Navigation Links
Scientists target bacterial transfer of resistance genes
Date:10/25/2012

CHAMPAIGN, Ill. The bacterium Streptococcus pneumoniae which can cause pneumonia, meningitis, bacteremia and sepsis likes to share its antibiotic-defeating weaponry with its neighbors. Individual cells can pass resistance genes to one another through a process called horizontal gene transfer, or by "transformation," the uptake of DNA from the environment.

Now researchers report that they can interrupt the cascade of cellular events that allows S. pneumoniae to swap or suck up DNA. The new findings, reported in the journal PLoS ONE, advance the effort to develop a reliable method for shutting down the spread of drug resistance in bacteria.

"Within the last few decades, S. pneumoniae has developed resistance to several classes of antibiotics," said University of Illinois pathobiology professor Gee Lau, who led the study. "Importantly, it has been shown that antibiotic stress the use of antibiotics to treat an infection can actually induce the transfer of resistance genes among S. pneumoniae. Our approach inhibits resistance gene transfer in all strains of S. pneumoniae, and does so without increasing selective pressure and without increasing the likelihood that resistant strains will become dominant."

Lau and his colleagues focused on blocking a protein that, when it binds to a receptor in the bacterial cell membrane, spurs a series of events in the cell that makes the bacterium "competent" to receive new genetic material. The researchers hypothesized that interfering with this protein (called CSP) would hinder its ability to promote gene transfer.

In previous work published late last year in the journal PLoS Pathogens, Lau's team identified proteins that could be made in the lab that were structurally very similar to the CSP proteins. These artificial CSPs can dock with the membrane receptors, block the bacterial CSPs' access to the receptors and reduce bacterial competence, as well as reducing the infectious capacity of S. pneumoniae.

In the new study, the researchers fine-tuned the amino acid structure of more than a dozen artificial CSPs and tested how well they inhibited the S. pneumoniae CSPs. They also tested their ability (or, more desirably, their inability) to mimic the activity of CSPs in bacterial cells.

"The chemical properties of individual amino acids in a protein can greatly influence the protein's activity," Lau said.

The team identified several artificial CSPs that both inhibited the bacterial CSPs and reduced S. pneumoniae competence by more than 90 percent.

"This strategy will likely help us reduce the spread of antibiotic-resistance genes among S. pneumoniae and perhaps other species of streptococcus bacteria," Lau said.


'/>"/>
Contact: Diana Yates
diya@illinois.edu
217-333-5802
University of Illinois at Urbana-Champaign
Source:Eurekalert  

Related biology technology :

1. Scientists defuse the Vietnam time bomb
2. Singapore scientists lead human embryonic stem cell study
3. Sheffield scientists shine a light on the detection of bacterial infection
4. Nanowiggles: Scientists discover graphene nanomaterials with tunable functionality in electronics
5. Scientists solve mystery of colorful armchair nanotubes
6. iBioSeminars and iBioMagazine: Free, Online Biology Seminars and Short Talks by Leading Scientists
7. Chinese Scientists Zhen-Yi Wang and Zhu Chen Awarded 7th Annual Szent-Gyorgyi Prize for Progress in Cancer Research
8. Scientists decode brain waves to eavesdrop on what we hear
9. Receptos Scientists Publish Determination of a High Resolution Sphingosine 1-Phosphate Receptor 1 Structure in Science
10. Scientists learn how to out run damage with imaging technique
11. Design eye for the science guy: Drop-in clinic helps scientists communicate data
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Scientists target bacterial transfer of resistance genes
(Date:12/8/2016)... ... December 08, 2016 , ... Lajollacooks4u, San Diego’s premier team building ... team building events, new program offerings and company expansion. , This is ... to include groups of over 30 people. Ever since, Lajollacooks4u has seen significant demand ...
(Date:12/8/2016)... ... December 08, 2016 , ... ... of innovation is taking over sports. On Thursday, December 15th a panel of ... is disrupting the playing field at a Smart Talk session. Smart Talk will ...
(Date:12/8/2016)... , Dec. 8, 2016  Biotheranostics today announced ... role of the Breast Cancer Index (BCI) in ... cancer are most at-risk for disease recurrence and ... results from three studies advancing the understanding of ... to tumor biology and inform decisions related to ...
(Date:12/8/2016)... Iowa , Dec. 8, 2016 Eurofins announces the ... Food and President of Eurofins Scientific Inc. (ESI). ... with his proven professional and entrepreneurial experience in leading international business ... the US food testing market to uphold Eurofins, status as the ... ...
Breaking Biology Technology:
(Date:12/7/2016)... Avanade is helping Williams Martini Racing, one of ... biometric data in order to critically analyse every aspect ... against their rivals after their impressive, record-breaking pit stop ... with Williams during the 2016 season to capture and ... rate, temperature and peak acceleration) for key members of ...
(Date:12/5/2016)... WASHINGTON , Dec. 5, 2016  The ... (NIJ), today published "Can CT Scans Enhance or ... examines the potential of supporting or replacing forensic ... a CT scan. In response to ... NIJ is exploring using CT scans as a ...
(Date:11/29/2016)... , Nov. 29, 2016 BioDirection, a ... point-of-care products for the objective detection of concussion and ... company has successfully completed a meeting with the U.S. ... Tbit™ blood test Pre-Submission Package. During the meeting company ... system as a precursor to commencement of a planned ...
Breaking Biology News(10 mins):