Navigation Links
New study validates longevity pathway
Date:3/7/2013

hemical group, one of which is tryptophan, a molecule abundant in turkey and notable for inducing drowsiness. When researchers repeated the experiment, swapping the fluorescing chemical group on the substrate with a tryptophan residue, resveratrol and similar molecules were once again able to activate SIRT1.

"We discovered a signature for activation that is in fact found in the cell and doesn't require these other synthetic groups," said Hubbard, first author of the study. "This was a critical result, which allowed us to bridge the gap between our biochemical and physiological findings.

"Next, we needed to identify precisely how resveratrol presses on SIRT1's accelerator," said Sinclair. The team tested approximately 2,000 mutants of the SIRT1 gene, eventually identifying one mutant that completely blocked resveratrol's effect. The particular mutation resulted in the substitution of a single amino acid residue, out of the 747 that make up SIRT1. The researchers also tested hundreds of other molecules from the Sirtris library, many of which are far more powerful than resveratrol, against this mutant SIRT1. All failed to activate it.

The authors propose a model for how resveratrol works: When the molecule binds, a hinge flips, and SIRT1 becomes hyperactive.

Although these experiments occurred in a test tube, once the researchers identified the precise location of the accelerator pedal on SIRT1and how to break itthey could test their ideas in a cell. They replaced the normal SIRT1 gene in muscle and skin cells with the accelerator-dead mutant. Now they could test precisely whether resveratrol and the drugs in development work by tweaking SIRT1 (in which case they would not work) or one of the thousands of other proteins in a cell (in which they would work). While resveratrol and the drugs tested revved up mitochondria in normal cells (an effect caused activating by SIRT1), the mutant cells were completely immune.

"This
'/>"/>

Contact: David Cameron
david_cameron@hms.harvard.edu
617-432-0441
Harvard Medical School
Source:Eurekalert

Page: 1 2 3 4

Related biology technology :

1. Advanced Cell Diagnostics Initiates Agreement to Study Biomarkers for Cancer Immunotherapy
2. iLab Solutions Conducts Its Third Annual Core Facility Benchmarking Study
3. University of Colorado Cancer Center Study Shows the Preferred Feeding Method is not the Medically Best in Malnourished Pediatric Cancer Patients
4. University of Colorado Cancer Center Study Shows That Problem-Solving Training Helps Mothers Cope with Child’s Cancer Diagnosis
5. ADVENTRX Initiates Thorough QT/QTc Clinical Study Of ANX-188
6. Shire Regenerative Medicine Initiates Phase 3 Study of ABH001 for Patients with Epidermolysis Bullosa
7. New Study Shows Impressive Results with Abutment-Free Sophono System
8. EU Study Finds UK Health Product Benefit Metric Flawed
9. Notre Dame study explores the potential benefits and threats of nanotechnology research
10. CSL Behring enrolls first patient in global pediatric Phase III pivotal study of recombinant fusion protein linking coagulation factor IX with albumin (rIX-FP) to treat hemophilia B
11. University of Colorado Cancer Center Study Finds That Body's Ibuprofen, SPARC, Reduces Inflammation and Thus Bladder Cancer Development and Metastasis
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/27/2014)... , Nov. 27, 2014 Research ... "Global Palmitic Acid Industry Report 2014" report ... The Global Palmitic Acid Industry Report ... current state of the global palmitic acid industry. ... industry including definitions, classifications, applications and industry chain structure. ...
(Date:11/27/2014)... 2014 Spartan Bioscience today announced that ... Drug Safety (MFDS) regulatory approval for its Spartan ... in less than 60 minutes from a non-invasive cheek ... aid for doctors to determine therapeutic strategies for drugs ... some of the world’s most commonly prescribed drugs such ...
(Date:11/27/2014)... England , November 27, 2014 ... online on ScienceDirect   Elsevier , ... products and solutions, is pleased to announce the launch ... . The launch of ... the rapid advances at the forefront of applied sciences ...
(Date:11/27/2014)... , November 27, 2014 ... revolutionising the management of tooth decay, today announces ... of its Calcivis® Caries Activity Imaging System. ... a sophisticated medical device and consumable combination designed ... caries (tooth decay). It involves a unique, proprietary ...
Breaking Biology Technology:Global Palmitic Acid Industry Report 2014 2Spartan Bioscience Receives Korean Regulatory Approval for First Rapid DNA Test of Mutations Affecting Metabolism of Common Drugs 2Spartan Bioscience Receives Korean Regulatory Approval for First Rapid DNA Test of Mutations Affecting Metabolism of Common Drugs 3Elsevier Announces the Launch of a New Journal: Extreme Mechanics Letters 2Elsevier Announces the Launch of a New Journal: Extreme Mechanics Letters 3Calcivis Completes Clinical Study of Novel Caries Activity Imaging System 2Calcivis Completes Clinical Study of Novel Caries Activity Imaging System 3
... RNA later Tissue Collection: RNA , ... , Problem: ... processing or appropriate storage of the tissue or cell , ... followed by immediate disruption in lysis buffer. , ...
... The first step in preparing a plasmid for siRNA , ... of interest , that are susceptible to ... a little more than half of the siRNAs provide at least a 50% , ... siRNAs , provide a 75-95% reduction. The ...
... dsRNA that , most efficiently silences the gene ... correlate with silencing efficiency? Ambion scientists , addressed ... dsRNA of different sizes and directed , ... (Figure 1A), were prepared using the MEGAscript RNAi Kit , ...
Cached Biology Technology:Stabilizing RNA in Fresh and Frozen Tissues for RNA Isolation 2Stabilizing RNA in Fresh and Frozen Tissues for RNA Isolation 3Stabilizing RNA in Fresh and Frozen Tissues for RNA Isolation 4Selecting siRNA Sequences to Incorporate into the pSilencer Vectors 2Selecting siRNA Sequences to Incorporate into the pSilencer Vectors 3Selecting siRNA Sequences to Incorporate into the pSilencer Vectors 4RNAi: Size Does Matter 2
(Date:11/21/2014)... According to a new market ... RFID, Access Control, Intrusion Detection, Parking Management, Under Vehicle ... Service Geography - Global Forecasts to 2020", published by ... be around $25 Billion in 2014 and is expected ... CAGR of 8.69%. Browse 116 market data ...
(Date:11/21/2014)... Nov. 19, 2014  Earlier this year Donald ... College, and one of the most prolific inventors in ... are transmitted from Smartphones to third party agencies. Spector ... has one of the earliest known patents in this ... in the military, child care, elder care and hospital ...
(Date:11/21/2014)... Strict laws against distracted driving and the ... American and European automotive sector towards gesture recognition technologies. ... that are intuitive and able to retrieve information with ... New analysis from Frost & Sullivan,s Strategic ... Europe and North America ...
Breaking Biology News(10 mins):Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 2Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 3Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 4
... Associate Professor Michael King of the University of Rochester ... the blood for cancer and stem cells. When ... he captures stem cells, he harvests them for later ... applications that treat human disease and improve health. ...
... By imaging patterns of activity in the brain's prefrontal ... future actions, researchers have been able to distinguish cortical ... The findings, reported by a collaborative group led by ... Cognitive and Brain Sciences in Leipzig, Germany, appear in ...
... illusions are produced in the brain's visual cortex, researchers at ... rapid integration of auditory and visual sensations in the brain. ... by which visual perception can be altered by concurrent auditory ... of the Journal of Neuroscience. , When subjects were ...
Cached Biology News:Cancer-killing invention also harvests stem cells 2Cancer-killing invention also harvests stem cells 3Cancer-killing invention also harvests stem cells 4Reading hidden intentions in the human brain 2Wired for sound: How the brain senses visual illusions 2