Navigation Links
pET GST Fusion System 41 plus Competent Cells from Novagen

ProductspET GST Fusion System 41 plus Competent Cells from Novagen
Company Novagen
Item pET GST Fusion System 41 plus Competent Cells
Price $450.00
Description 

Schistosomal glutathione-S-transferase (GST) is commonly used as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTTag fusion proteins can be purified with immobilized glutathione. Gentle elution is achieved with buffers containing reduced glutathione. Quantification of soluble GST fusions is also possible by assaying the transferase activity. In order to further enhance the utility of GST as a fusion tag, Novagen has designed two new multi-frame pET vectors, pET-41a-c(+) and pET-42a-c (+). In addition to featuring the powerful T7lac promoter, these vectors encode the GSTTag (220 aa), proteolytic sites, HisTag (6 aa) and STag (15 aa) sequences.

In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the generation of native proteins with their authentic N-terminal residues.

The HisTag and STag sequences enable alternative protein detection and purification procedures to be performed; for example, when enhancing purity with a separate purification method, or when purifying under denaturing conditions.

1. Smith, D.B. and Johnson, K.S. (1988) Gene 67, 3140.

Info NovagenNovagen
441 Charmany Drive
Madison, WI 53719
Customer Service: 800-526-7319
Tech Support: 800-207-0144
Fax Number: 608-238-1388
Web Site: http://www.novagen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. pET GST Fusion System 42 plus Competent Cells from Novagen
2. pET GST Fusion System 42 from Novagen
3. pET GST Fusion System 41 from Novagen
4. His-Patch ThioFusion Expression System from Invitrogen
5. R.A.P.I.D. System from Idaho Technology Inc.
6. CEQ 8800 Genetic Analysis System from Beckman Coulter
7. ProteomeLab PA 800 Protein Characterization System from Beckman Coulter
8. ProteomeLab PF 2D Protein Fractionation System from Beckman Coulter
9. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
10. CEQ 8000 Genetic Analysis System from Beckman Coulter
11. LCMS-IT-TOF Formula Predictor System from Shimadzu
HIF-1 beta (ARNT1) Purified Anti-Mouse, Anti-Rat, Anti-Human clone 29, Isotype Mouse IgG 1 , 50 µg Consult technical datasheet for details....
Anti-TWIK-1 Immunogen: Purified peptide from amino acids 53-69 of human TWIK-1 (Accession number O00180)....
This antibody detects an ~92 kDa protein corresponding to the apparent molecular mass of the beta subunit of Hypoxia Inducible Factor-1 (HIF-1beta) on Western blots....
Complexin-1/2 (FL-134)...
Biology Products:
(Date:4/11/2017)... NXT-ID, Inc. (NASDAQ:   NXTD ) ("NXT-ID" or ... independent Directors Mr. Robin D. Richards and Mr. ... the company,s corporate governance and expertise. ... Gino Pereira , Chief Executive Officer said," ... and benefiting from their considerable expertise as we move forward ...
(Date:4/5/2017)... , April 5, 2017 Today ... announcing that the server component of the HYPR platform ... for providing the end-to-end security architecture that empowers biometric ... HYPR has already secured over 15 million users ... including manufacturers of connected home product suites and physical ...
(Date:4/3/2017)... 2017  Data captured by IsoCode, IsoPlexis ... a statistically significant association between the potency ... and objective response of cancer patients post-treatment. ... whether cancer patients will respond to CAR-T ... as to improve both pre-infusion potency testing and ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... BioMedGPS announces expanded ... of its newest module, US Hemostats & Sealants. , SmartTRAK’s US Market for ... fibrin sealants, synthetic sealants and biologic sealants used in surgical applications. BioMedGPS estimates ...
(Date:10/11/2017)... ... 2017 , ... Personal eye wash is a basic first aid supply for any work environment, ... eye do you rinse first if a dangerous substance enters both eyes? It’s one less ... with its unique dual eye piece. , “Whether its dirt and debris, or an acid ...
(Date:10/11/2017)... ... October 11, 2017 , ... Disappearing forests and increased emissions are the main ... people each year. Especially those living in larger cities are affected by air pollution ... of the most pollution-affected countries globally - decided to take action. , “I knew ...
(Date:10/10/2017)... ... October 10, 2017 , ... For the second ... a US2020 STEM Mentoring Award. Representatives of the FirstHand program travelled to Washington, ... from US2020. , US2020’s mission is to change the trajectory of STEM education ...
Breaking Biology Technology: