Navigation Links
pET GST Fusion System 41 plus Competent Cells from Novagen

ProductspET GST Fusion System 41 plus Competent Cells from Novagen
Company Novagen
Item pET GST Fusion System 41 plus Competent Cells
Price $450.00
Description 

Schistosomal glutathione-S-transferase (GST) is commonly used as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTTag fusion proteins can be purified with immobilized glutathione. Gentle elution is achieved with buffers containing reduced glutathione. Quantification of soluble GST fusions is also possible by assaying the transferase activity. In order to further enhance the utility of GST as a fusion tag, Novagen has designed two new multi-frame pET vectors, pET-41a-c(+) and pET-42a-c (+). In addition to featuring the powerful T7lac promoter, these vectors encode the GSTTag (220 aa), proteolytic sites, HisTag (6 aa) and STag (15 aa) sequences.

In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the generation of native proteins with their authentic N-terminal residues.

The HisTag and STag sequences enable alternative protein detection and purification procedures to be performed; for example, when enhancing purity with a separate purification method, or when purifying under denaturing conditions.

1. Smith, D.B. and Johnson, K.S. (1988) Gene 67, 3140.

Info NovagenNovagen
441 Charmany Drive
Madison, WI 53719
Customer Service: 800-526-7319
Tech Support: 800-207-0144
Fax Number: 608-238-1388
Web Site: http://www.novagen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. pET GST Fusion System 42 plus Competent Cells from Novagen
2. pET GST Fusion System 42 from Novagen
3. pET GST Fusion System 41 from Novagen
4. His-Patch ThioFusion Expression System from Invitrogen
5. R.A.P.I.D. System from Idaho Technology Inc.
6. CEQ 8800 Genetic Analysis System from Beckman Coulter
7. ProteomeLab PA 800 Protein Characterization System from Beckman Coulter
8. ProteomeLab PF 2D Protein Fractionation System from Beckman Coulter
9. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
10. CEQ 8000 Genetic Analysis System from Beckman Coulter
11. LCMS-IT-TOF Formula Predictor System from Shimadzu
... Fernbach-style culture flasks have triple baffles located ... to achieve maximal oxygen transfer to culture ... Delong-style 38mm neck for use with plastic ... see Nos. CLS2679638 or CLS2679738. These flasks ...
... is designed to allow neomycin/kanamycin selection in ... template encodes the neomycin/kanamycin resistance gene which ... of kanamycin resistance in E.coli ... of neomycin resistance in mammalian cells. ...
... 5mm diameter, 7 inch long PYREX NMR tubes ... with ground joints, valves, and vacuum racks. They ... from 300 to 500MHz. They have an O.D. ... 4.20 + 0.013-0.000mm. These NMR tubes can be ...
Preparation TW--Compatibility: Push-on end cap seals suitable up to 70 C only -Hybridization Tube for Model HB-1D -Comp Dim: 200 80 mm -Material: borosilicate glass -Mfr Desig: Techne FHB-12...
Biology Products:
(Date:1/24/2020)... ... 2020 , ... Worldwide Business with kathy ireland® has announced an ... President of Sales and Marketing Jeff Maier to discuss their innovative technology, High-Speed Vibration ... Jan. 26, 2020 at 5:30 p.m. EST and internationally on Bloomberg Television on Saturday, ...
(Date:1/22/2020)... COSTA MESA, Calif. (PRWEB) , ... January 21, ... ... appointment of Robert Pozil to lead the company’s new Business Development Department. The ... 4, 2019. Mr. Pozil had been previously serving as the company’s Senior Vice ...
(Date:1/8/2020)... ... January 08, 2020 , ... ... offering complimentary overnight shipping to its nationwide practice network partners. Not only ... due to volume in its Network the biologics themselves are less expensive. , ...
Breaking Biology News(10 mins):
(Date:1/22/2020)... ... January 21, 2020 , ... QCDx , a private biotech company that ... draw, today announced the sale of the proprietary RareScope™ instrument to the Carole and ... in clinical cancer research. , “The RareScope can detect and isolate intact and live ...
(Date:1/10/2020)... (PRWEB) , ... January 09, 2020 , ... ... China, today announced its inaugural GenScript Biotech Global Forum on Jan. 14 in ... the theme "Cell and Gene Therapy and the Booming China Market," will feature ...
(Date:1/7/2020)... , ... January 07, 2020 , ... ... products and services, today announced the launch of its VivopureX™ recombinant mouse antibodies ... clones, many originally obtained from rats or hamsters, which Absolute Antibody has engineered ...
(Date:1/7/2020)... ... January 06, 2020 , ... ... expansion into whole-home smart lighting with two new products: the Brilliant Smart Dimmer ... for homeowners and homebuilders to affordably add high-end smart lighting to any room ...
Breaking Biology Technology: