Navigation Links
Protein-printing technique gives snapshots of immune system defense
Date:11/3/2008

p>To test the idea, Love, visiting scientist Craig Story and their colleagues put living mouse B cells into a microengraved rubber device like the tiniest and densest of ice cube trays. There, 20,000 cells, separated into individual compartments, churned out their unique brands of antibodies produced after a series of immunizations.

The researchers sealed the tiny chambers with microarraysglass microscope slides coated with capture antibodies. The secreted proteins stuck to the glass, seeding it like a printer's plate being imbued with ink.

Next, the scientists stamped out multiple copies of these microarrays. Studying these, the scientists can examine the antibodies expressed by a single cell, in the context of a whole population of cells. The information is extracted and integrated by customized software and the large sets of data are examined much as they are with DNA microarrays.

The first results, imaged and analyzed by graduate student Eliseo Papa of the Harvard/MIT Health Science and Technology Institute, appeared in PNAS online the week of November 3. They gave a snapshot of the diversity of B cells that were generated by a series of immunizations designed to mimic a multi-part vaccination.

This technique could prove a powerful tool for research and medical testing. Currently, "the only accepted measure of immunological protection is a relatively crude test for whether antibodies are present in the blood," Ploegh says. "Knowing not only the level of antibody in a patient's blood but also how effective the antibodies would be in fighting a specific disease would be a big step."

Knowledge gained this way would be vastly helpful for vaccine development. "It's extremely important to have a rapid test to interrogate a vaccine recipient's immune system," allowing widespread testing among a given human population, says Ploegh.

"Nobody really knows in detail what happens between vaccine boosters," ad
'/>"/>

Contact: Eric Bender
bender@wi.mit.edu
617-258-9183
Whitehead Institute for Biomedical Research
Source:Eurekalert

Page: 1 2 3

Related biology technology :

1. Prominent Urologist Dr. David B. Samadi Presents Breakthrough Robotic Surgery Techniques on OR Live Webcast
2. Nanoscale dimensioning is fast, cheap with new NIST optical technique
3. Like an arrow: Jumping insects use archery techniques
4. Parallel nano-soldering technique chosen for years top-50 by Nanotech Briefs
5. Pioneering IVF Technique Produces Region's First Pregnancy
6. New technique to compress light could open doors for optical communications
7. New technique produces genetically identical stem cells
8. On the boil: New nano technique significantly boosts boiling efficiency
9. UBC physicists develop impossible technique to study and develop superconductors
10. New molecular imaging techniques may lead to advances in disease treatment
11. New technique measures ultrashort laser pulses at focus
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/4/2015)... 4, 2015  Brooklyn residents now have a new ... pain and injuries. Nunzio Saulle , M.D., a ... of his NJS Physical Medicine and Rehabilitation (PM&R) practice ... March 5, 2015. Health Plus Management, LLC is managing ... at 1178 Flatbush Avenue. This new PM&R practice will ...
(Date:3/4/2015)... /CNW/ - Resverlogix Corp. (TSX:RVX) (the "Company") today ... research program with Emerald Logic, a leader ... Technology (FACET), Emerald Logic analyzed Resverlogix,s complete ... each of 798 patients who participated in ... SUSTAIN and ASSURE. The objective of this ...
(Date:3/4/2015)... Pa. , March 4, 2015 /PRNewswire/ ... services and technology provider, today announced that ... 10 countries convened at its annual European ... to discuss the use of ... challenges. BioClinica,s eClinical technologies include: the Microsoft ...
(Date:3/4/2015)... Lancée par l,Assistance ... en décembre 2011, l,étude collaborative française randomisée ... compare   l es microsphères en résine ... le carcinome hépatocellulaire avancé, a recruté plus de ...   ; les résultats sont attendus fin 2016. ...
Breaking Biology Technology:NJS Physical Medicine & Rehabilitation Opening on Flatbush Avenue, Brooklyn, NY 2Resverlogix Collaborates with Emerald Logic to Identify Factors Driving Drug Response and Efficacy in Phase 2 Program 2Resverlogix Collaborates with Emerald Logic to Identify Factors Driving Drug Response and Efficacy in Phase 2 Program 3Resverlogix Collaborates with Emerald Logic to Identify Factors Driving Drug Response and Efficacy in Phase 2 Program 4BioClinica Teams With Pharma To Tackle Clinical Trial Challenges In Europe 2BioClinica Teams With Pharma To Tackle Clinical Trial Challenges In Europe 3Traitement du cancer primitif du foie : Recrutement terminé pour l'étude SARAH et résultats attendus fin 2016 2Traitement du cancer primitif du foie : Recrutement terminé pour l'étude SARAH et résultats attendus fin 2016 3Traitement du cancer primitif du foie : Recrutement terminé pour l'étude SARAH et résultats attendus fin 2016 4Traitement du cancer primitif du foie : Recrutement terminé pour l'étude SARAH et résultats attendus fin 2016 5Traitement du cancer primitif du foie : Recrutement terminé pour l'étude SARAH et résultats attendus fin 2016 6
... ,II. Product Components ,III. Materials to be ... of DNA from Whole Blood ,V. Protocol for ... Quantification of Amplified Products ,VII. Purification of Amplified ... , Contact Information , , , , ...
... Using siRNA for gene silencing is a, ... for preparing siRNA, such as chemical synthesis, in vitro transcription, ... cassettes. Irrespective, of which method ... to choose the siRNA target site. The guidelines below for choosing, ...
... , , ... , , This Application Note describes ... total RNA and fragmented cRNA by using,the Agilent 2100 bioanalyzer. The Eukaryote ... 28S/18S ratio in the,total RNA as well as detection of possible degradation. ...
Cached Biology Technology:Protocol for GenomePlex Whole Genome Amplification ,from Whole Blood 2Protocol for GenomePlex Whole Genome Amplification ,from Whole Blood 3Protocol for GenomePlex Whole Genome Amplification ,from Whole Blood 4Protocol for GenomePlex Whole Genome Amplification ,from Whole Blood 5Protocol for GenomePlex Whole Genome Amplification ,from Whole Blood 6siRNA Design Guidelines 2siRNA Design Guidelines 3siRNA Design Guidelines 4siRNA Design Guidelines 5siRNA Design Guidelines 6siRNA Design Guidelines 7Advancing the quality control methodology,to assess isolated total RNA,and generated fragmented cRNA 2Advancing the quality control methodology,to assess isolated total RNA,and generated fragmented cRNA 3Advancing the quality control methodology,to assess isolated total RNA,and generated fragmented cRNA 4Advancing the quality control methodology,to assess isolated total RNA,and generated fragmented cRNA 5Advancing the quality control methodology,to assess isolated total RNA,and generated fragmented cRNA 6
(Date:1/22/2015)... Jan. 13, 2015  Today, FreeWavz ( www.FreeWavz.com ), ... crowdfunding campaign on Fundable, https://www.fundable.com/freewavz . FreeWavz ... production capacity to meet customer demand. ... http://photos.prnewswire.com/prnh/20150113/168790 Invented by ...
(Date:1/22/2015)... WINSTON-SALEM, N.C. , Jan. 16, 2015  A man-made ... working memory and other mental capabilities in adults with ... a pilot study led by researchers at Wake Forest ... 60 adults diagnosed with amnesic mild cognitive impairment (MCI) ...
(Date:1/22/2015)... , Jan. 21, 2015  Analyst Report Issued by Small ... Brands report showed that planet-wide, transactions at merchants on the ... the total number of credit, debit, and prepaid cards reached ... were victims of fraudulent card usage in 2012, and credit ...
Breaking Biology News(10 mins):FreeWavz Launches on Fundable to Drive Speed-to-Market in 2015 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 3$4.80 Near Term Price Target with "Buy" given by CapRock Research for NXT-ID, Inc. (NASDAQ: NXTD) issued by Small Cap IR 2
... and Workgroup Scanners Recognized by ... the Leading Guide for Office ... Fujitsu Computer Products of,America, Inc., a market leader in ... scanners earned the "Editor,s Choice",designation in the Better Buys ...
... Bacteria save energy by producing proteins that moonlight, having ... the microbes from being killed. The moonlighting activity of ... resistant to a family of broad-spectrum antibiotics, according to ... journal Microbiology. "Glutamate racemase, or ...
... 2008The many recent reports documenting the therapeutic efficacy of ... disease may actually be describing non-specific therapeutic effects related ... response, according to a paper in the September 2008 ... Therapy , a peer-reviewed journal published by Mary Ann ...
Cached Biology News:Fujitsu Scanners Selected as 'Editor's Choice' in Better Buys for Business 2008 Scan-to-File Guide 2Fujitsu Scanners Selected as 'Editor's Choice' in Better Buys for Business 2008 Scan-to-File Guide 3Energy-saving bacteria resist antibiotics 2Previous claims of siRNA therapeutic effects called into question by report in human gene therapy 2
... Ambions MAXIscript In Vitro Transcription Kits ... 1 x 10 9 cpm/g. High ... in ribonuclease protection assays, in situ hybridization, ... be used for the synthesis of modest ...
Mouse H60 Allophycocyanin MAb (Clone 205326) ENTREZ GeneID: 15101...
... 250 ul (125 precipitations). ... easy pellet location.Efficient precipitation ... inert, compatible with most ... protocol. Category: DNA/RNA Synthesis ...
... for monitoring Aldolase tests. 2 levels ... targeted manufacturing values at 4 ± ... IU/L. 30-day shelf life once ... Level 1: 3 x 3 ml; ...
Biology Products: