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Diagenode Launches State-of-the-Art Solution for Shearing DNA Up to 100 kb Fragments for Long-Read Sequencing
Date:3/12/2019

Diagenode, a leading global provider for complete solutions for sample preparation and epigenetics research, has launched its third generation long-fragment DNA shearing innovation, the Megaruptor 3. This new solution builds upon the features of the second generation instrument with a user-friendly interface, automated processing, and the ability to process up to 8 samples simultaneously in a completely safe and closed system.

Prior to long-read sequencing on popular sequencing systems such as those from Oxford Nanopore or Pacific Biosciences, genomic DNA must be sheared to long fragment sizes. Though various methods are available for shearing genomic DNA, few are reliable for generating DNA fragments necessary for assembly of microbial, human, plant and animal genomes de novo.

The Megaruptor uses mechanical shearing to fragment DNA. DNA in solution is pumped through the device’s “hydropore” which has an array of uniform pores, stretching and breaking the DNA strands. DNA molecules are passed several times through the pores to guarantee a minimum and uniform length. Additionally, the hydropore design ensures that DNA will not clog the pores, resulting in consistent and efficient shearing not realized by single orifice devices.

“The new Megaruptor 3 is essential for any researcher who is focusing on long-read de novo sequencing or detecting structural variants. The new instrument is designed to provide automated and consistent fragmentation of DNA from 5kb to 100kb, unlike any other existing technology,” said Jerome Kroonen, Ph.D., Global Marketing Manager. “In addition, the system’s flexible throughput allows researchers to process as few as one sample or as many as eight in one run.”

To read more about the Megaruptor 3, please visit http://www.diagenode.com. To learn more about Diagenode, please visit http://www.diagenode.com.

About Diagenode:
Diagenode is a leading global provider of complete solutions for epigenetics research, proteomics assays, next-generation sequencing, and biological sample preparation. The company has developed a comprehensive approach to gain new insights into RNA, DNA, chromatin, and protein analysis with experimentally-validated, easy-to-use solutions and services to give researchers the success needed to achieve best-in-class results.
Diagenode's product portfolio includes innovative shearing solutions for a number of applications such as DNA shearing from 150bp to 100kb, chromatin shearing, RNA and protein extraction, and cell lysis. In addition, the company offers automation instruments, reagent kits, and high quality antibodies to streamline DNA methylation, ChIP, ChIPmentation, ChIP-seq, and ATAC-seq, and CATS RNA-seq workflows. The company’s latest innovation includes a wide menu of epigenetics assay services for genome-wide and targeted chromatin and DNA methylation assays.

For more information about Diagenode, please visit the company’s website at http://www.diagenode.com.

Read the full story at https://www.prweb.com/releases/diagenode_launches_state_of_the_art_solution_for_shearing_dna_up_to_100_kb_fragments_for_long_read_sequencing/prweb16161078.htm.


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