Navigation Links
Salk scientists use Amazon Cloud to view molecular machinery in remarkable detail
Date:2/1/2013

low appears as a blurry spot. The challenge for biologists is that the molecules they want to see are often only a few tens of nanometers in size.

"You have no idea how many single molecules are distributed within that blurry spot, so essential features and ideas remain obscure to you," says Jennifer Lippincott-Schwartz, a Salk non-resident fellow and coauthor on the paper.

In the early 2000s, several techniques were developed to break through the Abbe Limit, launching the new field of super-resolution microscopy. Among them was a method developed by Lippincott-Schwartz and her colleagues called Photoactivated Localization Microscopy, or PALM.

PALM, and its sister techniques, work because mathematics can see what the eye cannot: within the blurry spot, there are concentrations of photons that form bright peaks, which represent single molecules. The downside to these approaches is that it can take several hours to several days to crunch all the numbers required just to produce one usable image.

"It's like taking a movie, then you go through some very complex math, so what you see is the end result of processing, which is extremely slow because there's so many parameters," Cang says. "When I first saw PALM, I was shocked by how good it was. I wanted to use it right away, but when I actually tried to use it, I found its usefulness was limited by computing speed."

Even using statistical shortcuts, processing these images was still so intense that a supercomputer was required to reduce the time to a practical level. "Calculating an area of 50 pixels can take nearly a full day on a state-of-the-art desktop computer," says Lippincott-Schwartz. "But what you'll have achieved is the difference between a guess and a definitive answer."

In their Nature Methods paper, the researchers offer other scientists the tools they need to use an easier alternative-the Amazon Elastic Compute Cloud (Amazon Elastic EC2), a service
'/>"/>

Contact: andy Hoang
ahoang@salk.edu
619-861-5811
Salk Institute
Source:Eurekalert  

Page: 1 2 3

Related biology technology :

1. Scientists defuse the Vietnam time bomb
2. Singapore scientists lead human embryonic stem cell study
3. Sheffield scientists shine a light on the detection of bacterial infection
4. Nanowiggles: Scientists discover graphene nanomaterials with tunable functionality in electronics
5. Scientists solve mystery of colorful armchair nanotubes
6. iBioSeminars and iBioMagazine: Free, Online Biology Seminars and Short Talks by Leading Scientists
7. Chinese Scientists Zhen-Yi Wang and Zhu Chen Awarded 7th Annual Szent-Gyorgyi Prize for Progress in Cancer Research
8. Scientists decode brain waves to eavesdrop on what we hear
9. Receptos Scientists Publish Determination of a High Resolution Sphingosine 1-Phosphate Receptor 1 Structure in Science
10. Scientists learn how to out run damage with imaging technique
11. Design eye for the science guy: Drop-in clinic helps scientists communicate data
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Salk scientists use Amazon Cloud to view molecular machinery in remarkable detail
(Date:7/31/2014)... BLOOMINGTON, Ind. -- Chemists at Indiana University Bloomington ... in bricks-and-mortar fashion, a development with potential value ... as field-effect transistors and photovoltaic cells. , Their ... 3D Crystalline Solids and 2D Self-Assembled Crystals," has ... a journal of the Royal Society of Chemistry. ...
(Date:7/31/2014)... Graphite Metallizing Corporation announces GRAPHALLOY® bearings ... are uniquely suited to prevent loss of lubrication that ... high temperatures . , Recently, a manufacturer of ... at Graphite Metallizing to overcome problems with movement of ... pans containing blocks of powder mixture are loaded onto ...
(Date:7/31/2014)... (PRWEB) July 31, 2014 CollabRx, Inc. ... inform therapeutic decision-making in oncology, today announced that it ... 2014 to discuss the Company's financial results for the ... update about the business in the current fiscal year. ... beginning on Thursday, August 14, 2014, at 5 p.m. ...
(Date:7/31/2014)... Francisco, Calif. (PRWEB) July 31, 2014 ... newly diagnosed patients with an overview of the disease ... management of drug side effects, as well as updates ... “We’re grateful to Dr. Sandy Srinivas for organizing this ... survivor of kidney cancer. “Sandy and her colleagues have ...
Breaking Biology Technology:Chemists demonstrate 'bricks-and-mortar' assembly of new molecular structures 2Chemists demonstrate 'bricks-and-mortar' assembly of new molecular structures 3Graphite Metallizing Announces Bearings for High Temperature Oven Applications 2Graphite Metallizing Announces Bearings for High Temperature Oven Applications 3CollabRx, Inc. Schedules First Quarter Fiscal Year 2015 Financial Conference Call and Business Update 2
... deCODE genetics,(Nasdaq: DCGN ) today announced its ... 2008. A conference call to discuss the,quarter,s results, ... webcast live tomorrow, Friday, November 7, at 8:00am ... third quarter 2008 was $11.3 million, compared,to $22.2 ...
... NGX-4010 for management of pain associated ... with PHN, ... review for NGX-4010 ... NGSX ), a biopharmaceutical company focused on developing and,commercializing novel pain management ...
... While Public Relations has,long been used as a ... ability to reach consumers has never been greater. ... the traditional press,release as effective and low-cost methods ... was conducted by Best Practices, LLC to,identify what ...
Cached Biology Technology:deCODE genetics Announces Third Quarter 2008 Financial Results 2deCODE genetics Announces Third Quarter 2008 Financial Results 3deCODE genetics Announces Third Quarter 2008 Financial Results 4deCODE genetics Announces Third Quarter 2008 Financial Results 5deCODE genetics Announces Third Quarter 2008 Financial Results 6deCODE genetics Announces Third Quarter 2008 Financial Results 7deCODE genetics Announces Third Quarter 2008 Financial Results 8deCODE genetics Announces Third Quarter 2008 Financial Results 9deCODE genetics Announces Third Quarter 2008 Financial Results 10deCODE genetics Announces Third Quarter 2008 Financial Results 11deCODE genetics Announces Third Quarter 2008 Financial Results 12NeurogesX Reports Third Quarter 2008 Results 2NeurogesX Reports Third Quarter 2008 Results 3NeurogesX Reports Third Quarter 2008 Results 4NeurogesX Reports Third Quarter 2008 Results 5NeurogesX Reports Third Quarter 2008 Results 6NeurogesX Reports Third Quarter 2008 Results 7NeurogesX Reports Third Quarter 2008 Results 8NeurogesX Reports Third Quarter 2008 Results 9New Study Shares Best Practices in Biopharma Public Relations 2
(Date:7/31/2014)... adipocytes, or fat cells, are inherently different. Each of ... its own role in metabolism. In the human body, ... Its primary function is energy storage. On the other ... but are only found in a few places in ... special type of brown adipocytes, appear mixed with brown ...
(Date:7/31/2014)... studies have shown that marine noise can affect animal ... the Universities of Bristol and Exeter and the cole ... demonstrated that boat noise stops embryonic development and increases ... the sea slug Stylocheilus striatus used in ... away and later feed on toxic alga but this ...
(Date:7/30/2014)... epigenetic processes to regulate its genes, and adapt to ... of the genome regulation taking place in the truffle, ... Biology and illustrates how the truffle deals with ... authors say this may shed light on how traits ... (Tuber melanosporum), also known as Prigord truffles, have a ...
Breaking Biology News(10 mins):Hope for the overweight 2Boat noise impacts development and survival of sea hares 2How black truffles deal with the jumpers in their genome 2
... Fast development is often perceived as an advantage, ... readiness to cope with the challenges that it poses. ... and University of California, Santa Cruz, and published in ... the acceleration of developmental rate incurs potentially lethal physiological ...
... molding some snow into a ball and hurling it or ... can? New research from Indiana University and the University of ... readily learn to throw long distances. This research also suggests ... in a way critical to our very existence. ...
... N.C. Despite what you might have heard, genetic sequencing ... Duke University Medical Center have shown that functional tests are ... of sequencing studies as they relate to disease, using a ... patients to have many different traits. "Right now ...
Cached Biology News:Faster development might have its costs 2Faster development might have its costs 3IU study: Humans' critical ability to throw long distances aided by an illusion 2IU study: Humans' critical ability to throw long distances aided by an illusion 3Genetic sequencing alone doesn't offer a true picture of human disease 2Genetic sequencing alone doesn't offer a true picture of human disease 3
Angiotensin Converting Enzyme [Porcine Kidney, ACE, Peptidyl-dipeptidase A]...
Oven mesh sheets, large, 23 x 23cm, pack of 5...
Preparation Note: Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948). Quality Clarifier: Contains lipase Unit Definition: One unit will hydrolyze 1.0 μmole of p-ni...
... Analysis Note: Protein determined by Biuret ... 25 mM Tris-HCl, pH 7.6, 1 mM ... 2 Preparation Unit Definition: One unit ... per minute at pH 9.8 at 37 ...
Biology Products: