Navigation Links
Improving microscopy by following the astronomers' guide star
Date:2/17/2011

fluorescent bead guide stars. After the mice mature, Ji builds a window into the brain by removing a piece of skull and replacing it with a clear glass cover. She then uses the adaptive optical microscope she and Betzig built to peer through the glass and image the individual neurons underneath.

One of the concerns the researchers faced when imaging a live animal was how long a correction would persist. In astronomy, stars twinkle so fast that up to a thousand corrections are needed per second. Fortunately, Ji and Betzig learned that, for an anesthetized mouse, a single correction would remain valid for nearly an hour. Another question was whether the correction made at a single guide star bead could be applied to surrounding areas. Although the answer varies from sample to sample, Ji's work has shown that a single correction will often apply to a space of over 100 micrometers in each direction, a volume that can fit dozens of neurons. "It would be prohibitively slow if you had to correct at every point throughout an entire volume," Betzig says. To address this same problem in the field of astronomy, scientists employ a series of deformable mirrors that each look at guide stars in different directions. Betzig hopes to use a similar strategy to widen the correction even further in his microscope.

Since arriving at Janelia Farm in 2005, Betzig and his colleagues have pioneered new super high-resolution imaging techniques and shared them with biologists. One of them, photoactivated localization microscopy or PALM, maps individual protein molecules to produce images with 10-20 times the resolution of a traditional light microscope. PALM and other types of imaging, such as confocal microscopy and wide-field imaging, can be greatly improved with adaptive optics, he says. This is only the beginning. "What we do is primitive compared to the sophistication of what they do in the astronomy community," Betzig says. "I still feel we have a lot to learn f
'/>"/>

Contact: Andrea Widener
widenera@hhmi.org
301-215-8807
Howard Hughes Medical Institute
Source:Eurekalert

Page: 1 2 3 4

Related biology technology :

1. Iowa State, Ames Lab physicist developing, improving designer optical materials
2. 52-Week Phase 3 Study Found Investigational Drug Dapagliflozin Plus Metformin Similar to Glipizide Plus Metformin in Improving Glycosylated Hemoglobin (HbA1c) in Adults with Type 2 Diabetes Mellitus
3. Caliper Owners Group Meeting Showcases Critical Role of Biopharma and Academic Researchers for Improving Healthcare and Quality of Life
4. Plant BioTech World Congress to Highlight Scientific Discoveries and Technologies Bringing New Hope to Feeding the World and Improving Lives
5. Elsevier Addresses Todays Healthcare Information Crisis With a Renewed Focus on Improving Quality and Efficiency
6. Soybean genome analysis reveals pathways for improving biodiesel
7. Nanomedicine Company Focuses on Improving Premature Infant Health and Reducing Death Rate
8. Improving Transfusion Safety: Fenwal, Verax Biomedical Sign Agreement for Rapid, Point-of-Care Bacteria Test
9. 2009: Immunotec announces important clinical research initiative: New study to be launched with the goal of improving the quality of the aging process
10. ClearTrial Industry Expert to Lead Session at DIA Annual Meeting on Improving Accrual and Contract Management in Clinical Trials
11. AARC Presentation Cites Masimo Patient SafetyNet as Key Factor in Improving Patient Outcomes and Reducing Costs in Post-Acute Care Facilities
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/2/2014)... 2, 2014  Xencor, Inc. (NASDAQ: ... monoclonal antibodies for the treatment of autoimmune diseases, ... the appointment of Debra Zack , M.D., ... of Lloyd Rowland , senior vice president, ... "Debra,s expertise in biologics clinical development and ...
(Date:9/2/2014)... 2014  Synthetic Biologics, Inc. (NYSE MKT: SYN), ... candidates targeting specific pathogens that cause serious infections ... final preclinical toxicology study of SYN-004. Per U.S. ... study was required to move the Company,s lead ... devastating effects of Clostridium difficile (C. difficile), ...
(Date:9/2/2014)... (PRWEB) September 02, 2014 PMG ... its 11th clinical research site: PMG Research of Rocky ... and operation of the clinical research department at ... PMG’s presence in eastern North Carolina. This new site ... the patients in Rocky Mount and the surrounding communities, ...
(Date:9/2/2014)... , September 2, 2014 Culot ... research and lab management services  BioData, producer ... is pleased to announce the appointment of Louis ... recently served as VP Marketing and Business Development at ... research and clinical applications. Previously, Culot worked ...
Breaking Biology Technology:Xencor Appoints Debra Zack, M.D., Ph.D., Vice President, Clinical Development and Lloyd Rowland, Senior Vice President, Chief Compliance Officer and General Counsel 2Xencor Appoints Debra Zack, M.D., Ph.D., Vice President, Clinical Development and Lloyd Rowland, Senior Vice President, Chief Compliance Officer and General Counsel 3Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 2Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 3Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 4Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 5PMG Research Announces the Formation of its Eleventh Site 2PMG Research Announces the Formation of its Eleventh Site 3Louis Culot Appointed as BioData's CEO 2
... 4, 2011 Genesis Biopharma, Inc. (OTC Bulletin Board: ... cancer therapies, today announced that it closed private offerings ... entered into definitive agreements with accredited investors to purchase ... common stock for a purchase price of $1.00 per ...
... Regeneron Pharmaceuticals, Inc. (Nasdaq: REGN ) ... Morgan Healthcare Conference on Monday, January 10, 2011.  The ... p.m. Eastern Time).  The session may be accessed through ... Investor Relations page.  An archived version of the presentation ...
... ST. LOUIS, Dec. 30, 2010 Sigma-Aldrich® Corporation ... has acquired all outstanding shares of Cerilliant Corporation ... analytical chemistry product portfolio and to continue to ... leading supplier of high quality reference standards and ...
Cached Biology Technology:Genesis Biopharma Raises $845,000 in Private Financings 2Sigma-Aldrich Acquires Cerilliant Corporation to Strengthen its Analytical Chemistry Portfolio 2Sigma-Aldrich Acquires Cerilliant Corporation to Strengthen its Analytical Chemistry Portfolio 3Sigma-Aldrich Acquires Cerilliant Corporation to Strengthen its Analytical Chemistry Portfolio 4
(Date:9/2/2014)... between our immune systems and cholera bacteria, humans may ... phages. In a new study, researchers from Tufts University, ... Health Laboratory, and elsewhere, report that phages can force ... to survive. Importantly, the study published in ... phage predation occurs during human infection. , First author ...
(Date:9/2/2014)... WASHINGTON, September 2, 2014 Best-selling author Sam Kean ... of the Megalodon, the 50-foot super shark that, despite ... long extinct. Learn all about it at http://youtu.be/KhFygIoW_MA ... True Tales of Madness, Love and the History of ... is getting the Reactions treatment in a 10-episode video ...
(Date:9/2/2014)... touch a hot oven, you rapidly pull your hand ... in sensing and responding to such painful stimuli, they ... researchers have made a surprising discovery about the role ... and have built a structural model of the molecule. ... , may help direct new strategies to treat pain ...
Breaking Biology News(10 mins):War between bacteria and phages benefits humans 2War between bacteria and phages benefits humans 3Surprising new role for calcium in sensing pain 2Surprising new role for calcium in sensing pain 3
... identifying a novel compound that primes a plant,s immune system, ... Chicago may be on a path to developing disease-resistant plants. ... team that includes Tim Tschaplinski of the Department of Energy,s ... the immunity response in Arabidopsis, a small flowering plant related ...
... tumors that arise from glial (supporting) cells of the ... grow fine extensions that infiltrate normal brain tissue and, ... surrounding tissue. Therefore, it is almost impossible to remove ... radical surgical removal of the tumor would substantially improve ...
... in German . Europe,s borders have ... from other parts of the world: from the American ... on, hybridise with, parasitise and out-compete native species. They ... landscapes and impact upon agriculture, forestry and fisheries. ...
Cached Biology News:Researchers discover primer to plant defense system 2Fluorescent cancer cells to guide brain surgeons 2Will Europe at last unite to combat thousands of alien invaders? 2Will Europe at last unite to combat thousands of alien invaders? 3
... These versatile vacuum cups provide flexible ... curved, or irregular surfaces. ,PFG: Flat ... Bellows cup ,PCG: Multiple Bellows cup ... from 25 to 200 mm , ...
Curcumin is an extract of turmeric root with antioxidant, anti-tumor promoter, and anti-inflammatory properties. Curcumin inhibits the induction of NOS in macrophages....
UGT1A6 (D-20)...
... smallest electronic pressure controller available measuring 26 ... this unit specifically for the analytical instrumentation ... carrier gas flow control, microfluidic flow control, ... focusing, the OEM-EPC offers external pressure sensor ...
Biology Products: