Navigation Links
New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting

ABSTRACT

New Brunswick Scientific has introduced a new device that automatically counts yeast cells in about 30 seconds, making manual counting obsolete. Like the original NuceloCounter, an automated device for counting mammalian cells, the new NucleoCounter YC-100 for yeast combines a fluorescence microscope with CCD camera and integrated image analysis software to perform rapid and accurate cell counts.

Monitoring the cell count during a fermentation run allows the scientist to activate inducible systems, or harvest at optimal times for the best product yield. The NucleoCounter YC- 100 can provide a fast and accurate DNA-based volumetric cell count within seconds, independent of debris or substrate aggregates in the media.

This system uses a disposable sampling device, the NucleoCassette. The small, plastic cassette is internally pre-coated with a fluorescent dye, propidium iodide (PI), which stains the cells' nuclei. Because the NucleoCounter detects signals from the stained nuclei, and not the cell, the system operates without regard to cell size or morphology.

MATERIALS AND METHODS

Withdraw 100 μL of culture to ensure a representative sample, and place 50 μL into a 1.5 mL tube. Mix thoroughly by inverting and shaking to ensure homogeneity. Treat with 450 μL of Reagent YC-100, a lysis/disaggregation buffer, which permeates the yeast cell wall and membranes, allowing the cellular DNA to be stained. Reagent YC-100 has the ability to dissociate minor clusters of yeast cells, but not to dissolve or dissociate cells that are in the process of reproductive division. Vortex or pipette vigorously for a few seconds to mix. The total volume is now 500 μL, with a 10-fold dilution. If necessary, repeat the dilution process to yield a sample 1 x 105 cells/mL to 2 x 106 cells/mL. (In general, a 100 to 1,000-fold dilution is generally suitable.)

Load the sample into the NucleoCassette, using the built-in piston to aspirate a pre-defined volume of approximately 50 μL. Place the NucleoCassette into the NucleoCounter and press "Run". In about 30 seconds, the total cell count will be calculated and shown on a built-in display.

RESULTS AND DISCUSSION

Using optional NucleoView software connected to your PC, you may additionally store and export results, as well as view an image of the stained cells. Results may be plotted as Optical Density (OD600) against Cell concentration (cells/mL) or Dry Cellular Weight (g/L) against Cell concentration (cells/mL).

Figure 2: NucleoCounter YC-100 raw image of yeast cells in suspension ( Saccharomyces cerevisiae ) after Reagent YC-100 treatment using 100-fold dilution. Cell count is approx. 8 x 107/mL. The objects have similar fluorescence intensities and are uniform in size, indicating minimal cell disruption. The yeast cells are uniformly dispersed throughout the entire image. The yeast cells may be manually monitored in the NucleoCassette measurement chamber using fluorescence microscopy with appropriate filters for propidium iodide.

Clean up is simple. After use, the NucleoCassette can be disposed of as biological waste, with the PI dye safely enclosed. Unlike flow systems and hemacytometers, the system requires no cleaning, maintenance, or calibration. For in-lab demo, contact New Brunswick Scientific. For additional information contact Mike Sattan, Director of Marketing via email at sattan@nbsc.com


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Purified Single-Stranded M13 DNA for Automated Sequencing
2. Automated Fluorescent-Tag Cycle Sequencing
3. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
4. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
5. Agilent 2100 Bioanalyzer Automated Analysis System
6. Automated RNA Amplification for Arrays
7. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
8. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
9. Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents
10. DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging
11. Small-Molecule Quantitation Using Automated Single-Shot Averaging MALDI-TOF Mass Spectrometry
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2016)... June, 23, 2016  The Biodesign Challenge (BDC), a ... ways to harness living systems and biotechnology, announced its ... in New York City . ... students, showcased projects at MoMA,s Celeste Bartos Theater during ... , MoMA,s senior curator of architecture and design, and ...
(Date:6/23/2016)... , June 23, 2016 Apellis Pharmaceuticals, ... 1 clinical trials of its complement C3 inhibitor, ... and multiple ascending dose studies designed to assess ... of subcutaneous injection in healthy adult volunteers. ... either as a single dose (ranging from 45 ...
(Date:6/23/2016)... , ... June 23, 2016 , ... ... quality, regulatory and technical consulting, provides a free webinar on Performing ... July 13, 2016 at 12pm CT at no charge. , Incomplete investigations are ...
(Date:6/22/2016)... June 22, 2016 Research and Markets has ... report to their offering. ... 2014 from $29.3 billion in 2013. The market is expected to ... from 2015 to 2020, increasing from $50.6 billion in 2015 to ... forecasts during the forecast period (2015 to 2020) are discussed. As ...
Breaking Biology Technology:
(Date:6/15/2016)... 2016 Transparency Market Research ... Market by Application Market - Global Industry Analysis Size Share ... the report, the  global gesture recognition market  was ... is estimated to grow at a CAGR of ... Increasing application of gesture recognition technology ...
(Date:6/2/2016)... LONDON , June 2, 2016 ... has awarded the 44 million US Dollar project, ... Security Embossed Vehicle Plates including Personalization, Enrolment, and IT Infrastructure ... world leader in the production and implementation of Identity Management ... in January, however Decatur was selected ...
(Date:5/20/2016)... -- VoiceIt is excited to announce its new marketing ... working together, VoiceIt and VoicePass will offer an ... slightly different approaches to voice biometrics, collaboration between ... Both companies ... "This marketing and technology partnership allows VoiceIt ...
Breaking Biology News(10 mins):