Navigation Links
Scientists learn how to 'out run damage' with imaging technique
Date:2/21/2012

VANCOUVER, CANADA - Over the decades X-ray crystallography has been fundamental in the development of many scientific fields. The method has revealed the structure and function of many biological molecules, including vitamins, drugs, proteins and nucleic acids such as DNA. However, in order to obtain good data, large single crystals are required. These are often nearly impossible to grow. There also is the problem that X-rays damage delicate biological samples.

"From the beginning, the resolution of images recorded by biologists has been limited by damage due to the radiation used," said physicist John C. H. Spence, a Regents' Professor in physics at Arizona State University. "But what happens if a pulse of imaging radiation is used that terminates before damage begins, yet contains sufficient photons to generate a useful scattering pattern?"

Indeed, results of such a method are being reported by Spence at the American Association for the Advancement of Science annual meeting in Vancouver, Canada. Spence presented his findings today (Feb. 17) during a special session on "Imaging and Controlling Molecular Dynamics with Ultrashort Laser Pulses."

Many in the scientific community didn't believe such a method could work. Yet, said Spence "The experiments of Henry Chapman's (University of California, Davis) group using lithographed structures and soft (i.e. long wavelength) X-rays had shown that if we could 'out-run the damage,' this might indeed be a useful path to damage-free imaging at atomic resolution. In my lab we were thinking about the data analysis, and building the hydrated protein-beam injector device, a bit like an ink-jet printer, to spray the molecules across an X-ray laser. This snap-shot method should eventually allow us to make movies of molecular machines at work."

Spence joined forces with Chapman and many collaborators to recently demonstrate serial snapshot femtosecond (10-15 of a second) diffraction (SFX) from nanocrystals using the world's first hard X-ray laser. The photosystem I (PSI) nanocrystals came from Professor Petra Fromme's lab in ASU's Department of Chemistry and Biochemistry.

"These are early days for femtosecond diffractive imaging," noted Spence, who provided the theory and much of the data analysis. "But first indications are that high-resolution data can now be obtained at the nanoscale by this method. If we can indeed 'outrun' the many radiation-damage processes in this way, it will open the way to future experiments on laser-excited samples, 3-D image reconstruction and a host of other experiments on fast imaging, all directed to the grand challenge of obtaining movies showing molecules at work."


'/>"/>
Contact: jenny green
jenny.green@asu.edu
480-965-1430
Arizona State University
Source:Eurekalert

Related biology technology :

1. Clemson scientists put a (nano) spring in their step
2. City of Hope Helps KGI Launch New Management Training Program for Scientists
3. University of Pennsylvania scientists move optical computing closer to reality
4. Scientists grow nanonets able to snare added energy transfer
5. The National Cancer Institute Joins the Global Community of Scientists Now Using BIOMARKERcenter From Thomson Reuters
6. Scientists peel away the mystery behind golds catalytic prowess
7. SACHEM Launches 2-D HPLC e-Learning Program : New e-Learning Program Teaches Scientists How to Better Analyze and Prove Product Purity Through Greater Sensitivity and Precision in Identification of Trace Components
8. Vermillion and Stanford Scientists Receive Best Research Award From the PAD Coalition
9. Brewing better beer: Scientists determine the genomic origins of lager yeasts
10. Tengion Scientists Publish Positive Preclinical Findings With Neo-Organ Demonstrating Long-term Durability and Growth With Skeletal Maturation
11. CU scientists create worlds thinnest balloon -- just one atom thick
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/25/2016)... ... 25, 2016 , ... Biohaven Pharmaceutical Holding Company Ltd. (Biohaven) ... company’s orphan drug designation request covering BHV-4157 for the treatment of Spinocerebellar Ataxia ... , Spinocerebellar ataxia is a rare, debilitating neurodegenerative disorder that is estimated ...
(Date:5/24/2016)... ... May 24, 2016 , ... ... identity. The new Media Cybernetics corporate branding reflects a results-driven revitalization for a ... image analysis. The re-branding components include a crisp, refreshed logo and a new ...
(Date:5/23/2016)... OXFORD, England , May 23, 2016 ... Wednesday, May 25 th at 10:15 a.m. ET before ... about the role genetically engineered mosquitos can play in controlling ... known carrier of the Zika virus.      (Logo: ... genetically engineered male mosquito with a self-limiting gene. Trials in ...
(Date:5/22/2016)... ... 22, 2016 , ... Doctors in Rome say micronutrients found in certain foods ... Surviving Mesothelioma has just posted an article on the new research. Click here ... Clinical Sciences and Translational Medicine evaluated more than 150 studies on polyphenols in cancer ...
Breaking Biology Technology:
(Date:5/20/2016)... 2016  VoiceIt is excited to announce its ... By working together, VoiceIt and VoicePass will ... VoicePass take slightly different approaches to voice biometrics, ... and usability. ... partnership. "This marketing and technology partnership ...
(Date:5/9/2016)... -- Elevay is currently known as the ... high net worth professionals seeking travel for work   ... there is still no substitute for a face-to-face meeting. ... deal with a firm handshake. This is why wealthy ... citizenship via investment programs like those offered by the ...
(Date:4/28/2016)... First quarter 2016:   , ... the first quarter of 2015 The gross margin was ... 18.8) and the operating margin was 40% (-13) Earnings ... flow from operations was SEK 249.9 M (21.2) , ... SEK 7,000-8,500 M. The operating margin for 2016 is ...
Breaking Biology News(10 mins):