New York College, and one of the most ... biosensor signals that are transmitted from Smartphones to third ... were Smartphones and has one of the earliest known ... discussing its usage in the military, child care, elder ...
(Date:11/18/2014)... 2014   News Highlights: ... develop The Partners Data Lake, an agile data ... Lake will allow researcher and clinicians to explore ... the lives of patients , The Partners ... across the Partners system, breaking down physical barriers ...
(Date:11/11/2014)... -- Forensicon, Inc., a Chicago -based digital ... promotion of Yaniv Schiff from Senior Computer ... new role as Director, he will lead company efforts ... and provide leadership within the company,s digital forensics practice ... Schiff joined Forensicon in 2006 and ...
Breaking Biology News(10 mins):Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 2Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 3Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 4Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 5Yaniv Schiff Promoted to Director of Digital Forensics at Forensicon, Inc. 2
... The genome of Medicago , a close relative of alfalfa ... been sequenced by an international team of scientists, capturing around 94 ... insights into the evolution of the Papilionoid subfamily of ... Plants in this family can house and work with ...
... SALT LAKE CITY While previous studies have linked ... disease, new research at the Intermountain Medical Center Heart ... to the onset of a dangerous heart condition known ... the flagship facility for the Intermountain Healthcare system, studied ...
... Ditching the diet for Thanksgiving? Turkey with all ... to miss their goals, according to a new Cornell ... subconsciously bending willpower every day. "We,re slaves to ... and of psychology at Cornell, who co-authored the article ...
Cached Biology News:Genome sequence sheds new light on how plants evolved nitrogen-fixing symbioses 2New study links excessive amounts of vitamin D to onset of atrial fibrillation 2New study links excessive amounts of vitamin D to onset of atrial fibrillation 3
... comprehensive microarray service for systematic discovery ... non-coding RNAs (ncRNAs) using a novel ... which enables highly sensitive and specific ... This comprehensive service includes custom probe ...
... comprehensive kinase analysis service utilizing high ... microarrays synthesized on Paraflo microfluidic chips ... measurement of kinase kinetic activities, and ... includes preparation of your kinase sample, ...
... a unique new tool, OligoMix for low ... needs in genomics, proteomics, synthetic biology, drug ... MIXTURE of a large number of DESIGNED ... single microtube. OligoMix is made from an ...
... PF 2D is an automated, two-dimensional fractionation ... mixtures. Part of Beckman Coulters ProteomeLab ... results in just hours. The system ... in cell lysates, addressing a key challenge ...
Biology Products: