Navigation Links
New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting

ABSTRACT

New Brunswick Scientific has introduced a new device that automatically counts yeast cells in about 30 seconds, making manual counting obsolete. Like the original NuceloCounter, an automated device for counting mammalian cells, the new NucleoCounter YC-100 for yeast combines a fluorescence microscope with CCD camera and integrated image analysis software to perform rapid and accurate cell counts.

Monitoring the cell count during a fermentation run allows the scientist to activate inducible systems, or harvest at optimal times for the best product yield. The NucleoCounter YC- 100 can provide a fast and accurate DNA-based volumetric cell count within seconds, independent of debris or substrate aggregates in the media.

This system uses a disposable sampling device, the NucleoCassette. The small, plastic cassette is internally pre-coated with a fluorescent dye, propidium iodide (PI), which stains the cells' nuclei. Because the NucleoCounter detects signals from the stained nuclei, and not the cell, the system operates without regard to cell size or morphology.

MATERIALS AND METHODS

Withdraw 100 μL of culture to ensure a representative sample, and place 50 μL into a 1.5 mL tube. Mix thoroughly by inverting and shaking to ensure homogeneity. Treat with 450 μL of Reagent YC-100, a lysis/disaggregation buffer, which permeates the yeast cell wall and membranes, allowing the cellular DNA to be stained. Reagent YC-100 has the ability to dissociate minor clusters of yeast cells, but not to dissolve or dissociate cells that are in the process of reproductive division. Vortex or pipette vigorously for a few seconds to mix. The total volume is now 500 μL, with a 10-fold dilution. If necessary, repeat the dilution process to yield a sample 1 x 105 cells/mL to 2 x 106 cells/mL. (In general, a 100 to 1,000-fold dilution is generally suitable.)

Load the sample into the NucleoCassette, using the built-in piston to aspirate a pre-defined volume of approximately 50 μL. Place the NucleoCassette into the NucleoCounter and press "Run". In about 30 seconds, the total cell count will be calculated and shown on a built-in display.

RESULTS AND DISCUSSION

Using optional NucleoView software connected to your PC, you may additionally store and export results, as well as view an image of the stained cells. Results may be plotted as Optical Density (OD600) against Cell concentration (cells/mL) or Dry Cellular Weight (g/L) against Cell concentration (cells/mL).

Figure 2: NucleoCounter YC-100 raw image of yeast cells in suspension ( Saccharomyces cerevisiae ) after Reagent YC-100 treatment using 100-fold dilution. Cell count is approx. 8 x 107/mL. The objects have similar fluorescence intensities and are uniform in size, indicating minimal cell disruption. The yeast cells are uniformly dispersed throughout the entire image. The yeast cells may be manually monitored in the NucleoCassette measurement chamber using fluorescence microscopy with appropriate filters for propidium iodide.

Clean up is simple. After use, the NucleoCassette can be disposed of as biological waste, with the PI dye safely enclosed. Unlike flow systems and hemacytometers, the system requires no cleaning, maintenance, or calibration. For in-lab demo, contact New Brunswick Scientific. For additional information contact Mike Sattan, Director of Marketing via email at sattan@nbsc.com


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Purified Single-Stranded M13 DNA for Automated Sequencing
2. Automated Fluorescent-Tag Cycle Sequencing
3. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
4. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
5. Agilent 2100 Bioanalyzer Automated Analysis System
6. Automated RNA Amplification for Arrays
7. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
8. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
9. Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents
10. DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging
11. Small-Molecule Quantitation Using Automated Single-Shot Averaging MALDI-TOF Mass Spectrometry
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:1/21/2017)... Jan. 20, 2017 Interpace Diagnostics Group, ... that provides clinically useful molecular diagnostic tests and ... into a securities purchase agreement with three  institutional ... of common stock in a registered direct offering.  ... agreed to sell to the same investors warrants ...
(Date:1/20/2017)... ... ... The two newest companies to join the University City Science Center’s Port business ... Wistar Institute, and Sanguis, launched by a trio of students from the University of ... developing a treatment for a chronic viral infection and its associated diseases, with the ...
(Date:1/20/2017)... MA (PRWEB) , ... January ... ... leader in Less Exposure Surgery (LES®) Technologies, announced today the next evolution ... PedFuse Pedicle Screw System platform). In contrast to the competition, SpineFrontier is ...
(Date:1/19/2017)... 19, 2017 /PRNewswire -- WuXi AppTec, a leading ... and technology platform, today announced that it has ... preclinical drug discovery contract research organization (CRO). After ... wholly-owned subsidiary of WuXi, and will continue to ... greater services. The acquisition will further strengthen WuXi,s ...
Breaking Biology Technology:
(Date:1/13/2017)... Jan. 13, 2017 Sandata Technologies, LLC, ... the homecare industry, including Electronic Visit Verification™ (EVV™), ... Justin Jugs, as Senior Vice President of Product ... years of homecare experience to Sandata, where he ... plans to align Sandata,s suite of solutions with ...
(Date:1/11/2017)... Jan. 11, 2017 Intoxalock, a leading ignition ... release of its patent-pending calibration device. With this new ... calibrations, securely upload data logs and process repairs at ... "Fighting drunk driving through the application of ... at large, but also for the customer who can ...
(Date:1/6/2017)... 5, 2017  Delta ID Inc., a leader in ... for automotive at CES® 2017. Delta ID has collaborated ... demonstrate the use of iris scanning as a secure, ... driver in a car, and as a way to ... Delta ID and Gentex will demonstrate (booth ...
Breaking Biology News(10 mins):