Navigation Links
pTriEx™-4 DNA from Novagen

ProductspTriEx™-4 DNA from Novagen
Company Novagen
Item pTriEx™-4 DNA
Price $228.00
Description The pTriEx™ vectors combine the proven features of the pET, BacVector® and mammalian systems and provides all of the benefits and versatility of each expression system in one vector backbone. The pTriEx vectors have been extensively tested for performance with various target genes in all three expression systems. In each case, expression was similar to that obtained with system-specific vectors. The pTriEx™-4 vector1 is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector a single recombinant plasmid can be used to test expression in E. coli, insect and vertebrate cells. Transient vertebrate expression is mediated by the CMV immediate early enhancer and promoter2. For expression in insect cells, pTriEx-4 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. Expression can be induced in hosts such as NovaBlue by infecting with λCE6, a phage that constitutively expresses T7 RNA polymerase from the λpL and λlpI promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows IPTG based induction. Native protein can be expressed by cloning into the NcoI site, or native protein can be generated by cloning into the PshAI or SmaI sites and cleavage of the fusion protein with Enterokinase or Thrombin, respectively. The pTriEx-4 vector is also available as an Ek/LIC Cloning Kit.
1 patent pending
2 The CMV promoter is covered by U.S. Patent nos. 5,168,062 and 5,385,839 issued to the University of Iowa Research Foundation and is licensed for research use only.
Info NovagenNovagen
441 Charmany Drive
Madison, WI 53719
Customer Service: 800-526-7319
Tech Support: 800-207-0144
Fax Number: 608-238-1388
Web Site: http://www.novagen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. S-Protein Agarose from Novagen
2. STag Thrombin Purification Kit from Novagen
3. S-Protein Agarose from Novagen
4. S-Protein Agarose from Novagen
5. pET GST Fusion System 42 plus Competent Cells from Novagen
6. pET GST Fusion System 42 from Novagen
7. pET GST Fusion System 41 plus Competent Cells from Novagen
8. pET GST Fusion System 41 from Novagen
9. GST•Tag™ Antibody Plate from Novagen
10. Mouse Anti-GST Tag Monoclonal Antibody, Unconjugated from Novagen
11. pETcoco-1 System from Novagen
...
Used in May-Grunwald Giemsa stain....
Certified U.S. origin. Bovine Serum:, *Certified U.S. origin. Available raw, nonsterile filtered, or sterile filtered. , , *Sterility tested , , *Low Hemoglobin levels , , *Mycoplasma testing upon...
The Magnetic Separation Rack is designed to be used for small-scale separations using magnetic particles. ,Capacity: 6 microcentrifuge tubes....
Biology Products:
(Date:2/14/2017)... N.C. , Feb. 14, 2017  Wake Forest ... M.D., as its new chief executive officer (CEO). Freischlag ... CEO John D. McConnell , M.D., who last ... position at the Medical Center, after leading it since ... the full scope of Wake Forest Baptist,s academic health ...
(Date:2/10/2017)... 10, 2017 Research and Markets ... "Personalized Medicine - Scientific and Commercial Aspects" to their ... ... is integrated with therapy for selection of treatment as well ... and prevention of disease in modern medicine. Biochip/microarray technologies and ...
(Date:2/8/2017)... Inc. (NASDAQ: AWRE ), a leading supplier of ... quarter and year ended December 31, 2016. ... compared to $6.9 million in the same quarter last year. ... million compared to $2.6 million in the fourth quarter of ... $0.5 million, or $0.02 per diluted share, which compares to ...
Breaking Biology News(10 mins):
(Date:2/23/2017)... SAN RAFAEL, Calif., Feb. 23, 2017 ... of U.S. dollars, except per share data, unaudited)Three Months ... ChangeTotal BioMarin Revenue $ ...     22832%$ 1,117$   89026%Aldurazyme Net Product Revenue ... 906538%34823946%Naglazyme Net Product Revenue  ...
(Date:2/23/2017)... 2017  MIODx announced today that it has ... technologies from the University of California, San Francisco ... monitor a patient for response to immune checkpoint ... second license extends the technology with a method ... have an immune-related adverse event (IRAE) from their ...
(Date:2/23/2017)...  Imanis Life Sciences announced today the launch ... viruses for virotherapy research. These viruses are licensed ... vaccinia virus-based technology platform for research use. ... partnership with Genelux to offer researchers, for the ... in research," said Dr. Kah Whye Peng ...
(Date:2/23/2017)... (PRWEB) , ... February 23, 2017 , ... ... during the 12th annual Inventors Recognition Reception at Purdue Research Park ... a faculty member in recognition of outstanding contributions to, and success with, commercializing ...
Breaking Biology Technology: