Navigation Links
New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting

ABSTRACT

New Brunswick Scientific has introduced a new device that automatically counts yeast cells in about 30 seconds, making manual counting obsolete. Like the original NuceloCounter, an automated device for counting mammalian cells, the new NucleoCounter YC-100 for yeast combines a fluorescence microscope with CCD camera and integrated image analysis software to perform rapid and accurate cell counts.

Monitoring the cell count during a fermentation run allows the scientist to activate inducible systems, or harvest at optimal times for the best product yield. The NucleoCounter YC- 100 can provide a fast and accurate DNA-based volumetric cell count within seconds, independent of debris or substrate aggregates in the media.

This system uses a disposable sampling device, the NucleoCassette. The small, plastic cassette is internally pre-coated with a fluorescent dye, propidium iodide (PI), which stains the cells' nuclei. Because the NucleoCounter detects signals from the stained nuclei, and not the cell, the system operates without regard to cell size or morphology.

MATERIALS AND METHODS

Withdraw 100 μL of culture to ensure a representative sample, and place 50 μL into a 1.5 mL tube. Mix thoroughly by inverting and shaking to ensure homogeneity. Treat with 450 μL of Reagent YC-100, a lysis/disaggregation buffer, which permeates the yeast cell wall and membranes, allowing the cellular DNA to be stained. Reagent YC-100 has the ability to dissociate minor clusters of yeast cells, but not to dissolve or dissociate cells that are in the process of reproductive division. Vortex or pipette vigorously for a few seconds to mix. The total volume is now 500 μL, with a 10-fold dilution. If necessary, repeat the dilution process to yield a sample 1 x 105 cells/mL to 2 x 106 cells/mL. (In gene
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Purified Single-Stranded M13 DNA for Automated Sequencing
2. Automated Fluorescent-Tag Cycle Sequencing
3. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
4. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
5. Agilent 2100 Bioanalyzer Automated Analysis System
6. Automated RNA Amplification for Arrays
7. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
8. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
9. Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents
10. DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging
11. Small-Molecule Quantitation Using Automated Single-Shot Averaging MALDI-TOF Mass Spectrometry
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Automated Yeast Cell Counter Ends Tedium Errors Manual Counting

(Date:3/26/2015)... SQI Diagnostics Inc. ("SQI" or the "Company") (TSX-V: SQD; ... including the appointment of auditors, its annual and special ... "Meeting") was adjourned, as previously disclosed on March 25, ... Toronto time). The date of the ... The Meeting will reconvene at the offices of the ...
(Date:3/26/2015)... Corporation (NASDAQ: NEOG ) announced today that its ... which ended Feb. 28, increased 13% to $7,454,000, when ... in the current quarter were $0.20, compared to $0.18 ... over prior year to $24,142,000, or $0.65 per share, ... same period a year ago. Revenues for ...
(Date:3/26/2015)... Ale Gicqueau, Founder and President of ... Affairs at Align Technology, on “ Conducting Postmarket Registries ... 2015 Global Conference and Exhibition on April 25th through ... During the poster presentation, Ale Gicqueau and Victor Chen ... Postmarket Registry, the requirements and resources to conduct the ...
(Date:3/25/2015)... , March 25, 2015 /PRNewswire/ - SQI Diagnostics Inc. ... that, as a result of recent discussions with certain ... shares of the Company, after dealing with certain matters ... special meeting of shareholders on March 26, 2015 (the ... the election of directors will take place when the ...
Breaking Biology Technology:SQI Diagnostics Inc. Announces Adjournment of Meeting 2Neogen reports 13% increase in net income 2Neogen reports 13% increase in net income 3Neogen reports 13% increase in net income 4Neogen reports 13% increase in net income 5Neogen reports 13% increase in net income 6Neogen reports 13% increase in net income 7Neogen reports 13% increase in net income 8Clinovo Presents at ACRP 2015 in Salt Lake City, Utah on April 25th-28th 2Clinovo Presents at ACRP 2015 in Salt Lake City, Utah on April 25th-28th 3SQI Diagnostics Inc. Announces New Nominees to SQI Diagnostics Board and Adjournment of Meeting 2SQI Diagnostics Inc. Announces New Nominees to SQI Diagnostics Board and Adjournment of Meeting 3SQI Diagnostics Inc. Announces New Nominees to SQI Diagnostics Board and Adjournment of Meeting 4SQI Diagnostics Inc. Announces New Nominees to SQI Diagnostics Board and Adjournment of Meeting 5
... ... ... , , , ... ...
... ... web to cost effectively boost the pool of eligible patients for screening for any ... upload recruitment material approved by their Institutional Review Board (IRB), on their own specific ... ...
... ... ... ... ...
Cached Biology Technology:Spectral granted IDE approval by U.S. FDA to conduct pivotal trial for Toraymyxin(TM) 2Spectral granted IDE approval by U.S. FDA to conduct pivotal trial for Toraymyxin(TM) 3Spectral granted IDE approval by U.S. FDA to conduct pivotal trial for Toraymyxin(TM) 4RESolutions Rapid Enrollment Solutions, LLC Announces Launch of Novel Patient Recruitment Web Site - Clinical Trial Spotlight™ 2RESolutions Rapid Enrollment Solutions, LLC Announces Launch of Novel Patient Recruitment Web Site - Clinical Trial Spotlight™ 3China-Biotics, Inc. Inaugurated the Commercial Production of its New Production Facility 2China-Biotics, Inc. Inaugurated the Commercial Production of its New Production Facility 3China-Biotics, Inc. Inaugurated the Commercial Production of its New Production Facility 4China-Biotics, Inc. Inaugurated the Commercial Production of its New Production Facility 5China-Biotics, Inc. Inaugurated the Commercial Production of its New Production Facility 6China-Biotics, Inc. Inaugurated the Commercial Production of its New Production Facility 7
(Date:3/12/2015)... March 12, 2015 IriTech, a leading iris ... of the Texas Instruments Design Network, announced today that ... demonstrated its IriShield USB MK2120U device during a launching ... pension distribution. The iris scanner is ... marketed in India through its ...
(Date:3/12/2015)... , March 12, 2015 ... standardized identity and access management (IAM) solution for ... schedule. With Beta System,s new IAM package, customers ... practices gleaned from multiple IAM implementations across different ... software as well as any necessary services and ...
(Date:3/11/2015)... and Markets ( http://www.researchandmarkets.com/research/3p32qm/access_control ) has announced ... Product, Application & By Geography - Global Forecast & ... This report predicts that the access control ... with an estimated CAGR of 10.6%. The ... as contact cards & readers, contactless cards & readers, ...
Breaking Biology News(10 mins):Indian States See IriTech's Scanner Bolstering Efficiency of Authentication for Aadhaar 2Indian States See IriTech's Scanner Bolstering Efficiency of Authentication for Aadhaar 3Beta Systems Fast Forward IAM Available Now: a Standardized IAM Package at a Fixed Price 2Global Access Control (Cards & Readers, Biometrics, Controllers, Management Software) Market 2015-2020 - Analysis of the $10 Billion Market 2
... , Scientists from the University of Granada and ... out that some critical patients could improve their oxide stress ... antioxidant treatment through food. This study has proved that the ... Unit (ICU), due to the low levels of antioxidant food ...
... New Hampshire researchers have received a significant grant ... a sustainable closed agroecosystem, exploring viable strategies for ... the U.S. Department of Agriculture,s Sustainable Agricultural Research ... closing energy and nutrient cycles could help small ...
... Conn.Yale Environment 360, a new online magazine dedicated to ... http://e360.yale.edu . Edited by Roger Cohn, the former editor ... aims to become one of the leading Web sites ... of the day. The magazine,s inaugural edition features ...
Cached Biology News:A nutritional supplement could improve the clinical situation of ICU patients 2UNH receives $380,000 grant to study organic dairy as closed ecosystem 2UNH receives $380,000 grant to study organic dairy as closed ecosystem 3Yale Environment 360 magazine launches online 2
... detects an ~185 kDa protein, corresponding to ... at tyrosine 1248 on SDS-PAGE immunoblots. The ... against the non-phosphorylated peptide to remove antibodies ... the protein. In cell lines where ...
Mouse monoclonal [4A2.2] to SLC6A4 ( Abpromise for all tested applications). entrezGeneID: 6532 SwissProtID: P31645...
...
... kit provides all the accessories necessary for ... conformation polymorphism analysis (SSCP) with the DCode ... a cooling finger adaptor for use with ... of 20 cm plates, 2 sets of ...
Biology Products: