Navigation Links
pET GST Fusion System 41 plus Competent Cells from Novagen

ProductspET GST Fusion System 41 plus Competent Cells from Novagen
Company Novagen
Item pET GST Fusion System 41 plus Competent Cells
Price $450.00
Description 

Schistosomal glutathione-S-transferase (GST) is commonly used as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTTag fusion proteins can be purified with immobilized glutathione. Gentle elution is achieved with buffers containing reduced glutathione. Quantification of soluble GST fusions is also possible by assaying the transferase activity. In order to further enhance the utility of GST as a fusion tag, Novagen has designed two new multi-frame pET vectors, pET-41a-c(+) and pET-42a-c (+). In addition to featuring the powerful T7lac promoter, these vectors encode the GSTTag (220 aa), proteolytic sites, HisTag (6 aa) and STag (15 aa) sequences.

In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the generation of native proteins with their authentic N-terminal residues.

The HisTag and STag sequences enable alternative protein detection and purification procedures to be performed; for example, when enhancing purity with a separate purification method, or when purifying under denaturing conditions.

1. Smith, D.B. and Johnson, K.S. (1988) Gene 67, 3140.

Info NovagenNovagen
441 Charmany Drive
Madison, WI 53719
Customer Service: 800-526-7319
Tech Support: 800-207-0144
Fax Number: 608-238-1388
Web Site: http://www.novagen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. pET GST Fusion System 42 plus Competent Cells from Novagen
2. pET GST Fusion System 42 from Novagen
3. pET GST Fusion System 41 from Novagen
4. His-Patch ThioFusion Expression System from Invitrogen
5. R.A.P.I.D. System from Idaho Technology Inc.
6. CEQ 8800 Genetic Analysis System from Beckman Coulter
7. ProteomeLab PA 800 Protein Characterization System from Beckman Coulter
8. ProteomeLab PF 2D Protein Fractionation System from Beckman Coulter
9. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
10. CEQ 8000 Genetic Analysis System from Beckman Coulter
11. LCMS-IT-TOF Formula Predictor System from Shimadzu
... For use as a carrier ... precipitation. Physical form: Solution in ... 7.4, 1 mM EDTA Preparation ... Concentration: 10 ...
... White solid.. PACKAGED UNDER INERT GAS. ... reaction is "driven" to completion. A protective agent ... found in many other preparations. Blocks the lethal ... by titration. Contaminants: Heavy metals: ≤1 ...
... of Japan, also named Sendai virus) Envelope ... of molecules by means of membrane fusion. ... Protein, Oligo, cDNA, Antibodies, Vector, and Biomolecule ... EX has a high fusing capability in ...
... Genome ChIP-on-chip Microarray is specifically designed ... cerevisiae) DNA binding proteins by pairing ... microarrays. This set delivers robust hybridization ... true binding events and fewer false ...
Biology Products:
(Date:8/6/2015)... Germany , August 6, 2015 /PRNewswire/ ... SensoMotoric Instruments (SMI) shows the world,s first ... based on Epson,s Moverio BT-200 see-through head mounted display ... new solution, unprecedented quality and efficiency is brought to ... displays. For the first time, professionals and researchers can ...
(Date:8/5/2015)... , Aug. 5, 2015 The biosensors ... growth in applications, penetration into newer sectors, and development ... The global biosensors space has seen the entry of ... market so far. (Photo - http://photos.prnewswire.com/prnh/20150805/255570 ... Sullivan, Analysis of the Global Biosensors Market ...
(Date:7/31/2015)... SHENZHEN , Kina, 31. juli 2015 ... ) bliver afholdt af BGI fra den 22. - 25. ... Konferencen fejrer sin 10-års fødselsdag i år. ... verdens mest indflydelsesrige årlige møder på ,omik,-feltet, og er ... forsamlinger. ICG-10 fokuserer på nylige gennembrud ...
Breaking Biology News(10 mins):SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 2SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 3Innovative Biosensors Incite Use in Non-Traditional Applications 2Innovative Biosensors Incite Use in Non-Traditional Applications 3Innovative Biosensors Incite Use in Non-Traditional Applications 4Den 10. internationale konference om Genomics (ICG-10) åbner i oktober 2
... Early snow melt in the Colorado Rocky Mountains initiates two ... fritillary butterfly, Speyeria mormonia . One effect of snow ... required an understanding of the butterfly,s biology. "This suggests ... will be difficult in some cases due to lack of ...
... resources, published today by the Joint Research Centre (JRC), reveals ... average less than 200 mm freshwater available every year while ... The report ,Current Water Resources in Europe and Africa, shows ... 500 mm for Europe and from less than 0.1 mm ...
... A simple, inexpensive dip-and-dry treatment can convert ordinary ... even the armor-coated spores of microbes like anthrax ... Applied Materials & Interfaces . They describe a ... including make-shift curtains and other protective coatings that protect ...
Cached Biology News:An early spring drives butterfly population declines 2Current water resources in Europe and Africa 2
(Date:9/1/2015)... ... September 01, 2015 , ... Leading ... neuro and pain management space join prominent academics and key investors for Neuro ... Harvard Medical School in Boston. , Neuro Advance Boston focuses on the ...
(Date:9/1/2015)... ... 01, 2015 , ... Research conducted by Synedgen Inc.’s Director ... School of Medicine, Frederick National Laboratory for Cancer Research, and the Centers for ... Access journal. , Christopher Ryan PhD, of Synedgen, and Aparajita Chatterjee, Daniel ...
(Date:8/31/2015)... ... August 31, 2015 , ... ... because of its properties such as low carbon footprints and compostability. These properties ... of petroleum based plastics. On the basis of applications, the market for biodegradable ...
(Date:8/31/2015)... 31, 2015 Research and Markets ( http://www.researchandmarkets.com/research/cbgl72/middle_east_and ... East & Africa Biomedical Sensors Market - Growth, Trends ... The Middle East ... billion by 2018 at a CAGR of 3.26% over ... bring Biomedical sensors that are adaptable to the genetic ...
Breaking Biology Technology:Neuro Advance Boston Brings Together Leading Neuroscience Experts on October 14 at Harvard Medical School 2Synedgen’s Director for Pharmaceutical Manufacturing Publishes New Research 2Synedgen’s Director for Pharmaceutical Manufacturing Publishes New Research 3Biodegradable Plastics Market Growing at 10.8% CAGR to 2020 2Biodegradable Plastics Market Growing at 10.8% CAGR to 2020 3Biodegradable Plastics Market Growing at 10.8% CAGR to 2020 4Biodegradable Plastics Market Growing at 10.8% CAGR to 2020 5Middle East & Africa Biomedical Sensors Market Report 2015 - Growth, Trends & Forecasts to 2020 2