Navigation Links
Improving microscopy by following the astronomers' guide star
Date:2/17/2011

clearer view of the target. Today, adaptive optics instrumentation accompanies all of the world's major telescopes.

Unlike in astronomy, microscopists can't place a wavefront sensor within a live animal to directly measure the distortions of light deep within tissue. To get around this problem, Betzig reasoned that the perfect focus is nothing more than a bunch of rays converging from many different directions to the same identical point. The heterogeneity of tissue means that ray is deflected differently so they no longer meet at a single point. Betzig figured that if they could study the rays individually, they could correct their deflections and steer them back to a single focus.

For their 2009 study, Betzig and Ji buried fluorescent beads underneath thick slices of mouse brain. The beads act as guide stars to help measure the deflections of the rays. To do that, the pair use a one-inch display called a spatial light modulator. The display allows them to turn on one ray at a time and then take an image of the bead. They can then determine how much the ray is deflected from the amount the bead's image is shifted relative to the desired focal point. The display is then used like a small, tiltable mirror to steer the ray back to the focal point. The process is then repeated with each of the other rays. This strategy improves the fluorescent signal and recovers optimal resolution through a chunk of tissue up to 400 micrometers thick. "Another advantage is that it's very efficient in terms of how little light is needed," Betzig says. "Light is not completely noninvasive, so as microscopists we have to be very careful not to damage our specimens."

Ji, who will soon begin her own lab as a fellow at Janelia Farm, has recently taken the technique to the next level: live brain imaging in mice. To do this, she uses genetic engineering to label the brains of mouse fetuses with a fluorescent marker of neurons while at the same time injecting
'/>"/>

Contact: Andrea Widener
widenera@hhmi.org
301-215-8807
Howard Hughes Medical Institute
Source:Eurekalert

Page: 1 2 3 4

Related biology technology :

1. Iowa State, Ames Lab physicist developing, improving designer optical materials
2. 52-Week Phase 3 Study Found Investigational Drug Dapagliflozin Plus Metformin Similar to Glipizide Plus Metformin in Improving Glycosylated Hemoglobin (HbA1c) in Adults with Type 2 Diabetes Mellitus
3. Caliper Owners Group Meeting Showcases Critical Role of Biopharma and Academic Researchers for Improving Healthcare and Quality of Life
4. Plant BioTech World Congress to Highlight Scientific Discoveries and Technologies Bringing New Hope to Feeding the World and Improving Lives
5. Elsevier Addresses Todays Healthcare Information Crisis With a Renewed Focus on Improving Quality and Efficiency
6. Soybean genome analysis reveals pathways for improving biodiesel
7. Nanomedicine Company Focuses on Improving Premature Infant Health and Reducing Death Rate
8. Improving Transfusion Safety: Fenwal, Verax Biomedical Sign Agreement for Rapid, Point-of-Care Bacteria Test
9. 2009: Immunotec announces important clinical research initiative: New study to be launched with the goal of improving the quality of the aging process
10. ClearTrial Industry Expert to Lead Session at DIA Annual Meeting on Improving Accrual and Contract Management in Clinical Trials
11. AARC Presentation Cites Masimo Patient SafetyNet as Key Factor in Improving Patient Outcomes and Reducing Costs in Post-Acute Care Facilities
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/15/2014)... (PRWEB) January 15, 2014 Cynvenio, a ... management through the genomic analysis of tumor cells in ... Finder™, a web-based cancer decision support application powered by ... with Cynvenio’s ClearID™ genomic test, Therapy Finder will provide ...
(Date:1/15/2014)... Houston, TX (PRWEB) January 15, 2014 ... Alzheimer’s disease, and about 1 in 3 seniors will ... ( source ). These jaw-dropping figures have shocked many ... function and, hopefully, help prevent these tragic age-related cognitive ...
(Date:1/15/2014)... 15, 2014 Freeslate, Inc ., ... announced that Lupin Limited, one of India’s top five ... Protégé PharmD System for high throughput solid form ... focused on a wide range of quality, affordable generic ...
(Date:1/14/2014)... 2014 Histogen, Inc., a regenerative medicine ... grown under simulated embryonic conditions, today announced that they ... Medical, Inc. for physician-dispensed aesthetic products containing Histogen’s proprietary ... agreement is an amendment to the existing license between ...
Breaking Biology Technology:Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 2Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 3
... of the bacterium Caulobacter crescentus , researchers at the ... production factory, making useful proteins that can act as vaccines ... Society for General Microbiology,s meeting at Heriot-Watt University, Edinburgh, today ... bacterium that has a single protein layer on its surface. ...
... REDWOOD CITY, Calif., Sept. 4 Cardica, Inc. (Nasdaq: ... received a notice from the Listing Qualifications Department of The ... be delisted from NASDAQ based upon NASDAQ staff,s determination that ... stockholders, equity requirement for continued listing on the NASDAQ as ...
... BEIJING, Sept. 4 /PRNewswire-Asia/ -- Sinovac Biotech Ltd. ... products in China, announced today that,the Ministry of Industry ... issued the initial order to Sinovac to purchase H1N1 ... order, Sinovac is,required to complete the production of 3.3 ...
Cached Biology Technology:Cardica Receives NASDAQ Notification of Non-Compliance 2Sinovac Obtains Initial Order of H1N1 Vaccine from Chinese Central Government 2Sinovac Obtains Initial Order of H1N1 Vaccine from Chinese Central Government 3
(Date:4/17/2014)... A new modeling study suggests that fish consumption ... infant exposure to long-lived contaminants like persistent organic ... a team of researchers including University of Toronto ... Wania, looks at how different levels of environmental ... behavior of chemicals in the body influenced exposure ...
(Date:4/17/2014)... Kansas State University engineer has developed a patented ... improvised explosive devices. The same technique could help ... Steven M. and Kay L. Theede chair in ... engineering, and his research team have created a ... in car trunks. The distance detection method ...
(Date:4/17/2014)... Several parasites and pathogens that devastate honeybees in Europe, ... Africa, but do not appear to be impacting native ... team of researchers., The invasive pests include including ... East African honeybees appear to be resilient to these ... used to control pests in Europe, Asia and the ...
Breaking Biology News(10 mins):Fish consumption advisories fail to cover all types of contaminants 2Patented research remotely detects nitrogen-rich explosives 2East African honeybees are safe from invasive pests… for now 2East African honeybees are safe from invasive pests… for now 3
... PHILADELPHIAThe Academy of Natural Sciences today announced the revival ... an early 19th-century nature magazine of which only 13 complete ... Museum of Nature was published privately in London between 1810 ... one or two color plates. In it, Perry set out ...
... NY) Matthew Levy, Ph.D., assistant professor of biochemistry ... has been awarded more than $700,000 by Stand Up ... Levy,s work will focus on creating self-guiding drugs that ... serious or unpleasant side effects of current therapies. Dr. ...
... Just three months after the Euro 5 Norm ... car models, researchers at the Technische Universitaet Muenchen (TUM) ... meeting the more stringent Euro 6 emissions standard. A ... Chair of Internal Combustion Engines has succeeded in reducing ...
Cached Biology News:The Academy of Natural Sciences and Temple Press revive rare natural history work in new book 2Einstein receives high-risk/high-reward cancer research funding 2Einstein receives high-risk/high-reward cancer research funding 3Munich lab demonstrates diesel truck engine with barely measurable emissions 2Munich lab demonstrates diesel truck engine with barely measurable emissions 3
...
... polyclonal to MSY2/YBOX2 ( Abpromise ... Antigen: Synthetic peptide derived ... 178-227 of human YBX2. (Note: ... proprietary). ...
... EVO96R and Freedom EVO384R are fast multi-channel ... in three different sizes, with a multi-channel ... deliver significant time saving, with true parallel ... setup of your assays with the Freedom ...
... Aminopropylsilane is perhaps the ... chemistry for microarray experiments. ... for Microarrays provide a ... for printing PCR products ...
Biology Products: