Navigation Links
Tenfold boost in ability to pinpoint proteins in cancer cells
Date:3/19/2013

same tissue sample, testing for biomarkers in groups of 10 in each round.

"Proteins are the building blocks for cell function and cell behavior, but their makeup in a cell is highly complex," Gao said. "You need to look at a number of indicators (biomarkers) to know what's going on."

The new process works like this: Gao and his team purchase antibodies that are known to bind with the specific biomarkers they want to test for in a cell. They pair quantum dots with the antibodies in a fluid solution, injecting it onto a tissue sample. Then, they use a microscope to look for the presence of fluorescent colors in the cell. If they see particular quantum dot colors in the tissue sample, they know the corresponding biomarker is present in the cell.

After completing one cycle, Gao and co-author Pavel Zrazhevskiy, a UW doctoral student in bioengineering, inject a low-pH fluid into the cell tissue that neutralizes the color fluorescence, essentially wiping the sample clean for the next round. Remarkably, the tissue sample doesn't degrade at all even after 10 such cycles, Gao said.

For cancer research and treatment, in particular, it's important to be able to look at a single cell at high resolution to examine its details. For example, if 99 percent of cancer cells in a person's body respond to a treatment drug, but 1 percent doesn't, it's important to analyze and understand the molecular makeup of that 1 percent that responds differently.

"When you treat with promising drugs, there are still a few cells that usually don't respond to treatment," said Gao. "They look the same, but you don't have a tool to look at their protein building blocks. This will really help us develop new drugs and treatment approaches."

The process is relatively low-cost and simple, and Gao hopes the procedure can be automated. He envisions a chamber to hold the tissue sample, and wire-thin pumps to inject and vacuum out fluid between cycles. A microsc
'/>"/>

Contact: Michelle Ma
mcma@uw.edu
206-543-2580
University of Washington
Source:Eurekalert  

Page: 1 2 3

Related biology technology :

1. New semiconductor research may extend integrated circuit battery life tenfold
2. Are Pros "Cheating" with Undetectable HGH Boosters?
3. Genuine Bio-Fuel Inc. Notes Sustainable Energy Gets A Much Needed Boost from the President Obama During 2013 Inaugural Address
4. Genedata Biologics 3.0 Boosts Efficiency Through Standardization
5. Tiny structure gives big boost to solar power
6. Nature Materials study: Boosting heat transfer with nanoglue
7. Industrial carbon management research gets $3.75 million boost
8. A 300 Million Euro Boost for Biomedicine in Berlin
9. Rice team boosts silicon-based batteries
10. LLSs Lead Therapy Acceleration Program in MDS Gets Boost from Major Industry Licensing Agreement
11. Spinach power gets a big boost
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Tenfold boost in ability to pinpoint proteins in cancer cells
(Date:10/22/2014)... , Oct. 22, 2014 /PRNewswire/ - iCo Therapeutics ("iCo" ... announced next steps for its Oral Amphotericin B ... its in vitro work involving samples ... now plans to complete pre clinical studies and ... Phase 1A clinical trial, utilizing approximately $700,000 of ...
(Date:10/20/2014)... 20, 2014 The report “Pharmacy ... Systems, Packaging and Labeling Systems, Table-top Counters) by ... Global Forecasts & Trends to 2019” analyzes and ... in North America, Europe, Asia-Pacific, and the Rest ... tables and 30 figures spread through 300 pages ...
(Date:10/20/2014)... 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or "the Company") ... orders to $5.8 million and provides a good start to Q4.  ... North America and one in the Middle ... at record levels," said Peter Bruijns , President & CEO. ... of Q3 than they have been for any complete year since ...
(Date:10/20/2014)... OncLive® is pleased ... at Thomas Jefferson University has joined its Strategic ... Alliance Partnership program, the Sidney Kimmel Cancer Center ... awareness of the Center’s cutting-edge research programs, comprehensive ... and other health care professionals from the Sidney ...
Breaking Biology Technology:iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 2iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 3Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 2Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 3Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 4Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 2Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 3
... from Product Sales Increased 149% to $3.5 Million - ... - Gross Profit ... AMDL, Inc. (Amex: ADL ),is a vertically integrated ... Jiangxi and Jilin,China. Through its wholly owned subsidiary Jade Pharmaceutical ...
... scatter and channel light, University of California, San ... junction solar cells with the potential for nearly ... Diego effort to break the theoretical limit of ... recently received a big funding boost from the ...
... 15 /PRNewswire-FirstCall/ - BioMS Medical Corp. (TSX:,MS), a leading ... financial and operational results for the first quarter ended,March ... Company, is,developing dirucotide (also known as MBP8298), a synthetic ... in Canada, Europe and the US., "During the ...
Cached Biology Technology:AMDL Announces First Quarter 2008 Results Posting Sales of $3.58 Million 2AMDL Announces First Quarter 2008 Results Posting Sales of $3.58 Million 3UC San Diego nanostructures will raise thin-film solar cell efficiency 2BioMS Medical announces first quarter 2008 results 2BioMS Medical announces first quarter 2008 results 3BioMS Medical announces first quarter 2008 results 4BioMS Medical announces first quarter 2008 results 5
(Date:10/16/2014)... can be cultivated efficiently, they are anything but ... by monoculture cultivation is becoming increasingly evident. Despite ... form and are regarded as the sole possibility ... quite wrongfully, finds Bernhard Schmid, an ecology professor ... novel form of agriculture and forestry. After all, ...
(Date:10/15/2014)... PORT WASHINGTON, N.Y. , Oct. 15, 2014 ... in technology solutions for home and community-based care, ... the benefits of implementing Sandata,s Santrax® Electronic Visit ... Quality Care Services is a home health company ... Texas . ...
(Date:10/15/2014)... spreading rapidly and to an unexpected extent. The outbreak ... and the virus shows a new disease dynamic in ... this reason, the German National Academy of Sciences Leopoldina, ... and the Union of the German Academies of Sciences ... epidemic today. , In the statement the academies call ...
Breaking Biology News(10 mins):Plant communities produce greater yield than monocultures 2Plant communities produce greater yield than monocultures 3Sandata Announces Case Study with Quality Care Services, Inc. 2Academies call for consequences from the Ebola virus epidemic 2Academies call for consequences from the Ebola virus epidemic 3Academies call for consequences from the Ebola virus epidemic 4Academies call for consequences from the Ebola virus epidemic 5
... PA, January 15, 2013 A new study has ... counteract some pathologic features in a mouse model of ... known to produce long-term improvement of the symptoms in ... characterized by progressive muscle wasting, respiratory and cardiac impairments, ...
... should sharpen their ability to spot environmental conditions that ... having a certain abundance of fish assures how much ... harvest of fish is only closely linked to abundance ... a University of Washington-led study, according to Ray Hilborn, ...
... A research team involving several scientists from the University ... in the devastating pine beetle outbreaks ravaging the West: ... streams as scientists had previously believed. According to ... shows that smaller trees and other vegetation that survive ...
Cached Biology News:Tamoxifen ameliorates symptoms of Duchenne muscular dystrophy 2Potential harvest of most fish stocks largely unrelated to abundance 2Potential harvest of most fish stocks largely unrelated to abundance 3CU-led study shows pine beetle outbreak buffers watersheds from nitrate pollution 2CU-led study shows pine beetle outbreak buffers watersheds from nitrate pollution 3
Request Info...
Request Info...
... Art GLP facility with capabilities from ... fermentation. Coordinated process development from ... operation capable of simultaneous production runs ... Hosts include: Bacteria, Yeast, Fungi, ...
ExonBio Service provides one-stop recobinant protein shopping from cloning, expression and purification. Price including gene cloning....
Biology Products: