Navigation Links
pET GST Fusion System 41 plus Competent Cells from Novagen

ProductspET GST Fusion System 41 plus Competent Cells from Novagen
Company Novagen
Item pET GST Fusion System 41 plus Competent Cells
Price $450.00
Description 

Schistosomal glutathione-S-transferase (GST) is commonly used as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTTag fusion proteins can be purified with immobilized glutathione. Gentle elution is achieved with buffers containing reduced glutathione. Quantification of soluble GST fusions is also possible by assaying the transferase activity. In order to further enhance the utility of GST as a fusion tag, Novagen has designed two new multi-frame pET vectors, pET-41a-c(+) and pET-42a-c (+). In addition to featuring the powerful T7lac promoter, these vectors encode the GSTTag (220 aa), proteolytic sites, HisTag (6 aa) and STag (15 aa) sequences.

In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the generation of native proteins with their authentic N-terminal residues.

The HisTag and STag sequences enable alternative protein detection and purification procedures to be performed; for example, when enhancing purity with a separate purification method, or when purifying under denaturing conditions.

1. Smith, D.B. and Johnson, K.S. (1988) Gene 67, 3140.

Info NovagenNovagen
441 Charmany Drive
Madison, WI 53719
Customer Service: 800-526-7319
Tech Support: 800-207-0144
Fax Number: 608-238-1388
Web Site: http://www.novagen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. pET GST Fusion System 42 plus Competent Cells from Novagen
2. pET GST Fusion System 42 from Novagen
3. pET GST Fusion System 41 from Novagen
4. His-Patch ThioFusion Expression System from Invitrogen
5. R.A.P.I.D. System from Idaho Technology Inc.
6. CEQ 8800 Genetic Analysis System from Beckman Coulter
7. ProteomeLab PA 800 Protein Characterization System from Beckman Coulter
8. ProteomeLab PF 2D Protein Fractionation System from Beckman Coulter
9. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
10. CEQ 8000 Genetic Analysis System from Beckman Coulter
11. LCMS-IT-TOF Formula Predictor System from Shimadzu
... Anti-phospho-Ser/Thr-Pro, MPM-2, agarose conjugate ... sodium azide Quality ... immunoprecipitating mitotic MPM2 antigens ... HeLa cells as shown ...
Derlin-2 (C-18)...
CBF-B (G-2)...
Mouse monoclonal [164-12-2] to Ampicillin ( Abpromise for all tested applications)....
Biology Products:
(Date:8/27/2019)... ... August 27, 2019 , ... Ambry Genetics ... genetic testing guidelines. In the largest cohort study of its kind, Ambry presents ... patients. , Clinicians use guidelines to inform decisions about which patients should ...
(Date:8/21/2019)... ... 21, 2019 , ... AbSci, a revolutionary synthetic biology company ... of complex protein therapeutics, today announced the appointment of Fred Larimore, PhD, to ... Larimore brings more than 30 years of experience in biopharmaceutical manufacturing and process ...
(Date:8/15/2019)... ... August 15, 2019 , ... Serialization deadlines ... mandates become increasingly complex, and aggregation becomes fully applied in the United States ... best long-term solution. , Until now, most regulations?including the Drug Supply Chain Security ...
Breaking Biology News(10 mins):
(Date:9/11/2019)... Pa. (PRWEB) , ... September 09, 2019 , ... ... Response Technology (IRT) for clinical trial randomization and drug supply chain management, today ... provider for the global life sciences industry. , “The integration between Veeva Vault ...
(Date:9/9/2019)... ... ... Visikol CEO Dr. Michael Johnson recently gave a talk at the ... cell culture models. The inherent problem of characterizing 3D cell culture models is similar ... and therefore traditional wide-field or even confocal microscopy only tells you what is going ...
(Date:9/4/2019)... ... September 03, 2019 , ... ... delivery technologies, development, and manufacturing solutions for drugs, biologics, gene therapies, and ... Technology will present at the upcoming Nordic Life Science Days (NLS Days) ...
(Date:8/29/2019)... NEW CENTURY, Kan. (PRWEB) , ... August 28, ... ... baking innovation expertise and wide-ranging DuPont™ Danisco® ingredient solutions to attendees at the ... cutting-edge science to solve today’s baking challenges from optimizing production efficiency to cost ...
Breaking Biology Technology: