Farmingdale, NY (PRWEB) June 25, 2013
The Spectral Bandwidth (SBW) of a spectrophotometer is the basis of establishing its ability to resolve absorption lines separated by small differences in wavelength. If a substance that requires measurement has an absorption line at 257.4nm and there is an interfering line at 260.0 nm, one needs to establish whether the spectrophotometer can resolve the two lines separately or if it "mixes" the two absorption lines into a single line. This brings about erroneous results. Toluene in Hexane is used as a reference for the calculation of SBW. The ratio of the absorption of the solution when read at a maxima (around 268.7nm) and at a minima (around 267.0nm) relates directly to the SBW of the instrument being assessed. Regular use of this technique will assure that the resolution of your instrument is within the required range for Good Laboratory Practice.
Assessing the photometric and wavelength performance of a UV/VIS spectrophotometer is a necessary part of keeping a laboratory in accordance with GLP standards which makes NSG Precision Cells' Toluene in Hexane a necessity. Understanding and accounting for how expanded uncertainties affect these measurements is a critical component of the calibration process.
NSG's Toluene in Hexane reference standard ensures accurate wavelength and absorbance results by verifying the spectrophotometer's resolution. Accurate absorbance and wavelength measurements can only be achieved if due consideration is given to the resolution of the monochromator in use. In modern instruments with grating monochromato
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