Navigation Links
pTriEx™-4 DNA from Novagen

ProductspTriEx™-4 DNA from Novagen
Company Novagen
Item pTriEx™-4 DNA
Price $228.00
Description The pTriEx™ vectors combine the proven features of the pET, BacVector® and mammalian systems and provides all of the benefits and versatility of each expression system in one vector backbone. The pTriEx vectors have been extensively tested for performance with various target genes in all three expression systems. In each case, expression was similar to that obtained with system-specific vectors. The pTriEx™-4 vector1 is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector a single recombinant plasmid can be used to test expression in E. coli, insect and vertebrate cells. Transient vertebrate expression is mediated by the CMV immediate early enhancer and promoter2. For expression in insect cells, pTriEx-4 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. Expression can be induced in hosts such as NovaBlue by infecting with λCE6, a phage that constitutively expresses T7 RNA polymerase from the λpL and λlpI promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows IPTG based induction. Native protein can be expressed by cloning into the NcoI site, or native protein can be generated by cloning into the PshAI or SmaI sites and cleavage of the fusion protein with Enterokinase or Thrombin, respectively. The pTriEx-4 vector is also available as an Ek/LIC Cloning Kit.
1 patent pending
2 The CMV promoter is covered by U.S. Patent nos. 5,168,062 and 5,385,839 issued to the University of Iowa Research Foundation and is licensed for research use only.
Info NovagenNovagen
441 Charmany Drive
Madison, WI 53719
Customer Service: 800-526-7319
Tech Support: 800-207-0144
Fax Number: 608-238-1388
Web Site:
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. S-Protein Agarose from Novagen
2. STag Thrombin Purification Kit from Novagen
3. S-Protein Agarose from Novagen
4. S-Protein Agarose from Novagen
5. pET GST Fusion System 42 plus Competent Cells from Novagen
6. pET GST Fusion System 42 from Novagen
7. pET GST Fusion System 41 plus Competent Cells from Novagen
8. pET GST Fusion System 41 from Novagen
9. GST•Tag™ Antibody Plate from Novagen
10. Mouse Anti-GST Tag Monoclonal Antibody, Unconjugated from Novagen
11. pETcoco-1 System from Novagen
(Date:10/29/2015)... Today, LifeBEAM , a leader ... a global leader in technical performance sports clothing ... advanced bio-sensing technology. The hat will allow fitness ... biometrics to improve overall training performance. As a ... bring together the most advanced technology, extensive understanding ...
(Date:10/27/2015)... 27, 2015 Munich, Germany ... Mapping technology (ASGM) automatically maps data from mobile eye ... , so that they can be quantitatively analyzed ... Munich, Germany , October 28-29, 2015. SMI,s ... from mobile eye tracking videos created with SMI,s ...
(Date:10/26/2015)... , October 26, 2015 ... --> adds Biometrics Market ... 2021 as well as Emerging Biometrics ... reports to its collection of IT ... . --> ...
Breaking Biology News(10 mins):
(Date:11/24/2015)... Israel , Nov. 24, 2015  Tikcro Technologies Ltd. (OTCQB: TIKRF) ... on December 29, 2015 at 11:00 a.m. Israel ... Electra Tower, 98 Yigal Allon Street, 36 th Floor, ... of Eric Paneth and Izhak Tamir to the ... Rami Skaliter as external directors; , approval of an amendment to ...
(Date:11/24/2015)... , Nov. 24, 2015  Twist Bioscience, ... that Emily Leproust, Ph.D., Twist Bioscience chief executive ... Healthcare Conference on December 1, 2015 at 3:10 ... in New York City. --> ... . Twist Bioscience is on Twitter. Sign ...
(Date:11/24/2015)... ... , ... InSphero AG, the leading supplier of easy-to-use solutions for production, culture, ... serve as Chief Operating Officer. , Having joined InSphero in November 2013 ... was promoted to Head of InSphero Diagnostics in 2014. There she has built ...
(Date:11/24/2015)... Nov. 24, 2015 /CNW Telbec/ - ProMetic Life Sciences Inc. ... that Mr. Pierre Laurin , President and Chief Executive ... the upcoming Piper Jaffray 27 th Annual Healthcare Conference ... December 1-2, 2015. st , at 8.50am ... meetings throughout the day. The presentation will be available live ...
Breaking Biology Technology: