Isolate nuclear and cytoplasmic RNA and protein from cultured cells
No phenol extraction or alcohol precipitation
Ambion's new PARIS Kit uses a fast, simple procedure based on Ambion's RNAqueous technology to isolate both RNA and native protein from the same experimental sample. The kit also permits separation of nuclear and cytoplasmic fractions prior to RNA and/or protein isolation. The resulting protein and RNA samples are suitable for various downstream applications, reducing time, cost, and variability between independent experimental samples.
Protein And RNA Isolation System (PARIS)
Isolation of high quality RNA is the first step for a variety of gene expression analyses. Very often complementary studies at the protein level are also required, e.g. performing further in vitro functional assays, analyzing a regulatory pathway, or simply correlating RNA and protein expression levels. Usually these analyses are performed using different aliquots of the same experimental sample. However, when working with rare, difficult to obtain, or very small samples it is sometimes impractical to isolate RNA and native proteins independently. In studies involving large numbers of samples, expensive reagents, or inherent variability (e.g. cell transfection), the addition of independent experimental samples is not only costly and time consuming, but may also lead to inconsistent results. To solve these issues Ambion scientists have developed a unique Protein And RNA Isolation System (PARIS) that allows researchers to isolate both RNA and protein from t