Navigation Links
pTriEx™-4 DNA from Novagen

ProductspTriEx™-4 DNA from Novagen
Company Novagen
Item pTriEx™-4 DNA
Price $228.00
Description The pTriEx™ vectors combine the proven features of the pET, BacVector® and mammalian systems and provides all of the benefits and versatility of each expression system in one vector backbone. The pTriEx vectors have been extensively tested for performance with various target genes in all three expression systems. In each case, expression was similar to that obtained with system-specific vectors. The pTriEx™-4 vector1 is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector a single recombinant plasmid can be used to test expression in E. coli, insect and vertebrate cells. Transient vertebrate expression is mediated by the CMV immediate early enhancer and promoter2. For expression in insect cells, pTriEx-4 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. Expression can be induced in hosts such as NovaBlue by infecting with λCE6, a phage that constitutively expresses T7 RNA polymerase from the λpL and λlpI promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows IPTG based induction. Native protein can be expressed by cloning into the NcoI site, or native protein can be generated by cloning into the PshAI or SmaI sites and cleavage of the fusion protein with Enterokinase or Thrombin, respectively. The pTriEx-4 vector is also available as an Ek/LIC Cloning Kit.
1 patent pending
2 The CMV promoter is covered by U.S. Patent nos. 5,168,062 and 5,385,839 issued to the University of Iowa Research Foundation and is licensed for research use only.
Info NovagenNovagen
441 Charmany Drive
Madison, WI 53719
Customer Service: 800-526-7319
Tech Support: 800-207-0144
Fax Number: 608-238-1388
Web Site: http://www.novagen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. S-Protein Agarose from Novagen
2. STag Thrombin Purification Kit from Novagen
3. S-Protein Agarose from Novagen
4. S-Protein Agarose from Novagen
5. pET GST Fusion System 42 plus Competent Cells from Novagen
6. pET GST Fusion System 42 from Novagen
7. pET GST Fusion System 41 plus Competent Cells from Novagen
8. pET GST Fusion System 41 from Novagen
9. GST•Tag™ Antibody Plate from Novagen
10. Mouse Anti-GST Tag Monoclonal Antibody, Unconjugated from Novagen
11. pETcoco-1 System from Novagen
... protein assay reagents package is used for determining ... and detergents. The RC DC protein assay is ... for 500 standard assays: 250 ml RC reagent ... alkaline copper tartrate solution, 2 L dilute Folin ...
... Kit is used in conjunction with the EasyCount ... automatically. The ViaSure Staining Reagent uses a ... Orange and Ethidium Bromide to identify live and ... the EasyCount Slide 6 Multi-well Slide provides ...
... Protein Precipitation Kit is designed for ... from aqueous samples. Proteomics sample analysis ... of non-protein impurities such as buffers, ... sample that interfere with electrophoretic separations ...
... Trans-Blot cell and PowerPac Basic power supply are ... The Mini Trans-Blot cell accommodates two 7.5 x ... cassettes, modular electrode assembly, Bio-Ice cooling unit, buffer ... pads. The PowerPac basic power supply, 100-120 and ...
Biology Products:
(Date:3/14/2016)... March 14, 2016 NXTD ) ("NXT-ID" ... commerce market, announces the airing of a new series of ... week of March 21 st .  The commercials will air ... popular Squawk on the Street show. --> NXTD ... growing mobile commerce market, announces the airing of a new ...
(Date:3/10/2016)... PUNE, India , March 10, 2016 ... to a new market research report "Identity and Access ... SSO, & Audit, Compliance, and Governance), by Organization Size, ... Forecast to 2020", published by MarketsandMarkets, The market is ... to USD 12.78 Billion by 2020, at a Compound ...
(Date:3/9/2016)... March 9, 2016  Crossmatch ® , a ... solutions, today announced the addition of smart features ... multi-factor authentication platform. New contextual and application-specific ... step-up security where it,s needed most — while ... DC . --> Washington, ...
Breaking Biology News(10 mins):
(Date:4/29/2016)... 2016 According to a ... "Separation Systems for Commercial Biotechnology Market - Global ... 2015 - 2023", the separation systems for commercial ... in 2014 and is projected to expand at ... to reach US$ 19,227.8 Mn in 2023. ...
(Date:4/29/2016)... -- Elekta is pleased to announce that ... treatment planning software, is available for clinical release. Real-world ... version 5.11 provides significant performance speed enhancements over prior ... four times faster than in previous versions of ... Monte Carlo algorithm, users can ...
(Date:4/28/2016)... ... April 28, 2016 , ... Next week on ... on its first-in-class technologies for tissue stem cell counting and expansion to gene-editing ... to Reprogramming & CRISPR-based Genome Engineering in Burlington, Massachusetts. , The attention of ...
(Date:4/27/2016)... ... April 27, 2016 , ... Shimadzu Scientific Instruments ... Spring 2016 Marijuana Business Conference and Expo. Shimadzu’s high-performance instruments enable laboratories to ... more. Expo attendees can stop by booth 1021 to learn how Shimadzu’s instruments ...
Breaking Biology Technology: