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Next Generation Sequencing: Emerging Clinical Applications and Global Markets
Date:7/25/2013

TABLE 5 CLINICAL NEXT GENERATION SEQUENCING APPLICATION STATUS 16

TABLE 6 KEY DIAGNOSTIC NEXT GENERATION SEQUENCING INDUSTRY

PARTICIPANTS BY SECTOR 17

TABLE 7 DNA CHEMICAL BUILDING BLOCKS 20

TABLE 8 DNA TRANSLATION AND TRANSCRIPTION 20

TABLE 9 LONG-RANGE DNA STRUCTURE 21

TABLE 10 GENETIC VARIANT TYPES 22

TABLE 11 IMPORTANT MOLECULAR DIAGNOSTIC PLATFORMS BY COMPANY 23

TABLE 12 GENETIC TESTING EVOLUTION 24

TABLE 13 KEY NEAR- TO MID-TERM SEGMENTS FOR NEXT GENERATION

SEQUENCING DIAGNOSTICS 25

TABLE 14 DNA SEQUENCING HISTORICAL TIMELINE, 1977-2013 27

TABLE 15 SEQUENCING TECHNOLOGY DEVELOPMENT, 1990–2013 32

TABLE 16 EFFECT OF NEW SEQUENCING TECHNOLOGIES ON COST AND TIME TO

SEQUENCE A SINGLE HUMAN GENOME, 2001 AND 2013 ($) 33

TABLE 17 SANGER SEQUENCING SUMMARY 33

TABLE 18 SANGER SEQUENCING IMPROVEMENTS 34

TABLE 19 NEXT GENERATION SEQUENCING TECHNOLOGIES 35

TABLE 20 ROCHE NEXT GENERATION SEQUENCING WORKFLOW 35

TABLE 21 ILLUMINA NEXT GENERATION SEQUENCING WORKFLOW 36

TABLE 22 LIFE TECHNOLOGIES NEXT GENERATION SEQUENCING WORKFLOW 37

TABLE 23 BENCHTOP SEQUENCER PLATFORMS , 2010-2012 38

TABLE 24 NEXT GENERATION SEQUENCING BIOINFORMATICS WORKFLOW 39

TABLE 25 SEQUENCE ANNOTATION STEPS 41

TABLE 26 FUTURE CHALLENGES FOR IMPLEMENTING CLINICAL NEXT GENERATION

SEQUENCING 42

TABLE 27 TOP 10 CLINICAL NEXT GENERATION SEQUENCING PROJECTS 43

TABLE 28 CLINICAL NEXT GENERATION SEQUENCING NEAR- TO MID-TERM

APPLICATIONS 50

TABLE 29 CANCER APPLICATIONS 51

TABLE 30 MENDELIAN DISORDERS APPLICATIONS 53

TABLE 31 SCREENING APPLICATIONS 54

TABLE 32 ETHICAL ISSUES ASSOCIATED WITH PRENATAL AND NEWBORN

SCREENING 57

TABLE 33 INFECTIOUS DISEASE APPLICATIONS 57

TABLE 34 COMMON DISORDER APPLICATIONS 60

TABLE 35 KEY SEGMENTS WITHIN THE NEXT GENERA
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Applications include ELISA, immunochemistry, and Western blotting. Working Concentrations: ELISA: 0.005-0.05 U/ml, immunohisto/cytochemistry: 0.25-0.5 U/ml, Western blot: 0.1-0.25 U/ml....
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