Navigation Links
Improving microscopy by following the astronomers' guide star
Date:2/17/2011

fluorescent bead guide stars. After the mice mature, Ji builds a window into the brain by removing a piece of skull and replacing it with a clear glass cover. She then uses the adaptive optical microscope she and Betzig built to peer through the glass and image the individual neurons underneath.

One of the concerns the researchers faced when imaging a live animal was how long a correction would persist. In astronomy, stars twinkle so fast that up to a thousand corrections are needed per second. Fortunately, Ji and Betzig learned that, for an anesthetized mouse, a single correction would remain valid for nearly an hour. Another question was whether the correction made at a single guide star bead could be applied to surrounding areas. Although the answer varies from sample to sample, Ji's work has shown that a single correction will often apply to a space of over 100 micrometers in each direction, a volume that can fit dozens of neurons. "It would be prohibitively slow if you had to correct at every point throughout an entire volume," Betzig says. To address this same problem in the field of astronomy, scientists employ a series of deformable mirrors that each look at guide stars in different directions. Betzig hopes to use a similar strategy to widen the correction even further in his microscope.

Since arriving at Janelia Farm in 2005, Betzig and his colleagues have pioneered new super high-resolution imaging techniques and shared them with biologists. One of them, photoactivated localization microscopy or PALM, maps individual protein molecules to produce images with 10-20 times the resolution of a traditional light microscope. PALM and other types of imaging, such as confocal microscopy and wide-field imaging, can be greatly improved with adaptive optics, he says. This is only the beginning. "What we do is primitive compared to the sophistication of what they do in the astronomy community," Betzig says. "I still feel we have a lot to learn f
'/>"/>

Contact: Andrea Widener
widenera@hhmi.org
301-215-8807
Howard Hughes Medical Institute
Source:Eurekalert

Page: 1 2 3 4

Related biology technology :

1. Iowa State, Ames Lab physicist developing, improving designer optical materials
2. 52-Week Phase 3 Study Found Investigational Drug Dapagliflozin Plus Metformin Similar to Glipizide Plus Metformin in Improving Glycosylated Hemoglobin (HbA1c) in Adults with Type 2 Diabetes Mellitus
3. Caliper Owners Group Meeting Showcases Critical Role of Biopharma and Academic Researchers for Improving Healthcare and Quality of Life
4. Plant BioTech World Congress to Highlight Scientific Discoveries and Technologies Bringing New Hope to Feeding the World and Improving Lives
5. Elsevier Addresses Todays Healthcare Information Crisis With a Renewed Focus on Improving Quality and Efficiency
6. Soybean genome analysis reveals pathways for improving biodiesel
7. Nanomedicine Company Focuses on Improving Premature Infant Health and Reducing Death Rate
8. Improving Transfusion Safety: Fenwal, Verax Biomedical Sign Agreement for Rapid, Point-of-Care Bacteria Test
9. 2009: Immunotec announces important clinical research initiative: New study to be launched with the goal of improving the quality of the aging process
10. ClearTrial Industry Expert to Lead Session at DIA Annual Meeting on Improving Accrual and Contract Management in Clinical Trials
11. AARC Presentation Cites Masimo Patient SafetyNet as Key Factor in Improving Patient Outcomes and Reducing Costs in Post-Acute Care Facilities
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/25/2014)... Pa. , July 25, 2014 /PRNewswire-iReach/ -- ... and consulting for global corporations operating in highly ... host its first annual Global User Group Conference, ... historic Philadelphia, Pennsylvania at ... - http://photos.prnewswire.com/prnh/20140724/130263 This ...
(Date:7/24/2014)... New York, NY - July 24, 2014 -- Keryx ... results from the long-term, randomized, active control Phase 3 ... ferric iron-based phosphate binder, for the treatment of hyperphosphatemia ... The PERFECTED study (PhosphatE binding and iRon delivery with ... the Journal of the American Society of Nephrology ...
(Date:7/24/2014)... Calif. , July 24, 2014  Now available ... to home. Rehealth Regenerative Therapies , located in ... and other physically active people a new health option: ... Regardless of age, countless patients suffer from joint ... activities, such as muscle tears, torn rotator cuff, tennis ...
(Date:7/24/2014)... Security forces worldwide rely on sophisticated equipment, trained ... other public areas against terrorist attacks. A revolutionary ... about to make their job much easier. , ... Patolsky of Tel Aviv University ,s School of ... developed by the Herzliya company Tracense, picks up ...
Breaking Biology Technology:Xybion Announces It Will Host International User Conference in Philadelphia 2Xybion Announces It Will Host International User Conference in Philadelphia 3Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 2Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 3Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 4Stem Cells In Sports Injuries, Joints And Muscular Pain 2Stem Cells In Sports Injuries, Joints And Muscular Pain 3Nano-sized chip 'sniffs out' explosives far better than trained dogs 2
... , , , ... became the only hospital in the Midwest to acquire a dual console ... States to purchase the new system. , , ... the latest robotic-assisted surgery system that gives surgeons an enhanced three-dimensional view ...
... , , , , ... Inc. (Nasdaq: RGDX ) announced today that it has entered ... Partnership to raise approximately $4 million from the private placement of 3,057,907 ... $1.30 per share. The closing of the sale of the shares occurred ...
... , , , ... (NYSE: ARE ), Landlord of Choice to the Life ... long-term lease with Eli Lilly and Company as the anchor tenant ... Science Park ("the Alexandria Center"). Lilly has leased approximately 91,000 ...
Cached Biology Technology:St. John's Hospital Acquires Latest Technology in Robotic-Assisted Surgery 2Response Genetics Announces $4 Million Private Placement 2Response Genetics Announces $4 Million Private Placement 3Alexandria Real Estate Equities, Inc. Signs Biopharmaceutical Company, Lilly, as Anchor New York City Tenant at the Alexandria Center for Science and Technology 2Alexandria Real Estate Equities, Inc. Signs Biopharmaceutical Company, Lilly, as Anchor New York City Tenant at the Alexandria Center for Science and Technology 3Alexandria Real Estate Equities, Inc. Signs Biopharmaceutical Company, Lilly, as Anchor New York City Tenant at the Alexandria Center for Science and Technology 4
(Date:7/24/2014)... 24, 2014) Scientists at The New York ... step closer to creating a viable cell replacement ... cells. , For the first time, NYSCF ... from skin samples of patients with primary progressive ... protocol to induce these stem cells into becoming ...
(Date:7/24/2014)... Bushmeat, the use of native animal species for food ... be a significant factor in the decline of many ... new study indicates that more than half of the ... raptors and hornbills., "By surveying not only the meat ... being eaten inside the forest by hunters and brought ...
(Date:7/24/2014)... current biodiversity, the product of 3.5 billion years of ... history of life. But it may be reaching a ... literature and analysis of data published in Science ... loss and decline of animals is contributing to what ... sixth mass biological extinction event. , Since 1500, more ...
Breaking Biology News(10 mins):NYSCF scientists one step closer to cell therapy for multiple sclerosis patients 2Stanford biologist warns of early stages of Earth's 6th mass extinction event 2Stanford biologist warns of early stages of Earth's 6th mass extinction event 3
... pay more for meals prepared with produce and meat ... meals actually increases when the price increases, according to ... of how customers perceive and value local food shows ... when they are priced slightly higher than meals made ...
... a poorly understood class of RNA produced in a mammal,s ... of the infection. Their findings are reported today in ... Microbiology. RNA (ribonucleic acid) contains information transcribed from the ... these RNAs translate sections of DNA code into building blocks ...
... New University of Florida research puts to rest the ... glacial period. It turns out it ended up in the ... findings have implications for modern-day global warming, said Ellen Martin, ... paper, which is published in this week,s journal Nature ...
Cached Biology News:Restaurant customers willing to pay more for local food 2New class of biomolecules triggered in response to respiratory virus infection 2New class of biomolecules triggered in response to respiratory virus infection 3New class of biomolecules triggered in response to respiratory virus infection 4UF research gives clues about carbon dioxide patterns at end of Ice Age 2UF research gives clues about carbon dioxide patterns at end of Ice Age 3
Screening assay to detect the Listeria monocytogenes in food samples by,DNA amplification and molecular hybridization on a microtiter plate....
... CopyControl™ vector can be induced prior to ... provide the stability of single-copy BACs with ... other cloning vectors. The kits provide the ... insert size screening system) necessary to create ...
... PCR Enhancer (with betaine) substantially improves ... and specificity for amplification of many ... in PCR eliminates the base-pair composition ... thermal stability, suppresses pauses of DNA ...
... Systems provide rapid, precise localization of ... frozen or paraffin-embedded tissue, cytospins and ... facilitate double or triple labeleing experiments, ... with primary antibodies of different animal ...
Biology Products: