Navigation Links
pMIR-hU6-Luc, Human U6 Luciferase Control Vector from Mirus Bio Corporation

ProductspMIR-hU6-Luc, Human U6 Luciferase Control Vector from Mirus Bio Corporation
Company Mirus Bio Corporation
Item pMIR-hU6-Luc, Human U6 Luciferase Control Vector
Price $147.00
Description The potential applications for the use of small interfering RNA (siRNA) molecules to silence gene expression via the RNA interference (RNAi) pathway have generated tremendous interest in the cellular and molecular biology fields. siRNA for experimental use can be synthesized in vitro, either chemically or enzymatically, and then transfected into the target cells in vitro or in vivo. A convenient alternative to introduce siRNAs into mammalian cells is transient or stable transfection of expression constructs that drive siRNA transcription in the nucleus from RNA Polymerase III promoters. An expression cassette is a DNA construct that includes a complete transcription unit consisting of a promoter, a template sequence to be transcribed by RNA polymerase, and a transcription termination signal. An siRNA expression cassette can be in a linear fragment of DNA, in a plasmid [2,3,5-8], in a viral vector, or integrated into the genome. The expression cassettes that have been used for generating siRNA in mammalian cells have utilized the human U6, human H1, or mouse U6 RNA polymerase III promoters. These promoters drive expression of short RNA transcripts that can become siRNA.

An siRNA molecule is formed from short complementary sense and anti-sense strands of RNA. The sense strand of the siRNA has the same sequence as a short region of the mRNA targeted for RNAi. Each siRNA strand may originate from different transcripts expressed in the same cell or both siRNA strands may be part of a single transcript that folds back to form a short hairpin structure. The expression cassette(s) encoding the siRNA strands can be delivered to cells in the same fragment of DNA (hairpin expression cassette) or on separate fragments of DNA (sense and anti-sense strand expression cassettes).

PCR Expression Cassettes can be cloned using either the Hind III or EcoR I site upstream of the promoter and Xho I or BamH I site downstream of the siRNA and termination sequence. The cloning sites used must be unique in the PCR product. Hind III and Xho I are recommended because these restriction enzymes function with compatible buffers.

Info Mirus Bio CorporationMirus Bio Corporation
505 S. Rosa Rd.
Madison, WI 53719

Call Mirus Bio Corporation to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-438-6816
Customer Service: (888) 530-0801 or (608) 441-2852
Fax Number: (608) 441-2849
Web Site: http://www.mirusbio.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Mouse Anti-Human S-100 alpha/beta chain Monoclonal Antibody, Unconjugated, Clone 8B10 from Santa Cruz Biotechnology, Inc.
2. Rabbit Anti-Human Vitamin K-dependent Protein S (PROS1) Polyclonal Antibody, Unconjugated from Lifespan Biosciences
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
5. Mouse Anti-Human Complement factor B Monoclonal Antibody, Unconjugated, Clone 9B6 from AntibodyShop A/S
6. Mouse Anti-Human Choriongonadotropin (hCG) Monoclonal Antibody, Unconjugated, Clone 5F10 from AntibodyShop A/S
7. Mouse Anti-Human Procollagen type I C-terminal propeptide (PICP) Monoclonal Antibody, Unconjugated from AntibodyShop A/S
8. Mouse Anti-Human Acetylcholinesterase, brain (AChE) Monoclonal Antibody, Unconjugated, Clone 12B4 from AntibodyShop A/S
9. Mouse Anti-Human a1-Antitrypsin (alpha-1 AT) Monoclonal Antibody, Unconjugated, Clone 4E5 from AntibodyShop A/S
10. Human and Mouse RAR Neutralizing Peptide from ABR-Affinity BioReagents
11. Rabbit Anti-Human Sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 7 (SIRT7) Polyclonal Antibody, Unconjugated from Lifespan Biosciences
... Human, Assay, 96 wells. ELISA.Specific for ... complexed MMP-3. No cross-reactivity with MMP-1, -2, ... for use with cell culture supernatant, serum, ... -15 to -30 C. Category: Drug ...
... from different human fetal normal tissues ... available upon request. Customers can ... whole set, and pay 70% of ... Dr. P Set products are for ...
Homo sapiens inhibin, beta B (activin AB beta polypeptide) Antigen: Recombinant Protein(142~254aa)...
Self Closing Forceps...
Biology Products:
(Date:10/31/2014)... WCS study confirms that endangered musk deer still live ... sighting, Species targeted by poachers: Musk deer scent glands ... the October issue of the journal Oryx ... 60 years after its last confirmed sighting, a strange ... forested slopes of northeast Afghanistan according to a research ...
(Date:10/31/2014)... Planting trees and creating green space in cities is good ... ensure biodiversity in built environments, a University of Iowa study ... in an urban area in Iowa, and recorded the abundance ... while there were plenty of the trees, black cherry and ... insects, in this case fruit flies that feed on the ...
(Date:10/31/2014)... team have been studying the behavior of approximately ... for years. They observed that ravens slowly build ... playing. However, they also observed that these affiliative ... Although in about 50 % of the cases ... two affiliating ravens, intervening can be potentially risky ...
Breaking Biology News(10 mins):Strange, fanged deer persists in Afghanistan 2Green spaces don't ensure biodiversity in urban areas 2
... 22, 2011 Los Alamos National Laboratory scientists have won ... the "Oscars of Invention" by the Chicago Tribune, these ... the past year. The winning Laboratory technologies include a ... fluid for oil wells that shrinks when heated, and ...
... Concordians have received prestigious awards from the Natural Sciences ... Nadia Bhuiyan and Yves Glinas are the recipients of ... Bhuiyan, a professor in the Department of Mechanical and ... of Aerospace Design and Innovation, specializes in operations management ...
... athletic fields with sand has been used by turfgrass ... and healthy turf, and using porous sand allows the ... adequate drainage, even in heavy foot traffic. While ... municipalities, conversion to a sand-based system is expensive. Complete ...
Cached Biology News:Los Alamos National Laboratory earns 3 R&D 100 Awards 2Los Alamos National Laboratory earns 3 R&D 100 Awards 3Concordia research accelerated 2Optimal topdressing applications for athletic fields determined 2
(Date:10/31/2014)... 2014 Two separate delegations of ... by Charm Sciences at its Andover, Massachusetts facility ... the US, the participants discussed international systems for ... and process verification, and received hands-on technical training ... the first visit to the United States for ...
(Date:10/31/2014)... The report "Dairy Alternative (Beverage) ... Flavored, Sweetened, Unsweetened), Channel (Supermarket, Health Store, Pharmacy, ... Forecast to 2018," defines and segments the dairy ... of revenue and volume for plant-based dairy alternative ... reach $14 Billion by 2018 with the Asia-Pacific ...
(Date:10/31/2014)... , Oct. 31, 2014  Tavistock Development Co. ... Tavistock Group, celebrated the groundbreaking of the GuideWell ... The 92,000-square-foot, three-story facility is ... Florida designed specifically for life sciences, ... heart of the 650-acre Lake Nona Medical City, ...
(Date:10/30/2014)...  Ardelyx, Inc. (NASDAQ: ARDX ), ... and metabolic diseases, today announced that it will ... November 6, 2014. Following the announcement, the Ardelyx ... and webcast at 4:30pm ET to review the ... The live webcast can be accessed ...
Breaking Biology Technology:Charm Sciences Hosts Executives from Latin America for Dairy Safety Executive Seminar 2Dairy Alternative Beverage Market Projected to Reach $14 Billion by 2018 - Report by MarketsandMarkets 2Dairy Alternative Beverage Market Projected to Reach $14 Billion by 2018 - Report by MarketsandMarkets 3Dairy Alternative Beverage Market Projected to Reach $14 Billion by 2018 - Report by MarketsandMarkets 4GuideWell Innovation Center Groundbreaking Hosted by Tavistock Development Company at Lake Nona Medical City 2GuideWell Innovation Center Groundbreaking Hosted by Tavistock Development Company at Lake Nona Medical City 3Ardelyx to Report Third Quarter 2014 Financial Results on November 6, 2014 2